scholarly journals Degradation of aflatoxin in maize using Ferulic acid (phydroxy-3-methyl cinnamic acid) catalyzed by Hydrogen peroxide

2020 ◽  
Vol 1 (1) ◽  
pp. 1-17
Author(s):  
Nicholas M. Jacob ◽  
Shem O. Wandiga ◽  
David K. Kariuki ◽  
Vincent O. Madadi
Keyword(s):  
Hydrogen Peroxide ◽  
Reaction Time ◽  
Ferulic Acid ◽  
High Performance ◽  
Degradation Process ◽  
Hammer Mill ◽  
Fluorescence Detector ◽  
First Order Kinetics ◽  
Acid Catalyzed ◽  
Pre Treatment

Purpose: The study aimed to determine the rate of degradation of aflatoxin in contaminated maize using ferulic acid catalyzed by hydrogen peroxideMethodology: 100 g of dried maize grain was grounded using a laboratory hammer mill and divided into 2 portions of 50 g each. 20 g sample was taken per portion and treated with 100 mL solution of methanol and deionized water in the ration of 8:1, 50 mL of Acetonitrile, 1 g NaCl and 4 g of anhydrous magnesium sulphate, then blended at 120 RPM for 30 min. Aflatoxin content in each extract was analysed using enzyme-linked immunoassay test kits and confirmed using high performance liquid chromatography (HPLC) coupled with fluorescence detector. Further experiments tested the effect of coating, size, concentration, catalyst and reaction time on degradation of aflatoxin in maize. Data analysis was conducted using SPSS and Microsoft excel.Findings: Four-hour treatment of contaminated maize with 0.5 mM ferulic acid reduced aflatoxin by 91.0% for whole maize, 90.5% for dehulled maize and 90.9% for ground maize. Addition of 20 mL of 0.5% hydrogen peroxide to the reaction mixtures increased degradation of aflatoxin load to 99.0% for whole maize, 99.1% for dehulled maize and 99.1% for ground maize within 4-hour reaction time. The rate of decontamination followed first order kinetics with R2 values of 0.919, 0.916 and 0.930 for the whole maize, dehulled maize, ground maize, respectively and achieved degradation half-lives of 43.59, 41.26 and 39.84 minutes in the same order.Unique contribution to theory, practice and policy: Ferulic acid combined with hydrogen peroxide is an effective degrader of aflatoxin in maize. The rate degradation is dependent on the nature of maize pre-treatment, the concentration of ferulic acid, and the catalyst. Ferulic acid and hydrogen peroxide reacted with the lactone ring of the coumarin moity of aflatoxin. Recommendations; Further studies on degradation of aflatoxin in maize should elucidate the pathways and metabolites formed in the ferulic acid degradation process and determine their toxicities

scholarly journals ESTUDO DE ESTABILIDADE DO FOSFATO DISSÓDICO DE PREDNISOLONA EM CONDIÇÕES DE ESTRESSE OXIDATIVO E TÉRMICO, EM FORMULAÇÃO ORAL

2018 ◽  
Vol 35 (4) ◽  
pp. 09
Author(s):  
Cleber Antonio Lindino ◽  
Marcia Lina Mitsui ◽  
Rodolfo Ortiguara ◽  
Daiane Felin ◽  
Mauricio Ferreira Da Rosa ◽  
...  
Keyword(s):  
High Performance ◽  
Dosage Form ◽  
Degradation Products ◽  
Degradation Process ◽  
Oral Solution ◽  
Form Solution ◽  
Oral Dosage ◽  
First Order ◽  
First Order Kinetics

This work was to investigate the process of degradation of the drug Prednisolone Sodium Phosphate (FSP) in oral solution dosage form through the degradation experiments, evaluating the parameters in accordance with Resolution 899/2003 ANVISA and the degradation process of the drug. The method by high performance liquid chromatography (HPLC) developed for the determination of the drug was validated to demonstrate its applicability as an indicator of stability, ensuring reliability. After the method be validated to study the degradation of the drug, it was shown that drastic conditions of oxidative stress (H O 30%) and 2 2 temperature 60°C, the degradation of the drug is dependent on its concentration (first order kinetics). The results were  satisfactory, showing that this method is suitable to investigate the formation of degradation products in oral dosage form solution

scholarly journals Degradation Efficiency and Kinetics Analysis of an Advanced Oxidation Process Utilizing Ozone, Hydrogen Peroxide and Persulfate to Degrade the Dye Rhodamine B

