Quantification of Capillaries in the Papillary Dermis with a Novel Observation Technology and Its Relationship with Skin Aging

2021 ◽  
Vol 55 (1) ◽  
pp. 52-60
Author(s):  
Junichi Mori ◽  
Xiao Ling ◽  
Yoshitaka Yamaguchi ◽  
Naoko Yoshida ◽  
Eriko Inui ◽  
...  
Keyword(s):  
Skin Aging ◽  
Papillary Dermis

scholarly journals In vitro beneficial effects of a flax extract on papillary fibroblasts define it as an anti-aging candidate

2021 ◽  
Vol 5 (1) ◽  
pp. 032-040
Author(s):  
Hervé PAGEON ◽  
Hélène ZUCCHI ◽  
Sylvie RICOIS ◽  
Philippe BASTIEN ◽  
Daniel ASSELINEAU
Keyword(s):  
Mechanical Properties ◽  
Close Contact ◽  
Biological Activities ◽  
Skin Aging ◽  
Papillary Dermis ◽  
Reticular Dermis ◽  
Laminin 5 ◽  
Beneficial Effects ◽  
Reconstructed Skin

Objective: During aging, skin undergoes structural, cellular and molecular changes, which not only alter skin mechanical properties but also biological and physiological functions. Structurally the epidermis becomes thinner, the dermal epidermal junction flattens and the extra-cellular matrix component of the dermis is disorganized and degraded. The dermis is composed of two compartments: The Reticular dermis is the deepest and thickest part while the upper layer, the papillary dermis, which is much thinner and is in close contact with epidermis, plays an important role in the structure and function of the skin. We have recently shown that the papillary dermis was preferentially affected by skin aging because the activity of fibroblasts in this region was especially altered as a function of age. The purpose of this study was to investigate the capacity of a flax extract as anti-aging component. Method: We investigated the capacity of a flax extract to stimulate or restore the activity of papillary fibroblasts from young and old donors in cultured monolayers and in reconstructed skin. Several biological markers of extracellular matrix homeostasis and mechanical properties were investigated. Results: The tested flax extract seemed to improve parameters known to change with age: I/ In monolayers after treatment the number of aged fibroblasts increased II/ In reconstructed skin the flax extract appears to positively regulate some biological activities; particularly in aged fibroblasts where the deposition of laminin 5, fibrillin 1, procollagen I were increased in the dermis and the secretion of specific soluble factors like MMP1, MMP3 and KGF were regulated to levels similar to those observed in young fibroblasts III/ Mechanical properties were improved particularly for elastics parameters (R5, R2 and R7). Conclusion: The flax extract is a promising anti-aging compound. The treatment of aged papillary fibroblasts resulted in a return to a younger-like profile for some of the studied parameters.

Apoptosis: A Role in Skin Aging?

1998 ◽  
Vol 3 (1) ◽  
pp. 28-35 ◽  
Author(s):  
Anne R Haake ◽  
Inna Roublevskaia ◽  
Molly Cooklis
Keyword(s):  
Skin Aging

scholarly journals Anti-Aging Potential of Extracts from Washingtonia filifera Seeds

Plants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 151
Author(s):  
Benedetta Era ◽  
Sonia Floris ◽  
Valeria Sogos ◽  
Clara Porcedda ◽  
Alessandra Piras ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Protective Effect ◽  
Pharmaceutical Preparation ◽  
Human Keratinocytes ◽  
Inhibitory Effect ◽  
Skin Aging ◽  
Stressed Cells ◽  
Collagenase Inhibition ◽  
Washingtonia Filifera ◽  
Seed Extracts

The aim of this study was to test the inhibitory effect of fruit extracts from Washingtonia filifera on skin aging-related enzymes. The pulp extracts did not exert a significant enzyme inhibition while seed extracts from W. filifera exhibit anti-elastase, anti-collagenase, and anti-tyrosinase activities. Tyrosinase was mildly inhibited while a stronger effect was observed with respect to elastase and collagenase inhibition. Alcoholic extracts provided better results than aqueous extracts. Among them, methanol extracts showed the prominent enzyme inhibitory activities being IC50 value for elastase and collagenase comparable and even better than the reference compound. The inhibition mode of the most active extracts was investigated by Lineweaver-Burk plot analysis. Seed extracts from W. filifera were also investigated for their photo-protective effect by Mansur equation and the antioxidant activity of W. filifera extract was evaluated in oxidative-stressed cells. To evaluate the safety of the extract, the effect on cell viability of human keratinocytes cells was analyzed. Methanol extract presented the best photo-protective effect and exerted an antioxidant activity in a cellular system with no cytotoxic effect. The overall results demonstrate that W. filifera extracts are promising sources of bioactive compounds that could be used in cosmetic and pharmaceutical preparation.

Enhanced Skin Permeation of Punicalagin after Topical Application of Pluronic Micelles or Vesicles Loaded with Lafoensia pacari Extract

Planta Medica ◽  
2021 ◽  
Author(s):  
Sandra Alves de Sousa Garcia ◽  
Priscila Bianca Rodrigues da Rocha ◽  
Bruno dos Santos Souza ◽  
Andressa Tuane Santana Paz ◽  
Ana Luiza Caetano Negris ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Skin Permeation ◽  
Sol Gel ◽  
Extraction Procedure ◽  
Average Diameter ◽  
Skin Aging ◽  
Chemical Degradation ◽  
Vertical Diffusion ◽  
Flowing Liquid ◽  
Span 80

