scholarly journals Construction of Henosepilachna Vigintioctomaculata cDNA Library and Identification of dsRNA Injection–Induced RNAi Effect

2020 ◽  
Vol 65 (1) ◽  
pp. 79-95
Author(s):  
Jeong Hee KIM ◽  
Hye Ri KWON ◽  
Yong Man YU ◽  
Chisa YASUNAGA–AOKI ◽  
Young Nam YOUN
Keyword(s):  
Cdna Library ◽  
Dsrna Injection ◽  
Rnai Effect

Analysis of cDNA library of Cucumis sativus L. challenged by Pseudomonas syringae pv. Lachrymans

2009 ◽  
Vol 31 (10) ◽  
pp. 1042-1048
Author(s):  
Guan-Jun LIU ◽  
Li-Juan WANG ◽  
Zhi-Wei QIN ◽  
Ling-Bo MENG
Keyword(s):  
Cdna Library ◽  
Cucumis Sativus ◽  
Pseudomonas Syringae

Establishment and Identification of a Normalized Full-Length cDNA Library of CCRI 36

2009 ◽  
Vol 35 (4) ◽  
pp. 602-607 ◽  
Author(s):  
Dong WU ◽  
Jun-Jie LIU ◽  
Shu-Xun YU ◽  
Shu-Li FAN ◽  
Mei-Zhen SONG
Keyword(s):  
Cdna Library ◽  
Full Length ◽  
Full Length Cdna

scholarly journals Prediction and Activity of a Cationic α-Helix Antimicrobial Peptide ZM-804 from Maize

2021 ◽  
Vol 22 (5) ◽  
pp. 2643
Author(s):  
Mohamed F. Hassan ◽  
Abdelrahman M. Qutb ◽  
Wubei Dong
Keyword(s):  
Cdna Library ◽  
Pseudomonas Syringae ◽  
Inbred Line ◽  
Mammalian Cells ◽  
Laser Scanning ◽  
Pathogenic Bacteria ◽  
Antimicrobial Activities ◽  
Confocal Laser ◽  
In Planta ◽  
Minimal Inhibitory Concentrations

Antimicrobial peptides (AMPs) are small molecules consisting of less than fifty residues of amino acids. Plant AMPs establish the first barrier of defense in the innate immune system in response to invading pathogens. The purpose of this study was to isolate new AMPs from the Zea mays L. inbred line B73 and investigate their antimicrobial activities and mechanisms against certain essential plant pathogenic bacteria. In silico, the Collection of Anti-Microbial Peptides (CAMPR3), a computational AMP prediction server, was used to screen a cDNA library for AMPs. A ZM-804 peptide, isolated from the Z. mays L. inbred line B73 cDNA library, was predicted as a new cationic AMP with high prediction values. ZM-804 was tested against eleven pathogens of Gram-negative and Gram-positive bacteria and exhibited high antimicrobial activities as determined by the minimal inhibitory concentrations (MICs) and the minimum bactericidal concentrations (MBCs). A confocal laser scanning microscope observation showed that the ZM-804 AMP targets bacterial cell membranes. SEM and TEM images revealed the disruption and damage of the cell membrane morphology of Clavibacter michiganensis subsp. michiganensis and Pseudomonas syringae pv. tomato (Pst) DC3000 caused by ZM-804. In planta, ZM-804 demonstrated antimicrobial activity and prevented the infection of tomato plants by Pst DC3000. Moreover, four virulent phytopathogenic bacteria were prevented from inducing hypersensitive response (HR) in tobacco leaves in response to low ZM-804 concentrations. ZM-804 exhibits low hemolytic activity against mouse red blood cells (RBCs) and is relatively safe for mammalian cells. In conclusion, the ZM-804 peptide has a strong antibacterial activity and provides an alternative tool for plant disease control. Additionally, the ZM-804 peptide is considered a promising candidate for human and animal drug development.

scholarly journals Identification of some unknown transcripts from SSH cDNA library of buffalo follicular oocytes

animal ◽  
2013 ◽  
Vol 7 (3) ◽  
pp. 446-454 ◽  
Author(s):  
S.K. Rajput ◽  
P. Kumar ◽  
B. Roy ◽  
A. Verma ◽  
H.P. Pandey ◽  
...  
Keyword(s):  
Cdna Library

scholarly journals High throughput transformation of a Sorghum cDNA library for rice improvement

2016 ◽  
Vol 125 (3) ◽  
pp. 471-478 ◽  
Author(s):  
Kezhou Qin ◽  
Ping Qiu ◽  
Jianyu Wen ◽  
Yingguo Zhu ◽  
Nengwu Li ◽  
...  
Keyword(s):  
Cdna Library ◽  
High Throughput ◽  
Rice Improvement

scholarly journals Mouse protein arrays from a TH1 cell cDNA library for antibody screening and serum profiling

Genomics ◽  
2005 ◽  
Vol 85 (3) ◽  
pp. 285-296 ◽  
Author(s):  
Claudia Gutjahr ◽  
Derek Murphy ◽  
Angelika Lueking ◽  
Andrea Koenig ◽  
Michal Janitz ◽  
...  
Keyword(s):  
Cdna Library ◽  
Th1 Cell ◽  
Protein Arrays ◽  
Antibody Screening ◽  
Serum Profiling ◽  
Mouse Protein
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