A teacher’s journey from Japanese to TESOL to housewife to something else entirely

2020 ◽  
pp. 157-167
Author(s):  
Quenby Aoki
Keyword(s):  
Major Transition ◽  
Transition Points

Quenby Aoki explores various strands of her life, isolating the major transition points in the larger context of her teacher’s narrative. The author describes her transformation from professional to mother and “housewife” and her integration back into the life of an academic.

scholarly journals A multi-tiered map of EMT defines major transition points and identifies vulnerabilities

2021 ◽  
Author(s):  
Indranil Paul ◽  
Dante Bolzan ◽  
Heather Hook ◽  
Ahmed Youssef ◽  
Gopal Karemore ◽  
...  
Keyword(s):  
Epithelial To Mesenchymal Transition ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Tgfβ Signaling ◽  
Mesenchymal Transition ◽  
Whole Cells ◽  
Major Transition ◽  
Transition Points ◽  
Acid Pathway ◽  
Arachidonic Acid Pathway

TGFβ mediated epithelial to mesenchymal transition (EMT) proceeds through hybrid "E/M" states. A deeper understanding of these states and events which regulate entry to and exit from the E/M states is needed for therapeutic exploitation. We quantified >60,000 molecules across ten time points and twelve omic layers in mammary epithelial cells. Proteomes of whole cells, phosphoproteins, nucleus, extracellular vesicles, secretome and membrane resolved major shifts, E→E/M and E/M→M during EMT, and defined state-specific signatures. Metabolomics identified early activation of arachidonic acid pathway and an enzyme-mediated switch from Cytochrome P450 to Cyclooxygenase / Lipoxygenase branches during E→E/M. Single-cell transcriptomics identified GLIS2 as an early modulator of EMT. Integrative modeling-predicted combinatorial inhibition of AURKB, PP2A and SRC exposed vulnerabilities at E→E/M juncture. Covariance analysis revealed remarkable discordance between proteins and transcripts, and between proteomic layers, implying insufficiency of current approaches. Overall, this dataset provides an unprecedented resource on TGFβ signaling, EMT and cancer.

Survival of human epidermal keratinocytes after short-duration high temperature: synthesis of HSP70 and IL-8

1997 ◽  
Vol 272 (6) ◽  
pp. C1988-C1994 ◽  
Author(s):  
P. D. Bowman ◽  
S. T. Schuschereba ◽  
D. F. Lawlor ◽  
G. R. Gilligan ◽  
J. R. Mata ◽  
...  
Keyword(s):  
High Temperature ◽  
Cell Injury ◽  
Epidermal Keratinocytes ◽  
Human Epidermal Keratinocytes ◽  
Short Pulses ◽  
High Temperature Synthesis ◽  
Major Transition ◽  
Normal Human ◽  
Transition Points ◽  
Hsp70 Synthesis

Thermal injury by short pulses (1-30 s) of relatively high temperature (50-68 degrees C) was investigated in normal human epidermal keratinocytes (NHEK). NHEK were cultured on plastic cover-slips and dipped in medium held at various temperatures. Survival assessed by methylthiazol tetrazolium reduction assay at 6 days postheating demonstrated an inverse time-temperature relationship that indicated that most cells could survive after a 1-s, 60 degrees C exposure or a 30-s, 55 degrees C exposure. Arrhenius plots of the data indicated major transition points for cell injury at 50 and 60 degrees C. Heat shock protein 70 (HSP70) and interleukin-8 (IL-8) were both induced by elevation of temperature between 50 and 60 degrees C for as short a time as 1 s. HSP70 synthesis stimulated by short, high pulses of heat appeared to induce thermotolerance. These results demonstrate that brief exposure to relatively high temperature can induce HSP70 and IL-8 synthesis in keratinocytes.

The Major Transitions in Evolution

1997 ◽  
Author(s):  
John Maynard Smith ◽  
Eors Szathmary
Keyword(s):  
Full Range ◽  
Evolution Of Cooperation ◽  
Major Transitions ◽  
Insect Societies ◽  
Wide Range ◽  
Major Transition ◽  
Life Itself ◽  
History Of ◽  
Emergence Of Cooperation ◽  
Multicellular Organisms

