scholarly journals Pollen Tube Growth and Embryo Development in Interspecific Crosses of Cucurbita

10.5109/23809 ◽  
1985 ◽  
Vol 30 (1) ◽  
pp. 1-8
Author(s):  
Soo Nyeon Kwack ◽  
Kunimitsu Fujieda
Keyword(s):  
Pollen Tube ◽  
Embryo Development ◽  
Pollen Tube Growth ◽  
Tube Growth ◽  
Interspecific Crosses

scholarly journals Pollen Tube Growth in Interspecific Crosses between Capsicum Species

HortScience ◽  
1991 ◽  
Vol 26 (5) ◽  
pp. 585-586 ◽  
Author(s):  
Sierd Zijlstra ◽  
Coen Purimahua ◽  
Pim Lindhout
Keyword(s):  
Pollen Tube ◽  
Capsicum Annuum ◽  
Pollen Tube Growth ◽  
Pollen Tubes ◽  
Tube Growth ◽  
Egg Cell ◽  
Interspecific Crosses ◽  
Capsicum Species ◽  
Pollen Tube Penetration ◽  
Capsicum Chacoense

Crossing barriers between white- and purple-flowered species were examined. Four accessions of Capsicum annuum and three of C. pubescens were reciprocally crossed with one to four accessions of C. baccatum, C. cardenasii, C. chacoense, C. chinense, C. eximium, C. frutescens, C. galapagoense, and C. praetermissum. Capsicum chacoense is the only white-flowered species that inhibits C. annuum pollen tube growth but allows C. pubescens pollen tube penetration into the egg cell. Capsicum cardenasii and C. eximium exhibit similar crossabilities with C. annuum and C. pubescens: pollen tubes of C. cardenasii and of C. eximium can penetrate the egg cells of C. annuum but not vice versa, and pollen tubes of C. pubescens can penetrate the egg cells of C. cardenasii and of C. eximium but not vice versa.

scholarly journals Reproductive ecology of buzz-pollinated <i>Ouratea spectabilis</i> trees (Ochnaceae) in Brazilian Cerrados

Web Ecology ◽  
2015 ◽  
Vol 14 (1) ◽  
pp. 79-84 ◽  
Author(s):  
D. Montesinos ◽  
P. Oliveira
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Viability ◽  
Reproductive Ecology ◽  
Fruit Production ◽  
Tube Growth ◽  
Evolutionary Strategy ◽  
Interspecific Crosses ◽  
Pollination Syndrome ◽  
Self Pollination

Abstract. Ouratea spectabilis is a ubiquitous tree species in the Brazilian savannas, or Cerrados, where it plays an important ecological role. We studied its anthesis phenology, pollination biology, pollen viability, and pollen tube growth, and executed a set of intra- and interspecific experimental crosses to determine its mechanisms of incompatibility and reproductive ecology. The species presents a specialized buzz pollination syndrome and is served by a small array of specialized pollinator species. It is a mostly self-incompatible species, and although self-pollination is possible, it strongly reduces fertility, with reproductive outputs for hand self-pollination similar to those of interspecific crosses with the co-generic species O. hexasperma. Incompatibility with another commonly co-occurring co-generic species, O. floribunda, was complete, with a null fruit set, as occurred for the autonomous apomixis tests. Our pollen tube growth observations indicate that incompatibility occurs at the style, and is thus pre-zygotic. All three Ouratea species presented very high pollen viability. Manual pollen supplementation did not increase seed sets. Nevertheless, even after excess manual pollen supplementation, seed-to-ovule ratios were only 30%. Such limits are common in stressful environments, and fruit production for most Cerrado species is reported to be regularly under those levels. The apparent ubiquity of this fertility limit among Cerrado species poses interesting ecological questions, such as the role of environmental stress on reproduction and the potential overproduction of ovules as an evolutionary strategy to deal with seed predation – questions which deserve further research in the future.

scholarly journals Pollen Tube Growth, Fertilization and Embryo Development of Camellia japonica L.*C. chrysantha (Hu) Tuyama.

