Differential expression of P63, P27, P57, Ki-67, and CD146 in hydropic and molar pregnancies

2021 ◽  
pp. 1-16
Author(s):  
Mahi Balci ◽  
Gulhan Ozdemir ◽  
Admin
Keyword(s):  
Proliferative Activity ◽  
Hydatidiform Mole ◽  
The Body ◽  
Invasion And Metastasis ◽  
Ki 67 ◽  
Immunohistochemical Markers ◽  
Hydropic Abortion ◽  
Proliferation And Differentiation ◽  
Positive Immunostaining ◽  
Activity Location

Abstract Objective: In this study, we investigated expressions of immunohistochemical markers P63, P27, P57, Ki-67, and CD146, in hydropic and molar specimens, to explore their role in pathogenesis of molar gestations. Design and Setting: Retrospective study. Methods: We enrolled 37 patients with a definitive pathologic diagnosis of HA (n = 10), PHM (n = 17), and CHM (n = 10). We scored immunoreactivity using antibodies against P63, P27, P57, Ki-67, and CD146 by evaluating the percentage of distinctly stained cells. Results.Compared to PHM and HA patients, CHM patients had severe cytologic atypia in CD146-positive extravillous trophoblastic column (TC) and florid syncytiotrophoblast (ST) proliferation. P57 immunostaining was negative in all but one of the CHM patients, whereas all HA and PHM patients showed positive immunostaining. PHM and HA patients also showed P63 and Ki-67 overexpression in cytotrophoblasts (CTs) compared to CHM patients. P27 was expressed in differentiated, nondividing syncytiotrophoblasts but did not yield any diagnostic aid. Conclusion: The proliferative activity location varied between molar and nonmolar pregnancies. PHM and HA patients have more progenitor cells, which express the highest levels of P63 compared to CHM patients. The loss of endogenous P63 expression in CHM cases may result in up-regulated genes, associated with invasion and metastasis, predisposing the body to a loss of epithelial and acquisition of mesenchymal characteristics.P63 deficiency, like P57 deficiency, induces exaggerated proliferation and differentiation and could be used as an ancillary diagnostic tool for CHM diagnosis. Keyword: Hydatidiform mole, hydropic abortion, p63, p27, p57, Ki-67, CD146. Continuous...

scholarly journals Evaluation of Ki-67 Expression in Cases of Hydatidiform Mole and Hydropic Abortion

2017 ◽  
Author(s):  
Rabia Altunbaş ◽  
Hacer Uyanıkoglu ◽  
Muhammet Emin Güldür ◽  
Adnan Incebıyık
Keyword(s):  
Hydatidiform Mole ◽  
Ki 67 ◽  
Hydropic Abortion

Profile features immunohistochemical endometrium in patients uterine form of infertility

2016 ◽  
Vol 3 (4) ◽  
pp. 193-197
Author(s):  
Narina A. Arutyunyan ◽  
T. A Dzhibladze ◽  
V. M Zuev ◽  
A. I Ishchenko ◽  
D. V Bryunin ◽  
...  
Keyword(s):  
Proliferative Activity ◽  
Vegf Expression ◽  
Ki 67 ◽  
Immunohistochemical Markers ◽  
Competent Cells ◽  
Inhibition Of Angiogenesis

The article presented data of the complex examination of 79 women aged of 24-43 years with uterine form of infertility, including the determination of immunohistochemical markers (ER, PR, Ki-67, CD34, CD56, CD68, CD138, VEGF) in endometrium. As a result of the study there was revealed the decline in the number of CD56-positive cells, decrease or no expression of CD138, the increase in expression of CD34, decreased VEGF expression. The deterioration of implantation processes in patients with uterine form of infertility, presumably is related to the low content of immune competent cells and inhibition of angiogenesis, as well as a weak proliferative activity of the endometrium, as evidenced by a decrease expression of Ki-67.

scholarly journals Role of the Immunohistochemical Marker (Ki67) in Diagnosis and Classification of Hydatidiform Mole

