scholarly journals Lethal Antimicrobials and the Generality of Cellular Toxicity [University of Findlay]

2019 ◽  
Author(s):  
Zachary Wildman ◽  
Megan Pasternak ◽  
Robert Charvat ◽  
Justin Rheubert
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antimicrobial Properties ◽  
Therapeutic Index ◽  
Underlying Assumption ◽  
Cellular Toxicity ◽  
Snake Species ◽  
E Coli ◽  
Significant Difference ◽  
Antimicrobial Effectiveness

The exceedingly prevalent antibiotic resistance and need for effective antibiotic exploration has led researchers to a diverse array of biologically occurring compounds. In particular, snake venoms have antimicrobial properties and may be beneficial in medicinal development. Numerous studies have been completed on the Viperidae and Elapidae species, but only a handful of studies have been completed on the Colubridae. Although many bioactive components are found in colubrid venom, this dearth of studies may be due to the fact that many Colubridae species lack mammalian specific toxicity and an underlying assumption that antimicrobial activity is linked to general cellular toxicity of venom. This study addresses this assumption and tests hypotheses concerning the correlation between toxicity and antimicrobial effectiveness, in hopes of exploring less toxic venoms that possess antimicrobial properties. Antibacterial activity of snakes and LD50 values were obtained through an exhaustive literature search. Overall antibacterial activity of each snake species was calculated as a percent of effective/ineffective studies. A regression analysis was completed to test for correlation between LD50 and antibacterial effectiveness. Additionally, t-tests were performed to determine if LD50 values differed between effective/ineffective venoms. Preliminary results suggest that antimicrobial activity is not correlated to LD50 values. Furthermore, there is no significant difference in LD50 values between effective and ineffective venoms against the major bacteria tested (E. coli, S. aureus, P. aeruginosa, B. subtilis). These data reject the hypothesis that antimicrobial effectiveness is correlated to venom toxicity. Venom components, that are similar between snake species but lack mammalian cell toxicity may be the primary cause for venoms antimicrobial properties. Therefore, venom of a nontoxic colubrid potentially possesses antimicrobial properties, while being less toxic to patients; increasing the therapeutic index and lending support for further investigation.

scholarly journals Comparison of Antibacterial Activity ofLactobacillus plantarumStrains Isolated from Two Different Kinds of Regional Cheeses from Poland: Oscypek and Korycinski Cheese

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Aleksandra Ołdak ◽  
Dorota Zielińska ◽  
Anna Rzepkowska ◽  
Danuta Kołożyn-Krajewska
Keyword(s):  
Hydrogen Peroxide ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Broad Spectrum ◽  
Antimicrobial Properties ◽  
Antagonistic Activity ◽  
Antimicrobial Compounds ◽  
E Coli ◽  
Unpasteurized Milk ◽  
Protective Cultures

Oscypek and korycinski are traditional Polish cheeses, exclusively produced in Tatra and in Podlasie region, respectively, produced from raw, unpasteurized milk. The 29Lactobacillus plantarumstrains were isolated on MRS agar from 12 cheese samples and used as a material for study. The main purpose of the work was to assess the antimicrobial properties and recognition of selected strains for the unique antagonistic activity and preservation role in food. It has been found that the highest antimicrobial activity was observed in the case ofL. monocytogenesstrains; however, the level of that activity was different depending on theLb. plantarumstrain. Strains from oscypek produced broad spectrum, and a few strains isolated from korycinski cheese produced a narrow spectrum of antimicrobial compounds, other than organic acids and hydrogen peroxide. Moreover, the antagonistic activity shown byLb. plantarumstrains is connected with the source from which a given strain was isolated. Strains isolated from oscypek cheese represented stronger activity againstL. monocytogenes, whereas strains isolated from korycinski cheese were more active againstE. coli. StrainsLb. plantarumOs13 and Kor14 could be considered as good candidates for protective cultures to extend durability of food products.

scholarly journals Synthesis and Antimicrobial Activity of Metal-Containing Linseed Oil-Based Waterborne Urethane Oil Wood Coatings