1992 ◽  
Vol 60 (4) ◽  
pp. 955-961 ◽  
Author(s):  
Yueh-Jiang Hwang ◽  
Hiroshi Okubo ◽  
Kunimitsu Fujieda
Keyword(s):  
Pollen Tube ◽  
Embryo Development ◽  
Pollen Tube Growth ◽  
Tube Growth ◽  
Camellia Japonica

Pollination, Fertilization, and Embryo Development in Southern China Fresh-eating Jujube

HortScience ◽  
2020 ◽  
pp. 1-8
Author(s):  
Fengxia Shao ◽  
Sen Wang ◽  
Zhiming Liu ◽  
Rongyan Hong ◽  
Tianjiao Zhao
Keyword(s):  
Pollen Tube ◽  
Embryo Development ◽  
Pollen Tube Growth ◽  
Southern China ◽  
Pollen Tubes ◽  
Tube Growth ◽  
Seed Abortion ◽  
Cross Pollination ◽  
Globular Embryos ◽  
Pollination And Fertilization

To explore the reasons for seed abortion in southern China fresh-eating jujube, improve its reproductive biology, and provide a theoretical basis for the crossbreeding of jujube, we carried out self-pollination and cross-pollination experiments with Ziziphus jujuba Mill. ‘Zhongqiusucui’ as the female parent. We observed the process of pollen tube growth in pistil and embryo development by fluorescence microscopy and paraffin section methods. The results show there were self- and cross-incompatibilities during pollination and fertilization, and there were no significant differences in pollen germination and pollen tube growth between self-pollination and cross-pollination. It took at least 4 hours for pollen and stigma to recognize each other, 6 hours for pollen to germinate on the stigma, and 12 hours for the pollen tube to penetrate the mastoid cells of the stigma. After 48 hours of pollination, the pollen tube reached one third of the style. The pollen tube remained stagnant 72 to 120 hours after pollination, and remained at one third of the stylar canal. Simultaneously, the pollen tubes on the stigma twisted and interacted with each other, and expanded into a spherical shape. A few pollen tubes reached the ovary and completed fertilization. However, some early globular embryos degenerated before forming into globular embryos and resulted in the formation of empty embryo sacs, which leads to seed abortion. In conclusion, the poor pollination and fertilization, and the blocked development of the embryo resulted in seed abortion in Z. jujuba ‘Zhongqiusucui’.

Interspecific pollen–pistil incongruity in Salix

1989 ◽  
Vol 19 (9) ◽  
pp. 1161-1168 ◽  
Author(s):  
A. Mosseler
Keyword(s):  
Growth Rate ◽  
North America ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Tube Growth ◽  
Interspecific Crosses ◽  
Pollen Tube Growth Rate ◽  
Pollen Tube Penetration ◽  
Foreign Species ◽  
Salix Spp

Pollination barriers that restrict hybridization between six Salix spp. (S. amygdaloides Anderss., S. exigua Nutt., and S.lucida Muhl., of subgenus Salix, and S. discolor Muhl., S. eriocephala Michx., and S. petiolaris Smith of subgenus Vetrix) and that are sympatric over much of their ranges in central North America were investigated through artificial cross pollination. Foreign species' pollen generally adhered to and germinated on the stigma. Pollen–pistil incongruity was most often expressed as reduced pollen tube growth rate, but the degree of incongruity was highly variable within and between different species combinations. Morphological abnormalities such as swollen, coiled, and undirected pollen tube growth were observed in some crosses but were not common. The stigma of S. eriocephala Michx. exhibited a particularly strong and characteristic inhibition of all foreign species' pollen tube growth. Despite the presence of some form of pollen–pistil incongruity in most interspecific crosses, pollen tube penetration of the ovule micropyle (fertilization) was successful in several species combinations.

