Determination of overall microbial activity in sewage treatment plants using FDA hydrolysis assay

2017 ◽  
Vol 100 ◽  
pp. 275-280
Author(s):  
Rahul S. Sutar ◽  
Dheeraj Kumar ◽  
Ketan A. Kamble ◽  
Anurag Singh ◽  
Shyam R. Asolekar
Keyword(s):  
Microbial Activity ◽  
Sewage Treatment ◽  
Sewage Treatment Plants ◽  
Fda Hydrolysis

Screening of Human Antibiotic Substances and Determination of Weekly Mass Flows in Five Sewage Treatment Plants in Sweden

2005 ◽  
Vol 39 (10) ◽  
pp. 3421-3429 ◽  
Author(s):  
Richard H. Lindberg ◽  
Patrik Wennberg ◽  
Magnus I. Johansson ◽  
Mats Tysklind ◽  
Barbro A. V. Andersson
Keyword(s):  
Sewage Treatment ◽  
Sewage Treatment Plants ◽  
Mass Flows

Influence of microbial activity on polar xenobiotic degradation in activated sludge systems

2010 ◽  
Vol 62 (3) ◽  
pp. 701-707 ◽  
Author(s):  
M. Majewsky ◽  
T. Gallé ◽  
L. Zwank ◽  
K. Fischer
Keyword(s):  
Activated Sludge ◽  
Microbial Activity ◽  
Suspended Solids ◽  
Sewage Treatment ◽  
Total Suspended Solids ◽  
Active Biomass ◽  
Sewage Treatment Plants ◽  
Degradation Rates ◽  
Xenobiotic Degradation ◽  
Activated Sludge Systems

The influence of activated sludge quality on the co-metabolic biodegradation of three aminopolycarboxyl acids was investigated for a variety of Luxembourg sewage treatment plants. A combination of biodegradation experiments and respirometric techniques are presented as a reliable approach for the estimation of biokinetics and biological xenobiotic degradation rates that allow for identification of governing parameters such as microbial activity and active biomass. Results showed that biokinetics and degradation rates vary greatly between different plants. The fraction of active biomass on the total suspended solids ranged between 16.9 and 53.7%. Xenobiotic biodegradation rates correlated with microbial activity suggesting a relationship with WWTP performance for carbon and nutrient removal. The biokinetic information can be used to increase the prediction accuracy of xenobiotics removal by individual WWTPs.

Determination of isoflavones and coumestrol in river water and domestic wastewater sewage treatment plants

2005 ◽  
Vol 531 (2) ◽  
pp. 229-237 ◽  
Author(s):  
Alessandro Bacaloni ◽  
Chiara Cavaliere ◽  
Angelo Faberi ◽  
Patrizia Foglia ◽  
Roberto Samperi ◽  
...  
Keyword(s):  
River Water ◽  
Sewage Treatment ◽  
Domestic Wastewater ◽  
Sewage Treatment Plants

scholarly journals Enzymatic Activity as a Measure of Total Microbial Activity on Historical Stone

Heritage ◽  
2020 ◽  
Vol 3 (3) ◽  
pp. 671-681
Author(s):  
Elif Sırt Çıplak ◽  
Kiraz Göze Akoğlu
Keyword(s):  
Enzymatic Activity ◽  
Microbial Activity ◽  
Plate Count ◽  
Biological Factors ◽  
Bacterial Counts ◽  
Hydrolysis Method ◽  
Deterioration Process ◽  
Plate Count Method ◽  
Fda Hydrolysis

Stones of historical monuments exposed to the open air deteriorate over the course of time depending on physical, chemical, and biological factors acting in co-association. Among the biological factors, microorganisms play a key role in the deterioration process of stones. Detecting the level of microbial activity on stones is an essential step in diagnostic and monitoring studies of stone biodeterioration, and aids in controlling the performance of treatments applied to the stones. Therefore, this study aimed to develop a practical and rapid method for the determination of microbial activity on historical stones and use this method on the Mount Nemrut monuments (MNMs) (Adiyaman, Turkey). For that purpose, the fluorescein diacetate (FDA) hydrolysis method, frequently employed for soil environments, was adapted for the estimation and assessment of total microbial activity to understand whether microorganisms posed a potential risk for the biodeterioration of the limestones and sandstones of the MNMs. The traditional plate count method was also applied simultaneously to the same stone samples to compare and assist in the interpretation of the results of the FDA hydrolysis method, which relies on the quantitative determination of bacterial and fungal colonies in nutrient agar and malt extract agar medium, respectively. The results of the FDA hydrolysis and plate count methods showed consistency. The total microbial activity determined by the FDA hydrolysis method was low for both types of stone samples. In addition, the plate count method showed low bacterial and fungal counts on all of the samples. This revealed that microbial activity did not play an important role in the stone deterioration process on the MNMs, although different lichen species were frequently observed on both the sandstones and the limestones. Hence, further investigation must be undertaken for determination of their long-term behavior and effects on the stones of the MNMs. On the other hand, the results of the FDA hydrolysis and plate count methods showed correlation. Lower bacterial counts were observed when lower enzymatic activity was observed in the stone samples, and likewise, higher bacterial counts were observed when higher enzymatic activity was observed. Consequently, the application of the FDA hydrolysis method was determined to be reliable for the estimation of total microbial activity on historical stones. The method had obvious advantages in terms of its rapid measurement rate and sensitivity, even on small samples.

Simultaneous determination of 8 fluoroquinolone antibiotics in sewage treatment plants by solid-phase extraction and liquid chromatography with fluorescence detection

2009 ◽  
Vol 59 (4) ◽  
pp. 805-813 ◽  
Author(s):  
L. Shi ◽  
X. F. Zhou ◽  
Y. L. Zhang ◽  
G. W. Gu
Keyword(s):  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Fluorescence Detection ◽  
Solid Phase ◽  
Sewage Treatment ◽  
Gradient Elution ◽  
Ammonium Bromide ◽  
Sewage Treatment Plants ◽  
Relative Standard

Fluoroquinolones (FQs) are among the most important antibiotics used in human and veterinary medicines. A simple and effective analytical method based on reversed-phased liquid chromatography with fluorescence was developed and validated for the simultaneous determination of eight FQs in wastewater at trace level. Aqueous samples were extracted using AnpelTM MEP cartridges where they were subsequently eluted by formic acid in methanol. The aqueous extracts were analyzed by gradient elution Liquid Chromatography with Fluorescence Detection (LC–FLD), whose mobile phase was composed of acetonitrile and 10 mM tetrabutyl ammonium bromide (TBAB). The limits of detection (LOD) and the relative standard deviation (RSD) were as low as 0.11–1.06 μg/L and 2–5%, respectively. The presented method was successfully applied to quantify FQs in the influent and effluent of several typical Sewage Treatment Plants (STPs) in Shanghai. For the extraction of 100 mL influent and 500 mL effluent sewage water samples, recoveries obtained were between 79–109% and 80%–105%, respectively. 7 FQs were occurred and identified in the STPs with the concentrations varying from 7 ng/L to 1 μg/L. Norfloxacin, ciprofloxacin and lomefloxacin were the most frequently detected antibiotics occurring in the wastewaters. The analytical procedure developed may be used for more in-depth studies on the occurrences and the fate of these commonly used pharmaceuticals in the sewage treatment plants and in the aquatic environment.

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