Aseismic Design of Building Structure and Strengthening and Reconstruction Method

2021 ◽  
Keyword(s):  
Reconstruction Method ◽  
Building Structure ◽  
Aseismic Design

Three-dimensional reconstruction of the 40S ribosomal subunit from rabbit reticulocytes

1987 ◽  
Vol 45 ◽  
pp. 936-937
Author(s):  
Neng-Yu Zhang ◽  
Terence Wagenknecht ◽  
Michael Radermacher ◽  
Tom Obrig ◽  
Joachim Frank
Keyword(s):  
Three Dimensional ◽  
Ribosomal Subunit ◽  
Uranyl Acetate ◽  
Reconstruction Method ◽  
Single Exposure ◽  
Electron Dose ◽  
Ribosomal Subunits ◽  
40S Ribosomal Subunit ◽  
Dimensional Reconstruction ◽  
Rabbit Reticulocytes

We have reconstructed the 40S ribosomal subunit at a resolution of 4 nm using the single-exposure pseudo-conical reconstruction method of Radermacher et al.Small (40S) ribosomal subunits were Isolated from rabbit reticulocytes, applied to grids and negatively stained (0.5% uranyl acetate) in a manner that “sandwiches” the specimen between two layers of carbon. Regions of the grid exhibiting uniform and thick staining were identified and photographed twice (magnification 49,000X). The first micrograph was always taken with the specimen tilted by 50° and the second was of the Identical area untilted (Fig. 1). For each of the micrographs the specimen was subjected to an electron dose of 2000-3000 el/nm2.Three hundred thirty particles appearing in the L view (defined in [4]) were selected from both tilted- and untilted-specimen micrographs. The untilted particles were aligned and their rotational alignment produced the azimuthal angles of the tilted particles in the conical tilt series.

Development of Waveform Reconstruction Method with Current Transformer Saturation

2009 ◽  
Vol 129 (1) ◽  
pp. 15-21 ◽  
Author(s):  
Shoichi Urano ◽  
Takeshi Yamada ◽  
Yoshifumi Ooura ◽  
Youheng Xu ◽  
Yasutaka Yamaguchi ◽  
...  
Keyword(s):  
Current Transformer ◽  
Reconstruction Method

3D Scene Reconstruction Method in Relative Coordinates with Two Images from Non-Calibrated Video Cameras

2020 ◽  
Vol 25 (3) ◽  
pp. 265-276
Author(s):  
K.M. Shepilova ◽  
◽  
A.V. Sotnikov ◽  
A.V. Shipatov ◽  
Yu.V. Savchenko ◽  
...  
Keyword(s):  
Scene Reconstruction ◽  
Reconstruction Method ◽  
Video Cameras ◽  
Relative Coordinates ◽  
3D Scene ◽  
3D Scene Reconstruction
Sign in / Sign up

Export Citation Format

Share Document