Over the history of life there have been several major changes in the way genetic information is organized and transmitted from one generation to the next. These transitions include the origin of life itself, the first eukaryotic cells, reproduction by sexual means, the appearance of multicellular plants and animals, the emergence of cooperation and of animal societies, and the unique language ability of humans. This ambitious book provides the first unified discussion of the full range of these transitions. The authors highlight the similarities between different transitions--between the union of replicating molecules to form chromosomes and of cells to form multicellular organisms, for example--and show how understanding one transition sheds light on others. They trace a common theme throughout the history of evolution: after a major transition some entities lose the ability to replicate independently, becoming able to reproduce only as part of a larger whole. The authors investigate this pattern and why selection between entities at a lower level does not disrupt selection at more complex levels. Their explanation encompasses a compelling theory of the evolution of cooperation at all levels of complexity. Engagingly written and filled with numerous illustrations, this book can be read with enjoyment by anyone with an undergraduate training in biology. It is ideal for advanced discussion groups on evolution and includes accessible discussions of a wide range of topics, from molecular biology and linguistics to insect societies.

scholarly journals Biochemical and Initial Structural Characterization of the Monocot Chimeric Jacalin OsJAC1

2021 ◽  
Vol 22 (11) ◽  
pp. 5639
Author(s):  
Nikolai Huwa ◽  
Oliver H. Weiergräber ◽  
Christian Kirsch ◽  
Ulrich Schaffrath ◽  
Thomas Classen
Keyword(s):  
Physiological Role ◽  
Basic Function ◽  
Binding Activity ◽  
Carbohydrate Binding ◽  
Lectin Domain ◽  
Reducing Conditions ◽  
Transition Points ◽  
The Individual ◽  
High Yields ◽  
Dirigent Domain

The monocot chimeric jacalin OsJAC1 from Oryza sativa consists of a dirigent and a jacalin-related lectin domain. The corresponding gene is expressed in response to different abiotic and biotic stimuli. However, there is a lack of knowledge about the basic function of the individual domains and their contribution to the physiological role of the entire protein. In this study, we have established a heterologous expression in Escherichia coli with high yields for the full-length protein OsJAC1 as well as its individual domains. Our findings showed that the secondary structure of both domains is dominated by β-strand elements. Under reducing conditions, the native protein displayed clearly visible transition points of thermal unfolding at 59 and 85 °C, which could be attributed to the lectin and the dirigent domain, respectively. Our study identified a single carbohydrate-binding site for each domain with different specificities towards mannose and glucose (jacalin domain), and galactose moieties (dirigent domain), respectively. The recognition of different carbohydrates might explain the ability of OsJAC1 to respond to different abiotic and biotic factors. This is the first report of specific carbohydrate-binding activity of a DIR domain, shedding new light on its function in the context of this monocot chimeric jacalin.

scholarly journals An Investigation of the Cutting Strategy for the Machining of Polar Microstructures Used in Ultra-Precision Machining Optical Precision Measurement

Micromachines ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 755
Author(s):  
Chen-Yang Zhao ◽  
Chi-Fai Cheung ◽  
Wen-Peng Fu
Keyword(s):  
Precision Measurement ◽  
Detection Algorithm ◽  
Surface Generation ◽  
Precision Machining ◽  
Tool Geometry ◽  
Depth Of Cut ◽  
Machining Parameters ◽  
Ultra Precision ◽  
Transition Points ◽  
Cutting Strategy

In this paper, an investigation of cutting strategy is presented for the optimization of machining parameters in the ultra-precision machining of polar microstructures, which are used for optical precision measurement. The critical machining parameters affecting the surface generation and surface quality in the machining of polar microstructures are studied. Hence, the critical ranges of machining parameters have been determined through a series of cutting simulations, as well as cutting experiments. First of all, the influence of field of view (FOV) is investigated. After that, theoretical modeling of polar microstructures is built to generate the simulated surface topography of polar microstructures. A feature point detection algorithm is built for image processing of polar microstructures. Hence, an experimental investigation of the influence of cutting tool geometry, depth of cut, and groove spacing of polar microstructures was conducted. There are transition points from which the patterns of surface generation of polar microstructures vary with the machining parameters. The optimization of machining parameters and determination of the optimized cutting strategy are undertaken in the ultra-precision machining of polar microstructures.

scholarly journals Historical Ecology of Scandinavian Infield Systems

2021 ◽  
Vol 13 (2) ◽  
pp. 817
Author(s):  
Ove Eriksson ◽  
Matilda Arnell ◽  
Karl-Johan Lindholm
Keyword(s):  
Middle Ages ◽  
Iron Age ◽  
Historical Ecology ◽  
Niche Construction ◽  
Livestock Grazing ◽  
Land Reforms ◽  
Hay Meadows ◽  
Human Niche Construction ◽  
Major Transition ◽  
The 19Th Century