An SEM Study of Pollen Tube Growth in Intra- and Interspecific Crosses between Lilium Species

1988 ◽  
Vol 149 (4) ◽  
pp. 365-369 ◽  
Author(s):  
P. M. van Roggen ◽  
C. J. Keijzer ◽  
H. J. Wilms ◽  
J. M. van Tuyl
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Tube Growth ◽  
Interspecific Crosses ◽  
Lilium Species ◽  
Sem Study

BARRIERS TO HYBRIDIZATION OF TRIFOLIUM REPENS WITH RELATED SPECIES

1972 ◽  
Vol 14 (2) ◽  
pp. 381-389 ◽  
Author(s):  
Chi-Chang Chen ◽  
Pryce B. Gibson
Keyword(s):  
Pollen Tube ◽  
Trifolium Repens ◽  
Related Species ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Pollen Tubes ◽  
Tube Growth ◽  
Interspecific Crosses ◽  
Viable Seeds ◽  
Time Required

Pre-fertilization barriers to hybridization of Trifolium repens L. with its related species, T. nigrescens, T. occidentale, T. hybridum, T. ambiguum, and T. uniflorum, were studied by comparing pollen germination, pollen-tube growth, and fertilization following intra- and interspecific pollinations on T. repens. Pollen of T. repens began to germinate almost immediately after being placed on the stigmas of its own species; pollen germination approached 100% in 30 min. Pollen tubes grew rapidly. Eight hours after pollination the longer tubes had entered the micropyle of the most distant ovules. Fertilization was first observed at 8 hr. All ovules were fertilized within 24 hr. In interspecific pollinations using T. repens as the pistillate parent, the time required for pollen germination was longer and the frequency of germination was lower. Pollen tubes frequently swelled, coiled, or even burst in the styles. Pollen tubes of the species more closely related to T. repens appeared to grow faster and more normally. Fertilization occurred in all interspecific crosses. The highest frequency of ovules fertilized generally occurred in the species combinations in which pollen-tube growth was most similar to the control and the lowest frequency, in the combinations in which tube growth deviated the most from the control. These observations suggest that pre-fertilization barriers are not the only causes of cross-incompatibility of T. repens with related species. Post-fertilization barriers, which prevent zygotes from developing into viable seeds, also must exist.

scholarly journals Pollination, Fertilization, and Embryo Development in Southern China Fresh-eating Jujube

HortScience ◽  
2020 ◽  
Vol 55 (8) ◽  
pp. 1315-1322
Author(s):  
Fengxia Shao ◽  
Sen Wang ◽  
Zhiming Liu ◽  
Rongyan Hong ◽  
Tianjiao Zhao
Keyword(s):  
Pollen Tube ◽  
Embryo Development ◽  
Pollen Tube Growth ◽  
Southern China ◽  
Pollen Tubes ◽  
Tube Growth ◽  
Seed Abortion ◽  
Cross Pollination ◽  
Globular Embryos ◽  
Pollination And Fertilization

To explore the reasons for seed abortion in southern China fresh-eating jujube, improve its reproductive biology, and provide a theoretical basis for the crossbreeding of jujube, we carried out self-pollination and cross-pollination experiments with Ziziphus jujuba Mill. ‘Zhongqiusucui’ as the female parent. We observed the process of pollen tube growth in pistil and embryo development by fluorescence microscopy and paraffin section methods. The results show there were self- and cross-incompatibilities during pollination and fertilization, and there were no significant differences in pollen germination and pollen tube growth between self-pollination and cross-pollination. It took at least 4 hours for pollen and stigma to recognize each other, 6 hours for pollen to germinate on the stigma, and 12 hours for the pollen tube to penetrate the mastoid cells of the stigma. After 48 hours of pollination, the pollen tube reached one third of the style. The pollen tube remained stagnant 72 to 120 hours after pollination, and remained at one third of the stylar canal. Simultaneously, the pollen tubes on the stigma twisted and interacted with each other, and expanded into a spherical shape. A few pollen tubes reached the ovary and completed fertilization. However, some early globular embryos degenerated before forming into globular embryos and resulted in the formation of empty embryo sacs, which leads to seed abortion. In conclusion, the poor pollination and fertilization, and the blocked development of the embryo resulted in seed abortion in Z. jujuba ‘Zhongqiusucui’.

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