Effect of adding exogenous nutrients on refractory wastewater treatment and bacterial community in activated sludge

2018 ◽  
Vol 115 ◽  
pp. 88-96
Author(s):  
Zhaoxin Zhang ◽  
Jiake Li ◽  
Liming Song ◽  
Hongrui Ma
Keyword(s):  
Wastewater Treatment ◽  
Bacterial Community ◽  
Activated Sludge ◽  
Exogenous Nutrients

scholarly journals The capacity of wastewater treatment plants drives bacterial community structure and its assembly

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Young Kyung Kim ◽  
Keunje Yoo ◽  
Min Sung Kim ◽  
Il Han ◽  
Minjoo Lee ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Community Structure ◽  
Bacterial Community ◽  
Activated Sludge ◽  
Community Assembly ◽  
Bacterial Communities ◽  
Wastewater Treatment Plants ◽  
High Capacity ◽  
Operating Conditions ◽  
Rrna Gene

Abstract Bacterial communities in wastewater treatment plants (WWTPs) affect plant functionality through their role in the removal of pollutants from wastewater. Bacterial communities vary extensively based on plant operating conditions and influent characteristics. The capacity of WWTPs can also affect the bacterial community via variations in the organic or nutrient composition of the influent. Despite the importance considering capacity, the characteristics that control bacterial community assembly are largely unknown. In this study, we discovered that bacterial communities in WWTPs in Korea and Vietnam, which differ remarkably in capacity, exhibit unique structures and interactions that are governed mainly by the capacity of WWTPs. Bacterial communities were analysed using 16S rRNA gene sequencing and exhibited clear differences between the two regions, with these differences being most pronounced in activated sludge. We found that capacity contributed the most to bacterial interactions and community structure, whereas other factors had less impact. Co-occurrence network analysis showed that microorganisms from high-capacity WWTPs are more interrelated than those from low-capacity WWTPs, which corresponds to the tighter clustering of bacterial communities in Korea. These results will contribute to the understanding of bacterial community assembly in activated sludge processing.

scholarly journals Mobilizable IncQ-Related Plasmid Carrying a New Quinolone Resistance Gene, qnrS2, Isolated from the Bacterial Community of a Wastewater Treatment Plant

2006 ◽  
Vol 50 (9) ◽  
pp. 3075-3080 ◽  
Author(s):  
Gabriele Bönemann ◽  
Michael Stiens ◽  
Alfred Pühler ◽  
Andreas Schlüter
Keyword(s):  
Wastewater Treatment ◽  
Bacterial Community ◽  
Activated Sludge ◽  
Resistance Gene ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
The United States ◽  
Replication Initiation ◽  
Quinolone Resistance ◽  
Selection Strategy

ABSTRACT Plasmid-encoded quinolone resistance was previously reported for different bacteria isolated from patients not only in the United States and Asia but also in Europe. Here we describe the isolation, by applying a new selection strategy, of the quinolone resistance plasmid pGNB2 from an activated sludge bacterial community of a wastewater treatment plant in Germany. The hypersensitive Escherichia coli strain KAM3 carrying a mutation in the multidrug efflux system genes acrAB was transformed with total plasmid DNA preparations isolated from activated sludge bacteria and subsequently selected on medium containing the fluoroquinolone norfloxacin. This approach resulted in the isolation of plasmid pGNB2 conferring decreased susceptibility to nalidixic acid and to different fluoroquinolones. Analysis of the pGNB2 nucleotide sequence revealed that it is 8,469 bp in size and has a G+C content of 58.2%. The plasmid backbone is composed of a replication initiation module (repA-repC) belonging to the IncQ-family and a two-component mobilization module that confers horizontal mobility to the plasmid. In addition, plasmid pGNB2 carries an accessory module consisting of a transposon Tn1721 remnant and the quinolone resistance gene, qnrS2, that is 92% identical to the qnrS gene located on plasmid pAH0376 from Shigella flexneri 2b. QnrS2 belongs to the pentapeptide repeat protein family and is predicted to protect DNA-gyrase activity against quinolones. This is not only the first report on a completely sequenced plasmid mediating quinolone resistance isolated from an environmental sample but also on the first qnrS-like gene detected in Europe.

scholarly journals Impact of Operational Parameters on Bacterial Community in a Full-Scale Municipal Wastewater Treatment Plant

2012 ◽  
Vol 61 (1) ◽  
pp. 41-49 ◽  
Author(s):  
AGNIESZKA CYDZIK-KWIATKOWSKA ◽  
MAGDALENA ZIELIŃSKA ◽  
IRENA WOJNOWSKA-BARYŁA
Keyword(s):  
Wastewater Treatment ◽  
Bacterial Community ◽  
Activated Sludge ◽  
Municipal Wastewater ◽  
Treatment Plant ◽  
Organic Load ◽  
Municipal Wastewater Treatment ◽  
Load Rate ◽  
Organic Load Rate ◽  
Ammonia Oxidising Bacteria