Catalysts ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 974
Author(s):  
Piotr Zawadzki ◽  
Małgorzata Deska
Keyword(s):  
Hydrogen Peroxide ◽  
Rhodamine B ◽  
Advanced Oxidation Processes ◽  
Advanced Oxidation Process ◽  
Dye Degradation ◽  
Advanced Oxidation ◽  
Degradation Process ◽  
Oxidation Processes ◽  
First Order Kinetics ◽  
Ozonation Process

In this study, the effectiveness of a rhodamine B (RhB) dye degradation process at a concentration of 20 mg/L in different advanced oxidation processes—H2O2/UV, O3/UV and PDS/UV—has been studied. The use of UV in a photo-assisted ozonation process (O3/UV) proved to be the most effective method of RhB decolorization (90% after 30 min at dye concentration of 100 mg/L). The addition of sulfate radical precursors (sodium persulfate, PDS) to the reaction environment did not give satisfactory effects (17% after 30 min), compared to the PDS/UV system (70% after 30 min). No rhodamine B decolorization was observed using hydrogen peroxide as a sole reagent, whereas an effect on the degree of RhB degradation was observed when UV rays strike the sample with H2O2 (33% after 30 min). The rhodamine B degradation process followed the pseudo-first-order kinetics model. The combined PDS/O3/UV process has shown 60% color removal after 30 min of reaction time at an initial dye concentration of 100 mg/L. A similar effectiveness was obtained by only applying ozone or UV-activated persulfate, but at a concentration 2–5 times lower (20 mg/L). The results indicated that the combined PDS/O3/UV process is a promising method for high RhB concentrations (50–100 mg/L) comparing to other alternative advanced oxidation processes.

Сrude Plant Extracts Mediated Polyphenol Oxidation Reactions in the Presence of 3-Methyl-2-Benzothiazolinone Hydrazone for the Determination of Total Polyphenol Content in Beverages

2018 ◽  
Vol 15 (1) ◽  
pp. 11-20 ◽  
Author(s):  
Maria A. Morosanova ◽  
Anton S. Fedorov ◽  
Elena I. Morosanova
Keyword(s):  
Hydrogen Peroxide ◽  
Ferulic Acid ◽  
Gallic Acid ◽  
Caffeic Acid ◽  
Crude Extract ◽  
Reaction Rates ◽  
Green Bean ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Total Polyphenol Content

Background: The consumption of antioxidants, including phenolic compounds, is considered important for preventing the oxidative damage diseases and ageing. The total polyphenol content (TPC) is the parameter used to estimate the quality of plant-derived products. Methods: Phenol oxidase activity of green bean (Phaseolus vulgaris) crude extract (in the presence of hydrogen peroxide) and banana (Musa sp.) pulp crude extract has been studied spectrophotometrically using catechol, gallic acid, caffeic acid, ferulic acid, and quercetin as substrates. All studied compounds have been oxidized in the presence of green bean crude extract and hydrogen peroxide; all studied compounds except ferulic acid have been oxidized in the presence of banana pulp crude extract. Michaelis constants (Km) and maximum reaction rates (Vmax) have been determined for oxidation in the presence of green bean crude extract and hydrogen peroxide (Km are 3.8×10-4 M, 1.6×10-3 M, 2.2×10-4 M, 2.3×10-4 M, 1.4×10-4 M and Vmax are 0.046 min-1, 0.102 min-1, 0.185 min-1, 0.053 min-1, 0.041 min-1 for catechol, gallic acid, caffeic acid, ferulic acid, and quercetin, respectively) and for oxidation in the presence of banana pulp crude extract (Km are 1.6×10-3 M, 3.8×10-3 M, 2.2×10-3 M, 4.2×10-4 M and Vmax are 0.058 min-1, 0.025 min-1, 0.027 min-1, 0.015 min-1 for catechol, gallic acid, caffeic acid, and quercetin, respectively). The influence of 3-methyl-2-benzothiazolinone hydrazone (MBTH) on the oxidation reactions kinetics has been studied: Michaelis constants values decrease and maximum reaction rates increase, which contributes to the increase in sensitivity of the determination. Results: Kinetic procedures of Total Polyphenol Content (TPC) determination using crude plants extracts in the presence of MBTH have been proposed (time of analysis is 1 min). For gallic acid (used as a standard for TPC determination) detection limit is 5.3×10-5 M, quantitation limit is 1.8×10-4 M, and linear range is 1.8×10-4 - 1.3×10-3 M for green bean crude extract; detection limit is 2.9×10-5 M, quantitation limit is 9.5×10-5 M, and linear range is 9.5×10-5 - 2.4×10-3 M for banana pulp crude extract. Proposed procedures are characterized by higher interference thresholds for sulfites, ascorbic acid, and citric acid compared to pure enzymes (horseradish peroxidase and mushroom tyrosinase) in the same conditions. Compared with standard Folin-Ciocalteu (FC) method the procedures described in this work are also characterized by less interference and more rapid determination. Conclusion: The procedures have been applied to TPC determination in tea, coffee, and wine samples. The results agree with the FC method for tea and coffee samples and are lower for wine samples, probably, due to sulfites interference.

