scholarly journals Intense Pulsed Light Increases Hyaluronan and CD44 in Epidermal Keratinocytes and Improves Age-Related Epidermal Structure Defects in Mice

2017 ◽  
Vol 29 (3) ◽  
pp. 377
Author(s):  
Sang Eun Lee ◽  
Hye Rang On ◽  
Jung Min Lim ◽  
Sang Jin Kang ◽  
Ji-Ye Song ◽  
...  
Keyword(s):  
Intense Pulsed Light ◽  
Epidermal Keratinocytes ◽  
Pulsed Light ◽  
Epidermal Structure ◽  
Structure Defects ◽  
Age Related

scholarly journals Intense Pulsed Light Attenuates UV-Induced Hyperimmune Response and Pigmentation in Human Skin Cells

2021 ◽  
Vol 22 (6) ◽  
pp. 3173
Author(s):  
Juewon Kim ◽  
Jeongin Lee ◽  
Hyunjung Choi
Keyword(s):  
Oxidative Stress ◽  
Enzyme Activity ◽  
Combined Treatment ◽  
Antioxidative Enzyme ◽  
Intense Pulsed Light ◽  
Epidermal Keratinocytes ◽  
Pulsed Light ◽  
Uvb Irradiation ◽  
Skin Cells ◽  
Antioxidative Enzyme Activity

The skin of an organism is affected by various environmental factors and fights against aging stress via mechanical and biochemical responses. Photoaging induced by ultraviolet B (UVB) irradiation is common and is the most vital factor in the senescence phenotype of skin, and so, suppression of UVB stress-induced damage is critical. To lessen the UVB-induced hyperimmune response and hyperpigmentation, we investigated the ameliorative effects of intense pulsed light (IPL) treatment on the photoaged phenotype of skin cells. Normal human epidermal keratinocytes and human epidermal melanocytes were exposed to 20 mJ/cm2 of UVB. After UVB irradiation, the cells were treated with green (525–530 nm) and yellow (585–592 nm) IPL at various time points prior to the harvest step. Subsequently, various signs of excessive immune response, including expression of proinflammatory and melanogenic genes and proteins, cellular oxidative stress level, and antioxidative enzyme activity, were examined. We found that IPL treatment reduced excessive cutaneous immune reactions by suppressing UVB-induced proinflammatory cytokine expression. IPL treatment prevented hyperpigmentation, and combined treatment with green and yellow IPL synergistically attenuated both processes. IPL treatment may exert protective effects against UVB injury in skin cells by attenuating inflammatory cytokine and melanogenic gene overexpression, possibly by reducing intracellular oxidative stress. IPL treatment also preserves antioxidative enzyme activity under UVB irradiation. This study suggests that IPL treatment is a useful strategy against photoaging, and provides evidence supporting clinical approaches with non-invasive light therapy.

scholarly journals Decontamination of Powdery Foods Using an Intense Pulsed Light (IPL) Device for Practical Application

2021 ◽  
Vol 11 (4) ◽  
pp. 1518
Author(s):  
Hee-Jeong Hwang ◽  
So-Yoon Yee ◽  
Myong-Soo Chung
Keyword(s):  
Total Energy ◽  
Water Activity ◽  
Treatment Time ◽  
Sesame Seed ◽  
Pilot Scale ◽  
Black Pepper ◽  
Intense Pulsed Light ◽  
Pulsed Light ◽  
Energy Fluence ◽  
Color Changes

Controlling microbial problems when processing seeds and powdered foods is difficult due to their low water activity, irregular surfaces, and opaqueness. Moreover, existing thermal processing can readily cause various undesirable changes in sensory properties. Intense pulsed light (IPL) can be effective in nonthermal processing, and so two xenon lamps were attached to the sides of a self-designed cyclone type of pilot-scale IPL device. Each lamp was connected to its own power supply, and the following treatment conditions were applied to four sample types: lamp DC voltage of 1800–4200 V, pulse width of 0.5–1.0 ms, frequency of 2 Hz, and treatment time of 1–5 min. This device achieved reductions of 0.45, 0.66, and 0.88 log CFU/mL for ground black pepper, red pepper, and embryo buds of rice, respectively, under a total energy fluence of 12.31 J/cm2. Meanwhile, >3-log reductions were achieved for sesame seed samples under a total energy fluence of 11.26 J/cm2. In addition, analyses of color changes, water activity, and moisture content revealed no significant differences between the control and IPL-treated samples. These findings indicate that IPL treatment may be considered a feasible sterilization method for seeds and powdered foods.

scholarly journals EGFR inhibitors switch keratinocytes from a proliferative to a differentiative phenotype affecting epidermal development and barrier function

BMC Cancer ◽  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Nicolas Joly-Tonetti ◽  
Thomas Ondet ◽  
Mario Monshouwer ◽  
Georgios N. Stamatas
Keyword(s):  
Growth Factor ◽  
Barrier Function ◽  
Kinase Inhibitors ◽  
Treatment Protocol ◽  
Growth Factor Receptor ◽  
Skin Barrier ◽  
Keratinocyte Differentiation ◽  
Skin Barrier Function ◽  
Epidermal Keratinocytes ◽  
Epidermal Structure

Abstract Background Cutaneous adverse drug reactions (CADR) associated with oncology therapy involve 45–100% of patients receiving kinase inhibitors. Such adverse reactions may include skin inflammation, infection, pruritus and dryness, symptoms that can significantly affect the patient’s quality of life. To prevent severe skin damages dose adjustment or drug discontinuation is often required, interfering with the prescribed oncology treatment protocol. This is particularly the case of Epidermal Growth Factor Receptor inhibitors (EGFRi) targeting carcinomas. Since the EGFR pathway is pivotal for epidermal keratinocytes, it is reasonable to hypothesize that EGFRi also affect these cells and therefore interfere with the epidermal structure formation and skin barrier function. Methods To test this hypothesis, the effects of EGFRi and Vascular Endothelial Growth Factor Receptor inhibitors (VEGFRi) at therapeutically relevant concentrations (3, 10, 30, 100 nM) were assessed on proliferation and differentiation markers of human keratinocytes in a novel 3D micro-epidermis tissue culture model. Results EGFRi directly affect basal keratinocyte growth, leading to tissue size reduction and switching keratinocytes from a proliferative to a differentiative phenotype, as evidenced by decreased Ki67 staining and increased filaggrin, desmoglein-1 and involucrin expression compared to control. These effects lead to skin barrier impairment, which can be observed in a reconstructed human epidermis model showing a decrease in trans-epidermal water loss rates. On the other hand, pan-kinase inhibitors mainly targeting VEGFR barely affect keratinocyte differentiation and rather promote a proliferative phenotype. Conclusions This study contributes to the mechanistic understanding of the clinically observed CADR during therapy with EGFRi. These in vitro results suggest a specific mode of action of EGFRi by directly affecting keratinocyte growth and barrier function.

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