A bacterial community in activated sludge from a full-scale municipal wastewater treatment plant was monitored throughout the year with the use of FISH, RISA and DGGE techniques. In the investigated range of temperatures (11.9-21.6 degrees C), a rise in temperature resulted in a lower total bacteria richness, while organic load rate changes from 0.09 to 0.21 g COD x g TSS(-1) x d(-1) were positively correlated with the number of bands in RISA patterns. The most diverse pattern (29 different bands) was characteristic for the activated sludge sample collected at the end of January at wastewater temperature of 11.9 degrees C. The ammonia-oxidising bacteria community did not change during the study, and comprised of 4 different bacterial populations with one dominant species closely related to Nitrosospira sp. REGAU (GenBank accession number AY635572.1). The percentage of ammonia-oxidising bacteria in the activated sludge varied from 6.2 to 19.5% and depended on temperature (R = 0.61, p = 0:02) and organic load rate (R = -0.55, p = 0.04).

scholarly journals Analysis of Bacterial Community Structure of Activated Sludge from Wastewater Treatment Plants in Winter

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Shuang Xu ◽  
Junqin Yao ◽  
Meihaguli Ainiwaer ◽  
Ying Hong ◽  
Yanjiang Zhang
Keyword(s):  
Wastewater Treatment ◽  
Community Structure ◽  
Bacterial Community ◽  
Activated Sludge ◽  
Bacterial Community Structure ◽  
Redundancy Analysis ◽  
Wastewater Treatment Plants ◽  
Effluent Treatment ◽  
Autonomous Region ◽  
Sludge Bulking

Activated sludge bulking is easily caused in winter, resulting in adverse effects on effluent treatment and management of wastewater treatment plants. In this study, activated sludge samples were collected from different wastewater treatment plants in the northern Xinjiang Uygur Autonomous Region of China in winter. The bacterial community compositions and diversities of activated sludge were analyzed to identify the bacteria that cause bulking of activated sludge. The sequencing generated 30087–55170 effective reads representing 36 phyla, 293 families, and 579 genera in all samples. The dominant phyla present in all activated sludge were Proteobacteria (26.7–48.9%), Bacteroidetes (19.3–37.3%), Chloroflexi (2.9–17.1%), and Acidobacteria (1.5–13.8%). Fifty-five genera including unclassified_f_Comamonadaceae, norank_f_Saprospiraceae, Flavobacterium, norank_f_Hydrogenophilaceae, Dokdonella, Terrimonas, norank_f_Anaerolineaceae, Tetrasphaera, Simplicispira, norank_c_Ardenticatenia, and Nitrospira existed in all samples, accounting for 60.6–82.7% of total effective sequences in each sample. The relative abundances of Saprospiraceae, Flavobacterium, and Tetrasphaera with the respective averages of 12.0%, 8.3%, and 5.2% in bulking sludge samples were higher than those in normal samples. Filamentous Saprospiraceae, Flavobacterium, and Tetrasphaera multiplied were the main cause for the sludge bulking. Redundancy analysis (RDA) indicated that influent BOD5, DO, water temperature, and influent ammonia had a distinct effect on bacterial community structures.

DESIGN PLANNING WASTEWATER TREATMENT PLANT OF NATA DE COCO INDUSTRY WITH THE ACTIVATED SLUDGE PROCESS

2016 ◽  
Vol 9 (2) ◽  
Author(s):  
Dinda Rita K. Hartaja ◽  
Imam Setiadi
Keyword(s):  
Waste Water ◽  
Wastewater Treatment ◽  
Activated Sludge ◽  
Waste Water Treatment ◽  
Treatment Plant ◽  
Water Treatment Plant ◽  
Appropriate Technology ◽  
Waste Water Treatment Plant ◽  
Activated Sludge Process ◽  
High Level

Generally, wastewater of nata de coco industry contains suspended solids and COD were high, ranging from 90,000 mg / l. The high level of of the wastewater pollutants, resulting in nata de coco industry can not be directly disposed of its wastewater into the environment agency. Appropriate technology required in order to process the waste water so that the treated water can meet the environmental quality standards that are allowed. Designing the waste water treatment plant that is suitable and efficient for treating industrial wastewater nata de coco is the activated sludge process. Wastewater treatment using activated sludge process of conventional (standard) generally consists of initial sedimentation, aeration and final sedimentation.Keywords : Activated Sludge, Design, IPAL

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