scholarly journals Highly-Efficient Release of Ferulic Acid from Agro-Industrial By-Products via Enzymatic Hydrolysis with Cellulose-Degrading Enzymes: Part I–The Superiority of Hydrolytic Enzymes Versus Conventional Hydrolysis

Foods ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 782
Author(s):  
Karina Juhnevica-Radenkova ◽  
Jorens Kviesis ◽  
Diego A. Moreno ◽  
Dalija Seglina ◽  
Fernando Vallejo ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Ferulic Acid ◽  
Hydrolytic Enzymes ◽  
Structural Complexity ◽  
Triticum Aestivum L ◽  
Feed Supplement ◽  
Degrading Enzymes ◽  
Secale Cereale L ◽  
Pre Treatment ◽  
By Products

Historically Triticum aestívum L. and Secale cereále L. are widely used in the production of bakery products. From the total volume of grain cultivated, roughly 85% is used for the manufacturing of flour, while the remaining part is discarded or utilized rather inefficiently. The limited value attached to bran is associated with their structural complexity, i.e., the presence of cellulose, hemicellulose, and lignin, which makes this material suitable mostly as a feed supplement, while in food production its use presents a challenge. To valorize these materials to food and pharmaceutical applications, additional pre-treatment is required. In the present study, an effective, sustainable, and eco-friendly approach to ferulic acid (FA) production was demonstrated through the biorefining process accomplished by non-starch polysaccharides degrading enzymes. Up to 11.3 and 8.6 g kg−1 of FA was released from rye and wheat bran upon 24 h enzymatic hydrolysis with multi-enzyme complex Viscozyme® L, respectively.

scholarly journals Hydrothermal Pretreatment of Wheat Straw: Effects of Temperature and Acidity on Byproduct Formation and Inhibition of Enzymatic Hydrolysis and Ethanolic Fermentation

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 487
Author(s):  
Dimitrios Ilanidis ◽  
Stefan Stagge ◽  
Leif J. Jönsson ◽  
Carlos Martín
Keyword(s):  
Mass Spectrometry ◽  
Sulfuric Acid ◽  
Wheat Straw ◽  
High Performance ◽  
Enzymatic Saccharification ◽  
Hydrothermal Pretreatment ◽  
Gas Chromatography Mass Spectrometry ◽  
Enzymatic Digestibility ◽  
Acid Catalyzed ◽  
Pretreatment Conditions