Polymers ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 663 ◽  
Author(s):  
Kun-Tsung Lu ◽  
Jing-Ping Chang
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antimicrobial Agents ◽  
Linseed Oil ◽  
White Rot ◽  
White Rot Fungus ◽  
Manganese Acetate ◽  
Film Properties ◽  
E Coli ◽  
Significant Difference

In this study, the antimicrobial agents of mono(hydroxyethoxyethyl)phthalate (M(HEEP)2) with different metal of M = Zn, Mn, Pb, and Ca were synthesized from diethylene glycol (DEG), phthalic anhydride (PA), and divalent metal acetates including calcium acetate, zinc acetate, manganese acetate, and lead acetate, respectively. The waterborne urethane oil (WUO) dispersions synthesized from linseed oil, diisocyanates (hexamethylene diisocyanate (HDI) and isophorone diisocyanate (IPDI)), dimethylolpropionic acid at NCO/OH molars of 0.9, by acetone processing method were described as in our previous report. The M(HEEP)2 antimicrobial agents as well as the commercial nanosilver powder were added into WUO dispersions as the antimicrobial coatings. The effects of various antimicrobial agents and dosages (0.0, 0.2, 0.6, 0.8, 1.0, 2.0, and 4.0 phr) on antimicrobial activity of WUO films against gram-negative bacterium of Escherichia coli, gram-positive bacterium of Staphylococcus aureus, brown-rot fungus of Gloeophyllum trabeum, and white-rot fungus of Lenzites betulina were assessed. In addition, the film properties of the best antimicrobial WUO coatings were also examined. The results showed that the antimicrobial agents of mono(hydroxyethoxyethyl) phthalate M(HEEP)2 (M = Zn, Mn, Pb, and Ca) powders should certainly be synthesized by FTIR, 1H-NMR, 13C-NMR, and energy-dispersive X-ray spectroscopy (EDS) identifications and the yields of them were 43–55%. The results also revealed that the WUO film synthesizing with HDI films containing Zn(HEEP)2 of 2.0 phr and Pb(HEEP)2 of 0.4 phr had the best antibacterial activity for E. coli and S. aureus, respectively. The IPDI films containing Zn(HEEP)2 of 1.0 phr had the best antibacterial activity for both E. coli and S. aureus. For antifungal activity, the WUO film synthesizing with HDI films containing Pb(HEEP)2 of 0.8 phr and Zn(HEEP)2 of 2.0 phr as well as IPDI films containing Mn(HEEP)2 of 0.2 phr and Zn(HEEP)2 of 4.0 phr had the best performances against G. trabeum and L. betulina, respectively. Comparing with commercial nanoAg powder, the Zn(HEEP)2 and Pb(HEEP)2 had a superior antifungal efficiency for G. trabeum and L. betulina, while it had a slightly inferior efficiency in the antibacterial activity for E. coli and S. aureus. On the properties of WUO films, adding metal-containing antimicrobial agents could slightly enhance the thermal stability, but lowered the gloss of all films, however, the Tg value increased for HDI film and decreased for IPDI film. In addition to this, they had no significant difference in the film properties including hardness, impact resistance, bending resistance, adhesion, mass retention, and light-fastness between the WUO films with and without adding antimicrobial agents.

Disinfection of Fruits with Activated Water

2019 ◽  
Vol 10 ◽  
pp. 1864-1872
Author(s):  
Prof. Teodora P. Popova
Keyword(s):  
Antimicrobial Activity ◽  
Aqueous Solutions ◽  
Antimicrobial Properties ◽  
The Other ◽  
Gram Negative Bacteria ◽  
High Antimicrobial Activity ◽  
Gram Negative ◽  
E Coli ◽  
Number Of Microorganisms ◽  
Lower Effect

