scholarly journals Various Wavelengths of Light-Emitting Diode Light Regulate the Proliferation of Human Dermal Papilla Cells and Hair Follicles via Wnt/β-Catenin and the Extracellular Signal-Regulated Kinase Pathways

2017 ◽  
Vol 29 (6) ◽  
pp. 747 ◽  
Author(s):  
Hong Jin Joo ◽  
Kwan Ho Jeong ◽  
Jung Eun Kim ◽  
Hoon Kang
Keyword(s):  
Light Emitting Diode ◽  
Dermal Papilla ◽  
Hair Follicles ◽  
Dermal Papilla Cells ◽  
Light Emitting ◽  
Extracellular Signal Regulated Kinase ◽  
Extracellular Signal

scholarly journals Extracellular Signal-Regulated Kinase Mediates Ebastine-Induced Human Follicle Dermal Papilla Cell Proliferation

2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
Fu-Ming Tsai ◽  
Chao-Hsu Li ◽  
Lu-Kai Wang ◽  
Mao-Liang Chen ◽  
Ming-Cheng Lee ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Allergic Inflammation ◽  
Dermal Papilla ◽  
Dermal Papilla Cells ◽  
Expression Levels ◽  
Extracellular Signal Regulated Kinase ◽  
Hair Regrowth ◽  
Extracellular Signal ◽  
Elevated Expression ◽  
H1 Receptor Antagonist

Ebastine is a second-generation histamine H1 receptor antagonist that is used to attenuate allergic inflammation. Ebastine has also shown to affect hair loss; however, the immunoregulatory effect of ebastine cannot completely exclude the possibility of spontaneous hair regrowth in ebastine-treated mice. In this study, we examined the effects of ebastine on the growth of human follicle dermal papilla cells (HFDPC) using a WST-1 cell proliferation assay and a bromodeoxyuridine incorporation assay. Ebastine was shown to significantly increase the proliferation of HFDPC. The expression levels of cell-cycle regulatory proteins and an antiapoptotic protein were increased in ebastine-treated HFDPC. Furthermore, elevated expression levels of phospho-AKT and phospho-p44/42 extracellular signal-regulated kinase (ERK) were observed in ebastine-treated HFDPC. Ebastine-mediated HFDPC growth was completely reversed by blocking ERK kinase. The results from our present study suggest that the regulation of HFDPC proliferation by ebastine might be directly involved in hair regrowth through the ERK signaling pathway.

Activation of the extracellular signal-regulated kinase signal pathway by light emitting diode irradiation

2010 ◽  
Vol 25 (4) ◽  
pp. 531-537 ◽  
Author(s):  
Nobuhiko Komine ◽  
Kazuo Ikeda ◽  
Kaoru Tada ◽  
Noriyuki Hashimoto ◽  
Naotoshi Sugimoto ◽  
...  
Keyword(s):  
Light Emitting Diode ◽  
Signal Pathway ◽  
Light Emitting ◽  
Extracellular Signal Regulated Kinase ◽  
Extracellular Signal

Hormones and Hair Growth: Variations in Androgen Receptor Content of Dermal papilla Cells Cultured from Human and Red Deer (Cervus Elaphus) Hair Follicles.

1993 ◽  
Vol 101 (s1) ◽  
pp. 114S-120S ◽  
Author(s):  
Valerie Anne Randall ◽  
Margaret Julie Thornton ◽  
Andrew Guy Messenger ◽  
Nigel Andrew Hibberts ◽  
Andrew Stewart Irving Loudon ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Cervus Elaphus ◽  
Hair Growth ◽  
Red Deer ◽  
Dermal Papilla ◽  
Hair Follicles ◽  
Dermal Papilla Cells ◽  
Cells Cultured

Smooth muscle alpha-actin is a marker for hair follicle dermis in vivo and in vitro

1991 ◽  
Vol 99 (3) ◽  
pp. 627-636 ◽  
Author(s):  
C.A. Jahoda ◽  
A.J. Reynolds ◽  
C. Chaponnier ◽  
J.C. Forester ◽  
G. Gabbiani
Keyword(s):  
Smooth Muscle ◽  
Hair Follicle ◽  
Dermal Papilla ◽  
Hair Follicles ◽  
Dermal Papilla Cells ◽  
Smooth Muscle Alpha Actin ◽  
Actin Expression ◽  
Alpha Actin ◽  
Sheath Cells

We have examined the expression of smooth muscle alpha-actin in hair follicles in situ, and in hair follicle dermal cells in culture by means of immunohistochemistry. Smooth muscle alpha-actin was present in the dermal sheath component of rat vibrissa, rat pelage and human follicles. Dermal papilla cells within all types of follicles did not express the antigen. However, in culture a large percentage of both hair dermal papilla and dermal sheath cells were stained by this antibody. The same cells were negative when tested with an antibody to desmin. Overall, explant-derived skin fibroblasts had relatively low numbers of positively marked cells, but those from skin regions of high hair-follicle density displayed more smooth muscle alpha-actin expression than fibroblasts from areas with fewer follicles. 2-D SDS-PAGE confirmed that, unlike fibroblasts, cultured papilla cells contained significant quantities of the alpha-actin isoform. The rapid switching on of smooth muscle alpha-actin expression by dermal papilla cells in early culture, contrasts with the behaviour of smooth muscle cells in vitro, and has implications for control of expression of the antigen in normal adult systems. The very high percentage of positively marked cultured papilla and sheath cells also provides a novel marker of cells from follicle dermis, and reinforces the idea that they represent a specialized cell population, contributing to the heterogeneity of fibroblast cell types in the skin dermis, and possibly acting as a source of myofibroblasts during wound healing.