AbstractPunicalagin, the principal ellagitannin of Lafoensia pacari leaves, has proven antioxidant activity, and standardized extracts of L. pacari can be topically used for skin aging management. We hypothesized that Pluronic nanomicelles or vesicles could solubilize sufficiently large amounts of the standardized extracts of L. pacari and provide chemical stability to punicalagin. The standardized extracts of L. pacari were obtained with an optimized extraction procedure, and the antioxidant activity was characterized. Formulations containing Pluronic at 25% and 35% were obtained with or without Span 80. They were characterized by average diameter, polydispersity index, punicalagin content, physicochemical stability, and rheology. A release and skin permeation study was carried out in vertical diffusion cells. The extraction procedure allowed quantifying high punicalagin content (i.e., 141.61 ± 3.87 mg/g). The standardized extracts of L. pacari showed antioxidant activity for all evaluated methods. Pluronic at 25 and Pluronic at 35 with standardized extracts of L. pacari showed an average diameter of about 25 nm. The addition of Span 80 significantly increased the mean diameter by 15-fold (p < 0.05), indicating the spontaneous formation of vesicles. Pluronic formulations significantly protected punicalagin from chemical degradation (p < 0.05). Pluronic at 25 formulations presented as free-flowing liquid-like systems, while Pluronic at 35 resulted in an increase of about 44-fold in |ƞ*|. The addition of Span 80 significantly reduced the Pluronic sol-gel transition temperature (p < 0.05), indicating the formation of vesicles. Formulations with Span 80 significantly enhanced punicalagin skin permeation compared to formulations without Span 80 (p < 0.05). Formulations with Span 80 were demonstrated to be the most promising formulations, as they allowed significant permeation of punicalagin (about 80 to 315 µg/cm2), which has been shown to have antioxidant activity.

scholarly journals Protective Effect of Spirulina-Derived C-Phycocyanin against Ultraviolet B-Induced Damage in HaCaT Cells

Medicina ◽  
2021 ◽  
Vol 57 (3) ◽  
pp. 273
Author(s):  
Young Ah Jang ◽  
Bo Ae Kim
Keyword(s):  
Antioxidant Defense System ◽  
Skin Aging ◽  
Ultraviolet B ◽  
Control Group ◽  
Hacat Cells ◽  
Uvb Radiation ◽  
Skin Damage ◽  
Blue Green Algae ◽  
Radiation Group ◽  
Skin Wrinkles

Background and objectives: Reactive oxygen species (ROS) overwhelm the antioxidant defense system, induce oxidative stress, and increase matrix metalloproteinase (MMP) expression, resulting in skin aging. Thus, preventing ultraviolet B (UVB)-induced skin damage can attenuate skin aging. Spirulina (a biomass of cyanobacteria, also called blue-green algae) is comprised of prokaryotes, whereas microalgae are eukaryotes and are rich in phycocyanin, a powerful antioxidant. Materials and Methods: Here, we investigated the photoprotective effects of spirulina-derived C-phycocyanin (C-PC) against UVB radiation using keratinocytes (HaCaT cells). Results: UVB radiation increased MMP-1 and MMP-9 expression but decreased involucrin, filaggrin, and loricrin expression. C-PC showed no toxicity at concentrations of 5–80 μg/mL in terms of HaCaT cell viability. UVB-irradiated HaCaT cells had a 50.8% survival rate, which increased to 80.3% with C-PC treatment. MMP expression increased with UVB treatment, whereas MMP-1 and MMP-9 concentrations decreased with C-PC treatment. UVB reduced involucrin, filaggrin, and loricrin expression in HaCaT cells, but 80 μg/mL C-PC increased their expression by >25%. In the UVB radiation group, dichlorofluorescin diacetate fluorescence intensity in HaCaT cells increased by 81.6% compared with that in the control group, whereas ROS production was reduced by 51.2% and 55.1% upon treatment with 40 and 80 μg/mL C-PC, respectively. Conclusions: C-PC might reduce or prevent skin aging by reducing UVB irradiation-induced skin wrinkles and free radicals.

scholarly journals Multiple-Molecule Drug Design Based on Systems Biology Approaches and Deep Neural Network to Mitigate Human Skin Aging

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3178
Author(s):  
Shan-Ju Yeh ◽  
Jin-Fu Lin ◽  
Bor-Sen Chen
Keyword(s):  
Neural Network ◽  
Systems Biology ◽  
Signaling Pathways ◽  
Human Skin ◽  
Deep Neural Network ◽  
Middle Age ◽  
Skin Aging ◽  
Identification System ◽  
Potential Candidate ◽  
Systems Medicine

Human skin aging is affected by various biological signaling pathways, microenvironment factors and epigenetic regulations. With the increasing demand for cosmetics and pharmaceuticals to prevent or reverse skin aging year by year, designing multiple-molecule drugs for mitigating skin aging is indispensable. In this study, we developed strategies for systems medicine design based on systems biology methods and deep neural networks. We constructed the candidate genomewide genetic and epigenetic network (GWGEN) via big database mining. After doing systems modeling and applying system identification, system order detection and principle network projection methods with real time-profile microarray data, we could obtain core signaling pathways and identify essential biomarkers based on the skin aging molecular progression mechanisms. Afterwards, we trained a deep neural network of drug–target interaction in advance and applied it to predict the potential candidate drugs based on our identified biomarkers. To narrow down the candidate drugs, we designed two filters considering drug regulation ability and drug sensitivity. With the proposed systems medicine design procedure, we not only shed the light on the skin aging molecular progression mechanisms but also suggested two multiple-molecule drugs for mitigating human skin aging from young adulthood to middle age and middle age to old age, respectively.

scholarly journals Exploring Human Skin Aging at the Single-Cell Level

2021 ◽  
Vol 56 (3) ◽  
pp. 253-254
Author(s):  
Mary Mohrin ◽  
Heinrich Jasper
Keyword(s):  
Single Cell ◽  
Human Skin ◽  
Skin Aging ◽  
Single Cell Level ◽  
Cell Level
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