scholarly journals Identification and mRNA Expression of Heat Shock Proteins in the Mud Crab (Scylla paramamosain) in Response to Acute Nitrite Exposure

2018 ◽  
Author(s):  
Z-X. Guo ◽  
X.-L. Chena ◽  
Y-Q. Denga ◽  
Y-L. Sua ◽  
J. Fenga ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Mrna Expression ◽  
Scylla Paramamosain ◽  
Mud Crab ◽  
Shock Proteins

Study of Cell-Lipid Film Interaction by Measuring Heat Shock Proteins mRNA Expression

2000 ◽  
Vol 15 (6) ◽  
pp. 478-488 ◽  
Author(s):  
AKIO KISHIDA ◽  
TAKAHISA MATSUYAMA ◽  
MIHOKO NAKASHIMA ◽  
TAKESHI SERIZAWA ◽  
MITSURU AKASHI ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Mrna Expression ◽  
Lipid Film ◽  
Cell Lipid ◽  
Shock Proteins

scholarly journals Identification of Equine Heat Shock Proteins Gene and Their mRNA Expression Analysis after Exercise

2014 ◽  
Vol 24 (2) ◽  
pp. 105-111 ◽  
Author(s):  
Hyun-Woo Cho ◽  
Jeong-Woong Park ◽  
Jae-Young Choi ◽  
S. Sivakumar ◽  
Nam-Young Kim ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Mrna Expression ◽  
Expression Analysis ◽  
Mrna Expression Analysis ◽  
Shock Proteins

Effects of chronic portal hypertension on small heat-shock proteins in mesenteric arteries

2005 ◽  
Vol 288 (4) ◽  
pp. G616-G620 ◽  
Author(s):  
Xuesong Chen ◽  
Hai-Ying Zhang ◽  
Kristin Pavlish ◽  
Joseph N. Benoit
Keyword(s):  
Portal Hypertension ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Mrna Expression ◽  
Protein Level ◽  
Small Heat Shock Proteins ◽  
Mesenteric Arteries ◽  
Protein Levels ◽  
Small Hsps ◽  
Shock Proteins

Previous studies have shown that impaired vasoconstrictor function in chronic portal hypertension is mediated via cAMP-dependent events. Recent data have implicated two small heat-shock proteins (HSP), namely HSP20 and HSP27, in the regulation of vascular tone. Phosphorylation of HSP20 is associated with vasorelaxation, whereas phosphorylation of HSP27 is associated with vasoconstriction. We hypothesized that alterations in the expression and/or phosphorylation of small HSPs may play a role in impaired vasoconstriction in portal hypertension. A rat model of prehepatic chronic portal hypertension was used. Studies were conducted in small mesenteric arteries isolated from normal and portal hypertensive rats. Protein levels of HSP20 and HSP27 were detected by Western blot analysis. Protein phosphorylation was analyzed by isoelectric focusing. HSP20 mRNA expression was determined by RT-PCR. To examine the role of cAMP in the regulation of small HSP phosphorylation and expression, we treated both normal and portal hypertensive vessels with a PKA inhibitor Rp-cAMPS. We found both an increased HSP20 phosphorylation and a decreased HPS20 protein level in portal hypertension, both of which were restored to normal by PKA inhibition. However, PKA did not change HSP20 mRNA expression. We conclude that decreased HSP20 protein level is mediated by cAMP-dependent pathway and that impaired vasoconstrictor function in portal hypertension may be partially explained by decreased expression of HSP20. We also suggest that the phosphorylation of HSP20 by PKA may alter HSP20 turnover.

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