Biochemical conversion of wheat straw was investigated using hydrothermal pretreatment, enzymatic saccharification, and microbial fermentation. Pretreatment conditions that were compared included autocatalyzed hydrothermal pretreatment at 160, 175, 190, and 205 °C and sulfuric-acid-catalyzed hydrothermal pretreatment at 160 and 190 °C. The effects of using different pretreatment conditions were investigated with regard to (i) chemical composition and enzymatic digestibility of pretreated solids, (ii) carbohydrate composition of pretreatment liquids, (iii) inhibitory byproducts in pretreatment liquids, (iv) furfural in condensates, and (v) fermentability using yeast. The methods used included two-step analytical acid hydrolysis combined with high-performance anion-exchange chromatography (HPAEC), HPLC, ultra-high performance liquid chromatography-electrospray ionization-triple quadrupole-mass spectrometry (UHPLC-ESI-QqQ-MS), and pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS). Lignin recoveries in the range of 108–119% for autocatalyzed hydrothermal pretreatment at 205 °C and sulfuric-acid-catalyzed hydrothermal pretreatment were attributed to pseudolignin formation. Xylose concentration in the pretreatment liquid increased with temperature up to 190 °C and then decreased. Enzymatic digestibility was correlated with the removal of hemicelluloses, which was almost quantitative for the autocatalyzed hydrothermal pretreatment at 205 °C. Except for the pretreatment liquid from the autocatalyzed hydrothermal pretreatment at 205 °C, the inhibitory effects on Saccharomyces cerevisiae yeast were low. The highest combined yield of glucose and xylose was achieved for autocatalyzed hydrothermal pretreatment at 190 °C and the subsequent enzymatic saccharification that resulted in approximately 480 kg/ton (dry weight) raw wheat straw.

scholarly journals Polycyclic Aromatic Hydrocarbons Contamination of Flamed and Braised Chickens and Health Risk Assessment in Burkina Faso

Toxics ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 65
Author(s):  
Bazoin Sylvain Raoul Bazié ◽  
Caroline Douny ◽  
Thomas Judicaël Ouilly ◽  
Djidjoho Joseph Hounhouigan ◽  
Aly Savadogo ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Health Risk ◽  
Burkina Faso ◽  
Aromatic Hydrocarbons ◽  
High Performance ◽  
Maximum Level ◽  
Carcinogenic Risk ◽  
Daily Consumption ◽  
Fluorescence Detector ◽  
Polycyclic Aromatic

Charcoal- or wood-cooked chicken is a street-vended food in Burkina Faso. In this study, 15 samples of flamed chicken and 13 samples of braised chicken were analyzed for 15 priority polycyclic aromatic hydrocarbons (PAHs) with a high-performance liquid chromatography-fluorescence detector. A face-to-face survey was conducted to assess the consumption profiles of 300 men and 300 women. The health risk was assessed based on the margin of exposure (MOE) principle. BaP (14.95–1.75 μg/kg) and 4PAHs (BaP + Chr + BaA + BbF) (78.46–15.14 μg/kg) were eight and five times more abundant at the median level in flamed chickens than in braised ones, respectively. The contents of BaP and 4PAHs in all flamed chicken samples were above the limits set by the European Commission against 23% for both in braised chickens. Women had the highest maximum daily consumption of both braised (39.65 g/day) and flamed chickens (105.06 g/day). At the estimated maximum level of consumption, women were respectively 3.64 (flamed chicken) and 1.62 (braised chicken) times more exposed to BaP and 4PAHs than men. MOE values ranged between 8140 and 9591 for men and between 2232 and 2629 for women at the maximum level of consumption of flamed chickens, indicating a slight potential carcinogenic risk.

scholarly journals Selected Kefir Water from Malaysia Attenuates Hydrogen Peroxide-Induced Oxidative Stress by Upregulating Endogenous Antioxidant Levels in SH-SY5Y Neuroblastoma Cells

Antioxidants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 940
Author(s):  
Muganti Rajah Kumar ◽  
Swee Keong Yeap ◽  
Han Chung Lee ◽  
Nurul Elyani Mohamad ◽  
Muhammad Nazirul Mubin Aziz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Neuroblastoma Cells ◽  
Annexin V ◽  
Morphological Features ◽  
Lower Percentage ◽  
Total Phenolic ◽  
Neuroprotective Effects ◽  
Antioxidant Power ◽  
Pre Treatment

