scholarly journals Normal intraepithelial lymphocyte counts in duodenal biopsies and histological features of celiac disease among Jordanian adults

2021 ◽  
Vol 29 (1) ◽  
pp. 62-67
Author(s):  
Heyam A. Awad ◽  
Eman M. Krishan ◽  
Ahmad M. Abdulraheem ◽  
Mohammed M. Abu Jubba ◽  
Zaid S. Al-khateeb ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Intraepithelial Lymphocyte ◽  
Histological Features ◽  
Duodenal Biopsies

scholarly journals CORRELATION OF ENDOSCOPIC AND HISTOLOGICAL FEATURES IN ADULTS WITH SUSPECTED CELIAC DISEASE IN A REFERRAL CENTER OF MINAS GERAIS, BRAZIL

2014 ◽  
Vol 51 (4) ◽  
pp. 290-296 ◽  
Author(s):  
Rodrigo Macedo ROSA ◽  
Maria de Lourdes Abreu FERRARI ◽  
Moisés Salgado PEDROSA ◽  
Gustavo Meirelles RIBEIRO ◽  
Geraldo BRASILEIRO-FILHO ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Predictive Value ◽  
Villous Atrophy ◽  
Disease Diagnosis ◽  
Adult Patients ◽  
Intestinal Biopsy ◽  
Histological Features ◽  
Celiac Disease Diagnosis ◽  
Sensitivity Specificity ◽  
Duodenal Biopsies

Context Clinical presentation of celiac disease is extremely variable and the diagnosis relies on serologic tests, mucosal intestinal biopsy and clinic and serologic response to a gluten-free diet. Objectives To correlate the endoscopic and histological aspects of adult patients with suspicion of celiac disease and to evaluate the interobserver histological agreement. Methods Endoscopic aspects of 80 adult patients were evaluated and correlated with the histological features according the Marsh-Oberhuber classification system. The interobserver histological agreement was based on kappa values. Results The symptoms of the patients varied largely, with prominence for chronic diarrhea, present in 48 (60%) patients. The endoscopic aspects related with the duodenal villous atrophy had been observed in 32 (40%) patients. There were confirmed 46 cases of celiac disease, with prevalence of 57.5%. The sensitivity, specificity, positive predictive value and negative predictive value of the endoscopic markers for celiac disease diagnosis were of 60.9%, 88.2%, 87.5% and 62.5%. There was moderate interobserver histological agreement (kappa = 0.46). Conclusions The endoscopic markers of villous atrophy, although not diagnostic, had assisted in the suspicion and indication of the duodenal biopsies for diagnosis proposal. Histology is sometimes contradictory and new biopsies or opinion of another professional can provide greater diagnostic agreement.

scholarly journals Dietary Nanoparticles Interact with Gluten Peptides and Alter the Intestinal Homeostasis Increasing the Risk of Celiac Disease

2021 ◽  
Vol 22 (11) ◽  
pp. 6102
Author(s):  
Clara Mancuso ◽  
Francesca Re ◽  
Ilaria Rivolta ◽  
Luca Elli ◽  
Elisa Gnodi ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Metallic Nanoparticles ◽  
Synergistic Effects ◽  
Food Sector ◽  
Intestinal Homeostasis ◽  
Gluten Peptides ◽  
Transmission Electron ◽  
Duodenal Biopsies ◽  
Junction Proteins

The introduction of metallic nanoparticles (mNPs) into the diet is a matter of concern for human health. In particular, their effect on the gastrointestinal tract may potentially lead to the increased passage of gluten peptides and the activation of the immune response. In consequence, dietary mNPs could play a role in the increasing worldwide celiac disease (CeD) incidence. We evaluated the potential synergistic effects that peptic-tryptic-digested gliadin (PT) and the most-used food mNPs may induce on the intestinal mucosa. PT interaction with mNPs and their consequent aggregation was detected by transmission electron microscopy (TEM) analyses and UV–Vis spectra. In vitro experiments on Caco-2 cells proved the synergistic cytotoxic effect of PT and mNPs, as well as alterations in the monolayer integrity and tight junction proteins. Exposure of duodenal biopsies to gliadin plus mNPs triggered cytokine production, but only in CeD biopsies. These results suggest that mNPs used in the food sector may alter intestinal homeostasis, thus representing an additional environmental risk factor for the development of CeD.

scholarly journals Descriptive Study of the Different Tools Used to Evaluate the Adherence to a Gluten-Free Diet in Celiac Disease Patients

Nutrients ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 1777 ◽  
Author(s):  
Luis Rodrigo ◽  
Isabel Pérez-Martinez ◽  
Eugenia Lauret-Braña ◽  
Adolfo Suárez-González
Keyword(s):  
Celiac Disease ◽  
Multidisciplinary Approach ◽  
Descriptive Study ◽  
Duodenal Mucosa ◽  
Gluten Free Diet ◽  
Gluten Free ◽  
Autoimmune Process ◽  
Duodenal Biopsies

Celiac disease (CD) is a genetically conditioned autoimmune process that appears in susceptible people. It can affect people of any age, and slightly predominates in females. It has a fairly homogenous global distribution, with an average prevalence of 1–2%, the frequency having increased in recent decades. The only effective treatment is a strict and permanent gluten-free diet (GFD), although the level of compliance is poor, at about 50% of cases. To monitor the effectiveness of the GFD, several procedures involving various approaches are employed: (a) Periodic visits by expert Nutritionists; (b) Clinical follow-up; (c) Serological time controls of specific antibodies; (d) Serial endoscopies with collection of duodenal biopsies; (e) Use of structured questionnaires; and (f) Determination of gluten peptides derived from gluten in faeces and/or urine. All of these procedures are useful when applied, alone or in combination, depending on the cases. Some patients will only need to consult to their doctors, while others will require a multidisciplinary approach to assess their compliance with the GFD. In children, normalization of duodenal mucosa was achieved in 95% of cases within two years, while it is more delayed in adults, whose mucosa take longer time (3–5 years) to heal completely.