The effect of ionized aqueous solutions (anolytes and catholyte) in the processing of fruits (cherries, morellos, and strawberries) for decontamination has been tested. Freshly prepared analytes and catholyte without the addition of salts were used, as well as stored for 7 months anolytes, prepared with 0.5% NaCl and a combination of 0.5% NaCl and 0.5% Na2CO3. The anolyte prepared with a combination of 0.5% NaCl and 0.5% Na2CO3, as well as the anolyte obtained with 0.5% NaCl, exhibit high antimicrobial activity against the surface microflora of strawberries, cherries, and sour cherries. They inactivate E. coli for 15 minutes. The other species of the fam. Enterobacteriaceae were also affected to the maximum extent, as is the total number of microorganisms, especially in cherries and sour cherries. Even stored for 7 months, they largely retain their antimicrobial properties. Anolyte and catholyte, obtained without the addition of salts, showed a lower effect on the total number of microorganisms, but had a significant effect on Gram-negative bacteria, and especially with regard to the sanitary indicative E. coli.

scholarly journals An In Vitro Study on the Antimicrobial Properties of Essential Oil Modified Resin Composite against Oral Pathogens

Materials ◽  
2020 ◽  
Vol 13 (19) ◽  
pp. 4383
Author(s):  
Barbara Lapinska ◽  
Aleksandra Szram ◽  
Beata Zarzycka ◽  
Janina Grzegorczyk ◽  
Louis Hardan ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Antimicrobial Agents ◽  
Resin Composite ◽  
Antimicrobial Properties ◽  
In Vitro Study ◽  
Diffusion Method ◽  
Oral Pathogens

Modifying the composition of dental restorative materials with antimicrobial agents might induce their antibacterial potential against cariogenic bacteria, e.g., S.mutans and L.acidophilus, as well as antifungal effect on C.albicans that are major oral pathogens. Essential oils (EOs) are widely known for antimicrobial activity and are successfully used in dental industry. The study aimed at evaluating antibacterial and antifungal activity of EOs and composite resin material (CR) modified with EO against oral pathogens. Ten EOs (i.e., anise, cinnamon, citronella, clove, geranium, lavender, limette, mint, rosemary thyme) were tested using agar diffusion method. Cinnamon and thyme EOs showed significantly highest antibacterial activity against S.mutans and L.acidophilus among all tested EOs. Anise and limette EOs showed no antibacterial activity against S.mutans. All tested EOs exhibited antifungal activity against C.albicans, whereas cinnamon EO showed significantly highest and limette EO significantly lowest activity. Next, 1, 2 or 5 µL of cinnamon EO was introduced into 2 g of CR and microbiologically tested. The modified CR showed higher antimicrobial activity in comparison to unmodified one. CR containing 2 µL of EO showed the best antimicrobial properties against S.mutans and C.albicans, while CR modified with 1 µL of EO showed the best antimicrobial properties against L.acidophilus.

scholarly journals In vitro evaluation of the antimicrobial activity of five root canal sealers

2004 ◽  
Vol 15 (1) ◽  
pp. 30-35 ◽  
Author(s):  
Brenda Paula Figueiredo de Almeida Gomes ◽  
José Assis Pedroso ◽  
Rogério Castilho Jacinto ◽  
Morgana Eli Vianna ◽  
Caio Cezar Randi Ferraz ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Properties ◽  
Contact Method ◽  
Streptococcus Sanguis ◽  
Actinomyces Naeslundii ◽  
Agar Diffusion Test ◽  
Significant Difference ◽  
Ah Plus ◽  
Endodontic Sealers

The aim of the present study was to analyze the antimicrobial properties of five endodontic sealers: Endo Fill, Endomethasone, Endomethasone N, Sealer 26 and AH-Plus, against the following microorganisms: Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus sanguis and Actinomyces naeslundii. The sealers were tested immediately, 24 h, 48 h and 7 days after manipulation.The direct contact method through the observation of the microbial growth in liquid medium and the agar diffusion test were used to evaluate the antimicrobial properties of the sealers. The results, in both methodologies used, showed that immediately after manipulation, Endo-Fill and Endomethasone demonstrated the highest antimicrobial activity, with no statistically significant difference between them. Sealer 26 demonstrated the lowest antimicrobial activity. At all other times after manipulation, there were no statistically significant differences among all the sealers tested. In conclusion, none of the sealers totally inhibited the growth of the microorganisms. Furthermore, the antimicrobial activity of each sealer decreased with time and was dependent upon the microbial susceptibility to them.