2020 ◽  
Vol 18 (3) ◽  
Author(s):  
Hasan Abu Deka FF ◽  
Abd Ali Al Saeng ZH ◽  
Khalid Almukhtar Z
Keyword(s):  
Stromal Cells ◽  
Hydatidiform Mole ◽  
Labeling Index ◽  
Statistical Relationship ◽  
Ki 67 ◽  
Cross Sectional ◽  
Complete Mole ◽  
Partial Mole ◽  
Hydropic Abortion

Introduction: Since the hallmark of gestational trophoblastic disease is trophoblastic proliferation, Ki67 is regarded as the best marker in studying hydatidiform mole.This study was conducted to evaluate the role of this proliferative marker in distinguishing among hydropic abortion, partial and complete hydatidiform mole. Materials and methods: This is a cross sectional study involving the application of Ki67 on a total of 90 histological samples of curetting materials from molar (partial and complete mole) and non molar hydropic abortion belong to Iraqi females, so three study groups were created. Immunohistochemical expression in villous cytotrophoblasts, syncytiotrophoblasts and stromal cells were recorded separately by three independent observers and the results were correlated statically. Results: The mean number of stained nuclei of villous cytotrophoblasts and stromal cells was the highest in complete mole and the lowest in non molar hydropic abortion. There is a significant statistical relationship regarding Ki67 labeling index in villous cytotrophoblasts between partial moles and hydropic abortion, complete mole and partial moles, hydropic abortion and complete mole. Regarding Ki67 labelling index in villous stromal cells, a significant statistical relationship achieved when the correlation done between partial mole and hydropic abortions, hydropic abortion and complete mole, while a non significant statistical relationship was achieved if the correlation done between partial and complete mole. All villous syncytiotrophoblasts showed negative results. Conclusion: Ki-67 labeling index in villous cytotrophblastic cells are useful in separating between partial moles and hydropic abortion, partial mole and complete mole, hydropic abortion and complete mole. While Ki-67 labeling index in villous stromal cells is only useful in separating between partial moles and hydropic abortion, hydropic abortion and complete mole.

scholarly journals STRUCTURAL-FUNCTIONAL REORGANIZATION OF SKIN AND ITS DERIVATIVES UNDER EXPERIMENTAL SUBACUTE OF INTOXICATION BY HEAVY METALS

2019 ◽  
Vol 96 (6) ◽  
pp. 593-596
Author(s):  
Tatyana V. Nikolaeva ◽  
V. S. Polyakova ◽  
N. P. Setko ◽  
L. G. Voronina
Keyword(s):  
Heavy Metals ◽  
Proliferative Activity ◽  
Lead Acetate ◽  
Dermal Papilla ◽  
Hair Follicles ◽  
The Body ◽  
Acetate Solution ◽  
Sodium Dichromate ◽  
Ki 67 ◽  
Nickel Chromium

There was executed an experimental study of the effect of salts of heavy metals (nickel, chromium, lead and zinc) entering the body by peroral route, on the morphology of the skin and its derivatives (hair follicles and sebaceous glands). The experiment was performed on C57BL / 6 mice with the use of the induction of hair follicle cycle by depilation. Under the subacute intoxication with salts of nickel, chromium and lead, there were revealed such signs of a dystrophic anagen as ectopia of granules of melanin in the dermal papilla and perifollicular tissue, enlarged channels of the hair. The duration of the anagen stage if compared with the control did not change. Under the intoxication with salts of nickel and lead there was revealed infiltration by mononuclear dermis and hypodermis. Lead acetate gave rise in the capillary congestion of the dermis, followed by diapedesis of erythrocytes and infiltration of the dermis by siderophages. In the course of the immunohistochemical study of the proliferative activity of keratinocytes of the skin integument derivatives with the use of antibodies to Ki-67, there was revealed a significant increase of proliferative activity of keratinocytes in comparison with the control under the use of a solution of zinc sulphate and sodium dichromate and its decrease with the use of lead acetate solution.

