scholarly journals Characterization of Citrus Tristeza Virus Isolates by Single-Strand Conformation Polymorphism Analysis of DNA Complementary to Their RNA Population

2000 ◽  
Vol 14 (14) ◽  
Author(s):  
L. Rubio ◽  
J. Guerri ◽  
P. Moreno
Keyword(s):  
Citrus Tristeza Virus ◽  
Single Strand Conformation Polymorphism ◽  
Single Strand ◽  
Polymorphism Analysis ◽  
Conformation Polymorphism Analysis ◽  
Tristeza Virus ◽  
Virus Isolates ◽  
Conformation Polymorphism ◽  
Citrus Tristeza

scholarly journals Application of biological and single-strand conformation polymorphism assays for characterizing potential mild isolates of Citrus tristeza virus for cross protection

AMB Express ◽  
2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Sagheer Atta ◽  
Ummad ud din Umar ◽  
Muhammad Amjad Bashir ◽  
Abdul Hannan ◽  
Ateeq ur Rehman ◽  
...  
Keyword(s):  
Citrus Tristeza Virus ◽  
Single Strand Conformation Polymorphism ◽  
Biological Indicator ◽  
Cross Protection ◽  
Single Strand ◽  
Sequence Variant ◽  
Viral Pathogen ◽  
Tristeza Virus ◽  
Conformation Polymorphism ◽  
Citrus Tristeza

Abstract Citrus tristeza virus (CTV) by killing millions of citrus cultivars grown on sour orange rootstock worldwide has become one of the most dangerous viral pathogen. Characterization of 12 CTV isolates was analyzed by biological indexing. Infected samples of citrus were collected from citrus growing areas of Pakistan and CTV was detected by symptoms on indicator plants and confirmed by direct tissue blot immunoassay (DTBIA). CTV positive samples were graft inoculated on six biological indicator hosts in the study. A standardized protocol was deployed to study biological characteristics of these isolates. All biological indicators induced mild and from mild to moderate reactions against all of the CTV isolates tested. About two isolates produced stem-pitting symptoms from moderate to severe on Mexican lime. CTV strains were further characterized and confirmed by the analysis of p25 gene of CTV isolates using single-strand conformation polymorphism (SSCP) assay. SSCP analysis revealed that most isolates confined only one predominant sequence variant. SSCP profiles of PCR amplified products from CTV isolates showed bands patterns corresponding to mild and sever strain. Three isolates (4MF, 8KBS and 10GS) from different regions and cultivars were identified as potential source of mild strains for cross protection. These results are the best base for mild strain cross protection (MSCP) in the country.

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