scholarly journals Molecular Interactions between the Citrus Bacterial Pathogen Candidatus Liberbacter asiaticus and Its Insect Vector the Asian Citrus Psyllid Diaphorina citri

2014 ◽  
Vol 1 (1) ◽  
Author(s):  
Linling Wang ◽  
Nabil Killiny
Keyword(s):  
Molecular Interactions ◽  
Bacterial Pathogen ◽  
Asian Citrus Psyllid ◽  
Insect Vector ◽  
Diaphorina Citri

scholarly journals Evaluation of the efficacy of neonicotinoid and pyrethroid insecticides in Diaphorina citri Kuwayama (Hemiptera: Liviidae) populations in Colombia

2018 ◽  
Vol 12 (2) ◽  
pp. 358-368
Author(s):  
Augusto Ramírez-Godoy ◽  
Gina Puentes-Pérez ◽  
Hermann Restrepo-Díaz
Keyword(s):  
Tropical Dry Forest ◽  
Dry Forest ◽  
Asian Citrus Psyllid ◽  
Insect Vector ◽  
Diaphorina Citri ◽  
Pyrethroid Insecticides ◽  
Lambda Cyhalothrin ◽  
The Mean ◽  
Number Of Individuals ◽  
Forest Conditions

The Asian citrus psyllid Diaphorina citri is the insect vector of the disease known as huanglongbing (HLB), which is the most devastating disease of citrus crops in the world. The Asian citrus psyllid was officially reported in Colombia in 2007, and a national phytosanitary emergency was declared because of the presence of HLB in 2015. Two different experiments were carried out in two locations (Apulo and Jerusalén, Department of Cundinamarca) in Colombia to evaluate the effectiveness of neonicotinoid (clothianidin, imidacloprid and thiamethoxan) and pyrethroid applications (bifenthrin, deltamethrin and lambda cyhalothrin) on the control of D. citri populations (adults, nymphs and eggs) in ‘Tahiti’ lime trees established under tropical dry forest conditions. In the first experiment, trees were foliarly treated as follows: (1) untreated trees; (2) trees treated with 100 g ha-1 of imidacloprid; (3) trees treated with 60 g ha-1 of bifenthrin; (4) trees treated with 12.5 g ha-1 of deltamethrin and (5) trees treated with a co-formulation of 70 g ha-1 of a neonicotinoid (thiametoxan) and 53 g ha-1 of a pyrethroid (lambda cyhalothrin). In the second experiment, trees were treated with 100 g ha-1 of clothianidin. Foliar insecticide applications were carried out at 0 and 4 weeks after the initiation of treatments (WAT). In the first trial, the control presented a mean number of individuals per flush of ≈1.14, whereas the trees treated with neoinicotinoids and pyrethroids showed 50% fewer individuals (0.53-0.61). For the population of immatures, the number of nymphs was 2.25 nymphs per flush in the control, as compared to 0.82-1.22 individuals observed in the treatments with insecticides. The mean number of eggs was also between 80-100% higher in the control trees (2.37 individuals), as compared to the treated trees (1.14-1.78). In the second trial, the use of clothianidin showed a higher control of eggs. The results suggest that the rotation of neonicotinoids with pyrethroids can be a tool to reduce populations and delay resistance processes in D. citri in citrus trees established under tropical dry forest conditions in Colombia.

scholarly journals Metabolic Profiling of Hybrids Generated from Pummelo and Citrus latipes in Relation to Their Attraction to Diaphorina citri, the Vector of Huanglongbing

Metabolites ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 477
Author(s):  
Nabil Killiny ◽  
Shelley E. Jones ◽  
Faraj Hijaz ◽  
Abdelaziz Kishk ◽  
Yulica Santos-Ortega ◽  
...  
Keyword(s):  
Leaf Morphology ◽  
Bacterial Pathogen ◽  
Gas Chromatography Mass Spectrometry ◽  
Insect Vector ◽  
Diaphorina Citri ◽  
Metabolomic Analysis ◽  
Citrus Industry ◽  
Candidatus Liberibacter ◽  
Citrus Maxima ◽  
Liberibacter Asiaticus

