scholarly journals An Economic Sampling Protocol for Locating Citrus tristeza virus Reservoirs in a Large Area

2002 ◽  
Vol 15 (15) ◽  
Author(s):  
R. K. Yokomi ◽  
M. L. Polek ◽  
S. Satar ◽  
T. R. Gottwald
Keyword(s):  
Citrus Tristeza Virus ◽  
Large Area ◽  
Sampling Protocol ◽  
Tristeza Virus ◽  
Citrus Tristeza

scholarly journals Survey Methods for Assessment of Citrus tristeza virus Incidence in Urban Citrus Populations

Plant Disease ◽  
2002 ◽  
Vol 86 (4) ◽  
pp. 367-372 ◽  
Author(s):  
G. Hughes ◽  
T. R. Gottwald ◽  
K. Yamamura
Keyword(s):  
Survey Methodology ◽  
Citrus Tristeza Virus ◽  
Survey Methods ◽  
Acceptance Sampling ◽  
Large Area ◽  
Two Stage ◽  
Commercial Cultivation ◽  
Virus Incidence ◽  
Tristeza Virus ◽  
Citrus Tristeza

This article concerns survey methodology for pathogens in urban citrus populations, motivated in particular by the need for assessments of Citrus tristeza virus (CTV) incidence. We envisage a large area R not devoted primarily to the commercial cultivation of citrus, that nevertheless has a substantial population of citrus trees. It is desired to sample the citrus population of area R in order to be able to make a statement about the level of infection of the population with CTV, or with particular isolates thereof. We describe a two-stage acceptance sampling scheme in which area R is divided into N sampling units, of which n are inspected. The size of the sampling units, while much smaller than R, is still large, so subsampling is carried out, introducing the possibility of misclassification of sampling units. To account for misclassification of sampling units, a larger number must be inspected than if it were assumed that there were no misclassifications. We describe the calculation of sample sizes required for subsampling within sampling units and for the total number of sampling units to be inspected, using parameters that can be adjusted to meet different specified regulatory scenarios.

First report of citrus tristeza virus in commercial citrus orchards in Tunisia

2021 ◽  
Author(s):  
Asma Najar ◽  
Imen Hamdi ◽  
Souad Mahmoud ◽  
Lassaad Medhioub ◽  
Imed Jaouadi ◽  
...  
Keyword(s):  
Citrus Tristeza Virus ◽  
First Report ◽  
Citrus Orchards ◽  
Tristeza Virus ◽  
Citrus Tristeza

A model for the spread and control of citrus tristeza virus disease

1989 ◽  
Vol 16 (3) ◽  
pp. 315-320
Author(s):  
Ruth Marcus ◽  
Hovav Talpaz ◽  
Moshe Bar-Joseph
Keyword(s):  
Citrus Tristeza Virus ◽  
Virus Disease ◽  
And Control ◽  
Tristeza Virus ◽  
Citrus Tristeza

Sscp analysis of variations in haplotypes of citrus tristeza virus isolated from yuzu (Citrus junos) in geographically separate regions of Korea

2006 ◽  
Vol 49 (1) ◽  
pp. 88-96 ◽  
Author(s):  
Dae Hyun Kim ◽  
Hye Kyung Shim ◽  
Jae Wook Hyeon ◽  
Hyeog Mo Kwon ◽  
Kwang Sik Kim ◽  
...  
Keyword(s):  
Citrus Tristeza Virus ◽  
Sscp Analysis ◽  
Citrus Junos ◽  
Tristeza Virus ◽  
Citrus Tristeza

Production of Antibodies to Citrus Tristeza Virus in Transgenic Citrus

1995 ◽  
Author(s):  
Richard Lee ◽  
Moshe Bar-Joseph ◽  
K.S. Derrick ◽  
Aliza Vardi ◽  
Roland Brlansky ◽  
...  
Keyword(s):  
Citrus Tristeza Virus ◽  
Virus Disease ◽  
Biological Activities ◽  
Cp Gene ◽  
Single Domain Antibodies ◽  
Tristeza Virus ◽  
Stem Pitting ◽  
Citrus Tristeza