scholarly journals Antibacterial activity of ginger extracts on bacteria isolated from digestive tract infection patients attended Muhoza Health Center

2020 ◽  
Vol 11 (2) ◽  
pp. 35-41
Author(s):  
Callixte Yadufashije ◽  
Adolyne Niyonkuru ◽  
Emanuel Munyeshyaka ◽  
Sibomana Madjidi ◽  
Joseph Mucumbitsi
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Tract Infection ◽  
Health Center ◽  
Digestive Tract ◽  
Inhibition Zone ◽  
Ginger Extract ◽  
E Coli ◽  
Shigella Spp ◽  
Stool Samples

Background: Ginger (Zingiber officinale) has been used for long time due to its potential antimicrobial activity against diversity of microbial pathogens. Aims and Objectives: The study was carried out to investigate the bacteria pathogens found in digestive tract infections and assess antimicrobial activities of ginger extract to identified bacteria. Materials and Methods: Bacteriological studies were carried out on stool samples from 30 patients attending Muhoza health center. Different types of bacteria were isolated from stool samples of digestive tract infection patients by using various methods such culture, biochemical test and antimicrobial activity of ginger extracts was analyzed at INES-Ruhengeri in clinical microbiology laboratory. Results: Study findings showed isolated bacteria and antibacterial activity of Ginger. Isolated bacteria and their percentages including Escherichia coli (46.6%) which is the predominant isolated bacteria, Salmonella species (33.33%), Enterobacter spp (10.0%), Shigella spp (6.6%) and Citrobacter (3.33%) which is the least isolated bacteria. Antibacterial activity of ginger was seen on isolated bacteria, as ethanol and methanol were used for ginger oil extraction, the antibacterial activity of ginger extracts using ethanol was seen on isolated bacteria such us Citrobacter spp with 14 mm of inhibition zone, Shigella spp with 12 mm, Salmonella with 11.1 mm, E. coli with 9.5 mm and Enterobacter spp which was seen to be resistant to ginger extract using ethanol with 0.66mm of inhibition zone. For methanol extracts antibacterial activity was seen as follows: Citrobacter spp at 12 mm, Shigella spp at 11 mm, E. coli at 8 mm, salmonella spp at 6.1 mm, and Enterobacter spp with 5 mm. Enterobacter spp was seen to be the most resistant bacteria in both extracts. Conclusion: Ginger has shown to have an antibacterial activity on bacteria isolated from digestive tract infected patients. It can be used as a medicine to treat these infections. Number of researches should be done to be sure on this reality of antibacterial activity of ginger.

scholarly journals Justification of sorption composite usage on the basis of nanodisperse silica for sepsis prevention in complex treatment of purulent-inflammatory processes

2019 ◽  
Vol 41 part 3 (2) ◽  
pp. 45-50
Author(s):  
O. A. Viltsaniuk ◽  
P. V. Belyaev ◽  
T. P. Osolodchenko ◽  
O. O. Viltsaniuk
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Properties ◽  
Statistical Processing ◽  
Toxic Substances ◽  
Adhesive Properties ◽  
Adhesion Properties ◽  
E Coli ◽  
Adhesion Ability ◽  
Treatment And Prevention ◽  
Inflammatory Processes

