scholarly journals Detection of Candidatus Liberibacter asiaticus in Diaphorina citri caught on yellow sticky traps during the winter and summer of Sao Paulo State Brazil

2014 ◽  
Vol 1 (1) ◽  
Author(s):  
I. Sala ◽  
E. C. Martins ◽  
D. A.B. Coletti ◽  
L. H. Montesino ◽  
R. B. Bassanezi ◽  
...  
Keyword(s):  
Sao Paulo ◽  
São Paulo ◽  
Diaphorina Citri ◽  
Candidatus Liberibacter Asiaticus ◽  
Sticky Traps ◽  
Paulo State ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus ◽  
São Paulo State ◽  
Yellow Sticky Traps

scholarly journals Liberibacters Associated with Citrus Huanglongbing in Brazil: ‘Candidatus Liberibacter asiaticus’ Is Heat Tolerant, ‘Ca. L. americanus’ Is Heat Sensitive

Plant Disease ◽  
2009 ◽  
Vol 93 (3) ◽  
pp. 257-262 ◽  
Author(s):  
S. A. Lopes ◽  
G. F. Frare ◽  
E. Bertolini ◽  
M. Cambra ◽  
N. G. Fernandes ◽  
...  
Keyword(s):  
Sweet Orange ◽  
Sao Paulo ◽  
São Paulo ◽  
Candidatus Liberibacter Asiaticus ◽  
Paulo State ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus ◽  
São Paulo State ◽  
Heat Tolerant ◽  
Orange Trees

In São Paulo State, Brazil, ‘Candidatus Liberibacter americanus’ and ‘Candidatus Liberibacter asiaticus’ are associated with huanglongbing (HLB). Affected municipalities occur mainly in the central and southern regions, where the annual number of hours above 30°C is two to five times lower than that in the extreme northern and western regions. The influence of temperature on sweet orange trees infected with ‘Ca. L. asiaticus’ or ‘Ca. L. americanus’ was studied in temperature-controlled growth chambers. Symptom progression on new shoots of naturally infected and experimentally graft-inoculated symptomatic sweet orange trees was assessed. Mottled leaves developed on all infected trees at 22 to 24°C, but not on any ‘Ca. L. americanus’–infected trees at 27 to 32°C. Quantitative, real time-PCR was used to determine the liberibacter titers in the trees. After 90 days, ‘Ca. L. asiaticus’–infected trees had high titers at 32 and 35°C, but not at 38°C, while ‘Ca. L. americanus’–infected trees had high titers at 24°C, but at 32°C the titers were very low or the liberibacters could not be detected. Thus, the multiplication of ‘Ca. L. asiaticus’ is not yet affected at 35°C, while a temperature of 32°C is detrimental to ‘Ca. L. americanus’. Thus, ‘Ca. L. americanus’ is less heat tolerant than ‘Ca. L. asiaticus’. The uneven distribution of these two liberibacters in São Paulo State might be in relation with these results.

Factors associated with Diaphorina citri immigration into commercial citrus orchards in São Paulo State, Brazil

2020 ◽  
Author(s):  
Fernando P. F. Zorzenon ◽  
Arthur F. Tomaseto ◽  
Matthew P. Daugherty ◽  
João R. S. Lopes ◽  
Marcelo P. Miranda
Keyword(s):  
Sao Paulo ◽  
São Paulo ◽  
Diaphorina Citri ◽  
Factors Associated ◽  
Paulo State ◽  
São Paulo State ◽  
Citrus Orchards

Citrus huanglongbing in São Paulo State, Brazil: PCR detection of the ‘Candidatus’ Liberibacter species associated with the disease

2005 ◽  
Vol 19 (3) ◽  
pp. 173-179 ◽  
Author(s):  
Diva do Carmo Teixeira ◽  
Jean Luc Danet ◽  
Sandrine Eveillard ◽  
Elaine Cristina Martins ◽  
Waldir Cintra de Jesus Junior ◽  
...  
Keyword(s):  
Sao Paulo ◽  
Pcr Detection ◽  
São Paulo ◽  
Paulo State ◽  
Candidatus Liberibacter ◽  
Citrus Huanglongbing ◽  
São Paulo State

scholarly journals Prevalence of Candidatus Liberibacter spp. in HLB-diseased Citrus Plants in São Paulo State, Brazil

2010 ◽  
Vol 17 (17) ◽  
Author(s):  
H. D. Coletta-Filho ◽  
E. F. Carlos ◽  
L. L. Lotto ◽  
F. C. Luciane ◽  
K. C. S. Alves ◽  
...  
Keyword(s):  
Sao Paulo ◽  
São Paulo ◽  
Paulo State ◽  
Candidatus Liberibacter ◽  
São Paulo State

scholarly journals First Report of the Causal Agent of Huanglongbing (“Candidatus Liberibacter asiaticus”) in Brazil

