scholarly journals Production of Bioethanol from Cassava using Zymomonas mobilis: Effect of Temperature and Substrate concentration

2019 ◽  
Vol 1 ◽  
pp. 153-160
Author(s):  
I J Ona ◽  
H O Agogo ◽  
M S Iorungwa
Keyword(s):  
Zymomonas Mobilis ◽  
Simultaneous Saccharification And Fermentation ◽  
Ethanol Yield ◽  
Effect Of Temperature ◽  
Cassava Flour ◽  
Gram Negative ◽  
Theoretical Yield ◽  
Ethanol Yields ◽  
Theoretical Ethanol Yield ◽  
Simultaneous Saccharification

The production of ethanol from cassava flour using Zymomonas mobilis a gram negative bacterium was conducted at 30oC, 33oC, 35oC and 37oC. The fermentation reaction was also carried out at different substrate concentrations; 5% W/V, 7% W/V and 10% W/V. The microorganism Zymomonas mobilis was detected in palm wine, isolated and identified. It was found to be gram negative, oxidase negative, catalase positive, anaerobic and plump rods with an unusual width. Results obtained from the simultaneous saccharification and fermentation reactions carried out with Zymomonas mobilis showed that maximum theoretical ethanol yield of 63% was obtained for 7% W/V cassava flour at 35oC. This was followed by a theoretical yield of 56.23 and 54.12 for 5% W/V and 10% W/V cassava flour, respectively. Fermentations at 30oC and 33 oC gave similar results with 7% W/V cassava producing higher ethanol yield when compared to 5% W/V and 10% W/V. Fermentation reactions at 37oC gave the lowest ethanol yields. The optimum pH for the simultaneous saccharification and fermentation of cassava was found to be pH of 6.

scholarly journals Production of bioethanol from sago hampas via Simultaneous Saccharification and Fermentation (SSF)

2018 ◽  
Vol 10 (4) ◽  
pp. 240-245 ◽  
Author(s):  
HUANG CHAI HUNG ◽  
DAYANG SALWANI AWANG ADENI ◽  
QUEENTETY JOHNNY ◽  
MICKY VINCENT
Keyword(s):  
Simultaneous Saccharification And Fermentation ◽  
Ethanol Yield ◽  
Cellulolytic Enzymes ◽  
Total Biomass ◽  
Bioethanol Production ◽  
Amylolytic Enzymes ◽  
Theoretical Ethanol Yield ◽  
Waste Accumulation ◽  
Biomass Reduction ◽  
Simultaneous Saccharification

Huang CH, Adeni DSA, Johnny Q, Vincent M. 2018. Production of bioethanol from sago hampas via Simultaneous Saccharification and Fermentation (SSF). Nusantara Bioscience 10: 240-245. Sago hampas is an inexpensive, renewable and abundant agro-industrial residue that can be exploited to produce bioethanol. In this study, ethanol production was performed via simultaneous saccharification and fermentation (SSF) on fresh sago hampas at 2.5%, 5.0% and 7.5% (w/v) feedstock loadings with the aid of amylolytic enzymes, cellulolytic enzymes and Saccharomyces cerevisiae, under anaerobic condition for five days with a constant agitation of 150 rpm and ambient temperature. Results obtained indicated that SSF with 5.0% (w/v) sago hampas loading produced the highest ethanol yield at 17.79 g/L (79.65% Theoretical Ethanol Yield, TEY), while SSF using 2.5% and 7.5% (w/v) sago hampas produced ethanol at only 8.38 g/L (75.00% TEY) and 23.28 g/L (69.48% TEY), respectively. Total biomass reduction was recorded between 66.3% to 71.3% by the end of the SSF period. This study demonstrated that fresh sago hampas is a promising feedstock for bioethanol production as yields are generally high for all the substrate loadings tested. Moreover, bioethanol production using fresh sago hampas may assist in reducing pollution caused by sago waste accumulation.

scholarly journals Bioethanol Production From Pineapple Wastes

2014 ◽  
Vol 3 (4) ◽  
pp. 60 ◽  
Author(s):  
Alessia Tropea ◽  
David Wilson ◽  
Loredana G. La Torre ◽  
Rosario B. Lo Curto ◽  
Peter Saugman ◽  
...  
Keyword(s):  
Plant Cell ◽  
Cell Walls ◽  
Simultaneous Saccharification And Fermentation ◽  
Ethanol Yield ◽  
Enzymatic Saccharification ◽  
Plant Cell Walls ◽  
Theoretical Yield ◽  
Cellulosic Sugars ◽  
Separate Hydrolysis And Fermentation ◽  
Simultaneous Saccharification