Citrus tristeza virus (CTV) is the most important virus disease of citrus in the world. CTV causes death of trees on sour orange rootstock and/or stem pitting of scions regardless of rootstock which results in trees of low vigor, reduced yield with reduction in size and quality of fruit. The purpose of this project was to produce monoclonal antibodies (MABs) to CTV coat protein (CP), develop single domain antibodies (dAbs) or Fab fragments which neutralize the infection by binding to the virus, and to produce transformed plants which express the dAbs. The objectives of this research have been met and putative transgenic tobacco and citrus plants have been developed. These putative transgenic plants are presently undergoing evaluation to determine the level of dAbs expression and to determine their resistance to CTV. Additionally, the CTV genome has been sequenced and the CP gene of several biologically characterized CTV strains molecular characterized. This has indicated a correlation between CP sequence homology and biological activity, and the finding of DI RNAs associated with some CTV strains. Several MABs have been produced which enable broad spectrum identification of CTV strains while other MABs enable differentiation between mild and severe strains. The use of selected MAbs and determination of the CP gene sequence has enabled predictions of biological activities of unknown CTV isolates. The epitopes of two MABs, one reacting selectively with severe CTV strains and the other reacting with all strains, have been characterized at the molecular level.

scholarly journals Evaluation of the protective capacity of new mild Citrus tristeza virus (CTV) isolates selected for a preimmunization program

2013 ◽  
Vol 70 (2) ◽  
pp. 116-124 ◽  
Author(s):  
Carlos Alexandre Zanutto ◽  
Maria Júlia Corazza ◽  
William Mário de Carvalho Nunes ◽  
Gerd Walter Müller
Keyword(s):  
Citrus Tristeza Virus ◽  
Protective Capacity ◽  
Tristeza Virus ◽  
Citrus Tristeza

scholarly journals Eliminação de vírus do complexo sorose dos citros por microenxertia associada a termoterapia

2002 ◽  
Vol 27 (3) ◽  
pp. 306-308 ◽  
Author(s):  
SÉRGIO A. CARVALHO ◽  
FRANCISCA A. SANTOS ◽  
MARCOS A. MACHADO
Keyword(s):  
Citrus Tristeza Virus ◽  
Citrus Exocortis Viroid ◽  
Tristeza Virus ◽  
Citrus Tristeza

A microenxertia de ápices caulinares tem sido utilizada com 100% de sucesso na eliminação do vírus da tristeza (Citrus tristeza virus) e dos viróides da exocorte (Citrus exocortis viroid - CEVd) e cachexia-xiloporose de materiais do Banco Ativo de Germoplasma de Citros do Centro de Citricultura Sylvio Moreira CCSM-IAC. Para o complexo da sorose, entretanto, esta técnica tem apresentado somente 60% de eficiência, indicando a necessidade de sua associação com termoterapia para garantir a eliminação viral. Para tanto, mudas originadas de borbulhas infetadas com sorose foram mantidas em câmara climática com 16 h de luz a 38 ºC e 8 h no escuro a 32 ºC e utilizadas para a obtenção dos ápices caulinares empregados na microenxertia. Após o pegamento, o conjunto micro porta-enxerto e brotação foi sobre-enxertado em limoeiro (Citrus limonia) 'Cravo' com sete meses de idade e mantido em condições de casa de vegetação. Clones de laranjeiras doces (Citrus sinsensis) 'Lima', 'Rubi', 'Piralima', 'Salustiana', 'João Nunes', 'Rosa' e 'Pêra Caire' tratados desta maneira, comprovaram eficiência de 100% de eliminação do complexo sorose, conforme indexação realizada empregando-se laranjeira 'Do Céu' como planta indicadora.

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