The diagnostic value of Ki-67, P53 and P63 in distinguishing partial Hydatidiform mole from hydropic abortion

2012 ◽  
Vol 124 (5-6) ◽  
pp. 184-187 ◽  
Author(s):  
Yunxin Chen ◽  
Danhua Shen ◽  
Yiqun Gu ◽  
Pingping Zhong ◽  
Junlin Xie ◽  
...  
Keyword(s):  
Hydatidiform Mole ◽  
Diagnostic Value ◽  
Ki 67 ◽  
Partial Hydatidiform Mole ◽  
Hydropic Abortion

scholarly journals The Role of Immunohistochemical Markers for the Diagnosis and Prognosis of Adrenocortical Neoplasms

2021 ◽  
Vol 11 (3) ◽  
pp. 208
Author(s):  
Anna Angelousi ◽  
Georgios Kyriakopoulos ◽  
Fani Athanasouli ◽  
Anastasia Dimitriadi ◽  
Eva Kassi ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Cox Regression ◽  
P53 Protein ◽  
Immunohistochemical Analysis ◽  
Ki 67 ◽  
Diagnosis And Prognosis ◽  
Immunohistochemical Markers ◽  
Small Series ◽  
Morphological Criteria ◽  
Survival And Development

Adrenal cortical carcinoma (ACC) is a rare cancer with poor prognosis that needs to be distinguished from adrenocortical adenomas (ACAs). Although, the recently developed transcriptome analysis seems to be a reliable tool for the differential diagnosis of adrenocortical neoplasms, it is not widely available in clinical practice. We aim to evaluate histological and immunohistochemical markers for the distinction of ACCs from ACAs along with assessing their prognostic role. Clinical data were retrospectively analyzed from 37 patients; 24 archived, formalin-fixed, and paraffin-embedded ACC samples underwent histochemical analysis of reticulin and immunohistochemical analysis of p27, p53, Ki-67 markers and were compared with 13 ACA samples. Weiss and Helsinki scores were also considered. Kaplan−Meier and univariate Cox regression methods were implemented to identify prognostic effects. Altered reticulin pattern, Ki-67% labelling index and overexpression of p53 protein were found to be useful histopathological markers for distinguishing ACAs from ACCs. Among the studied markers, only pathological p53 nuclear protein expression was found to reach statistically significant association with poor survival and development of metastases, although in a small series of patients. In conclusion, altered reticulin pattern and p53/Ki-67 expression are useful markers for distinguishing ACCs from ACAs. Immunohistopathology alone cannot discriminate ACCs with different prognosis and it should be combined with morphological criteria and transcriptome analysis.

Immunohistochemical study of p53 expression in endometrial carcinomas: correlation with markers of proliferating cells and clinicopathologic features

1993 ◽  
Vol 3 (6) ◽  
pp. 363-368 ◽  
Author(s):  
T. Hachisuga ◽  
K. Fukuda ◽  
M. Uchiyama ◽  
N. Matsuo ◽  
T. Iwasaka ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Proliferative Activity ◽  
P53 Protein ◽  
Myometrial Invasion ◽  
Proliferating Cells ◽  
Ki 67 ◽  
Normal Endometrium ◽  
P53 Expression ◽  
Dna Polymerase Α ◽  
Endometrial Carcinomas

Using anti-p53 (PAb1801 and PAb240), anti-DNA polymerase α and Ki-67 monoclonal antibodies, the expression of p53 was studied in 11 normal endometria, 14 endometrial hyperplasias and 27 endometrial carcinomas and its relationship to the proliferative activity of the tumors was examined. Normal endometria and simple hyperplasias were completely negative for p53. The PAb1801 indices of complex hyperplasias and complex atypical hyperplasias were 2.5±1.8% and 5.0±3.2%, respectively. The PAb1801 indices of grade 1, grade 2 and grade 3 endometrial carcinomas were 10.2±14.2%, 44.4±29/0% and 45.0±32.5%, respectively. These results indicate a progressively enhanced p53 expression in the sequence from normal endometrium, through hyperplasia to carcinoma. A significant correlation between p53 expression and labeling indices of Ki-67 and DNA polymerase α was observed in endometrial carcinomas. The endo-metrial carcinomas with p53 overexpression developed mainly in post-menopausal patients and were frequently high-grade tumors with deep myometrial invasion. These findings may indicate that overexpression of p53 protein contributes to the proliferative activity of the tumor cells.

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