Infield systems originated during the early Iron Age and existed until the 19th century, although passing many transitions and changes. The core features of infield systems were enclosed infields with hay-meadows and crop fields, and unenclosed outland mainly used for livestock grazing. We examine the transitions and changes of domesticated landscapes with infield systems using the framework of human niche construction, focusing on reciprocal causation affecting change in both culture and environment. A first major transition occurred during the early Middle Ages, as a combined effect of a growing elite society and an increased availability of iron promoted expansion of villages with partly communal infields. A second major transition occurred during the 18th and 19th centuries, due to a then recognized inefficiency of agricultural production, leading to land reforms. In outlands, there was a continuous expansion of management throughout the whole period. Even though external factors had significant impacts as well, human niche construction affected a range of cultural and environmental features regarding the management and structure of domesticated landscapes with infield systems. Thus, niche construction theory is a useful framework for understanding the historical ecology of infield systems.

scholarly journals Detection of follicular regions in actin-stained whole slide images of the human lymph node by shock filter

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Patrick Wurzel ◽  
Jörg Ackermann ◽  
Hendrik Schäfer ◽  
Sonja Scharf ◽  
Martin-Leo Hansmann ◽  
...  
Keyword(s):  
Lymph Node ◽  
B Cells ◽  
Similarity Index ◽  
Morphological Differentiation ◽  
Development Stage ◽  
Immune Defense ◽  
Lymphoid Follicles ◽  
Shock Filter ◽  
Transition Points ◽  
Whole Slide Images

Abstract Human lymph nodes play a central part of immune defense against infection agents and tumor cells. Lymphoid follicles are compartments of the lymph node which are spherical, mainly filled with B cells. B cells are cellular components of the adaptive immune systems. In the course of a specific immune response, lymphoid follicles pass different morphological differentiation stages. The morphology and the spatial distribution of lymphoid follicles can be sometimes associated to a particular causative agent and development stage of a disease. We report our new approach for the automatic detection of follicular regions in histological whole slide images of tissue sections immuno-stained with actin. The method is divided in two phases: (1) shock filter-based detection of transition points and (2) segmentation of follicular regions. Follicular regions in 10 whole slide images were manually annotated by visual inspection, and sample surveys were conducted by an expert pathologist. The results of our method were validated by comparing with the manual annotation. On average, we could achieve a Zijbendos similarity index of 0.71, with a standard deviation of 0.07.

dc Conductivity Anomalies at the Transition Points in TTF–TCNQ

1976 ◽  
Vol 41 (1) ◽  
pp. 351-352 ◽  
Author(s):  
Takehiko Ishiguro ◽  
Seiichi Kagoshima ◽  
Hiroyuki Anzai
Keyword(s):  
Dc Conductivity ◽  
Conductivity Anomalies ◽  
Transition Points

Filtering of Markov renewal queues, IV: Flow processes in feedback queues

1985 ◽  
Vol 17 (2) ◽  
pp. 386-407 ◽  
Author(s):  
Jeffrey J. Hunter
Keyword(s):  
Queue Length ◽  
Queueing Systems ◽  
Arrival Process ◽  
Markov Renewal Process ◽  
Renewal Processes ◽  
Flow Process ◽  
Special Cases ◽  
Flow Processes ◽  
Transition Points ◽  
The Times

This paper is a continuation of the study of a class of queueing systems where the queue-length process embedded at basic transition points, which consist of ‘arrivals’, ‘departures’ and ‘feedbacks’, is a Markov renewal process (MRP). The filtering procedure of Çinlar (1969) was used in [12] to show that the queue length process embedded separately at ‘arrivals’, ‘departures’, ‘feedbacks’, ‘inputs’ (arrivals and feedbacks), ‘outputs’ (departures and feedbacks) and ‘external’ transitions (arrivals and departures) are also MRP. In this paper expressions for the elements of each Markov renewal kernel are derived, and thence expressions for the distribution of the times between transitions, under stationary conditions, are found for each of the above flow processes. In particular, it is shown that the inter-event distributions for the arrival process and the departure process are the same, with an equivalent result holding for inputs and outputs. Further, expressions for the stationary joint distributions of successive intervals between events in each flow process are derived and interconnections, using the concept of reversed Markov renewal processes, are explored. Conditions under which any of the flow processes are renewal processes or, more particularly, Poisson processes are also investigated. Special cases including, in particular, the M/M/1/N and M/M/1 model with instantaneous Bernoulli feedback, are examined.

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