scholarly journals An Improved Protocol for Extraction and RT-PCR Detection of Citrus Viroids

2005 ◽  
Vol 16 (16) ◽  
Author(s):  
L. Bernad ◽  
N. Duran-Vila
Keyword(s):  
Pcr Detection ◽  
Rt Pcr ◽  
Citrus Viroids

Simultaneous multiplex RT-PCR detection of four viruses associated with maize lethal necrosis disease

2021 ◽  
pp. 114286
Author(s):  
Xiaoqin Li ◽  
Yu Li ◽  
Wenli Hu ◽  
Yingjuan Li ◽  
Yan Li ◽  
...  
Keyword(s):  
Pcr Detection ◽  
Rt Pcr

Multiplex RT-PCR detection and microarray typing of vesicular disease viruses

2011 ◽  
Vol 175 (2) ◽  
pp. 236-245 ◽  
Author(s):  
Oliver Lung ◽  
Mathew Fisher ◽  
Anne Beeston ◽  
Kimberley Burton Hughes ◽  
Alfonso Clavijo ◽  
...  
Keyword(s):  
Pcr Detection ◽  
Rt Pcr ◽  
Vesicular Disease

scholarly journals RT-PCR Detection of Five Quarantine Plant RNA Viruses Belonging to Potyand Tospoviruses

2011 ◽  
Vol 27 (3) ◽  
pp. 291-296 ◽  
Author(s):  
Jong-Seung Lee ◽  
Won-Kyong Cho ◽  
Hong-Soo Choi ◽  
Kook-Hyung Kim
Keyword(s):  
Rna Viruses ◽  
Pcr Detection ◽  
Rt Pcr

scholarly journals Practical Field Detection of Citrus Viroids in Florida by RT-PCR

2002 ◽  
Vol 15 (15) ◽  
Author(s):  
S. M. Garnsey ◽  
D. L. Zies ◽  
M. Irey ◽  
P. J. Sieburth ◽  
JS S. Semancik ◽  
...  
Keyword(s):  
Rt Pcr ◽  
Field Detection ◽  
Citrus Viroids ◽  
Practical Field

scholarly journals Potential Use of Serum Proteomics for Monitoring COVID-19 Progression to Complement RT-PCR Detection

2021 ◽  
Author(s):  
Ying Zhang ◽  
Xue Cai ◽  
Weigang Ge ◽  
Donglian Wang ◽  
Guangjun Zhu ◽  
...  
Keyword(s):  
Pcr Detection ◽  
Rt Pcr ◽  
Serum Proteomics ◽  
Potential Use

scholarly journals Detection of Multiple Infections of Citrus exocortis viroid, Citrus viroid III, and Hop stunt viroid Variants in Hunan Province, China

Plant Disease ◽  
2007 ◽  
Vol 91 (9) ◽  
pp. 1205-1205 ◽  
Author(s):  
S. Rizza ◽  
A. Catara ◽  
X. F. Ma ◽  
Z. Deng
Keyword(s):  
Polyacrylamide Gel Electrophoresis ◽  
Leaf Roll ◽  
Poncirus Trifoliata ◽  
Hunan Province ◽  
Variable Degree ◽  
Rt Pcr ◽  
Stunt Viroid ◽  
Citrus Exocortis Viroid ◽  
Hop Stunt Viroid ◽  
Citrus Viroids

Citrus cultivation in China has increased since the late 1970s, with China now having the largest area of citrus in culture in the world that is spread in 22 provinces and municipalities. Hunan Province has undergone a program to become one of the major citrus producers in China. Poncirus trifoliata is the main rootstock, so citrus viroids are a limiting factor for further citriculture development. In mainland China, only the presence of Citrus exocortis viroid (CEVd) has been reported from Etrog citron indexing, sPAGE (sequential polyacrylamide gel electrophoresis) analysis (2), and reverse transcription (RT)-PCR (3). Three viroid-like RNAs, a1, b1, and d, based on sPAGE patterns were detected years ago in our laboratory in budsticks received from Sichuan Province. To identify different viroids and determine their distribution, a survey has been undertaken. Field trees showing stunting, bark scaling and cracking of the rootstock, and poor yield were tested using biological indexing and PCR for the most frequent citrus viroids. Samples from six trees of a local sweet orange variety and three of a Clementine variety introduced from abroad, both grafted on P. trifoliata and showing a variable degree of bark scaling and cracking, were collected near Changsha and in the County of Xin Ning at the end of summer 2006. Small pieces of bark were inserted in stems of young E. citron budwood grafted on rough lemon and maintained in a warm greenhouse (24 to 32°C). Indexing on E. citron showed mild epinasty and leaf roll typical of citrus viroid infections. To identify specific viroids, bark was ground to a fine powder with liquid nitrogen and total RNA was extracted with TRIZOL Reagent (Invitrogen, San Diego, CA) and tested by RT-PCR to detect CEVd, Hop Stunt viroid (HSVd), and Citrus viroid III (CVd-III), as well as to identify the cachexia variants of HSVd. Four primer pairs were used to test the RNA extracts by RT-PCR (1). All samples were infected by HSVd, eight with CVd-III, and six with CEVd. The cachexia variants of HSVd were detected in four of nine samples. Mixed infections were as follows: one sample had CEVd and HSVd, eight had HSVd and CVd-III, and five were infected by the three viroids. A second sampling 3 months after inoculation gave the same amplification patterns. The results show that at least three viroids are present in citrus orchards in Hunan Province. To our knowledge, this is the first report of cachexia variants of HSVd and CVd-III in China. The common occurrence of these viroids supports the need for proper indexing of mother trees and a specific shoot tip grafting program to create healthy budwood sources to provide healthy plants. References: (1) L. Bernard and N. Duran-Vila. Mol. Cell. Probes, 20:105, 2006. (2) L. Han et al. Viroids. CSIRO Publishing, Melbourne, 283, 2003. (3). Q. Hu et al. Acta Bot. Sin. 39:613, 1997.

scholarly journals RT-PCR Detection of RNA Viruses in Stool Specimens

BioTechniques ◽  
1997 ◽  
Vol 23 (4) ◽  
pp. 616-618 ◽  
Author(s):  
Michael A. Drebot ◽  
Spencer H.S. Lee
Keyword(s):  
Rna Viruses ◽  
Pcr Detection ◽  
Rt Pcr ◽  
Stool Specimens
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