Splat formulation for the control of Proeulia auraria (Lepidoptera: Tortricidae) through the method of sexual confusion in fruits

2019 ◽  
Vol 1 (1) ◽  
pp. 67-75
Author(s):  
Agustina Valverde-Rodriguez ◽  

The Proeulia auraria mating disruption (Lepidoptera: Tortricidae) using synthetic copies of the sex pheromone of the female insect, a mixture of two components E-tetradecyl acetate (E11-14: OAc) + E-11-tetradecanol (E11-14: OH), it was evaluated in orchards infested vine, apples and blueberries using the formulation SPLAT (Specialized Pheromone Lure Application and Technology). In terms of g pheromone and number of stations / sources 0.1 has been tested: 7.8 g in 25 sources, 7.8 g in 75 sources, and 29.5 g in 25 sources in vineyards; and 2.6 g in 25 sources (blueberry) and 75 sources (apple). All trials considered a witness without pheromone (control) and recorded weekly catches of adult male P. auraria Delta traps. In all treatments there was no P. auraria. Catches during the first three weeks, after application of SPLAT, compared with the control group (without pheromone), showing significantly higher catches. These results show a high percentage (100%) of disruption, however it must be confirmed in time and should be supplemented with assessments of damage to the foliage and fruit crops. Keywords: Pheromones, catches, Delta traps, mating disruption.

Insects ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 343
Author(s):  
Carolina Ballesteros ◽  
Alda Romero ◽  
María Colomba Castro ◽  
Sofía Miranda ◽  
Jan Bergmann ◽  
...  

Pseudococcus calceolariae, the citrophilous mealybug, is a species of economic importance. Mating disruption (MD) is a potential control tool. During 2017–2020, trials were conducted to evaluate the potential of P. calceolariae MD in an apple and a tangerine orchard. Two pheromone doses, 6.32 g/ha (2017–2018) and 9.45 g/ha (2019–2020), were tested. The intermediate season (2018–2019) was evaluated without pheromone renewal to study the persistence of the pheromone effect. Male captures in pheromone traps, mealybug population/plant, percentage of infested fruit at harvest and mating disruption index (MDI) were recorded regularly. In both orchards, in the first season, male captures were significantly lower in MD plots compared to control plots, with an MDI > 94% in the first month after pheromone deployment. During the second season, significantly lower male captures in MD plots were still observed, with an average MDI of 80%. At the third season, male captures were again significant lower in MD than control plots shortly after pheromone applications. In both orchards, population by visual inspection and infested fruits were very low, without differences between MD and control plots. These results show the potential use of mating disruption for the control of P. calceolariae.


Author(s):  
Asma Alonazi ◽  
Shahnaz Hasan ◽  
Shahnawaz Anwer ◽  
Azfar Jamal ◽  
Suhel Parvez ◽  
...  

This study compares the effects of electromyographic-biofeedback (EMG-BF)-guided isometric quadriceps strengthening with patellar taping and isometric exercise alone in patellofemoral pain syndrome (PFPS) among young adult male athletes. Sixty young adult male athletes with PFPS participated in the study. Participants were randomly divided into two groups: (1) EMG-BF-guided isometric exercise training with patellar taping (experimental group, n = 30), and (2) sham EMG-BF training with an isometric exercise program (control group, n = 30). Participants conducted their respective exercise programs for five days per week across four weeks. Study outcomes were pain (measured by the visual analog scale), functional disability (measured by the Kujala Anterior Knee Pain scale), and quadriceps strength (measured by an ISOMOVE dynamometer). Measurements were taken at baseline, Week 2, Week 4, and during a follow-up at Week 6. The experimental group demonstrated significantly lower VAS score at Weeks 2 and 4 compared to that of the control group (p = 0.008 and 0.0005, respectively). The score remained significantly lower at the Week 6 follow-up compared to the control group (p = 0.0005). There were no differences in knee function at Weeks 2 and 4 between the two groups (p = 0.086 and 0.171, respectively); however, the experimental group showed significantly better knee function at Week 6 compared to the control group (p = 0.002). There were no differences in quadriceps strength at Week 2 between the two groups (p = 0.259); however, the experimental group demonstrated significantly higher quadriceps strength at Weeks 4 and 6 compared to the control group (p = 0.0008). Four weeks of EMG-BF supplementation training with patellar taping demonstrated significant improvements in pain intensity, functional disability, and quadriceps muscle strength in young adult male athletes with PFPS.


