scholarly journals Molecular Identification of Green Algae, Spirogyra Porticalis, along Parts of River Kaduna and its Potential for Singlecell Protein (SCP) Production

2019 ◽  
Vol 1 ◽  
pp. 38-43
Author(s):  
A A Haroun ◽  
I K Matazu ◽  
Y Abdulhamid ◽  
J Sani

The freshwater alga spirogyra porticalis, a filamentous chlorophyte, was collected and identified on the basis of morphology and molecular characteristics. The nutritional composition of the Alga (protein, carbohydrate, moisture, fat, ash) were determined using the AOAC, Official Methods of Analysis. The components of Single Cell Protein (SCP) were determined using method of proximate analysis. The total content of protein (% dry weight) ranged from 12.46-16.89%, carbohydrate from 34.72-39.25%, moisture from 12.97-16.75%, fat from 20.17-22.03%, ash from 10.78-15.98%. The result revealed that this Macroalga has an appreciable amount of protein, carbohydrate, moisture, fat, and ash. Amino acids composition determines the protein quality of Algae. Hence, the amino acids present in the algal sample were extracted and estimated using an automated amino acids Analyzer. The results have shown the presence of the following amino acids; Leu, Lys, Ile, Phe, Val, His, Arg, Met, Trp, Thr, Pro, Tyr, Gly, Glu, Asp, Cys, Ser, Ala. The essential amino acids (Leu, Lys, Ile, Phe, Val, His, Arg, Met, Trp, Thr) profile of the alga as compared with conventional protein sources and the WHO/FAO reference pattern of essential amino acids (g per 100 protein), reveals that the levels of the essential amino acids in the alga were either similar to or greater than the levels of the same amino acids in the reference foods and WHO/FAO reference pattern. Thus, results of the present study shows that S.porticalis is a potential source of single cell protein, and may be of use to the food industry as a source of ingredients with high nutritional value. S. porticalis can provide a dietary alternative due to its nutritional value and its commercial value can be enhanced by improving the quality and expanding the range of freshwater macroalgae based products.

2018 ◽  
Vol 17 (23) ◽  
pp. 716-723 ◽  
Author(s):  
Kounbesioune SOMDA Marius ◽  
NIKIEMA Mahamadi ◽  
KEITA Ibrahim ◽  
MOGMENGA Iliassou ◽  
H. S. KOUHOUNDE Sonagnon ◽  
...  

Aquaculture ◽  
2021 ◽  
Vol 531 ◽  
pp. 735885 ◽  
Author(s):  
Muhammad Sharif ◽  
Muhammad Hammad Zafar ◽  
Amjad Islam Aqib ◽  
Muhammad Saeed ◽  
Mayada R. Farag ◽  
...  

2012 ◽  
Vol 108 (S2) ◽  
pp. S333-S336 ◽  
Author(s):  
Gertjan Schaafsma

PDCAAS is a widely used assay for evaluating protein quality. It is a chemical score, which is derived from the ratio between the first limiting amino acid in a test protein and the corresponding amino acid in a reference amino acid pattern and corrected for true faecal N digestibility. Chemical scores exceeding 100 % are truncated to 100 %. The advantages of the PDCAAS are its simplicity and direct relationship to human protein requirements. The limitations are as follows: the reference pattern is based on the minimum amino acid requirements for tissue growth and maintenance and does not necessarily reflect the optimum intake. Truncated PDCAAS of high-quality proteins do not give any information about the power of these proteins to compensate, as a supplement, for low levels of dietary essential amino acids in low-quality proteins. It is likely that faecal N digestibility does not take into account the loss from the colon of indispensable amino acids that were not absorbed in the ileum. Anti-nutritional factors, such as lectins and trypsin inhibitors, in several plant protein sources can cause heightened endogenous losses of amino acids, an issue which is particularly relevant in animal feedstuffs. The assumption that amino acid supplementation can completely restore biological efficiency of the protein source is incorrect since the kinetics of digestion and absorption between supplemented free amino acids and amino acids present in dietary proteins, are different.


1993 ◽  
Vol 41 (1) ◽  
pp. 57-60
Author(s):  
Norma. Samman ◽  
Oscar E. Molina ◽  
Nora I. Perotti de Galvez ◽  
Danley A. S. Callieri ◽  
Ricardo N. Farias

Author(s):  
T.V. Mazhaeva ◽  
S.E. Dubenko

To decrease the toxic effects of xenobiotics on healthy workers in healthful and dietary meals, it’s important to consider not only the proper diet intake of macro- and micronutrients, but protein quality in terms of essential amino acids. A study was conducted to evaluate nutritional value and amino acids content in the industrial workers diets of two metallurgical enterprises by calculation and laboratory methods. We found that set of healthful and dietary meals at the calculation stage do not comply with regulatory requirements for fats and carbohydrates by 34 and 16 percent, respectively. According to laboratory tests, from 57 to 68 percent of sets of healthful and dietary meals do not comply with regulatory requirements for proteins, fats and carbohydrates. The discrepancy in the data on the amount of determined nutrients between the calculated and laboratory methods may be due to calculation errors, shortage and quality of raw materials, as well as a limitation of methods for determining fat and carbohydrates. With a set of healthful and dietary meals workers can satisfy the almost daily requirement for the corresponding energy expenditures for some amino acids, however, the required amount of these amino acids in workers in contact with heavy metals has not been established. It has also been found that in diets of healthful and dietary meals, the ratio of tryptophan / lysine / methionine + cystine is violated towards an excess of tryptophan, whose deamination products are highly toxic. It is necessary to continue further investigation of ready-made diets of healthful and dietary meals for nutritional value and amino acid content in order to identify factors affecting the discrepancy within research results between calculation and laboratory methods, as well as establish adequate norms for amino acids intake in workers exposed to toxic substances.


Author(s):  
Nura Abdullahi ◽  
Munir Abba Dandago ◽  
Alkasim Kabiru Yunusa

The roles of protein in bodybuilding and the regulation of biological processes are important in sustaining life. A large amount of protein is required by both humans and animals and this cannot be supplied by only conventional sources. This is because of the rapid increase in world population. The present sources of protein will not meet global protein demand in years to come. Scientists explore the production of single-cell protein (SCP), as an alternative source of protein, through the utilization of wastes and low-value materials. SCP can supply high-quality protein containing both essential and non-essential amino acids that can be utilized by humans and animals. Protein from microbial biomass is cheaper than animal proteins because the substrates used in the production are generally cheaper and more readily available. Moreover, the production process does not require arable land and the entire process can be completed within a short time. This article reviewed the process of SCP production. Different raw materials used in the production and variations in growth media preparation methods were discussed. Various sources of fermentation microorganisms and their potential substrate were reviewed. Growth media enrichment using different carbon, nitrogen, and mineral sources was also discussed.


1991 ◽  
Vol 74 (1) ◽  
pp. 104-106
Author(s):  
Abdul Aziz ◽  
Faisal Abu-Dagga

Abstract A commercially available single-cell protein (Pruteen) was analyzed for amino acids, fatty acids, and major, minor, and trace-element composition after storage at room temperature and at 4°C over a period of 5 years. The purpose was to assess its suitability as an organic-nutrient standard reference material for food analysis. The material showed longterm stability and is, therefore, recommended for interlaboratory certification studies.


1977 ◽  
Vol 42 (5) ◽  
pp. 1247-1250 ◽  
Author(s):  
H. H. YANG ◽  
S. P. YANG ◽  
D. W. THAYER

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