Kefir, a fermented probiotic drink was tested for its potential anti-oxidative, anti-apoptotic, and neuroprotective effects to attenuate cellular oxidative stress on human SH-SY5Y neuroblastoma cells. Here, the antioxidant potentials of the six different kefir water samples were analysed by total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant power (FRAP), and 2,2′-diphenyl-1-picrylhydrazyl radical (DPPH) assays, whereas the anti-apoptotic activity on hydrogen peroxide (H2O2) induced SH-SY5Y cells was examined using MTT, AO/PI double staining, and PI/Annexin V-FITC assays. The surface and internal morphological features of SH-SY5Y cells were studied using scanning and transmission electron microscopy. The results indicate that Kefir B showed the higher TPC (1.96 ± 0.54 µg GAE/µL), TFC (1.09 ± 0.02 µg CAT eq/µL), FRAP (19.68 ± 0.11 mM FRAP eq/50 µL), and DPPH (0.45 ± 0.06 mg/mL) activities compared to the other kefir samples. The MTT and PI/Annexin V-FITC assays showed that Kefir B pre-treatment at 10 mg/mL for 48 h resulted in greater cytoprotection (97.04%), and a significantly lower percentage of necrotic cells (7.79%), respectively. The Kefir B pre-treatment also resulted in greater protection to cytoplasmic and cytoskeleton inclusion, along with the conservation of the surface morphological features and the overall integrity of SH-SY5Y cells. Our findings indicate that the anti-oxidative, anti-apoptosis, and neuroprotective effects of kefir were mediated via the upregulation of SOD and catalase, as well as the modulation of apoptotic genes (Tp73, Bax, and Bcl-2).

A highly efficient multi-step methodology for the quantification of micro-(bio)plastics in sludge

2020 ◽  
pp. 0734242X2097409
Author(s):  
Federica Ruggero ◽  
Alexandra E. Porter ◽  
Nikolaos Voulvoulis ◽  
Emiliano Carretti ◽  
Tommaso Lotti ◽  
...  
Keyword(s):  
Organic Waste ◽  
Degradation Process ◽  
Organic Fertilizers ◽  
Critical Conditions ◽  
Organic Fraction ◽  
Initial Oxidation ◽  
Threshold Size ◽  
End Products ◽  
Biological Treatments ◽  
Pre Treatment

The present study develops a multi-step methodology for identification and quantification of microplastics and micro-bioplastics (together called in the current work micro-(bio)plastics) in sludge. In previous studies, different methods for the extraction of microplastics were devised for traditional plastics, while the current research tested the methodology on starch-based micro-bioplastics of 0.1–2 mm size. Compostable bioplastics are expected to enter the anaerobic or aerobic biological treatments that lead to end-products applicable in agriculture; some critical conditions of treatments (e.g. low temperature and moisture) can slow down the degradation process and be responsible for the presence of microplastics in the end-product. The methodology consists of an initial oxidation step, with hydrogen peroxide 35% concentrated to clear the sludge and remove the organic fraction, followed by a combination of flotation with sodium chloride and observation of the residues under a fluorescence microscope using a green filter. The workflow revealed an efficacy of removal from 94% to 100% and from 92% to 96% for plastic fragments, 0.5–2 mm and 0.1–0.5 mm size, respectively. The methodology was then applied to samples of food waste pulp harvested after a shredding pre-treatment in an anaerobic digestion (AD) plant in Italy, where polyethylene, starch-based Mater-Bi® and cellophane microplastics were recovered in amounts of 9 ± 1.3/10 g <2 mm and 4.8 ± 1.2/10 g ⩾2 mm. The study highlights the need to lower the threshold size for the quantification of plastics in organic fertilizers, which is currently set by legislations at 2 mm, by improving the background knowledge about the fate of the micro-(bio)plastics in biological treatments for the organic waste.

Formation of Samin Diastereomers by Acid-Catalyzed Transformation of Sesamolin with Hydrogen Peroxide

2020 ◽  
Vol 68 (23) ◽  
pp. 6430-6438
Author(s):  
Hsin-Ya Tsai ◽  
Wei-Ju Lee ◽  
I-Hsuan Chu ◽  
Wei-Ching Hung ◽  
Nan-Wei Su
Keyword(s):  
Hydrogen Peroxide ◽  
Acid Catalyzed
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