The citrus industry at present is severely affected by huanglongbing disease (HLB). HLB is caused by the supposed bacterial pathogen “Candidatus Liberibacter asiaticus” and is transmitted by the insect vector, the Asian citrus psyllid, Diaphorina citri Kuwayama. Developing new citrus hybrids to improve HLB management is much needed. In this study, we investigated the metabolomic profiles of three new hybrids produced from the cross of C2-5-12 Pummelo (Citrus maxima (L.) Osbeck) × pollen from Citrus latipes. The hybrids were selected based on leaf morphology and seedling vigor. The selected hybrids exhibited compact and upright tree architecture as seen in C. latipes. Hybrids were verified by simple sequence repeat markers, and were subjected to metabolomic analysis using gas chromatography-mass spectrometry. The volatile organic compounds (VOCs) and polar metabolites profiling also showed that the new hybrids were different from their parents. Interestingly, the levels of stored VOCs in hybrid II were higher than those observed in its parents and other hybrids. The level of most VOCs released by hybrid II was also higher than that released from its parents. Additionally, the preference assay showed that hybrid II was more attractive to D. citri than its parents and other hybrids. The leaf morphology, compact and upright architecture of hybrid II, and its attraction to D. citri suggest that it could be used as a windbreak and trap tree for D. citri (double duty), once its tolerance to HLB disease is confirmed. Our results showed that metabolomic analysis could be successfully used to understand the biochemical mechanisms controlling the interaction of D. citri with its host plants.

scholarly journals Protein interaction networks at the host–microbe interface in Diaphorina citri , the insect vector of the citrus greening pathogen

2017 ◽  
Vol 4 (2) ◽  
pp. 160545 ◽  
Author(s):  
J. S. Ramsey ◽  
J. D. Chavez ◽  
R. Johnson ◽  
S. Hosseinzadeh ◽  
J. E. Mahoney ◽  
...  
Keyword(s):  
Protein Interaction ◽  
Bacterial Pathogen ◽  
Pathogen Transmission ◽  
Protein Interaction Networks ◽  
Interaction Networks ◽  
Differentially Expressed ◽  
Citrus Greening ◽  
Insect Vector ◽  
Diaphorina Citri ◽  
Citrus Greening Disease

The Asian citrus psyllid ( Diaphorina citri) is the insect vector responsible for the worldwide spread of ‘ Candidatus Liberibacter asiaticus’ (CLas), the bacterial pathogen associated with citrus greening disease. Developmental changes in the insect vector impact pathogen transmission, such that D. citri transmission of CLas is more efficient when bacteria are acquired by nymphs when compared with adults. We hypothesize that expression changes in the D. citri immune system and commensal microbiota occur during development and regulate vector competency. In support of this hypothesis, more proteins, with greater fold changes, were differentially expressed in response to CLas in adults when compared with nymphs, including insect proteins involved in bacterial adhesion and immunity. Compared with nymphs, adult insects had a higher titre of CLas and the bacterial endosymbionts Wolbachia, Profftella and Carsonella. All Wolbachia and Profftella proteins differentially expressed between nymphs and adults are upregulated in adults, while most differentially expressed Carsonella proteins are upregulated in nymphs. Discovery of protein interaction networks has broad applicability to the study of host–microbe relationships. Using protein interaction reporter technology, a D. citri haemocyanin protein highly upregulated in response to CLas was found to physically interact with the CLas coenzyme A (CoA) biosynthesis enzyme phosphopantothenoylcysteine synthetase/decarboxylase. CLas pantothenate kinase, which catalyses the rate-limiting step of CoA biosynthesis, was found to interact with a D. citri myosin protein. Two Carsonella enzymes involved in histidine and tryptophan biosynthesis were found to physically interact with D. citri proteins. These co-evolved protein interaction networks at the host–microbe interface are highly specific targets for controlling the insect vector responsible for the spread of citrus greening.

scholarly journals Characterization of Wnt Signaling Genes in Diaphorina citri, Asian citrus psyllid

2020 ◽  
Author(s):  
Chad Vosburg ◽  
Max Reynolds ◽  
Rita Noel ◽  
Teresa Shippy ◽  
Prashant S Hosmani ◽  
...  
Keyword(s):  
Wnt Signaling ◽  
Signaling Pathways ◽  
Asian Citrus Psyllid ◽  
Insect Vector ◽  
Beta Catenin ◽  
Manual Annotation ◽  
Diaphorina Citri ◽  
Sequencing Data ◽  
Wnt Ligands ◽  
Gene Models