1999 ◽  
Vol 131 (1) ◽  
pp. 137-149 ◽  
Author(s):  
R.M. Trimble ◽  
C.A. Tyndall ◽  
B.D. McGarvey

AbstractNatural rubber sleeve stoppers were impregnated with 10 mg of (E)-10-dodecen-1-yl-acetate, the major component of spotted tentiform leafminer, Phyllonorycter blancardella (F.), pheromone. In the laboratory, there was a linear decline in the amount of pheromone remaining on stoppers during 8 weeks of exposure to 10, 15, 20, and 25 °C. At 30 and 35 °C, the relationship was curvilinear and could be described using a second-order polynomial equation. The estimated rate of evaporation ranged from 0.03 mg/day at 10 °C to 0.08 mg/day at 25 °C; the rate of evaporation at 30 and 35 °C varied with the time since first exposure. There was a linear decline in the amount of pheromone remaining on stoppers during 8 weeks of exposure to fluctuating temperature regimes with average temperatures of 10 °C (i.e., 5–15 °C) and 20 °C (i.e., 15–25 °C). At a fluctuating regime with an average temperature of 30 °C (i.e., 25–35 °C), the relationship was curvilinear and could be described using a second-order polynomial equation. The estimated rate of evaporation was 0.02 and 0.09 mg/day at 5–15 and 15–25 °C, respectively; the estimated daily rate of evaporation at 25–35 °C varied with the time since first exposure. In an orchard, the estimated average rate of evaporation of pheromone from stoppers ranged from 0.05 to 0.31 mg/day and did not vary significantly with temperature. During the first 4 weeks of exposure in an orchard, the observed rate of evaporation was up to 4.4-fold greater than the rate predicted using the relationship between evaporation rate and constant temperatures observed in the laboratory. The potential for using natural rubber sleeve stoppers as controlled-release substrates in studies of sex-pheromone-mediated mating disruption of P. blancardella is discussed.


2017 ◽  
Vol 46 (4) ◽  
pp. 916-925
Author(s):  
Ri-zhao Chen ◽  
Chung-kuang Jow ◽  
Michael G. Klein ◽  
Yu-di Jia ◽  
Da-yu Zhang ◽  
...  

1990 ◽  
Vol 258 (6) ◽  
pp. E1033-E1036 ◽  
Author(s):  
L. C. Fernandes ◽  
U. F. Machado ◽  
C. R. Nogueira ◽  
A. R. Carpinelli ◽  
R. Curi

The effect of cachexia on insulin secretion was examined in adult male rats. Isolated islets of Langerhans from Walker 256 tumor-bearing rats secreted less insulin by glucose stimuli as compared with the control group; this was accompanied by significant change in 45Ca2+ outflow rate. Reduced insulin secretion to glucose stimuli in tumor-bearing rats probably led to low insulinemia (one-third). These findings indicate that reduced insulin secretion is probably an important factor for the development of cachexia in Walker 256 tumor-bearing rats.


Biologia ◽  
2014 ◽  
Vol 69 (10) ◽  
Author(s):  
Hana Ďúranová ◽  
Monika Martiniaková ◽  
Ivana Boboňová ◽  
Radoslav Omelka ◽  
Robert Stawarz ◽  
...  