<p>There is great interest in producing bioethanol from biomass and there is much emphasis on exploiting lignocellulose sources, from crop wastes through to energy-rich crops. Some waste streams, however, contain both cellulosic and non-cellulosic sugars. These include wastes from pineapple processing.</p> <p>Pineapple wastes are produced in large amounts throughout the world by canning industries. These wastes are rich in intracellular sugars and plant cell walls which are composed mainly of cellulose, pectic substances and hemicelluloses. The purpose of this study was to investigate the potential to transform such residues into ethanol after enzymatic saccharification of plant cell walls, and fermentation of the resulting simple sugars using the <em>Saccharomyces cerevisiae</em> NCYC 2826 strain. Three different fermentation modes, direct fermentation, separate hydrolysis and fermentation, and simultaneous saccharification and fermentation of the biomass were tested and compared. The results show that the main sugars obtained from pineapple waste were: glucose, uronic acid, xylose, galactose, arabinose and mannose. The highest ethanol yield was achieved after 30 hours of simultaneous saccharification and fermentation, and reached up to 3.9% (v/v), corresponding to the 96% of the theoretical yield.</p>

scholarly journals Effect of Pre-hydrolysis on Simultaneous Saccharification and Fermentation of Native Rye Starch

2020 ◽  
Vol 13 (6) ◽  
pp. 923-936 ◽  
Author(s):  
Ewelina Strąk-Graczyk ◽  
Maria Balcerek
Keyword(s):  
Dry Matter ◽  
Agricultural Sector ◽  
Simultaneous Saccharification And Fermentation ◽  
Ethanol Yield ◽  
Matter Content ◽  
Raw Material ◽  
Process Efficiency ◽  
Dry Matter Content ◽  
Sem Images ◽  
Simultaneous Saccharification

Abstract The rising population and increasing demand for food place added pressure on the agricultural sector to maintain high process efficiency while implementing environmentally friendly methods. In this study, we investigate the effect of pre-hydrolysis of native rye starch and its influence on the yield of ethanol obtained by simultaneous saccharification and fermentation (SSF) from high gravity rye mashes with 25% and 28% w w−1 dry matter content. Fermentation was carried out in a 3-day system at a temperature of 35 ± 1 °C using the dry distillery yeast Ethanol Red (Saccharomyces cerevisiae). The characteristics of the tested raw material and changes in the native rye starch during enzymatic hydrolysis were analyzed using a scanning electron microscope (SEM). The SEM images revealed characteristic changes on the surface of the starch, which was found to have a layered structure, as well as interesting behavior by the yeast during SSF when the glucose concentration in the environment was lowered. Both in the mashes with 25% and 28% w w−1 dry matter, starch pre-hydrolysis did not significantly increase either the initial amounts of sugars available to the yeast or the fermentation efficiency and ethanol yield in comparison to the mashes without this pre-treatment.

Fuel Ethanol Production from Wet Oxidised Corn Stover by S. Cerevisiae

2011 ◽  
Vol 343-344 ◽  
pp. 963-967 ◽  
Author(s):  
Zhang Qiang ◽  
Anne Belinda Thomsen
Keyword(s):  
Ethanol Production ◽  
Corn Stover ◽  
Simultaneous Saccharification And Fermentation ◽  
Ethanol Concentration ◽  
Ethanol Yield ◽  
Liquid Fraction ◽  
Raw Material ◽  
Wet Oxidation ◽  
Inhibition Effect ◽  
Simultaneous Saccharification

