scholarly journals Effect of Chrysanthemum zawadskii Extract on Dermal Papilla Cell Proliferation and Hair Growth

2020 ◽  
Vol 32 (5) ◽  
pp. 395
Author(s):  
Youn-Duk Kim ◽  
Long-Quan Pi ◽  
Won-Soo Lee
Keyword(s):  
Cell Proliferation ◽  
Hair Growth ◽  
Dermal Papilla ◽  
Dermal Papilla Cell ◽  
Chrysanthemum Zawadskii

Transfection of rat dermal papilla cells with a gene encoding a temperature-sensitive polyomavirus large T antigen generates cell lines retaining a differentiated phenotype

1994 ◽  
Vol 107 (7) ◽  
pp. 1761-1772
Author(s):  
W. Filsell ◽  
J.C. Little ◽  
A.J. Stones ◽  
S.P. Granger ◽  
S.A. Bayley
Keyword(s):  
Cell Lines ◽  
Hair Growth ◽  
Dermal Papilla ◽  
Growth Cycle ◽  
T Antigen ◽  
Temperature Sensitive ◽  
Early Passage ◽  
Gene Encoding ◽  
Dermal Papilla Cells ◽  
Dermal Papilla Cell

The dermal papilla is a discrete group of cells at the base of the hair follicle and is implicated in controlling the hair growth cycle. Early passage dermal papilla cells can induce hair growth in vivo, but, upon further culturing, this property is lost. In order to study the events occurring in hair induction, a representative dermal papilla cell line was required. We have transfected passage 1 rat vibrissa dermal papilla cells with a polyomavirus large T gene encoding a temperature-sensitive T antigen, and generated permanent cell lines in which the immortalizing function can be switched off by temperature shift. The cells established without crisis, resembled cells in the starting population, and retained the aggregative properties of early passage dermal papilla cells. Growth studies were performed on the immortalized cell lines, which showed that transferring the cells to the restrictive temperature for the large T gene product resulted in cell senescence or quiescence, and changes in morphology. Implantation of cell pellets into the ears of immunologically compatible rats showed that the immortal cells retained hair-inductive ability. Cytokines are believed to have an important role in the control of hair growth. The pattern of cytokine gene expression in the immortal cell lines was compared with early passage dermal papilla cells and a non-hair-inducing dermal papilla cell line, using reverse transcriptase-polymerase chain reaction. Epidermal growth factor, tumour necrosis factor, and interleukin-1a were detected in the immortalized and non-hair-inducing dermal papilla cell lines, but were absent in passage 2 dermal papilla cells. All other cytokines examined were detected in all the cell types under study. These results demonstrate that the polyomavirus large Ttsa-immortalized dermal papilla cell lines are very similar to passage 2 dermal papilla cells and thus provide a good model for hair growth studies. Cytokine expression profiles indicate that the expression of several cytokines may be implicated in hair induction. Further studies are under way to investigate the relationship between cytokine expression and the hair growth cycle.

Triterpenes in the Ethanol Extract of Poria cocos Induce Dermal Papilla Cell Proliferation

2019 ◽  
Vol 16 (1) ◽  
pp. 1-9
Author(s):  
Chun-Chieh Wang ◽  
Lu-Kai Wang ◽  
Mao-Liang Chen ◽  
Chan-Yen Kuo ◽  
Fu-Ming Tsai ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Ethanol Extract ◽  
Dermal Papilla ◽  
Poria Cocos ◽  
Dermal Papilla Cell

Combination of herbal extracts and platelet-rich plasma induced dermal papilla cell proliferation: involvement of ERK and Akt pathways

2013 ◽  
Vol 12 (2) ◽  
pp. 116-122 ◽  
Author(s):  
Hosein Rastegar ◽  
Hamidreza Ahmadi Ashtiani ◽  
Mahmoud Aghaei ◽  
Amirohushang Ehsani ◽  
Behrooz Barikbin
Keyword(s):  
Cell Proliferation ◽  
Platelet Rich Plasma ◽  
Dermal Papilla ◽  
Herbal Extracts ◽  
Dermal Papilla Cell

scholarly journals miR-140-5p in Small Extracellular Vesicles From Human Papilla Cells Stimulates Hair Growth by Promoting Proliferation of Outer Root Sheath and Hair Matrix Cells

2020 ◽  
Vol 8 ◽  
Author(s):  
Yuxin Chen ◽  
Junfei Huang ◽  
Zhen Liu ◽  
Ruosi Chen ◽  
Danlan Fu ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Extracellular Vesicles ◽  
Hair Growth ◽  
Dermal Papilla ◽  
Luciferase Reporter ◽  
Outer Root Sheath ◽  
Dermal Papilla Cells ◽  
Root Sheath ◽  
Hair Matrix ◽  
Low Passage

The application of dermal papilla cells to hair follicle (HF) regeneration has attracted a great deal of attention. However, cultured dermal papilla cells (DPCs) tend to lose their capacity to induce hair growth during passage, restricting their usefulness. Accumulating evidence indicates that DPCs regulate HF growth mainly through their unique paracrine properties, raising the possibility of therapies based on extracellular vesicles (EVs). In this study, we explored the effects of EVs from high- and low-passage human scalp follicle dermal papilla cells (DP-EVs) on activation of hair growth, and investigated the underlying mechanism. DP-EVs were isolated by ultracentrifugation and cultured with human scalp follicles, hair matrix cells (MxCs), and outer root sheath cells (ORSCs), and we found low-passage DP-EVs accelerated HF elongation and cell proliferation activation. High-throughput miRNA sequencing and bioinformatics analysis identified 100 miRNAs that were differentially expressed between low- (P3) and high- (P8) passage DP-EVs. GO and KEGG pathway analysis of 1803 overlapping target genes revealed significant enrichment in the BMP/TGF-β signaling pathways. BMP2 was identified as a hub of the overlapping genes. miR-140-5p, which was highly enriched in low-passage DP-EVs, was identified as a potential regulator of BMP2. Direct repression of BMP2 by miR-140-5p was confirmed by dual-luciferase reporter assay. Moreover, overexpression and inhibition of miR-140-5p in DP-EVs suppressed and increased expression of BMP signaling components, respectively, indicating that this miRNA plays a critical role in hair growth and cell proliferation. DP-EVs transport miR-140-5p from DPCs to epithelial cells, where it downregulates BMP2. Therefore, DPC-derived vesicular miR-140-5p represents a therapeutic target for alopecia.

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