Anti-inflammatory effects of Erythromycin and Tetracycline on Propionibacterium. acnes induced production of chemotactic factors and reactive oxygen species by human neutrophils

2002 ◽  
Vol 8 (2) ◽  
Author(s):  
A Jain ◽  
L Sangal ◽  
E Basal ◽  
P Kaushal G ◽  
SK Agarwal
Keyword(s):  
Reactive Oxygen Species ◽  
Propionibacterium Acnes ◽  
Reactive Oxygen ◽  
Human Neutrophils ◽  
Oxygen Species ◽  
Chemotactic Factors ◽  
Anti Inflammatory

scholarly journals Alteration of Neutrophil Reactive Oxygen Species Production by Extracts of Devil’s Claw (Harpagophytum)

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Mbaki Muzila ◽  
Kimmo Rumpunen ◽  
Helen Wright ◽  
Helen Roberts ◽  
Melissa Grant ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Respiratory Burst ◽  
Dna Analysis ◽  
Reactive Oxygen ◽  
Human Neutrophils ◽  
Putative Hybrid ◽  
Oxygen Species ◽  
New Variety ◽  
Devil’S Claw ◽  
Anti Inflammatory

Harpagophytum, Devil’s Claw, is a genus of tuberiferous xerophytic plants native to southern Africa. Some of the taxa are appreciated for their medicinal effects and have been traditionally used to relieve symptoms of inflammation. The objectives of this pilot study were to investigate the antioxidant capacity and the content of total phenols, verbascoside, isoverbascoside, and selected iridoids, as well as to investigate the capacity of variousHarpagophytumtaxa in suppressing respiratory burst in terms of reactive oxygen species produced by human neutrophils challenged with phorbol myristate acetate (PMA), opsonisedStaphylococcus aureus,andFusobacterium nucleatum.Harpagophytumplants were classified into different taxa according to morphology, and DNA analysis was used to confirm the classification. A putative new variety ofH. procumbensshowed the highest degree of antioxidative capacity. Using PMA, threeHarpagophytumtaxa showed anti-inflammatory effects with regard to the PBS control. A putative hybrid betweenH. procumbensandH. zeyheriin contrast showed proinflammatory effect on the response of neutrophils toF. nucleatumin comparison with treatment with vehicle control.Harpagophytumtaxa were biochemically very variable and the response in suppressing respiratory burst differed. Further studies with larger number of subjects are needed to corroborate anti-inflammatory effects of different taxa ofHarpagophytum.

scholarly journals Simultaneous use of electrochemistry and chemiluminescence to detect reactive oxygen species produced by human neutrophils

2008 ◽  
Vol 32 (12) ◽  
pp. 1486-1496 ◽  
Author(s):  
Sergey Shleev ◽  
Jonas Wetterö ◽  
Karl-Eric Magnusson ◽  
Tautgirdas Ruzgas
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Human Neutrophils ◽  
Oxygen Species ◽  
Simultaneous Use

scholarly journals The Effect of Physical Training on Peripheral Blood Mononuclear Cell Ex Vivo Proliferation, Differentiation, Activity, and Reactive Oxygen Species Production in Racehorses

Antioxidants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1155
Author(s):  
Olga Witkowska-Piłaszewicz ◽  
Rafał Pingwara ◽  
Anna Winnicka
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Proliferation ◽  
Immune Cell ◽  
Ex Vivo ◽  
Exercise Physiology ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Peripheral Blood Mononuclear ◽  
Before And After ◽  
Anti Inflammatory

Physical activity has an influence on a variety of processes in an athlete’s organism including the immune system. Unfortunately, there is a lack of studies regarding racehorse immune cells, especially when the horse model is compared to human exercise physiology. The aim of the study was to determine changes in immune cell proliferation, lymphocyte populations, and monocyte functionality in trained and untrained racehorses after exercise. In this study, field data were collected. The cells from 28 racehorses (14 untrained and 14 well-trained) were collected before and after exercise (800 m at a speed of about 800 m/min) and cultured for 4 days. The expression of CD4, CD8, FoxP3, CD14, MHCII, and CD5 in PBMC, and reactive oxygen species (ROS) production, as well as cell proliferation, were evaluated by flow cytometry. In addition, IL-1β, IL-4, IL-6, IL-10, IL-17, INF-γ, and TNF-α concentrations were evaluated by ELISA. The creation of an anti-inflammatory environment in well-trained horses was confirmed. In contrast, a pro-inflammatory reaction occurred in untrained horses after training. In conclusion, an anti-inflammatory state occurs in well-trained racehorses, which is an adaptational reaction to an increased workload during training.

Involvement of Caspases in Neutrophil Apoptosis: Regulation by Reactive Oxygen Species

Blood ◽  
1998 ◽  
Vol 92 (12) ◽  
pp. 4808-4818 ◽  
Author(s):  
Bengt Fadeel ◽  
Anders Åhlin ◽  
Jan-Inge Henter ◽  
Sten Orrenius ◽  
Mark B. Hampton
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Death ◽  
Nadph Oxidase ◽  
Reactive Oxygen ◽  
Human Neutrophils ◽  
Neutrophil Apoptosis ◽  
Dependent Manner ◽  
Caspase Activity ◽  
Oxygen Species ◽  
Spontaneous Apoptosis

Abstract Human neutrophils have a short half-life and are believed to die by apoptosis or programmed cell death both in vivo and in vitro. We found that caspases are activated in a time-dependent manner in neutrophils undergoing spontaneous apoptosis, concomitant with other characteristic features of apoptotic cell death such as morphologic changes, phosphatidylserine (PS) exposure, and DNA fragmentation. The treatment of neutrophils with agonistic anti-Fas monoclonal antibodies (MoAbs) significantly accelerated this process. However, in cells treated with the potent neutrophil activator phorbol 12-myristate 13-acetate (PMA), caspase activity was only evident after pharmacologic inhibition of the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase. Similarily, inhibition of the NADPH oxidase in constitutive and Fas/APO-1–triggered apoptosis resulted in increased rather than suppressed levels of caspase activity, suggesting that reactive oxygen species may prevent caspases from functioning optimally in these cells. Moreover, oxidants generated via the NADPH oxidase were essential for PS exposure during PMA-induced cell death, but not for neutrophils undergoing spontaneous apoptosis. We conclude that caspases are an important component of constitutive and Fas/APO-1–triggered neutrophil apoptosis. However, these redox sensitive enzymes are suppressed in activated neutrophils, and an alternate oxidant-dependent pathway is used to mediate PS exposure and neutrophil clearance under these conditions.

Reactive oxygen species-induced activation of ERK and p38 MAPK mediates PMA-induced NETs release from human neutrophils

2013 ◽  
Vol 114 (3) ◽  
pp. 532-540 ◽  
Author(s):  
Ravi S. Keshari ◽  
Anupam Verma ◽  
Manoj K. Barthwal ◽  
Madhu Dikshit
Keyword(s):  
Reactive Oxygen Species ◽  
P38 Mapk ◽  
Reactive Oxygen ◽  
Human Neutrophils ◽  
Oxygen Species
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