scholarly journals A Simple Method of Optical Evaluation of the Distribution of Muscle Extracellular Matrix by Immunofluorescence and Image Analysis using Fluorescent Semiconductor Nanocrystals (quantum dot)

10.5109/14045 ◽  
2009 ◽  
Vol 54 (1) ◽  
pp. 103-108
Author(s):  
Yoshi-Nori Nakamura ◽  
Tomiko Fumita ◽  
Keisuke Hayashi ◽  
Fumio Ebara ◽  
Tetsuji Etoh ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Image Analysis ◽  
Quantum Dot ◽  
Semiconductor Nanocrystals ◽  
Simple Method ◽  
Fluorescent Semiconductor Nanocrystals

Electrochemical Determination of Uric Acid at CdTe Quantum Dot Modified Glassy Carbon Electrodes

2015 ◽  
Vol 98 (5) ◽  
pp. 1260-1266 ◽  
Author(s):  
Deng Pan ◽  
Shengzhong Rong ◽  
Guangteng Zhang ◽  
Yannan Zhang ◽  
Qiang Zhou ◽  
...  
Keyword(s):  
Uric Acid ◽  
Quantum Dot ◽  
Glassy Carbon ◽  
Semiconductor Nanocrystals ◽  
Phosphate Buffer Solution ◽  
Differential Pulse ◽  
Electrochemical Determination ◽  
Buffer Solution ◽  
Peak Current ◽  
Cdte Quantum Dot

Abstract Cyclic voltammetry and differential pulse voltammetry were used to investigate the electrochemical behavior of uric acid (UA) at a CdTe quantum dot (QD) modified the glassy carbon electrode (GCE). CdTe QDs, as new semiconductor nanocrystals, can greatly improve the peak current of UA. The anodic peak current of UA was linear with its concentration between 1.0 × 10–6 and 4.0 × 10–4 M in 0.1 M pH 5.0 phosphate buffer solution. The LOD for UA at the CdTe electrode (1.0 × 10–7 M) was superior to that of the GCE. In addition, we also determined the effects of scan rate, pH, and interferences of UA for the voltammetric behavior and detection. The results indicated that modified electrode possessed excellent reproducibility and stability. Finally, a new and efficient electrochemical sensor for detecting UA was developed.

Measuring Coral Skeletal Architecture Using Image Analysis v1

2021 ◽  
Author(s):  
Rowan Mclachlan ◽  
Ashruti Patel ◽  
Andrea G Grottoli
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Image Analysis ◽  
Scleractinian Coral ◽  
Coral Species ◽  
Energy Materials ◽  
Simple Method ◽  
Coral Skeletons ◽  
Skeletal Structures ◽  
Scanning Electron

Coral morphology is influenced by genetics, the environment, or the interaction of both, and thus is highly variable. This protocol outlines a non-destructive and relatively simple method for measuring Scleractinian coral sub-corallite skeletal structures (such as the septa length, theca thickness, and corallite diameter, etc.) using digital images produced as a result of digital microscopy or from scanning electron microscopy. This method uses X and Y coordinates of points placed onto photomicrographs to automatically calculate the length and/or diameter of a variety of sub-corallite skeletal structures in the Scleractinian coral Porites lobata. However, this protocol can be easily adapted for other coral species - the only difference may be the specific skeletal structures that are measured (for example, not all coral species have a pronounced columella or pali, or even circular corallites). This protocol is adapted from the methods described in Forsman et al. (2015) & Tisthammer et al. (2018). There are 4 steps to this protocol: 1) Removal of Organic Tissue from Coral Skeletons 2) Imaging of Coral Skeletons 3) Photomicrograph Image Analysis 4) Calculation of Corallite Microstructure Size This protocol was written by Dr. Rowan McLachlan and was reviewed by Ashruti Patel and Dr. Andréa Grottoli. Acknowledgments Leica DMS 1000 and Scanning Electron Microscopy photomicrographs used in this protocol were acquired at the Subsurface Energy Materials Characterization and Analysis Laboratory (SEMCAL), School of Earth Sciences at The Ohio State University, Ohio, USA. I would like to thank Dr. Julie Sheets, Dr. Sue Welch, and Dr. David Cole for training me on the use of these instruments.