scholarly journals The Utility and Validation of Intraepithelial Lymphocyte Count in Duodenal Biopsies in A Tertiary Care Centre in South India

2019 ◽  
Vol 6 (10) ◽  
pp. A544-549
Author(s):  
Priyavadhana Balasubramanian ◽  
◽  
Bhawana Ashok Badhe ◽  
Rajesh Nachiappa Ganesh ◽  
Lakshmi C Panicker ◽  
...  
Keyword(s):  
Lymphocyte Count ◽  
South India ◽  
Tertiary Care ◽  
Intraepithelial Lymphocyte ◽  
Care Centre ◽  
Tertiary Care Centre ◽  
Duodenal Biopsies

BETA-GALACTOSIDASE AND BETA-GLUCURONIDASE ACTIVITIES IN INTESTINAL MUCOSA OF INFANTS AND CHILDREN

PEDIATRICS ◽  
1971 ◽  
Vol 47 (4) ◽  
pp. 737-744
Author(s):  
Irena Antonowicz ◽  
Harry Shwachman ◽  
Ishida Sotoo
Keyword(s):  
Celiac Disease ◽  
Intestinal Mucosa ◽  
Lysosomal Enzymes ◽  
Normal Activity ◽  
Acute Stage ◽  
Infants And Children ◽  
Protein Losing Enteropathy ◽  
Per Oral ◽  
Beta Galactosidase ◽  
Duodenal Biopsies

The activity of two galactosidases (lactase and hetero-β-galactosidase) and β-glucuronidase were studied in per oral duodenal biopsies in 50 infants and children. Ten patients served as controls and 40 had nutritional disorders including celiac disease (acute, and in remission), cystic fibrosis (CF), protein losing enteropathy, and some miscellaneous conditions. The values for the 10 control patients expressed in units/gm protein/minute ± S.D. follows: lactase 38.0 ± 13.4, H-β-gal-ase 1.42 ± 0.35., and β-glucuronidase 1.90 ± 0.45. In the acute stage of celiac disease the lactase values were markedly reduced, the H-β-gal-ase normal or slightly reduced, with normal activity for β-glucuronidase. In clinical remission and while still on a gluten-free diet the activity of lactase remained significantly reduced in seven of nine patients even after 2 to 10 years. The lysosomal enzymes H-β-gal-ase and β-glucuronidase were not strikingly affected in patients with CF although four of six patients showed low values for H-β-galactosidase. β-glucuronidase was not affected in a variety of intestinal disorders including those that severely affect the integrity of the intestinal mucosa. In the conditions studied there was no correlation between the activity of the two galactosidases, nor between the two lysosomal enzymes.

Are Complicated Forms of Celiac Disease Cryptic T-Cell Lymphomas?

Blood ◽  
1998 ◽  
Vol 92 (10) ◽  
pp. 3879-3886 ◽  
Author(s):  
Franck Carbonnel ◽  
Laurence Grollet-Bioul ◽  
Jean Claude Brouet ◽  
Marie Françoise Teilhac ◽  
Jacques Cosnes ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Celiac Disease ◽  
T Cell ◽  
Cell Lymphoma ◽  
Large Cell ◽  
Cell Receptor ◽  
T Cell Lymphoma ◽  
Gluten Free Diet ◽  
Gluten Free ◽  
Duodenal Biopsies

Abstract We assessed the clonality of duodenal mucosal T cells in patients with celiac disease and controls. Fifteen adult patients were studied. Four patients had a complicated celiac disease, 3 did not respond to a gluten-free diet, and 2 had an ulcerative jejunitis (including 1 patient with nonresponsive celiac disease). Seven patients had an untreated celiac disease responsive to a gluten-free diet. Histological examination of duodenal biopsies of these 11 patients showed benign-appearing celiac disease without evidence of lymphoma. Four patients with nonulcer dyspepsia and normal duodenal biopsies served as controls. TCRγ gene rearrangements were analyzed by multiplex polymerase chain reaction on DNA extracted from duodenal biopsies. Major clonal rearrangements of the T-cell receptor were found in 4 cases, all with complicated celiac disease. Monoclonality was confirmed by DNA sequencing of the junctional region in 3 cases and by hybridization with clone-specific oligoprobes. Patients with celiac disease responsive to gluten-free diet had mainly a polyclonal pattern, with 1 of them having an oligoclonal rearrangement. An oligoclonal pattern was also observed in 2 control patients. Three patients with complicated celiac disease evolved to T-cell lymphoma with liver (n = 2) or bone marrow (n = 1) invasion. Identical clones were found in the enteropathic duodenojejunum and peripheral blood in the patient with large-cell lymphoma with bone marrow invasion. This study suggests that complicated celiac disease is a cryptic T-cell lymphoma.

Discriminant Score for Celiac Disease Based on Immunohistochemical Analysis of Duodenal Biopsies

2015 ◽  
Vol 60 (5) ◽  
pp. 621-625 ◽  
Author(s):  
Antonella Tosco ◽  
Mariantonia Maglio ◽  
Francesco Paparo ◽  
Luigi Greco ◽  
Riccardo Troncone ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Immunohistochemical Analysis ◽  
Discriminant Score ◽  
Duodenal Biopsies
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