Introduction. The problem of purulent-inflammatory processes treatment and the prevention of sepsis remains one of the most pressing problems of surgery.Purpose of the study. Is to estimate the effectiveness of sorption composition with antimicrobial properties on the basis of nanodisperse silica on the adhesive properties of microorganisms and to substantiate the expediency of its use for the purulent and inflammatory processes treatment and prevention of sepsis.Materials and methods.To determine the antimicrobial activity and the influence of the antiseptics of decasan, myramistin, ethonium, chlorhexidine and the sorption on the microorganisms adhesion properties composition was used the method of diffusion in agar of the museum strains of microorganisms S. Aureus ATCC 25923, E. Coli ATCC 25922, R. Vulgaris 4636, R. Aureginosa ATCC 27853, S. AIbicans 855/653, SI. Perfringens 28, Peptostreptococcus anaerobicus 22, Bacteroides fragilis 13/83, and clinical strains of S. Aureus, R. Aeuroginosa, E. Coli. Adhesive properties of microorganisms were studied according to the Brillis method et al. (1986) with the subsequent statistical processing of the data.Results. Studies have shown that antiseptics have high antimicrobial activity and reduce the adhesion ability of microorganisms. But the use of only antiseptics or only antibiotics for the purification of purulent foci can lead to toxins absorption and tissue decay products from purulent foci. That processes could be the trigger mechanism for sepsis development. The sorption composition is able to reduce the adhesive activity of bacteria, allows to absorb toxic substances from pathological foci, what preventing their resorption and thereby prevent the development of sepsis.Conclusions. The composition made from sorbents and antiseptics is not inferior to antimicrobial activity to antiseptics, which can reduce the virulence of bacteria, by reducing their adhesive activity, and absorb toxins of microorganisms and tissue decomposition products on their surface, thus prevent their absorption, which will prevent the purulent-inflammatory process progression and sepsis development.Keywords: sorption composition, nanodisperse silica, antiseptics, adhesive properties of microorganisms, prevention of sepsis.

Exposure-Dependent Antimicrobial Activity and Oxidative Properties of Polymer-Based Graphene Oxide Nanocomposites

2019 ◽  
Vol 947 ◽  
pp. 13-20
Author(s):  
Jem Valerie D. Perez ◽  
Joy Vanessa D. Perez ◽  
Raniv D. Rojo ◽  
Maria Lourdes P. Dalida ◽  
Debora F. Rodrigues
Keyword(s):  
Antimicrobial Activity ◽  
Graphene Oxide ◽  
Membrane Damage ◽  
Antimicrobial Properties ◽  
Dynamic Contact ◽  
Bacterial Inactivation ◽  
E Coli ◽  
Enhanced Production ◽  
Prolonged Contact ◽  
Species Production

Bacterial proliferation and biofilm formation has emerged as a significant concern in the long-term use of industrial apparatus. This study describes the antimicrobial properties of a novel chitosan-polyethyleneimine-graphene oxide (CS-PEI-GO) nanocomposite against E. coli. The nanocomposite is a stable material with minimal dispersibility in storage water after more than 7 days. The antimicrobial activity is contact-time-dependent, with direct contact (92% bacterial inactivation after 3h exposure) having superior results compared with dynamic contact (~50% inactivation after 3h exposure). In addition, the incorporation of GO also translated to enhanced production of ROS—oxidation of GSH was higher in CS-PEI-GO (31.78%) as compared to CS-PEI alone (5.69%). This may be attributed to previously proposed mechanisms of mechanical membrane damage and reactive oxygen species production that may be more pronounced with prolonged contact. This may be due to the positively charged chitosan and the negatively charged cell membrane facilitating the coating of cells that could allow the oxygen-containing functional groups of GO to induce oxidative stress and lead to cell death.

Antimicrobial Activity of Photoactive Cerium Doped Zinc Oxide

2020 ◽  
Vol 307 ◽  
pp. 217-222 ◽  
Author(s):  
Maishara Syazrinni Rooshde ◽  
Wan Rafizah Wan Abdullah ◽  
Amie Zaidah Amran ◽  
Noradhiha Farahin Ibrahim ◽  
Fazilah Ariffin ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Uv Light ◽  
Light Exposure ◽  
Antimicrobial Properties ◽  
Diffusion Method ◽  
Gram Positive ◽  
E Coli ◽  
Antifouling Agent ◽  
Doped Zno ◽  
Zno Powder

