scholarly journals Elimination of Citrus Tristeza Virus from Triploid Pummelo Cultivar [Citrus maxima (Burm.) Merr.] by In Vivo Micrografting on Seedlings of Three Citrus Cultivars

2021 ◽  
Vol 66 (1) ◽  
pp. 29-35
Author(s):  
Minh–Ly LE ◽  
Kaori SAKAI ◽  
Yuki MIZUNOE ◽  
Kohei KAJIWARA ◽  
Sayuri KAJIHARA ◽  
...  
2020 ◽  
Vol 33 (6) ◽  
pp. 859-870 ◽  
Author(s):  
Thi Nguyet Minh Dao ◽  
Sung-Hwan Kang ◽  
Aurélie Bak ◽  
Svetlana Y. Folimonova

The RNA genome of citrus tristeza virus (CTV), one of the most damaging viral pathogens of citrus, contains 12 open reading frames resulting in production of at least 19 proteins. Previous studies on the intraviral interactome of CTV revealed self-interaction of the viral RNA-dependent RNA polymerase, the major coat protein (CP), p20, p23, and p33 proteins, while heterologous interactions between the CTV proteins have not been characterized. In this work, we examined interactions between the p33 protein, a nonconserved protein of CTV, which performs multiple functions in the virus infection cycle and is needed for virus ability to infect the extended host range, with other CTV proteins shown to mediate virus interactions with its plant hosts. Using yeast two-hybrid, bimolecular fluorescence complementation, and coimmunoprecipitation assays, we demonstrated that p33 interacts with three viral proteins, i.e., CP, p20, and p23, in vivo and in planta. Coexpression of p33, which is an integral membrane protein, resulted in a shift in the localization of the p20 and p23 proteins toward the subcellular crude-membrane fraction. Upon CTV infection, the four proteins colocalized in the CTV replication factories. In addition, three of them, CP, p20, and p23, were found in the p33-formed membranous structures. Using bioinformatic analyses and mutagenesis, we found that the N-terminus of p33 is involved in the interactions with all three protein partners. A potential role of these interactions in virus ability to infect the extended host range is discussed.


2014 ◽  
Vol 42 (4) ◽  
pp. 503-508 ◽  
Author(s):  
Amalendu Ghosh ◽  
Amrita Das ◽  
Ramu Meena ◽  
V. K. Baranwal

Virology ◽  
1994 ◽  
Vol 201 (1) ◽  
pp. 178-181 ◽  
Author(s):  
Vicente J. Febres ◽  
Hanumantha R. Pappu ◽  
Edwin J. Anderson ◽  
Sita S. Pappu ◽  
Richard F. Lee ◽  
...  

HortScience ◽  
1999 ◽  
Vol 34 (2) ◽  
pp. 334-335 ◽  
Author(s):  
D.Q. Fang ◽  
M.L. Roose

`Chandler' pummelo [Citrus maxima (Burm.) Merrill] was found to be citrus tristeza virus (CTV)–resistant. The inheritance of this resistance in 84 progeny of two crosses derived from `Chandler' pummelo and trifoliate orange [Poncirus trifoliata (L.) Raf.] was controlled by a single dominant gene designated Ctv2. Progeny analysis of four molecular markers closely linked to the Ctv gene, which confers resistance to CTV in trifoliate orange, demonstrated that Ctv2 was an independently assorting gene from Ctv.


Author(s):  
Asma Najar ◽  
Imen Hamdi ◽  
Souad Mahmoud ◽  
Lassaad Medhioub ◽  
Imed Jaouadi ◽  
...  

1989 ◽  
Vol 16 (3) ◽  
pp. 315-320
Author(s):  
Ruth Marcus ◽  
Hovav Talpaz ◽  
Moshe Bar-Joseph

2006 ◽  
Vol 49 (1) ◽  
pp. 88-96 ◽  
Author(s):  
Dae Hyun Kim ◽  
Hye Kyung Shim ◽  
Jae Wook Hyeon ◽  
Hyeog Mo Kwon ◽  
Kwang Sik Kim ◽  
...  

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