scholarly journals A New and Improved Automated Technology for Early Sex Determination of Ginkgo biloba

2008 ◽  
Vol 34 (5) ◽  
pp. 300-307
Author(s):  
Vincent Echenard ◽  
François Lefort ◽  
Gautier Calmin ◽  
Robert Perroulaz ◽  
Lassaad Belhahri
Keyword(s):  
Sex Determination ◽  
Ginkgo Biloba ◽  
Dna Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Easy Method ◽  
Decamer Primer ◽  
Automated Technology ◽  
Sexual Cross ◽  
Male Specific

Random amplified polymorphic DNA (RAPD) technique with male associated decamer primer S1478 was used to amplify DNA from 72 leaf samples collected from Ginkgo biloba trees with known sexual determinism in the canton of Geneva, Switzerland. This marker was found to be male-specific and was lacking in all female plants. Automated random polymorphic DNA analysis (ARPA), a new automated technology developed in the frame of this work, proved highly effective in distinguishing males and females with 100% efficiency and successful in male and female discrimination from a collection of young seedlings derived from a sexual cross. Our findings provide unambiguous evidence that ARPA combined with the male-associated decamer primer S1478 could be considered an efficient, rapid, and easy method to make an early sex determination in the dioecious tree Ginkgo biloba.

A PCR assay for sex determination of yak (Bos grunniens) meat by amplification of the male-specific SRY gene

Food Control ◽  
2010 ◽  
Vol 21 (5) ◽  
pp. 726-731 ◽  
Author(s):  
W.L. Bai ◽  
R.H. Yin ◽  
S.J. Zhao ◽  
C. Li ◽  
Z.J. Ma ◽  
...  
Keyword(s):  
Sex Determination ◽  
Sry Gene ◽  
Pcr Assay ◽  
Bos Grunniens ◽  
Male Specific

scholarly journals Sex Determination of the Japanese Serow (Capricornis crispus) by Fecal DNA Analysis

2010 ◽  
Vol 15 (2) ◽  
pp. 73-78 ◽  
Author(s):  
Takashi NISHIMURA ◽  
Kiyoshi YAMAUCHI ◽  
Yasushi SAITOH ◽  
Yoshitaka DEGUCHI ◽  
Toshiki AOI ◽  
...  
Keyword(s):  
Sex Determination ◽  
Dna Analysis ◽  
Fecal Dna ◽  
Japanese Serow

scholarly journals Assessment of seed quality and sex determination in hop seedlings using molecular markers

2020 ◽  
Vol 42 ◽  
pp. e45
Author(s):  
Marília Pereira Machado ◽  
Andreza Cerioni Belniaki ◽  
André Felipe Bernert ◽  
Erik Nunes Gomes ◽  
João Carlos Bespalhok Filho ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Sex Determination ◽  
Seed Quality ◽  
Germination Percentage ◽  
Brewing Industry ◽  
Breeding Programs ◽  
Normal Seedling ◽  
Physiological Quality ◽  
Male Specific

Brazil is the world's third largest beer consumer and currently imports all of its hops for the brewing industry. Such a fact justifies the selection of hop genotypes adapted for cultivation locally, which requires high quality seeds and efficient sex determination of the seedlings. The objectives of this study were to develop a methodology to assess hop seed quality and to efficiently determine hop seedling sex through the use of male-specific molecular markers. Freshly harvested hop seeds were germinated with and without pre-chilling (3-5 ° C) for 3, 6 and 12 weeks and then germinated at 20 or 25 ° C in the presence or absence of light, evaluating germination percentage and germination speed index. F1 progenies were obtained from after seed germination in a greenhouse and seedlings sex was determined using male-specific molecular markers. The best conditions for physiological quality assessment of hop seeds used in the present study were pre-chilling for 12 weeks, followed by germination at 25 ° C, and normal seedling counts at 7 and 15 days. The progeny submitted to molecular marker sexing was composed of 61.3% female plants. The established methodologies presented here can be considered efficient and may contribute to expedite hops breeding programs.