AbstractCadmium (Cd) and diazinon (DZN) are known to be environmental risk factors for various bone diseases including osteoporosis. Selenium (Se), an essential constituent of many antioxidant enzymes, has in higher concentrations negative effects on the bone. The present study was aimed to investigate possible changes in femoral bone of adult male rats after their acute and subchronic exposures to Cd, DZN and Se. A total of 30 male Wistar rats were randomized into three experimental groups. The rats in the group A (4-months-old) were injected intraperitoneally with a mixture of 2 mg CdCl2 kg−1, 20 mg DZN kg−1 and 2 mg Na2SeO3 kg−1 body weight and killed 36 h after xenobiotics had been injected. In the group B, young males (1-month-old) were administered with a combination of 30 mg CdCl2 L−1, 40 mg DZN L−1 and 5 mg Na2SeO3 L−1 in their drinking water, for 90 days. Ten 4-months-old males without toxicant supplementation served as a control group (C). After treatment period, detailed histological analysis of femoral bone was performed in each group. Our results revealed apparent osteoporotic symptoms (resorption lacunae, osteoporotic fractures) in rats from groups A and B. Moreover, histomorphometrical evaluation showed reduced bone vascularization (constricted primary osteons’ vascular canals and Haversian canals) and weakness mechanical properties of bones (smaller size of the secondary osteons) in these rats in comparison with those of the control group. Our study demonstrates for the first time that acute and subchronic co-administrations to Cd, DZN and Se induce evident manifestation characteristics of osteoporosis in male rats.


1995 ◽  
Vol 268 (4) ◽  
pp. E546-E550 ◽  
Author(s):  
C. N. Boozer ◽  
G. Schoenbach ◽  
R. L. Atkinson

This study examined the effects of increasing levels of dietary fat fed isocalorically on body weight, body composition, and adipose distribution. Adult male rats were weight matched into four groups. One group that was fed a low-fat diet (12%) served as reference controls. The other three groups were fed diets of 24, 36, or 48% fat in amounts to equal the energy intake of the control group. After 6 wk, body weights of the four groups were not significantly different. Intrascapular brown fat did not differ between groups. Total body fat and adipose depot weights, however, increased in proportion to the level of fat in the diet. Total body fat and retroperitoneal and mesenteric depot weights of the 48% fat group were greater than controls (P < 0.05). Mesenteric fat in this group was also significantly increased over all other groups (P < 0.05). These results show that high-fat diets fed to adult animals cause increased body fat in the absence of significant changes in body weight and that mesenteric fat is increased disproportionately.


2014 ◽  
Vol 31 (02) ◽  
pp. 075-081
Author(s):  
A. Akinlolu ◽  
O. Akinola ◽  
P. Khobe ◽  
K. Obasi ◽  
O. Dada

Abstract Introduction: AAzathioprine and Methotrexate are both used in the treatment of cancer; and are classified as cytotoxic drugs with reported adverse effects such as oxidative damage to the DNA/RNA, the testes and sperm cells. This study, therefore, tested the hypothesis that AAzathioprine and Methotrexate administrations impair the morphology and functions of the testes in adult male wistar rats. Methods: AAzathioprine (50-150mg per day) and Methotrexate (2.5mg per week) are used in the treatment of cancer in adult Man. We tested the hypothesis that AAzathioprine and Methotrexate impair the morphology and functions of testes in rats. Forty adult male wistar rats (150-230g) were employed in the study: Control Group I received physiological saline while Experimental Groups II - V received oral administrations of 5mg/kg/bodyweight of AAzathioprine per day, 15mg/kg/bodyweight of AAzathioprine per day, 8mg/kg/bodyweight of Methotrexate per week and 20mg/kg/bodyweight of Methotrexate per week respectively for 35 days. Results: Histological examinations of the testes of rats of Groups II - V showed dose-dependent morphological anomalies such as fewer collagen ibers of connective tissues, disrupted seminiferous tubules and scanty spermatozoa when compared to rats of Group I. Statistical analyses showed dose-dependent elevated levels (P≤0.05) of superoxide dismutase and malondialdehyde in testes homogenates of rats of Groups II - V when compared to rats of Group I. This implied increased oxidative stress in rats of Groups II - V. Evaluations of Follicle Stimulating Hormone and Testosterone showed dose-dependent significantly elevated levels (P≤0.05) in rats of Groups II - V when compared to rats of Group I. Conclusions: Our findings are consistent with the stated hypothesis.


