Analysis on Technical Difficulties and Quality Control Factors of Large Underground Space Construction

2021 ◽  
Keyword(s):  
Quality Control ◽  
Underground Space ◽  
Control Factors ◽  
Space Construction

scholarly journals POSSIBILITY OF UNDERGROUND SPACE CONSTRUCTION BY EXPLOSIVE LOADING IN A BOREHOLE

2004 ◽  
pp. 151-162
Author(s):  
Kazuyoshi TATEYAMA ◽  
Shingo HIRAI ◽  
Takao YANO ◽  
Toshihiro HENMI
Keyword(s):  
Explosive Loading ◽  
Underground Space ◽  
Space Construction

scholarly journals Chemical Biology Framework to Illuminate Proteostasis

2020 ◽  
Vol 89 (1) ◽  
pp. 529-555 ◽  
Author(s):  
Rebecca M. Sebastian ◽  
Matthew D. Shoulders
Keyword(s):  
Quality Control ◽  
Protein Folding ◽  
Chemical Biology ◽  
Control Factors ◽  
Network Composition ◽  
Network Component ◽  
Selection Of ◽  
In Cells

Protein folding in the cell is mediated by an extensive network of >1,000 chaperones, quality control factors, and trafficking mechanisms collectively termed the proteostasis network. While the components and organization of this network are generally well established, our understanding of how protein-folding problems are identified, how the network components integrate to successfully address challenges, and what types of biophysical issues each proteostasis network component is capable of addressing remains immature. We describe a chemical biology–informed framework for studying cellular proteostasis that relies on selection of interesting protein-folding problems and precise researcher control of proteostasis network composition and activities. By combining these methods with multifaceted strategies to monitor protein folding, degradation, trafficking, and aggregation in cells, researchers continue to rapidly generate new insights into cellular proteostasis.

scholarly journals Analysis of Geotechnical Engineering Safety Technology in Urban Underground Space Construction

2022 ◽  
Vol 6 (1) ◽  
pp. 1-6
Author(s):  
Yun Wan ◽  
Yaoping Luo
Keyword(s):  
Geotechnical Engineering ◽  
Underground Space ◽  
Safety Technology ◽  
Rational Application ◽  
Space Construction

With the accelerating pace of urbanization, the development and application of urban underground space has attracted much attention. In the construction of urban underground space, geotechnical engineering safety is the key point for construction. Based on this, this paper analyzes the application of geotechnical engineering safety technology in urban underground space construction, in hope that this analysis can provide a scientific reference for the rational application of geotechnical engineering safety technology as well as the construction and development of urban underground space.

scholarly journals Mutations Outside the Ure2 Amyloid-Forming Region Disrupt [URE3] Prion Propagation and Alter Interactions with Protein Quality Control Factors

2020 ◽  
Vol 40 (21) ◽  
Author(s):  
Shailesh Kumar ◽  
Elliot A. Dine ◽  
Ethan Paddock ◽  
Danielle N. Steinberg ◽  
Lois E. Greene ◽  
...  
Keyword(s):  
Quality Control ◽  
Protein Quality ◽  
De Novo ◽  
Protein Quality Control ◽  
Functional Domain ◽  
Amyloid Formation ◽  
Yeast Prions ◽  
Control Factors ◽  
Prion Propagation

ABSTRACT The yeast prion [URE3] propagates as a misfolded amyloid form of the Ure2 protein. Propagation of amyloid-based yeast prions requires protein quality control (PQC) factors, and altering PQC abundance or activity can cure cells of prions. Yeast antiprion systems composed of PQC factors act at normal abundance to restrict establishment of the majority of prion variants that arise de novo. While these systems are well described, how they or other PQC factors interact with prion proteins remains unclear. To gain insight into such interactions, we identified mutations outside the Ure2 prion-determining region that destabilize [URE3]. Despite residing in the functional domain, 16 of 17 mutants retained Ure2 activity. Four characterized mutations caused rapid loss of [URE3] yet allowed [URE3] to propagate under prion-selecting conditions. Two sensitized [URE3] to Btn2, Cur1, and Hsp42, but in different ways. Two others reduced amyloid formation in vitro. Of these, one impaired prion replication and the other apparently impaired transmission. Thus, widely dispersed sites outside a prion’s amyloid-forming region can contribute to prion character, and altering such sites can disrupt prion propagation by altering interactions with PQC factors.

