Regenerating of consuming ion-exchange resin by graft polymerization for steam boiler wastewater treatment

2021 ◽  
Vol 222 ◽  
pp. 127-136
Author(s):  
Hadi S. Al-Lami ◽  
Mahmood A. Al-Marzok
Keyword(s):  
Wastewater Treatment ◽  
Ion Exchange ◽  
Graft Polymerization ◽  
Exchange Resin ◽  
Ion Exchange Resin ◽  
Steam Boiler

Removal of Fluorine and Boron From Groundwater Using Radiation-Induced Graft Polymerization Adsorbent at Mizunami Underground Research Laboratory

2010 ◽  
Author(s):  
Yosuke Iyatomi ◽  
Hiroyuki Hoshina ◽  
Noriaki Seko ◽  
Noboru Kasai ◽  
Yuji Ueki ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Research Laboratory ◽  
Graft Polymerization ◽  
Exchange Resin ◽  
Ion Exchange Resin ◽  
Boron Removal ◽  
Radiation Induced ◽  
Flow Through ◽  
Underground Research Laboratory ◽  
Better Than

High fluorine and boron contents in groundwater are commonly reduced using coagulation and ion-exchange treatments. As an alternative, we tested the efficiency of fluorine and boron removal from groundwater using radiation-induced graft polymerization adsorbent. The durability of the adsorbent was also determined by varying groundwater flow-through rates and repetitive use of the adsorbent. The results indicated that it was possible for the adsorbent to remove more than 95% of boron and fluorine from the groundwater, and that the performance of the adsorbent for boron removal was better than commonly used ion-exchange resin. The adsorbent used several times was able to remove boron, indicating that the adsorbent can be used for efficient boron removal.

Preparation of ion exchange resin using soluble starch and acrylamide by graft polymerization and hydrolysis

2018 ◽  
Vol 26 (4) ◽  
pp. 3803-3813 ◽  
Author(s):  
Ming Zhang ◽  
Guihong Lan ◽  
Haiyan Qiu ◽  
Tailiang Zhang ◽  
Wenjing Li ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Graft Polymerization ◽  
Exchange Resin ◽  
Ion Exchange Resin ◽  
Soluble Starch

OPTIMIZATION OF DNA EXTRACTION METHODS FROM BIOLOGICAL MATERIALS OF HONEY BEES IN A DIFFERENT METAMOTPHOSIS PHASE

2016 ◽  
Vol 52 ◽  
pp. 171-176
Author(s):  
M. Palkina ◽  
O. Metlitska
Keyword(s):  
Ion Exchange ◽  
Dna Extraction ◽  
Honey Bees ◽  
Biological Material ◽  
Exchange Resin ◽  
Ion Exchange Resin ◽  
Extraction Methods ◽  
Chelex 100 ◽  
Drone Brood ◽  
Dna Extraction Methods

The aim of the research – adaptation, optimization and using of existing DNA extraction methods from bees’ biological material with the reagent «Chelex-100" under complex economic conditions of native laboratories, which will optimize labour costs and improve the economic performance of DNA extraction protocol. Materials and methods. In order to conduct the research the samples of honey bees’ biological material: queen pupae exuviae, larvae of drone brood, some adult bees’ bodies (head and thorax) were selected. Bowl and drone brood were obtained from the experimental bee hives of Institute of Apiculture nd. a. P. I. Prokopovich of NAAS. DNA extraction from biosamples of Apis mellifera ssp. was carried out using «Chelex-100®» ion exchange resin in different concentrations and combinations. Before setting tests for determination of quantitative and quality indexes, dilution of DNA samples of the probed object was conducted in ratio 1:40. The degree of contamination with protein and polysaccharide fractions (OD 260/230), quantitative content of DNA (OD 260/280) in the extracted tests were conducted using spectrophotometer of «Biospec – nano» at the terms of sample volume in 2 µl and length of optical way in 0,7 mm [7]. Verification of DNA samples from biological material of bees, isolated by «Chelex-100®», was conducted after cold keeping during 24 hours at 20°C using PСR with primaries to the fragment of gene of quantitative trait locus (QTL) Sting-2 of next structure [8]:  3' – CTC GAC GAG ACG ACC AAC TTG – 5’; 3' – AAC CAG AGT ATC GCG AGT GTT AC – 5’ Program of amplification: 94 °C – 5 minutes – 1 cycle; 94 °C – 1 minute, 57°C – 1 minute, 72 °C – 2 minutes – 30 cycles; elongation after 72°C during 2 minutes – 1 cycle. The division of obtained amplicons was conducted by gel electrophoresis at a low current – 7 µÀ, in 1,5 % agarose gel (Sigma ®) in TAE buffer [7]. The results. At the time of optimization of DNA isolation methods, according to existing methods of foreign experts, it was found optimal volume of ion exchange resin solution was in the proposed concentration: instead of 60 µl of solution used 120 µl of «Chelex-100®», time of incubation was also amended from 30 minutes to 180 minutes [9]. The use of the author's combination of method «Chelex-100®» with lysis enzymes, proteinase K and detergents (1M dithiothreitol), as time of incubation was also amended, which was reduced to 180 minutes instead of the proposed 12 hours [10]. Changes in quality characteristics of obtained DNA in samples after reduction in incubation time were not found. Conclusions. The most economical method of DNA isolation from bees’ biological material is 20% solution of «Chelex-100» ion exchange resin with the duration of the incubation period of 180 minutes. It should also be noted that the best results can be obtained from exuviae, selected immediately after the queen’s exit from bowl, that reduces the likelihood of DNA molecules destruction under the influence of nucleases activation, but not later than 12 hours from release using the technology of isolated obtain of queens.

Chromatography of chlorinated biphenyls on an ion-exchange resin

1977 ◽  
Vol 49 (6) ◽  
pp. 764-766 ◽  
Author(s):  
Toshihiko. Hanai ◽  
Harold F. Walton
Keyword(s):  
Ion Exchange ◽  
Exchange Resin ◽  
Ion Exchange Resin
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