scholarly journals High performance liquid chromatography based characterization of Fengycin produced by Bacillus amyloliquefaciens against Fusarium graminearum and Rhizoctonia solani

2021 ◽  
Author(s):  
Muddasir Khan ◽  
◽  
Muhammad Salman ◽  
Abdullah Abdullah ◽  
◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Rhizoctonia Solani ◽  
Fusarium Graminearum ◽  
Bacillus Amyloliquefaciens ◽  
Crop Production ◽  
High Performance ◽  
Plant Diseases ◽  
Wide Range ◽  
Fungal Phytopathogens

A substantial loss of crop production worldwide is attributed to fungal phytopathogens. The most important economic pathogens among these fungal phytopathogens are Fusarium graminearum and Rhizoctonia solani, which cause a wide range of plant diseases. In the present study, Bacillus amyloliquefaciens secondary metabolite fengycin was identified by High performance liquid chromatography (HPLC) and in vitro screened against the Fusarium graminearum and Rhizoctonia solani. Based on the HPLC result, fengycin was identified at 215nm wavelength, retention time 5-7 minutes, and peak area was 3.914. The obtained results indicated that fengycin (1, 1/2, 1/4, and 1/8) concentrations have a significant effect (p<0.005) on the growth of F. graminearum and R. solani. The current study concluded that B. amyloliquefaciens secondary metabolites fengycin having a high potential to inhibit the growth of F. graminearum and R. solani.

scholarly journals Chemical Constituents of Snake Fruit (Salacca zalacca (Gaert.) Voss) Peel and in silico Anti-aging Analysis

2019 ◽  
Vol 3 (2) ◽  
pp. 122
Author(s):  
Ermi Girsang ◽  
I Nyoman Ehrich Lister ◽  
Chrismis Novalinda Ginting ◽  
Adrian Khu ◽  
Butter Samin ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Chlorogenic Acid ◽  
High Performance ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Palm Tree ◽  
Triterpenoid Saponin ◽  
Fruit Peel ◽  
Wide Range

Background: Skin aging is a condition where skin is unable to retain both its physiological and structural integrity. Plants is the main source of phtytochemicals compound with wide range of biological activities. Through the efforts of ongoing scientific researches, an increasing number of plant extracts and phytochemicals have been showed promising result as anti-aging agent. Snake fruit (Salacca zalacca (Gaert.) Voss) is tropical plant belongs to the palm tree family (Arecaceae) that served as important crop in Indonesia. Despite its utilization, the phytochemical compound available in snake fruit, especially its peel have not been well documented. Present study aimed to elucidate the phytochemical constituent of snake fruit peel and its anti-aging potency.Materials and Methods: Snake fruit peel extract (SPE) was subjected to qualitative phytochemical assay, high performance liquid chromatography, and molecular docking towards protein related in skin aging.Results: The screening showed SPE contained phytochemical compound belong to flavonoid, tannin, phenol, triterpenoid, saponin and alkaloid. Thus, based on the analysis only chlorogenic acid was present in SPE whilst rutin and caffeic acid were not detected. The SPE was contained chlorogenic acid around 1.074 mg/g dry weight. Chlorogenic acid had the high binding affinity towards matrix metalloproteinase (MMP)-1 (-9.4 kcal/mol).Conclusion: Current findings may provide scientific evidence for possible usage of SPE and its compounds as antioxidant and anti-aging agent.Keywords: Salacca zalacca, phytochemical compound, high performance liquid chromatography, anti-aging

Determination of Aflatoxins, Ochratoxin A, and Zearalenone in Mixed Feeds, with Detection by Thin Layer Chromatography or High Performance Liquid Chromatography

1981 ◽  
Vol 64 (6) ◽  
pp. 1356-1363 ◽  
Author(s):  
Mary V Howell ◽  
Philip W Taylor
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Limit Of Detection ◽  
Wide Range ◽  
Mixed Feeds ◽  
Layer Chromatography

Abstract A sensitive, reliable, and economical method for the determination of 6 mycotoxins in mixed feeds is described. The feed is extracted with chloroform-water and the extract is cleaned up by using a disposable Sep-Pak silica cartridge. The procedure requires less time (15 min from sample extraction to extract preparation) and less solvent (approximately one-tenth) compared with conventional methods and is suitable for a fast, economical screen. Additional cleanup procedures, involving dialysis or extraction into base, are described for samples containing high levels of interfering compounds. Thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) with fluorescence detection are described for identification and estimation of mycotoxins. The method has been applied to a wide range of mixed feeds, including laboratory animal diets, and raw materials. The limit of detection is 1 μg/kg for all mycotoxins measured by HPLC.