Author(s):  
Aalia Batool ◽  
Madiha Wazir ◽  
Rahim Ullah ◽  
Aalia Batool ◽  
Rabia Naz ◽  
...  

Stress represses hypothalamic-pituitary-gonadal axis (HPG-axis) but RF9, a synthetic peptide, rescues such repression. To assess the role of RF9 in regulating HPG-axis under normal physiological conditions in higher primates, RF9 was administered to intact adult male rhesus monkeys and response of the HPG-axis was examined by measuring plasma testosterone as an end parameter of the axis. Control group (n=4) received normal saline whereas the treated group (n=4) received RF9. On the first day of experiment, four bolus injections of normal saline (1ml/animal) were administered intravenously at 2-hr interval to the control monkeys. Similarly, on the second day of experiment, treated group received four iv bolus injections of RF9 (0.1mg/kg BW) at 2-hr interval. Serial blood samples were collected at 20 min interval during a 6-hr period which started just after first saline/RF9 injection. Plasma testosterone levels were measured by using a specific EIA. Overall means of plasma testosterone levels and plasma testosterone area under curve (AUC) and overall mean testosterone and mean testosterone AUC in short time windows following each injection of RF9 and saline were comparable between the groups. Our results demonstrate that RF9 has no role in regulating HPG-axis under normal physiological conditions in adult male monkeys.


QJM ◽  
2020 ◽  
Vol 113 (Supplement_1) ◽  
Author(s):  
M M Elshawwa

Abstract Background Obesity is associated with insulin resistance, type2 diabetes, dyslipidemia and cardiovascular diseases. Apelin and chemerin are identified as adipokines and adipose tissue markers. Several adipose-derived peptides are known to influence food intake, including apelin, whose expression is regulated by insulin and chemerin. Oxidative stress thought to be involved in the development of complications associated with obesity. Objective To study the nature of correlation between serum and liver levels of apelin, chemerin and oxidative parameters in obese rats with and without antioxidant. Aiming to clarify the pathophysiology of obesity. Material and Methods Thirty adult male albino rats, divided into three equal groups. Group I (control), group II (obese) and group III (obese and Lepidium sativum (LS) as an antioxidants). At the end of the experiment, blood samples were collected for estimation of the serum levels of chemerin, apelin, fasting glucose, insulin, insulin resistance (IR), lipid profile, reduced glutathione (GSH) and malondialdehyde (MDA). In addition to tissue homogenous extracts of liver were taken for the levels of MDA, CAT, chemerin and apelin. Results After eight weeks, high fat diet group showed a significant increase in serum levels of apelin, chemerin, fasting glucose, insulin, IR, total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C) & MDA and a significant decrease in high-density lipoprotein cholesterol (HDL-C) & GSH. HFD also caused a significant increase in tissue levels of MDA, CAT & chemerin and a significant decrease in apelin, compared to control group. While addition of LS to HFD caused a significant decrease in serum levels of apelin, chemerin, fasting glucose, insulin, IR, TC, TG, LDL-C & MDA and a significant increase in HDL-C & GSH. LS also caused a significant decrease in tissue levels of MDA, chemerin & insignificant decrease in CAT and a significant increase in apelin, compared to HFD group. Conclusion This study showed a significant positive correlation between liver & serum chemerin and between liver and serum MDA. On the other hand, it showed a significant negative correlation between liver and serum apelin and liver CAT and serum GSH


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