Combinations of slow-translating codon clusters can increase mRNA half-life in Saccharomyces cerevisiae

2021 ◽  
Vol 118 (51) ◽  
pp. e2026362118
Author(s):  
Ajeet K. Sharma ◽  
Johannes Venezian ◽  
Ayala Shiber ◽  
Günter Kramer ◽  
Bernd Bukau ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Quality Control ◽  
Half Life ◽  
Synergistic Effects ◽  
Cellular Protein ◽  
Start Codon ◽  
Translational Efficiency ◽  
Control Factors ◽  
The Impact ◽  
Mrna Half Life

The presence of a single cluster of nonoptimal codons was found to decrease a transcript’s half-life through the interaction of the ribosome-associated quality control machinery with stalled ribosomes in Saccharomyces cerevisiae. The impact of multiple nonoptimal codon clusters on a transcript’s half-life, however, is unknown. Using a kinetic model, we predict that inserting a second nonoptimal cluster near the 5′ end can lead to synergistic effects that increase a messenger RNA’s (mRNA’s) half-life in S. cerevisiae. Specifically, the 5′ end cluster suppresses the formation of ribosome queues, reducing the interaction of ribosome-associated quality control factors with stalled ribosomes. We experimentally validate this prediction by introducing two nonoptimal clusters into three different genes and find that their mRNA half-life increases up to fourfold. The model also predicts that in the presence of two clusters, the cluster closest to the 5′ end is the primary determinant of mRNA half-life. These results suggest the “translational ramp,” in which nonoptimal codons are located near the start codon and increase translational efficiency, may have the additional biological benefit of allowing downstream slow-codon clusters to be present without decreasing mRNA half-life. These results indicate that codon usage bias plays a more nuanced role in controlling cellular protein levels than previously thought.

Progress in Research on Explosion Induced Thixotropy of Concrete

2011 ◽  
Vol 675-677 ◽  
pp. 643-646
Author(s):  
Peng Shao ◽  
Yu Yang Liu ◽  
Li Fen Zhou ◽  
Yu Qi Wu
Keyword(s):  
Steel Fibre ◽  
Fresh Concrete ◽  
Sol Gel ◽  
Future Research ◽  
Underground Space ◽  
Steel Fibre Reinforced Concrete ◽  
Space Construction ◽  
Thixotropic Behavior ◽  
General Meaning ◽  
Mechanical Performances

Thixotropy is an important property possessed by fresh concrete of changing from gel to liquid under shearing stress and returning to the original state when at rest. The thixotropic process is usually described to be an isothermal and static (or quasi-static) gel-sol-gel transformation. Now the thixotropy of concrete subjected to explosive loads became a new subject in research of synchronous explosive lining technology, which offers an advanced trechless method for underground space construction in soil. Because the detonation of explosives is a short-duration process accompanying with high temperature and high pressure, thixotropic behavior of concrete is no longer as same as that of in general meaning. This review summarizes the recent progress in research on explosion induced thixotropy of concrete, including the thixotropic behavior, thixoforming, influence of thixotropic process on microstructure, macrostructure and mechanical performances of concrete and steel fibre reinforced concrete. Additional, some arguments and suggestions for the future research are presented.

Note. Effect of a cryoprotectant agent (sodium tripolyphosphate) on hake slices preserved in modified atmosphere packaging/Nota. Efecto de un crioprotector (tripolyphosphate sódico) sobre merluza en rodajas envasada en atmósfera modificada

1996 ◽  
Vol 2 (3) ◽  
pp. 177-181 ◽  
Author(s):  
J.A. Alvarez ◽  
R. Pozo ◽  
L. Pastoriza
Keyword(s):  
Quality Control ◽  
Shelf Life ◽  
Sodium Tripolyphosphate ◽  
Modified Atmosphere Packaging ◽  
Modified Atmosphere ◽  
Chemical Parameters ◽  
Control Factors

Fresh hake slices packed in a modified atmosphere with a cryoprotectant agent (sodium tripolyphosphate) and under chilling conditions showed a reduction of exudate production. However, no improvement was achieved in microbiological and chemical parameters used as quality control factors compared with those packed only in a modified atmosphere (MAP). Thus final results concluded that the shelf-life of fresh hake slices packed using MAP and sodium tripolyphosphate was shorter than the shelf-life of fresh hake slices packed using only MAP.

scholarly journals EXPERIMENTAL STUDY ON UNDERGROUND SPACE CONSTRUCTION BY EXPLOSIVE LOADING OF BLASTING IN A BOREHOLE

2004 ◽  
pp. 175-183
Author(s):  
Kazuyoshi TATEYAMA ◽  
Toshihiro HENMI ◽  
Shingo HIRAI ◽  
Shigeru ITOH ◽  
Shiro NAGANO
Keyword(s):  
Experimental Study ◽  
Explosive Loading ◽  
Underground Space ◽  
Space Construction
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