Plant Disease ◽  
2004 ◽  
Vol 88 (12) ◽  
pp. 1382-1382 ◽  
Author(s):  
H. D. Coletta-Filho ◽  
M. L. P. N. Targon ◽  
M. A. Takita ◽  
J. D. De Negri ◽  
J. Pompeu ◽  
...  
Keyword(s):  
Polymerase Chain Reaction Amplification ◽  
The Philippines ◽  
Sao Paulo ◽  
The State ◽  
Nutritional Deficiencies ◽  
São Paulo ◽  
Candidatus Liberibacter Asiaticus ◽  
First Report ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus

Huanglongbing (ex-greening) disease is one of the most serious diseases of citrus. It is caused by the phloem-limited, gram-negative bacterium “Candidatus Liberibacter spp.”. This bacterium is not well characterized mainly because it is still uncultured. There are two known strains, Asian (“Candidatus Liberibacter asiaticus”) and African (“Candidatus Liberibacter africanus”) that cause severe damage to citrus plants including twig dieback, decline, and death. Symptoms first appear as leaf mottling and chlorosis occurring in one shoot or sector of trees. Later, leaf symptoms resemble nutritional deficiencies (Zn, Ca, and N) that vary depending on the strains, with more severe symptoms caused by “Ca. L. asiaticus”. The Asian strains are transmitted by the Asian citrus psyllid (AsCP), Diaphorina citri, which is present in Brazil. The bacterium has been detected in citrus plants in many geographic locations including China, Japan, Thailand, India, the Philippines, the Arabian Peninsula, and Africa. In 2004, plants showing Huanglongbing symptoms were observed in the Araraquara County, a central region of the State of Sao Paulo, the largest citrus-producing area in Brazil. To verify the presence of “Ca. L. spp.” in these plants, leaf samples of sweet orange cvs. Hamlin and Valencia were used for DNA extraction and polymerase chain reaction amplification using the specific OI1 and Oi2c primers (1). Amplification of the 16S rDNA was positive for 2 (cvs. Hamlin and Valencia) of 10 analyzed plants. The amplified fragments were cloned and sequenced. The amplicons obtained from both plants showed the same sequence, which differed from “Ca. L. africanus”, utilized as the positive control in the amplification experiment (27 divergent bases in 1,160). The sequences were used for BLAST searches, and the results showed identities ranging from 94.71 to 100% with “Ca. L. spp.” sequences available at the National Center for Biotechnology Information database (on-line publication). The highest scores were obtained with “Ca. L. asiaticus sequences. These analyses confirmed the presence of such agent in the State of Sao Paulo. To our knowledge, this is the first report of “Ca. L. asiaticus” in Brazil as well as elsewhere in the Americas. The significance of this report relates to the potential damage that this pathogen could cause to the citrus industry in the largest citrus-producing country in the world. It remains unclear how and when the pathogen entered Brazil. Reference: (1) S. Jagoueix et al. Mol. Cell Probes 10:43, 1996.

Influence of temperature and rainfall on the population dynamics of Glycaspis brimblecombei and Psyllaephagus bliteus in Eucalyptus camaldulensis plantations

2018 ◽  
Vol 43 (1) ◽  
pp. 1 ◽  
Author(s):  
Pedro José Ferreira-Filho ◽  
Carlos Frederico Wilcken ◽  
Marcus Vinicius Masson ◽  
Wagner De Souza Tavares ◽  
Julio César Guerreiro ◽  
...  
Keyword(s):  
Population Dynamics ◽  
Eucalyptus Camaldulensis ◽  
Sao Paulo ◽  
Effect Of Temperature ◽  
Sticky Traps ◽  
Influence Of Temperature ◽  
Paulo State ◽  
Glycaspis Brimblecombei ◽  
São Paulo State ◽  
Yellow Sticky Traps

The work analyzes the effect of temperature and rainfall on the population dynamics of Glycaspis brimblecombei (Hemiptera: Aphalaridae) and its parasitoid Psyllaephagus bliteus (Hymenoptera: Encyrtidae) in a Eucalyptus camaldulensis (Myrtaceae) plantation in São Paulo State, Brazil. During the study 53 yellow sticky traps were installedand 53 branches were collected from a 19 ha plot of E. camaldulensis plantation per evaluation. A total of 42 evaluations was made from January 2006 to 2008. The number of adults of G. brimblecombei and P. bliteus caught in the yellow sticky traps as well as the immature and mummies of the parasitized psyllids on E. camaldulensis leaves varied with seasons. The population also varies according to the season, with a peak in the winter and decreases in the summer when temperature and rainfall increase. The G. bramble combei and P. bliteus populations were inversely correlated with temperature and rainfall. These results are discussed as potential limitations to the establishment of the pest and its parasitoid.

Implantação da Residência Médica e Multiprofssional em Saúde da Família em um município paulista: percepção de residentes da primeira turma (2014-2016)

2018 ◽  
Vol 19 (2) ◽  
pp. 106-114 ◽  
Author(s):  
Liliane Maria Guimarães de Pinho ◽  
Vera Lúcia Garcia ◽  
Maria Cezira Fantini Nogueira-Martins
Keyword(s):  
Family Health ◽  
Sao Paulo ◽  
São Paulo ◽  
Paulo State ◽  
São Paulo State

Implementation of a multiprofessional residency in family health in a city of São Paulo state: perception of the first residents (2014-2016)

Sign in / Sign up

Export Citation Format

Share Document