scholarly journals Frequency of Dengue Virus Serotype 1 in Lahore by In-house assay

2020 ◽  
pp. 1-7
Author(s):  
Hajra Farooq ◽  
Waheed Uz Zaman Tariq ◽  
Sadia Bano ◽  
Ali Raza ◽  
Omar Rasheed Chughtai ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Dengue Virus ◽  
Real Time Pcr ◽  
Fast Track ◽  
Virus Rna ◽  
Virus Serotype ◽  
Serotype 1 ◽  
Dengue Virus Serotype 1 ◽  
Commercial Kit ◽  
Dengue Epidemic

Abstract Dengue is an important systemic viral infection that is caused by the dengue virus. Ribonucleic acid (RNA) from dengue NS1 positive samples, collected randomly during dengue epidemic from October 2016 to October 2017 at Chugtai Lab, was extracted for nucleic acid. Both the detection and serotyping of dengue samples were performed using real-time PCR on Rotor Gene Q. From the 70 NS1 positive samples, 57 (81.4%) samples were confirmed to be positive for dengue virus RNA, while the remaining 13 (18.6%) were negative. Serotype 1 (DEN-1) were verified among all samples by in-house assay and using commercial kit FTD (Fast Track Diagnostics) dengue differentiation; it was concluded that our in-house assay is in 100% concordance with commercial kit. Serotype 2 (DEN-2) and serotype 3 (DEN-3) have been documented in Pakistan since 1994. Continuous...  

Induction of multiple cytotoxic T lymphocyte responses in mice by a multiepitope DNA vaccine against dengue virus serotype 1

2016 ◽  
Vol 60 (12) ◽  
pp. 835-845 ◽  
Author(s):  
Xin Yu Chen ◽  
De Zhou Li ◽  
Xiao Zhi Zhong ◽  
Bokun Chen ◽  
Zhi Liang Duan ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Dna Vaccine ◽  
T Lymphocyte ◽  
Cytotoxic T Lymphocyte ◽  
Virus Serotype ◽  
Serotype 1 ◽  
Lymphocyte Responses ◽  
Dengue Virus Serotype 1

scholarly journals Safety and Immunogenicity of a Dengue Virus Serotype-1 Purified-Inactivated Vaccine: Results of a Phase 1 Clinical Trial

2015 ◽  
Vol 93 (3) ◽  
pp. 454-460 ◽  
Author(s):  
Luis Javier Martinez ◽  
Leyi Lin ◽  
Jason M. Blaylock ◽  
Arthur G. Lyons ◽  
Kristen M. Bauer ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Dengue Virus ◽  
Phase 1 ◽  
Inactivated Vaccine ◽  
Phase 1 Clinical Trial ◽  
Virus Serotype ◽  
Serotype 1 ◽  
Dengue Virus Serotype 1

scholarly journals Rapid Subtyping of Dengue Virus Serotypes 1 and 4 by Restriction Site-Specific PCR

2000 ◽  
Vol 38 (3) ◽  
pp. 1286-1289 ◽  
Author(s):  
Marize P. Miagostovich ◽  
Flavia B. dos Santos ◽  
C. Milena Gutiérrez ◽  
Lee W. Riley ◽  
Eva Harris
Keyword(s):  
Sequence Analysis ◽  
Dengue Virus ◽  
Restriction Site ◽  
Epidemiological Studies ◽  
Site Specific ◽  
Specific Pcr ◽  
Virus Serotype ◽  
Serotype 1 ◽  
Dengue Virus Serotypes ◽  
Dengue Virus Serotype 1

We previously reported a simple subtyping method, restriction site-specific PCR (RSS-PCR), for dengue virus serotypes 2 and 3; here we describe its application for subtyping dengue virus serotypes 1 and 4. Three major RSS-PCR types were observed for dengue virus serotype 1 and two types were observed for dengue virus serotype 4, in agreement with previous strain classifications based on sequence analysis. Because of its simplicity, this method is amenable to rapid subtyping and application to epidemiological studies of dengue in countries where dengue is endemic.

scholarly journals Complete Genome Sequence Analysis of an Imported Dengue Virus Serotype 1 Strain from Myanmar

2018 ◽  
Vol 6 (27) ◽  
Author(s):  
Zhaoping Zeng ◽  
Jiandong Shi ◽  
Xiaofang Guo ◽  
Ling Mo ◽  
Ningzhu Hu ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Genome Sequence Analysis ◽  
Virus Serotype ◽  
Serotype 1 ◽  
Complete Genome Sequence Analysis ◽  
Dengue Virus Serotype 1

It has been determined that recent dengue virus epidemics in Yunnan, China, originated from Southeast Asian strains. Here, we report the first complete genome sequence and molecular characterization of the imported dengue virus serotype 1 strain YNPE1.

Resistance analysis of an antibody that selectively inhibits dengue virus serotype-1

2012 ◽  
Vol 95 (3) ◽  
pp. 216-223 ◽  
Author(s):  
Gang Zou ◽  
Petra Kukkaro ◽  
Shee-Mei Lok ◽  
Jowin K.W. Ng ◽  
Grace K. Tan ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Resistance Analysis ◽  
Virus Serotype ◽  
Serotype 1 ◽  
Dengue Virus Serotype 1

Identification of cytotoxic T lymphocyte epitopes in dengue virus serotype 1

2015 ◽  
Vol 87 (7) ◽  
pp. 1077-1089 ◽  
Author(s):  
Zhiliang Duan ◽  
Jianglong Guo ◽  
Xi Huang ◽  
Huifang Liu ◽  
Xinyu Chen ◽  
...  
Keyword(s):  
Dengue Virus ◽  
T Lymphocyte ◽  
Cytotoxic T Lymphocyte ◽  
Virus Serotype ◽  
Serotype 1 ◽  
Dengue Virus Serotype 1
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