scholarly journals 3D magnetically controlled spatiotemporal probing and actuation of collagen networks from a single cell perspective

Lab on a Chip ◽  
2021 ◽  
Vol 21 (20) ◽  
pp. 3850-3862
Author(s):  
Daphne O. Asgeirsson ◽  
Michael G. Christiansen ◽  
Thomas Valentin ◽  
Luca Somm ◽  
Nima Mirkhani ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Extracellular Matrix ◽  
Image Analysis ◽  
Matrix Models ◽  
Single Cell ◽  
Physical Modeling ◽  
Single Cells ◽  
Field Control

Rod-shaped magnetic microprobes are employed to assess and actuate extracellular matrix models in 3D from the perspective of single cells. To achieve this, our method combines magnetic field control, physical modeling, and image analysis.

scholarly journals The effects of tamoxifen and estradiol on myometrial differentiation and organization during early uterine development in the CD1 mouse

Reproduction ◽  
2009 ◽  
Vol 138 (2) ◽  
pp. 341-350 ◽  
Author(s):  
Mohamed K Mehasseb ◽  
S C Bell ◽  
M A Habiba
Keyword(s):  
Extracellular Matrix ◽  
Image Analysis ◽  
Steroid Receptors ◽  
Smooth Muscle Actin ◽  
Estrogen Receptor Α ◽  
Matrix Proteins ◽  
Muscle Actin ◽  
Uterine Adenomyosis ◽  
Circular Arrangement ◽  
Uterine Development

We used a neonatal mouse model to examine the histogenesis of uterine adenomyosis, and to test whether adenomyosis is due to an abnormality in myometrial differentiation, or in extracellular matrix proteins expression. We also studied the effects of tamoxifen and estradiol on uterine development, myometrial differentiation, and organization. Female CD1 pups were treated with oral tamoxifen (1 mg/kg) (n=27) or estradiol (0.1 mg/kg) (n=24) from age 1 to 5 days. Uteri from control (n=27) and treated mice were obtained on days 2, 5, 10, 15, and 42 of age. We examined the sections histologically, using image analysis and immunohistochemistry for α-smooth muscle actin (α-SMA), desmin, vimentin, laminin, fibronectin, and estrogen receptor-α. Following tamoxifen exposure, all uteri showed adenomyosis by 6 weeks of age (seen as early as day 10). The inner myometrium showed thinning, lack of continuity, disorganization, and bundling. α-SMA expression was normal. Desmin expression normally showed a wave of maturation that was absent in tamoxifen-treated mice. In the estradiol group, adenomyosis was not observed. All uterine layers were normally developed, but hypertrophied. The inner myometrium retained its circular arrangement. There was no difference in the localization of laminin or fibronectin between groups (laminin expression was reduced in the tamoxifen treated uteri). Vimentin could not be detected in all groups. Our results suggest that the development of the inner myometrium is particularly sensitive to estrogen antagonism, and can be affected by steroid receptors modulation. Disruption of the inner myometrium may play a role in the development of uterine adenomyosis.

scholarly journals Pore system changes of damaged Brazilian oxisols and nitosols induced by wet-dry cycles as seen in 2-D micromorphologic image analysis

2009 ◽  
Vol 81 (1) ◽  
pp. 151-161 ◽  
Author(s):  
Luiz F. Pires ◽  
Klaus Reichardt ◽  
Miguel Cooper ◽  
Fabio A.M. Cássaro ◽  
Nivea M.P. Dias ◽  
...  
Keyword(s):  
Image Analysis ◽  
Size Distribution ◽  
Soil Structure ◽  
Tropical Soils ◽  
Transport Processes ◽  
Structure Characterization ◽  
Control Treatment ◽  
Simple Method ◽  
Wetting And Drying ◽  
Essential Information

