Assessment of a commercially available multiplex real-time PCR kit against direct immunofluorescence and nested PCRs for the detection of Giardia lamblia, Cryptosporidium spp. and Entamoeba histolytica in sewage

2017 ◽  
Vol 91 ◽  
pp. 101-111
Author(s):  
Constantine M. Vassalos ◽  
André Charlett ◽  
Evdokia Vassalou ◽  
Anastasia Mpimpa ◽  
George Dounias ◽  
...  
Keyword(s):  
Real Time ◽  
Entamoeba Histolytica ◽  
Real Time Pcr ◽  
Giardia Lamblia ◽  
Direct Immunofluorescence ◽  
Cryptosporidium Spp

MULTIPLEX REAL-TIME PCR ASSAY FOR DETECTION OF ENTAMOEBA HISTOLYTICA, GIARDIA INTESTINALIS, AND CRYPTOSPORIDIUM SPP.

2007 ◽  
Vol 76 (4) ◽  
pp. 713-717 ◽  
Author(s):  
RASHIDUL HAQUE ◽  
ERIC HOUPT ◽  
SHANTANU ROY ◽  
WILLIAM A. PETRI ◽  
S. M. MAZIDUR RAHMAN ◽  
...  
Keyword(s):  
Real Time ◽  
Entamoeba Histolytica ◽  
Real Time Pcr ◽  
Giardia Intestinalis ◽  
Pcr Assay ◽  
Cryptosporidium Spp

scholarly journals Chlamydia infection in patients with and without cervical intra-epithelial lesions tested by real-time PCR vs. direct immunofluorescence

2008 ◽  
Vol 12 (4) ◽  
pp. 324-328 ◽  
Author(s):  
Micheline de Lucena Oliveira ◽  
Melania Maria Ramos de Amorim ◽  
Paulo Roberto Eleutério de Souza ◽  
Lúcia Cristina Bezerra de Albuquerque ◽  
Lucas André Cavalcanti Brandão ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Chlamydia Infection ◽  
Direct Immunofluorescence ◽  
Epithelial Lesions

scholarly journals Cryptosporidium spp. during chemotherapy: a cross-sectional study of 94 patients with malignant solid tumor

2021 ◽  
Vol 41 (5) ◽  
pp. 293-298
Author(s):  
Mehmet Karabey ◽  
Hüseyin Can ◽  
Tülay Öncü Öner ◽  
Mert Döşkaya ◽  
Sedef Erkunt Alak ◽  
...  
Keyword(s):  
Sample Size ◽  
Real Time ◽  
Solid Tumors ◽  
Real Time Pcr ◽  
Tertiary Care ◽  
Diagnostic Methods ◽  
Cross Sectional ◽  
Cryptosporidium Spp ◽  
Stool Samples ◽  
Pcr Targeting

BACKGROUND: Cryptosporidium spp . is a protozoan parasite that infects many vertebrate animals, including humans. Since Cryptosporidium spp . can cause chronic life-threatening diarrhea and severe malabsorption in immunocompromised patients, we investigated the prevalence of this parasite among patients undergoing chemotherapy for malignant solid tumors. OBJECTIVE: Investigate the prevalence of Cryptosporidium spp . in stool samples. DESIGN: Cross-sectional. SETTING: Tertiary care. PATIENTS AND METHODS: Stool samples were collected from adult patients with malignant solid tumors receiving chemotherapy and diarrhea. Cryptosporidium spp . prevalence was determined using Ziehl–Neelsen staining, ELISA, and real-time PCR targeting of the COWP gene. MAIN OUTCOME MEASURE: The prevalence of Cryptosporidium spp . in patients undergoing chemotherapy for malignant solid tumors. SAMPLE SIZE: 94 RESULTS: The prevalence was 2.1% (2/94), 5.3% (5/94), and 5.3% (5/94) as detected by Ziehl–Neelsen staining, real-time PCR and ELISA, respectively. The prevalence reached 8.5% (8/94) using all results obtained from the three methods. Among eight positive stool samples, four were positive by at least two different methods (Ziehl–Neelsen staining-ELISA or ELISA-real-time PCR) whereas the remaining four were positive by either ELISA or real-time PCR. CONCLUSION: These findings show the risk of cryptosporidiosis in cancer patients and the necessity to use at least two diagnostic methods during the diagnosis of cryptosporidiosis to reach more accurate and trustworthy results. LIMITATIONS: Further studies with a larger sample size are recommended. CONFLICT OF INTEREST: None.

scholarly journals Genotype-specific detection of Giardia lamblia in stool samples of diarrhoeal and non-diarrhoeal patients in Dhaka, Bangladesh

1970 ◽  
Vol 20 (2) ◽  
pp. 183-189 ◽  
Author(s):  
Md Masud Alam ◽  
Mohammad Ilias ◽  
Md Abdullah Siddique ◽  
Md Mamun Kabir ◽  
Farida Nazib ◽  
...  
Keyword(s):  
Positive Result ◽  
Real Time ◽  
Real Time Pcr ◽  
Giardia Lamblia ◽  
Specific Antigen ◽  
Diarrhoeal Disease ◽  
Giardia Cyst ◽  
Positive Stool ◽  
Stool Samples ◽  
Assemblage B

Two major genotypic assemblages (A and B) of Giardia lamblia infect humans. A single-vessel multiplex real-time PCR assay was used that genotypes Giardia infections into assemblages A and/or B directly from fecal samples. In this study, 157 diarrhoeal (symptomatic) and non-diarrhoeal (asymptomatic) stool samples collected from the International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR,B) and Bangabandhu Sheikh Mujib Medical University (BSMMU) hospital, respectively were analyzed to determine whether an association exists between infections with G. lamblia assemblages A or B and diarrhea in Bangladesh. Of the 157 stool samples, Giardia cysts were observed in 35 by microscopy and 127 showed positive result for Giardia cyst specific antigen. The 127 ELISA positive samples were assayed for genotyping by real?time polymerase chain reaction. Of the 117 real-time PCR positive stool samples, 15 were positive for G. lamblia assemblage A, 96 were positive for assemblage B and 6 samples showed positive result for both G. lamblia assemblage A and B infections. Higher ratios for diarrhea were observed for assemblage A infections, whereas higher parasite DNA loads and a higher overall rate were observed for assemblage B infections in both diarrhoeal and non-diarrhoeal patients. Real-time PCR is, therefore, useful as an additional test supplementary to microscopy or enzyme immunoassay to detect genotypes of Giardia. Key words: Giardia lamblia; Genotypes; Multiplex real-time PCR; Immunoassay DOI: http://dx.doi.org/10.3329/dujbs.v20i2.8979 DUJBS 2011; 20(2): 183-189

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