Vulcanization Chemistry. Sulfur, Nt-Butyl-2-Benzothiazole Sulfenamide Formulations Studied by High Performance Liquid Chromatography

1992 ◽  
Vol 65 (2) ◽  
pp. 488-501 ◽  
Author(s):  
A. B. Sullivan ◽  
Cynthia J. Hann ◽  
George H. Kuhls
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Material Balance ◽  
Side Reaction ◽  
Analytical Methodology ◽  
Wide Range ◽  
Kinetics Of ◽  
Vulcanization Process ◽  
Intermediate Concentration

Abstract The paper reports on the sulfur vulcanization chemistry of cis-polyisoprene formulations accelerated with N-t-butyl-2-benzothiazole sulfenamide. High Performance Liquid Chromatography (HPLC) is utilized to examine the kinetics of accelerator—sulfur disappearance and the formation—disappearance profiles of extra-network cure intermediates across the vulcanization process. Cure profile measurements are reported on three formulations covering a wide range of sulfur—accelerator ratios. Also unique aspects of the analytical methodology are highlighted. The observed cure-intermediate concentration profiles reinforce earlier theories about the chemical details of sulfurization and subsequent crosslinking. On all formulations, benzothiazole polysulfides (S1−S4) reach a maximum concentration at the scorch midpoint coincident with slope changes in reactant and product profiles. Although pendant-accelerator crosslink precursors are not measured directly, their presence is implied from material balance calculations. Also, the formation of a small amount of benzothiazole early in the scorch period is a new observation in sulfenamide acceleration. It suggests that accelerator hydrolysis is a competitive side reaction early in the vulcanization process.

Measurement of elemental sulfur in soil and sediments - Field sampling, sample storage, pretreatment, extraction and analysis by high performance liquid chromatography

Soil Research ◽  
1987 ◽  
Vol 25 (2) ◽  
pp. 167 ◽  
Author(s):  
JH Watkinson ◽  
A Lee ◽  
DR Lauren
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Elemental Sulfur ◽  
Soil Aggregates ◽  
Liquid Limit ◽  
Microwave Drying ◽  
Plastic Range ◽  
Moist Soil ◽  
Wide Range

A rapid method for measuring elemental sulfur (S0) in soil and sediments is described that is accurate and precise down to a detection limit of 0.1 mg kg-l. Elemental sulfur is extracted into chloroform and measured by high-performance liquid chromatography. For moist samples, water is added to exceed their liquid limit. The method has been used to follow oxidation of both natural and fertilizer So in soil in the laboratory and field. Sediments analysed included some from lakes, and settling ponds containing coal mine wastes. Extraction within the plastic range was incomplete and recoveries as low as 5%-65% were found for five soils selected for a wide range of plastic limit. The plastic soil aggregates were not dispersed in the water-immiscible chloroform, preventing the dissolution of occluded S0; but at moisture levels above and below the plastic range, the soil was dispersed and extraction of S0 was quantitative. Extraction into a water-miscible solvent, chloroform-methanol (50 : 50), dispersed the soil at all moistures by dissolving the water thus preventing formation of plastic soil. This was less convenient because either volume correction for moisture was needed, or excess water added later to separate the chloroform. Measurement of total sulfur (S) in chloroform by inductively coupled plasma gave comparable results where samples contained S0 fertilizer, but at the lower natural levels they were much less precise and sometimes higher because of the additional S compounds extracted. Storage of moist soil below -10�C or air-dry soil at 20�C, gave no loss of S0 after one month. Moist soil at 20�C gave 40% loss after one week, but at 4�C the loss was only 5% after four weeks. Forced air-drying at 30�C, freeze-drying and microwave drying (under a narrow range of conditions) gave losses of about 5% for fertilizer S0 (<0.15 mm fraction), and of about 20% for smaller S0 crystals. In field trials with S0, sample variability was associated mostly with the difficulty of applying the S0 uniformly and with the small number of particles applied per unit area. For particles <0.25 mm, variability from mixing of cores and sub-sampling was not so great.

scholarly journals Physical and Chemical Properties and Quality Control Methods of Hyaluronic Acid (Review)

2020 ◽  
Vol 9 (4) ◽  
pp. 136-140
Author(s):  
A. Kh. Amandusova ◽  
K. R. Savelyeva ◽  
A. V. Morozov ◽  
V. A. Shelekhova ◽  
V. N. Shestakov ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Performance Liquid Chromatography ◽  
Capillary Electrophoresis ◽  
Liquid Chromatography ◽  
Hyaluronic Acid ◽  
High Performance ◽  
Spectroscopic Method ◽  
Pharmaceutical Preparations ◽  
Turbidimetric Titration ◽  
Wide Range