The Asian citrus psyllid, Diaphorina citri, is an insect vector that transmits Candidatus Liberibacter asiaticus, the causal agent of the Huanglongbing (HLB) or citrus greening disease. This disease has devastated Florida's citrus industry and threatens California's industry as well as other citrus producing regions around the world. To find novel solutions to the disease, a better understanding of the vector is needed. The D. citri genome has been used to identify and characterize genes involved in Wnt signaling pathways. Wnt signaling is utilized for many important biological processes in metazoans, such as patterning and tissue generation. Curation based on RNA sequencing data and sequence homology confirm twenty four Wnt signaling genes within the D. citri genome, including homologs for beta-catenin, Frizzled receptors, and seven Wnt-ligands. Through phylogenetic analysis, we classify D. citri Wnt-ligands as Wg/Wnt1, Wnt5, Wnt6, Wnt7, Wnt10, Wnt11, and WntA. The D. citri version 3.0 genome with chromosomal length scaffolds reveals a conserved Wnt1-Wnt6-Wnt10 gene cluster with gene configuration similar to that in Drosophila melanogaster. These findings provide a greater insight into the evolutionary history of D. citri and Wnt signaling in this important hemipteran vector. Manual annotation was essential for identifying high quality gene models. These gene models can further be used to develop molecular systems, such as CRISPR and RNAi, that target and control D. citri populations, to manage the spread of HLB. Manual annotation of Wnt signaling pathways was done as part of a collaborative community annotation project (https://citrusgreening.org/annotation/index).

scholarly journals Diverse Array of New Viral Sequences Identified in Worldwide Populations of the Asian Citrus Psyllid (Diaphorina citri) Using Viral Metagenomics

2015 ◽  
Vol 90 (5) ◽  
pp. 2434-2445 ◽  
Author(s):  
Shahideh Nouri ◽  
Nidá Salem ◽  
Jared C. Nigg ◽  
Bryce W. Falk
Keyword(s):  
Bioinformatics Analysis ◽  
Rna Virus ◽  
Insect Pest ◽  
Viral Metagenomics ◽  
Asian Citrus Psyllid ◽  
Insect Vector ◽  
Diaphorina Citri ◽  
Related Sequence ◽  
Content Type ◽  
Viral Sequences

ABSTRACTThe Asian citrus psyllid,Diaphorina citri, is the natural vector of the causal agent of Huanglongbing (HLB), or citrus greening disease. Together; HLB andD. citrirepresent a major threat to world citrus production. As there is no cure for HLB, insect vector management is considered one strategy to help control the disease, andD. citriviruses might be useful. In this study, we used a metagenomic approach to analyze viral sequences associated with the global population ofD. citri. By sequencing small RNAs and the transcriptome coupled with bioinformatics analysis, we showed that the virus-like sequences ofD. citriare diverse. We identified novel viral sequences belonging to the picornavirus superfamily, theReoviridae,Parvoviridae, andBunyaviridaefamilies, and an unclassified positive-sense single-stranded RNA virus. Moreover, aWolbachiaprophage-related sequence was identified. This is the first comprehensive survey to assess the viral community from worldwide populations of an agricultural insect pest. Our results provide valuable information on new putative viruses, some of which may have the potential to be used as biocontrol agents.IMPORTANCEInsects have the most species of all animals, and are hosts to, and vectors of, a great variety of known and unknown viruses. Some of these most likely have the potential to be important fundamental and/or practical resources. In this study, we used high-throughput next-generation sequencing (NGS) technology and bioinformatics analysis to identify putative viruses associated withDiaphorina citri, the Asian citrus psyllid.D. citriis the vector of the bacterium causing Huanglongbing (HLB), currently the most serious threat to citrus worldwide. Here, we report several novel viral sequences associated withD. citri.

scholarly journals Crystal structure of diaphorin methanol monosolvate isolated from Diaphorina citri Kuwayama, the insect vector of citrus greening disease

2018 ◽  
Vol 74 (4) ◽  
pp. 445-449 ◽  
Author(s):  
D. Marian Szebenyi ◽  
Irina Kriksunov ◽  
Kevin J. Howe ◽  
John S. Ramsey ◽  
David G. Hall ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Title Compound ◽  
Asian Citrus Psyllid ◽  
Insect Vector ◽  
Diaphorina Citri ◽  
Relative Configuration ◽  
Citrus Greening Disease ◽  
Compound C ◽  
Curr Biol ◽  
Greening Disease

The title compound C22H39NO9·CH3OH [systematic name: (S)-N-((S)-{(2S,4R,6R)-6-[(S)-2,3-dihydroxypropyl]-4-hydroxy-5,5-dimethyltetrahydro-2H-pyran-2-yl}(hydroxy)methyl)-2-hydroxy-2-[(2R,5R,6R)-2-methoxy-5,6-dimethyl-4-methylenetetrahydro-2H-pyran-2-yl]acetamide methanol monosolvate], was isolated from the Asian citrus psyllid, Diaphorina citri Kuwayama, and crystallizes in the space group P21. `Candidatus Profftella armatura' a bacterial endosymbiont of D. citri, biosynthesizes diaphorin, which is a hybrid polyketide–nonribosomal peptide comprising two highly substituted tetrahydropyran rings joined by an N-acyl aminal bridge [Nakabachi et al. (2013). Curr. Biol. 23, 1478–1484]. The crystal structure of the title compound establishes the complete relative configuration of diaphorin, which agrees at all nine chiral centers with the structure of the methanol monosolvate of the di-p-bromobenzoate derivative of pederin, a biogenically related compound whose crystal structure was reported previously [Furusaki et al. (1968). Tetrahedron Lett. 9, 6301–6304]. Thus, the absolute configuration of diaphorin is proposed by analogy to that of pederin.

