scholarly journals Interplay between Brassinosteroid and ABA signaling during early seedling development

2017 ◽  
Vol 44 (3) ◽  
pp. 264-270 ◽  
Author(s):  
Hyemin Kim ◽  
Jeongeui Hong ◽  
Yong-Gu Cho ◽  
Kwon Kyoo Kang ◽  
Hojin Ryu
Keyword(s):  
Seedling Development ◽  
Aba Signaling ◽  
Early Seedling ◽  
Early Seedling Development

scholarly journals O-fucosylation of CPN20 by SPINDLY Derepresses Abscisic Acid Signaling During Seed Germination and Seedling Development

2021 ◽  
Vol 12 ◽  
Author(s):  
Lin Liang ◽  
Qi Wang ◽  
Zihao Song ◽  
Yaxin Wu ◽  
Qing Liang ◽  
...  
Keyword(s):  
Seed Germination ◽  
Mammalian Cells ◽  
Electron Transfer Dissociation ◽  
Seedling Development ◽  
Aba Signaling ◽  
Early Seedling ◽  
Aba Insensitive ◽  
Early Seedling Development

SPINDLY is involved in some aspects of plant development. However, the nature of this protein as an O-fucosyltransferase was recently discovered. In this study, we show that SPINDLY (SPY) interacts with CPN20 in yeast two-hybrid and split-luc assays, and the interaction is promoted by ABA. CPN20 is a chloroplast-localized co-chaperonin that negatively regulates ABAR-mediated ABA signaling. By using Electron Transfer Dissociation-MS/MS analysis, two O-fucosylation sites, e.g., 116th and 119th threonines, were detected in ectopically expressed CPN20 in mammalian cells and in Arabidopsis. The O-fucosylation at both threonine residues was confirmed by in vitro peptide O-fucosylation assay. We further show that CPN20 accumulates in the chloroplast of spy mutants, suggesting that SPY negatively regulates CPN20 localization in the chloroplast. In vivo protein degradation assay along with CPN20 localization behavior suggest that import of CPN20 into the chloroplast is negatively regulated by SPY. Genetic analysis shows that ABA insensitive phenotypes of spy-3 in terms of seed germination and early seedling development are partially suppressed by the cpn20 mutation, suggesting that CPN20 acts downstream of SPY in this ABA signaling pathway and that there may exist other pathways in parallel with CPN20. Collectively, the above data support the notion that the O-fucosylation of CPN20 by SPY fine-tunes ABA signaling in Arabidopsis.

scholarly journals Zinc Seed Priming Improves Spinach Germination at Low Temperature

Agriculture ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 271
Author(s):  
Muhammad Imran ◽  
Asim Mahmood ◽  
Günter Neumann ◽  
Birte Boelt
Keyword(s):  
Low Temperature ◽  
Temperature Stress ◽  
Multispectral Imaging ◽  
Germination Rate ◽  
Cost Effective ◽  
Seed Priming ◽  
Seedling Development ◽  
Low Temperature Stress ◽  
Early Seedling ◽  
Early Seedling Development

Low temperature during germination hinders germination speed and early seedling development. Zn seed priming is a useful and cost-effective tool to improve germination rate and resistance to low temperature stress during germination and early seedling development. Spinach was tested to improve germination and seedling development with Zn seed priming under low temperature stress conditions. Zn priming increased seed Zn concentration up to 48 times. The multispectral imaging technique with VideometerLab was used as a non-destructive method to differentiate unprimed, water- and Zn-primed spinach seeds successfully. Localization of Zn in the seeds was studied using the 1,5-diphenyl thiocarbazone (DTZ) dying technique. Active translocation of primed Zn in the roots of young seedlings was detected with laser confocal microscopy. Zn priming of spinach seeds at 6 mM Zn showed a significant increase in germination rate and total germination under low temperature at 8 °C.

scholarly journals Germination and Early Seedling Development in Quercus ilex Recalcitrant and Non-dormant Seeds: Targeted Transcriptional, Hormonal, and Sugar Analysis

2018 ◽  
Vol 9 ◽  
Author(s):  
M. Cristina Romero-Rodríguez ◽  
Antonio Archidona-Yuste ◽  
Nieves Abril ◽  
Antonio M. Gil-Serrano ◽  
Mónica Meijón ◽  
...  
Keyword(s):  
Quercus Ilex ◽  
Seedling Development ◽  
Sugar Analysis ◽  
Early Seedling ◽  
Early Seedling Development

scholarly journals Components of the Arabidopsis mRNA Decapping Complex Are Required for Early Seedling Development

2007 ◽  
Vol 19 (5) ◽  
pp. 1549-1564 ◽  
Author(s):  
David C. Goeres ◽  
Jaimie M. Van Norman ◽  
Weiping Zhang ◽  
Nellie A. Fauver ◽  
Mary Lou Spencer ◽  
...  
Keyword(s):  
Seedling Development ◽  
Mrna Decapping ◽  
Early Seedling ◽  
Early Seedling Development
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