Introduction. This review describes the physicochemical properties that determine the use of hyaluronic acid in ophthalmology. We have studied methods for determining hyaluronic acid using various analytical methods.Text. Hyaluronic acid is a high molecular weight glycosaminoglycan that consists of repeating disaccharides of N-acetylglucosamine and D-glucuronic acid. Carboxyl, hydroxyl and acetoamide groups give hydrophilic properties to the molecule of this anionic heteropolysaccharide. Depending on how the hyaluronic acid is obtained, its molecular weight varies over a wide range. Researchers developed methods for controlling hyaluronic acid, which include the turbidimetric titration method, the method of high-performance capillary electrophoresis and high-performance liquid chromatography and IR spectroscopic method.Conclusion. Due to its properties, hyaluronic acid is widely used as an active ingredient in pharmaceutical preparations. Today, there are a number of methods for the determination of hyaluronic acid, including the method of turbidimetric titration, the method of capillary electrophoresis. High performance liquid chromatography (HPLC) and IR spectroscopy methods are presented in the Japanese Pharmacopoeia and the European Pharmacopoeia. These techniques are widely used due to their high reproducibility, accuracy, and relative simplicity.

scholarly journals IDENTIFICATION OF FLAVONOIDS IN IRAQI HONEY AND COMPARISON WITH OTHERS KIND BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY WITH UV DETECTION

2017 ◽  
Vol 5 (4) ◽  
pp. 162-175
Author(s):  
Saadiyah A. Dhahir ◽  
Ameera H. Hamed
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Biological Effects ◽  
Natural Antioxidants ◽  
Reversed Phase ◽  
Test Range ◽  
Wide Range ◽  
Phase Column ◽  
Reversed Phase Column

Honey is rich in phenolic acids and flavonoids, which exhibit a wide range of biological effects and act as natural antioxidants. The analysis of polyphenols has been regarded as a very promising way of studying floral and geographical origins of honeys. The aim of this study was to determine, flavonoid in honey samples collected from different region in Iraq and Comparison with others kind. The flavonoids which measured in honey samples are (Myricetin, Quercetin, Hesperdin, Naringenin, Apigenin, Kaempferol, Chrisin). Chromatographic Separation was performed using a reversed phase column C18 and acetonitrile 60% (v/v) flow rate 1.0 ml/min, pH 5.5 at 280 nm. All calibration curves of the flavonoid compounds showed good linearity   (r -0.9999) within the test range. Concentration of flavonoids ranged from Myricetin (1.903- 18.5) mg/kg, Quercetin (3.31-22.01) mg/kg, Hesperdin (4.55-16.2) mg/kg, Naringenin (3.2-24.68) mg/kg, Apigenin (6.76-28.81) mg/kg, Kaempferol (3.31-38.13) mg/kg, Chrisin (2.0-28.0) mg/kg respectively.

Evaluation of intraocular penetration of levofloxacin by high performance liquid chromatography

2021 ◽  
Vol 2 (3) ◽  
pp. 155-158
Author(s):  
Mahsa Hasanzadeh ◽  
◽  
Amir Heydari ◽  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Cataract Surgery ◽  
High Performance ◽  
Topical Administration ◽  
Hplc Method ◽  
Fluorescence Detector ◽  
Wide Range ◽  
The Mean

AIM: To evaluate the levofloxacin eye drop into human eye penetration, levofloxacin eye drop concentrations in human ocular aqueous of 33 patients undergoing cataract surgery were measured by high performance liquid chromatography (HPLC). METHODS: Totally 33 volunteer patients who scheduled for phacoemulsification surgery received one drop of levofloxacin every 6h for 3d before and on the day of surgery, administration of drug was stopped 1h before surgery. Levofloxacin concentration in aqueous humor was measured by HPLC method with fluorescence detector. RESULTS: A simple, effective and sensitive HPLC method for determination of levofloxacin in human ocular aqueous was validated. Linearity was shown for levofloxacin concentration over a wide range of 1.95×10-3-1.50 µg/mL. The mean aqueous level of levofloxacin was 0.3399±0.03405 µg/mL. CONCLUSION: Results from the present study demonstrate that topical administration of levofloxacin 0.5% before cataract surgery with routine dose (one drop every 6h) unable to reach MIC90 for most common microorganism causing acute bacterial endophthalmitis.

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