scholarly journals Utilizing a chromosomal-length genome assembly to annotate the Wnt signaling pathway in the Asian citrus psyllid, Diaphorina citri

Gigabyte ◽  
2021 ◽  
Vol 2021 ◽  
pp. 1-15
Author(s):  
Chad Vosburg ◽  
Max Reynolds ◽  
Rita Noel ◽  
Teresa Shippy ◽  
Prashant S. Hosmani ◽  
...  
Keyword(s):  
Wnt Signaling ◽  
Signaling Pathways ◽  
Asian Citrus Psyllid ◽  
Insect Vector ◽  
Beta Catenin ◽  
Manual Annotation ◽  
Diaphorina Citri ◽  
Wnt Ligands ◽  
Gene Models ◽  
Chromosomal Length

The Asian citrus psyllid, Diaphorina citri, is an insect vector that transmits Candidatus Liberibacter asiaticus, the causal agent of the Huanglongbing (HLB), or citrus greening disease. This disease has devastated Florida’s citrus industry, and threatens California’s industry as well as other citrus producing regions around the world. To find novel solutions to the disease, a better understanding of the vector is needed. The D. citri genome has been used to identify and characterize genes involved in Wnt signaling pathways. Wnt signaling is utilized for many important biological processes in metazoans, such as patterning and tissue generation. Curation based on RNA sequencing data and sequence homology confirms 24 Wnt signaling genes within the D. citri genome, including homologs for beta-catenin, Frizzled receptors, and seven Wnt-ligands. Through phylogenetic analysis, we classify D. citri Wnt ligands as Wg/Wnt1, Wnt5, Wnt6, Wnt7, Wnt10, Wnt11, and WntA. The D. citri version 3.0 genome with chromosomal length scaffolds reveals a conserved Wnt1-Wnt6-Wnt10 gene cluster with a gene configuration like that in Drosophila melanogaster. These findings provide greater insight into the evolutionary history of D. citri and Wnt signaling in this important hemipteran vector. Manual annotation was essential for identifying high quality gene models. These gene models can be used to develop molecular systems, such as CRISPR and RNAi, which target and control psyllid populations to manage the spread of HLB. Manual annotation of Wnt signaling pathways was done as part of a collaborative community annotation project.

scholarly journals Transcriptomic and Metabolomic Analyses of Diaphorina citri Kuwayama Infected and Non-infected With Candidatus Liberibacter Asiaticus

2021 ◽  
Vol 11 ◽  
Author(s):  
Kai Liu ◽  
Jiawei He ◽  
Ziying Guan ◽  
Mingzhao Zhong ◽  
Rui Pang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Host Plants ◽  
Integrated Analysis ◽  
Asian Citrus Psyllid ◽  
Insect Vector ◽  
Diaphorina Citri ◽  
Liquid Chromatography Mass Spectrometry ◽  
Candidatus Liberibacter Asiaticus ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus

The Asian citrus psyllid Diaphorina citri is the transmission vector of Huanglongbing (HLB), a devastating disease of citrus plants. The bacterium “Candidatus Liberibacter asiaticus” (CLas) associated with HLB is transmitted between host plants by D. citri in a circulative manner. Understanding the interaction between CLas and its insect vector is key for protecting citrus cultivation from HLB damage. Here, we used RNA sequencing and liquid chromatography-mass spectrometry (LC-MS) to analyze the transcriptome and metabolome of D. citri interacting with CLas. We identified 662 upregulated and 532 downregulated genes in CLas-infected insects. These genes were enriched in pathways involving carbohydrate metabolism, the insects’ immune system, and metabolism of cofactors and vitamins. We also detected 105 differential metabolites between CLas-infected and non-infected insects, including multiple nucleosides and lipid-related molecules. The integrated analysis revealed nine pathways—including those of the glycine, serine, threonine, and purine metabolism—affected by the differentially expressed genes from both groups. The network for these pathways was subsequently constructed. Our results thus provide insights regarding the cross-talk between the transcriptomic and metabolomic changes in D. citri in response to CLas infection, as well as information on the pathways and genes/metabolites related to the CLas–D. citri interaction.

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