scholarly journals Inhibition of Rab27a and Rab27b Has Opposite Effects on the Regulation of Hair Cycle and Hair Growth

2020 ◽  
Vol 21 (16) ◽  
pp. 5672
Author(s):  
Kyung-Eun Ku ◽  
Nahyun Choi ◽  
Jong-Hyuk Sung
Keyword(s):  
Hair Growth ◽  
Expression Patterns ◽  
Dermal Papilla ◽  
Hair Follicles ◽  
Hair Cycle ◽  
Outer Root Sheath ◽  
Dermal Papilla Cells ◽  
Root Sheath ◽  
Culture Models

Rab27a/b are known to play an important role in the transport of melanosomes, with their knockout causing silvery gray hair. However, the relationship between Rab27a/b and hair growth is not well known. To evaluate the role of Rab27a/b in hair cycle, we investigated the expression of Rab27a/b during hair cycling and human outer root sheath (hORS) cells. The expression of Rab27a in ORS cells was mainly detected at the anagen, whereas expression of Rab27b in ORS, and epidermal cells was strongly expressed at the telogen. Additionally, Rab27a/b were expressed in the Golgi of hORS cells. To evaluate the role of Rab27a/b in hair growth, telogen-to-anagen transition animal and vibrissae hair follicles (HFs) organ culture models were assayed using Rab27a/b siRNAs. The knockdown of Rab27a or Rab27b suppressed or promoted hair growth, respectively. These results were also confirmed in human dermal papilla cells (hDPCs) and hORS cells, showing the opposite mitogenic effects. Moreover, Rab27b knockdown increased the expression levels of various growth factors in the hDPCs and hORS cells. Overall, the opposite temporal expression patterns during hair cycling and roles for hair growth of Rab27a/b suggested that Rab27a/b might regulate the hair cycle. Therefore, our study may provide a novel solution for the development of hair loss treatment by regulating Rab27a/b levels.

scholarly journals The local hypothalamic–pituitary–adrenal axis in cultured human dermal papilla cells

2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Eun Young Lee ◽  
You Jin Nam ◽  
Sangjin Kang ◽  
Eun Ju Choi ◽  
Inbo Han ◽  
...  
Keyword(s):  
Hpa Axis ◽  
Hair Loss ◽  
Human Hair ◽  
Stress Hormones ◽  
Dermal Papilla ◽  
Hair Shaft ◽  
Hair Follicles ◽  
Follicle Cells ◽  
Dermal Papilla Cells ◽  
Underlying Mechanisms

Abstract Background Stress is an important cause of skin disease, including hair loss. The hormonal response to stress is due to the HPA axis, which comprises hormones such as corticotropin releasing factor (CRF), adrenocorticotropic hormone (ACTH), and cortisol. Many reports have shown that CRF, a crucial stress hormone, inhibits hair growth and induces hair loss. However, the underlying mechanisms are still unclear. The aim of this study was to examine the effect of CRF on human dermal papilla cells (DPCs) as well as hair follicles and to investigate whether the HPA axis was established in cultured human DPCs. Results CRF inhibited hair shaft elongation and induced early catagen transition in human hair follicles. Hair follicle cells, both human DPCs and human ORSCs, expressed CRF and its receptors and responded to CRF. CRF inhibited the proliferation of human DPCs through cell cycle arrest at G2/M phase and induced the accumulation of reactive oxygen species (ROS). Anagen-related cytokine levels were downregulated in CRF-treated human DPCs. Interestingly, increases in proopiomelanocortin (POMC), ACTH, and cortisol were induced by CRF in human DPCs, and antagonists for the CRF receptor blocked the effects of this hormone. Conclusion The results of this study showed that stress can cause hair loss by acting through stress hormones. Additionally, these results suggested that a fully functional HPA axis exists in human DPCs and that CRF directly affects human DPCs as well as human hair follicles under stress conditions.

Metabolism of Testosterone by Cultured Dermal Papilla Cells from Human Beard, Pubic, and Scalp Hair Follicles

2006 ◽  
Vol 642 (1) ◽  
pp. 452-453 ◽  
Author(s):  
M. J. THORNTON ◽  
K. HAMADA ◽  
I. LAING ◽  
A. G. MESSENGER ◽  
V. A. RANDALL
Keyword(s):  
Scalp Hair ◽  
Dermal Papilla ◽  
Hair Follicles ◽  
Dermal Papilla Cells

Androgen Receptors in Dermal Papilla Cells of Scalp Hair Follicles in Male Pattern Baldness

2006 ◽  
Vol 642 (1) ◽  
pp. 448-451 ◽  
Author(s):  
M. B. HODGINS ◽  
R. CHOUDHRY ◽  
G. PARKER ◽  
R. F. OLIVER ◽  
C. A. B. JAHODA ◽  
...  
Keyword(s):  
Androgen Receptors ◽  
Scalp Hair ◽  
Dermal Papilla ◽  
Hair Follicles ◽  
Dermal Papilla Cells ◽  
Male Pattern Baldness ◽  
Male Pattern

Effect of Androgens on the Growth of Cultured Human Dermal Papilla Cells Derived from Beard and Scalp Hair Follicles

1991 ◽  
Vol 97 (2) ◽  
pp. 345-348 ◽  
Author(s):  
M Julie Thornton ◽  
Andrew G Messenger ◽  
Katherine Elliott ◽  
Valerie A Randall
Keyword(s):  
Scalp Hair ◽  
Dermal Papilla ◽  
Hair Follicles ◽  
Dermal Papilla Cells
Sign in / Sign up

Export Citation Format

Share Document