Soil pore structure characterization using 2-D image analysis constitutes a simple method to obtain essential information related to soil porosity and pore size distribution (PSD). Such information is important to infer on soil quality, which is related to soil structure and transport processes inside the soil. Most of the time soils are submitted to wetting and drying cycles (W-D), which can cause important changes in soils with damaged structures. This report uses 2-D image analysis to evaluate possible modifications induced by W-D cycles on the structure of damaged soil samples. Samples of three tropical soils (Geric Ferralsol, GF; Eutric Nitosol, EN; and Rhodic Ferralsol, RF) were submitted to three treatments: 0WD, the control treatment in which samples were not submitted to any W-D cycle; 3WD and 9WD with samples submitted to 3 and 9 consecutive W-D cycles, respectively. It was observed that W-D cycles produced significant changes in large irregular pores of the GF and RF soils, and in rounded pores of the EN soil. Nevertheless, important changes in smaller pores (35, 75, and 150 µm) were also observed for all soils. As an overall consideration, it can be said that the use of image analysis helped to explain important changes in soil pore systems (shape, number, and size distribution) as consequence of W-D cycles.

scholarly journals A Very Simple Method for Quickly Making Large Numbers of Measurements on Micrographs

2009 ◽  
Vol 17 (3) ◽  
pp. 50-53
Author(s):  
Ron Anderson
Keyword(s):  
Thin Film ◽  
Grain Size ◽  
Image Analysis ◽  
Integrated Circuit ◽  
Ceramic Substrate ◽  
Raw Material ◽  
Material Particle ◽  
Simple Method ◽  
Plan View ◽  
Large Computer

It has been in the back of my mind to write this up for MT since I retired from a certain large computer company. Inasmuch as Paul's article above is a perfect lead-in, there is no time like the present. Our lab supported a semiconductor integrated circuit and a ceramic substrate manufacturing facility. We were continually required to measure circuit line widths on plan-view specimens and layer thicknesses on cross-section specimens for both semiconductor and ceramic substrate specimens and we were often asked to determine thin film grain size and ceramic raw material particle size data. A large number of measurements were required for each specimen to guarantee statistically sound data. We had image analysis software available that we used whenever we could, but often found that measuring things on a system using grey-level image analysis as input simply did not work. This is especially true for thin film grain size determination when using diffraction contrast TEM images for input.

Simple method to incorporate nonparabolicity effects in the Schrödinger equation of a quantum dot

2007 ◽  
Vol 101 (9) ◽  
pp. 093715 ◽  
Author(s):  
F. M. Gómez-Campos ◽  
S. Rodríguez-Bolívar ◽  
J. E. Carceller
Keyword(s):  
Quantum Dot ◽  
Schrödinger Equation ◽  
Schrodinger Equation ◽  
Simple Method

scholarly journals A Facile Method to Fabricate Anisotropic Extracellular Matrix with 3D Printing Topological Microfibers

Materials ◽  
2019 ◽  
Vol 12 (23) ◽  
pp. 3944 ◽  
Author(s):  
Zhen Gu ◽  
Zili Gao ◽  
Wenli Liu ◽  
Yongqiang Wen ◽  
Qi Gu
Keyword(s):  
Mechanical Properties ◽  
Extracellular Matrix ◽  
Mechanical Characteristics ◽  
Fiber Direction ◽  
Gelatin Matrix ◽  
Simple Method ◽  
Elongation At Break ◽  
Anisotropic Mechanical Properties ◽  
The Matrix ◽  
3D Printed

Natural tissues and organs have different requirements regarding the mechanical characteristics of response. It is still a challenge to achieve biomaterials with anisotropic mechanical properties using an extracellular matrix with biological activity. We have improved the ductility and modulus of the gelatin matrix using 3D printed gelatin microfibers with different concentrations and topologies and, at the same, time achieved anisotropic mechanical properties. We successfully printed flat microfibers using partially cross-linked gelatin. We modified the 10% (w/v) gelatin matrix with microfibers consisting of a gelatin concentration of 14% (w/v), increasing the modulus to about three times and the elongation at break by 39% in parallel with the fiber direction. At the same time, it is found that the microfiber topology can effectively change the matrix ductility, and changing the modulus of the gelatin used in the microfiber can effectively change the matrix modulus. These findings provide a simple method for obtaining active biological materials that are closer to a physiological environment.

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