Sex determination of bovine embryos using male-specific DNA probes

1988 ◽  
Vol 29 (1) ◽  
pp. 242 ◽  
Author(s):  
S.B. Ellis ◽  
K.R. Bondioli ◽  
M.E. Williams ◽  
J.H. Pryor ◽  
M.M. Harpold
Keyword(s):  
Sex Determination ◽  
Dna Probes ◽  
Bovine Embryos ◽  
Male Specific

scholarly journals Sex determination of skeletal remains of 4000 year old children and juveniles from Hoštice 1 za Hanou (Czech Republic) by ancient DNA analysis

2008 ◽  
Vol 71 (1) ◽  
pp. 63-70 ◽  
Author(s):  
Michaela Vaňharová ◽  
Eva Drozdová
Keyword(s):  
Czech Republic ◽  
Sex Determination ◽  
Ancient Dna ◽  
Dna Analysis ◽  
Molecular Genetic ◽  
Skeletal Remains ◽  
Burial Site ◽  
Ancient Dna Analysis ◽  
Bell Beaker

Sex determination of skeletal remains of 4000 year old children and juveniles from Hoštice 1 za Hanou (Czech Republic) by ancient DNA analysisThe aim of this study was to determine the sex by means of modern molecular genetic methods of children and immature individuals from the 4000 years old Eneolithic burial site "Hoštice 1 za Hanou" of the Bell-Beaker people, in central Moravia (Czech Republic). While the anthropological approach was in this case limited either by the state of preservation of the skeletal remains or simply by absence of definite morphological traits in the children, analysis of aDNA (SRY, amelogenin) yielded results consistent with archeological grave findings and body imposition. The burial rites of the investigated culture facilitated the analysis because the gender specific imposition of adults has previously been described (man left-side, head northwards, woman right-side, head southwards) However, this approach is often limited in case of children burials. This study showed high concordance between archeological sex-determination and genetic sex, but also revealed several exceptions in children burial rite of Bell Beaker culture.

scholarly journals RAPD Marker for Sex Determination of Dioecious Plants

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 878F-878 ◽  
Author(s):  
Deepak K. Khandka ◽  
Ali Nejidat ◽  
Moshe Tal ◽  
Avi Golan-Goldhirsh
Keyword(s):  
Bulked Segregant Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Sex Differentiation ◽  
Rapd Marker ◽  
Specific Marker ◽  
Dioecious Species ◽  
Important Species ◽  
Male Specific

Several horticulturally important species are dioecious (e.g., pistachio, date palm, poplar, and others). It would be advantageous if the gender of a seedling could be determined at the vegetative stage. In this report, we present results of our search for molecular markers for sex differentiation in dioecious species. The method used was bulked segregant analysis of random amplified polymorphic DNA (RAPD) for sex. A male-specific marker fragment OPB01-1470 was obtained in Mercurialis annua. The sex linkage and characterization of this marker will be discussed.

scholarly journals Morphological Characterisation And Molecular Sex Determination Of Human Remains From The 15th–17th Centuries In Latvia

2015 ◽  
Vol 69 (1-2) ◽  
pp. 8-13 ◽  
Author(s):  
Asta Ščėsnaitė-Jerdiakova ◽  
Liāna Pliss ◽  
Guntis Gerhards ◽  
Elīna Pētersone Gordina ◽  
Agnija Gustiņa ◽  
...  
Keyword(s):  
Sex Determination ◽  
Dna Analysis ◽  
Molecular Genetic ◽  
Skeletal Remains ◽  
Human Skeletal Remains ◽  
Full Agreement ◽  
Archaeological Material ◽  
Molecular Approaches ◽  
Molecular Sex Determination

Abstract Sex determination is one of the most important and initial steps in human profile identification from archaeological material. The aim of the current study was to evaluate the application of molecular approaches alongside morphological methods for sex determination in archaeological human skeletal remains. Human skeletal remains were excavated from three cemeteries: St Gertrude Old Church, Dom Square and St Peter’s Church, of 15th–17th century burials in Rīga, Latvia. Morphological and molecular genetic methods, including amplification of genes AMELX/Y and SRY were used to analyse seven skeletal remains. The conducted analyses of morphological features identified sex in all seven cases (two females and five males). By molecular analyses of mediaeval DNA it was possible to determine sex in five of seven (71%) samples. In all positive cases full agreement between morphological estimation and molecular genetic methods was observed. To conclude, DNA analysis can be considered for sex identification in cases with no signs of sexual dimorphism (juvenile skeletons) or partially preserved skeletons.

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