Biofouling and biofilms exist as ubiquitous, undesirable accumulation of flora and fauna upon a given substrate when being immersed into an aquatic medium. Therefore, a novel antifouling based materials with the incorporation of nanotechnology has been developed for the prevention of biofouling in its initial stage through photocatalytic treatment. This study investigated the antimicrobial properties of photoactive Cerium (Ce) doped ZnO powder and explores its potential properties for future antifouling application. ZnO nanoparticles was doped with 0.4 mol% Ce was synthesized through the combination of modified citrate gelation technique and solid state sintering. The successful preparation of Ce doped ZnO was confirmed by XRD and SEM. The antimicrobial activity of Ce doped ZnO against E. coli and S. aureus was determined through antibacterial susceptibility test by agar well diffusion method whilst its photocatalytic inactivation efficiency against selected bacteria was analysed through photodegradation testing under UV light irradiation. The findings demonstrated that the synthesized Ce doped ZnO powder exhibited antibacterial effect against Gram-positive bacteria (S. aureus) and excellent photocatalytic efficiency to inactivate both Gram-negative (E. coli) and Gram-positive (S. aureus). 2 g/L of Ce doped ZnO catalyzed the 100% disinfection of both bacteria in 180 min of UV light exposure. Thus, this proved that Ce doped ZnO powder has the potential as efficient antifouling agent.

scholarly journals 3-Amino-5-(indol-3-yl)methylene-4-oxo-2-thioxothiazolidine Derivatives as Antimicrobial Agents: Synthesis, Computational and Biological Evaluation

2020 ◽  
Vol 13 (9) ◽  
pp. 229
Author(s):  
Volodymyr Horishny ◽  
Victor Kartsev ◽  
Vasyl Matiychuk ◽  
Athina Geronikaki ◽  
Petrou Anthi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Antimicrobial Agents ◽  
Biological Evaluation ◽  
Minimal Bactericidal Concentration ◽  
Active Compounds ◽  
Hek 293 ◽  
E Coli ◽  
Thiazolyl Blue Tetrazolium Bromide

Herein we report the design, synthesis, computational, and experimental evaluation of the antimicrobial activity of fourteen new 3-amino-5-(indol-3-yl) methylene-4-oxo-2-thioxothiazolidine derivatives. The structures were designed, and their antimicrobial activity and toxicity were predicted in silico. All synthesized compounds exhibited antibacterial activity against eight Gram-positive and Gram-negative bacteria. Their activity exceeded those of ampicillin and (for the majority of compounds) streptomycin. The most sensitive bacterium was S. aureus (American Type Culture Collection ATCC 6538), while L. monocytogenes (NCTC 7973) was the most resistant. The best antibacterial activity was observed for compound 5d (Z)-N-(5-((1H-indol-3-yl)methylene)-4-oxo-2-thioxothiazolidin-3-yl)-4-hydroxybenzamide (Minimal inhibitory concentration, MIC at 37.9–113.8 μM, and Minimal bactericidal concentration MBC at 57.8–118.3 μM). Three most active compounds 5d, 5g, and 5k being evaluated against three resistant strains, Methicillin resistant Staphilococcus aureus (MRSA), P. aeruginosa, and E. coli, were more potent against MRSA than ampicillin (MIC at 248–372 μM, MBC at 372–1240 μM). At the same time, streptomycin (MIC at 43–172 μM, MBC at 86–344 μM) did not show bactericidal activity at all. The compound 5d was also more active than ampicillin towards resistant P. aeruginosa strain. Antifungal activity of all compounds exceeded those of the reference antifungal agents bifonazole (MIC at 480–640 μM, and MFC at 640–800 μM) and ketoconazole (MIC 285–475 μM and MFC 380–950 μM). The best activity was exhibited by compound 5g. The most sensitive fungal was T. viride (IAM 5061), while A. fumigatus (human isolate) was the most resistant. Low cytotoxicity against HEK-293 human embryonic kidney cell line and reasonable selectivity indices were shown for the most active compounds 5d, 5g, 5k, 7c using thiazolyl blue tetrazolium bromide MTT assay. The docking studies indicated a probable involvement of E. coli Mur B inhibition in the antibacterial action, while CYP51 inhibition is likely responsible for the antifungal activity of the tested compounds.

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