In order to find out appropriate process for ethanol production from corn stover, wet oxidation（195°C，15 minutes）and simultaneous saccharification and fermentation (SSF) was carried out to produce ethanol. The results showed that the cellulose recovery of 92.9% and the hemicellulose recovery of 74.6% were obtained after pretreatment. 86.5% of cellulose was remained in the solid cake . After 24h hydrolysis at 50°C using cellulase（Cellubrix L）,the achieved conversion of cellulose to glucose was 64.8%. Ethanol production was evaluated from dried solid cake and the hydrolysate was employed as liquid fraction . After 142 h of SSF with substrate concentration of 8% (W/V), ethanol yield of 73.1 % of the theoretical based on glucose in the raw material was obtained by S. cerevisiae(ordinary baker’ yeast) . The corresponding ethanol concentration and volumetric productivity were 17.2g/L and 0.121g/L.h respectively. The estimated total ethanol production was 257.7 kg/ton raw material by assuming consumption of both C-6 and C-5. No obvious inhibition effect occurred during SSF. These instructions give you the basic guidelines for preparing papers for WCICA/IEEE conference proceedings.

scholarly journals Production of ethanol from Jerusalem artichoke by mycelial pellets

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Chao Zhang ◽  
Daoji Wu ◽  
Hongqi Yang ◽  
Huixue Ren
Keyword(s):  
Lower Cost ◽  
Simultaneous Saccharification And Fermentation ◽  
Volume Fraction ◽  
Ethanol Yield ◽  
New Technology ◽  
Jerusalem Artichoke ◽  
Mycelial Pellets ◽  
Repeated Use ◽  
Easy Operation ◽  
Simultaneous Saccharification

AbstractMycelial pellets formed by Aspergillus niger A-15 were used to immobilize the ethanol producing yeast Saccharomyces cerevisiae C-15. The operation parameters, such as agitation speed, temperature and mixed proportion of strains were studied. The optimal adsorption 66.9% was obtained when speed was 80r/min, temperature was 40 °C and mixed proportion(mycelial pellets: yeasts) was 1:10. With Jerusalem artichoke flour as substrate, 12.8% (V/V) of ethanol was obtained after 48 h by simultaneous saccharification and fermentation using mycelial pellets. And mycelial pellets could tolerate 19% (volume fraction) ethanol. The above results proved that this new technology was feasible, and it had the advantages of higher ethanol yield, long service life, repeated use, easy operation and lower cost in producing ethanol.

Comparison of Bacterial and Yeast Ethanol Fermentation Yield from Rice Straw

2011 ◽  
Vol 347-353 ◽  
pp. 2541-2544
Author(s):  
Benjarat Laobussararak ◽  
Warawut Chulalaksananukul ◽  
Orathai Chavalparit
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Enzymatic Hydrolysis ◽  
Rice Straw ◽  
Zymomonas Mobilis ◽  
Ethanol Yield ◽  
Sugar Content ◽  
Alkali Pretreatment ◽  
Yeast Saccharomyces Cerevisiae ◽  
Pretreatment Condition ◽  
Theoretical Yield

This study was to investigate the fermentation of rice straw using various microorganisms, i.e., the bacterium Zymomonas mobilis, a distillery yeast Saccharomyces cerevisiae and a co-culture of Zymomonas mobilis and Saccharomyces cerevisiae. Rice straw was pretreated with alkaline and followed by enzymatic hydrolysis using cellulase before fermentation by the bacterium and a distillery yeast. Results show that alkali pretreatment is appropriate for rice straw since this pretreatment condition can produce the maximum cellulose of 88.96% and reducing sugar content of 9.18 g/l. Furthermore, the ethanol yield after enzymatic hydrolysis (expressed as % theoretical yield) was 15.94-19.73% for the bacterium, 20.48-35.70% for yeast and 21.56-29.89% for co-culture. Therefore, the distillery yeast was a suitable microorganism for ethanol production from rice straw.

scholarly journals Production of Bioethanol from Selected Lignocellulosic Agrowastes

2019 ◽  
pp. 1-17
Author(s):  
Olotu Emmanuel Juwon ◽  
Olukunle Folake Oluwatoyin
Keyword(s):  
Simultaneous Saccharification And Fermentation ◽  
Ethanol Yield ◽  
Titratable Acidity ◽  
Reducing Sugar ◽  
Corn Cobs ◽  
Orange Peels ◽  
Total Titratable Acidity ◽  
Cassava Peels ◽  
Bioethanol Yield ◽  
Simultaneous Saccharification

This study evaluated the ability of cassava peels, banana peels, orange peels and corn cobs hydrolysates to produce bioethanol. Fibre fractions analysis was carried out using standard methods. The samples were pre-treated with acid and base, followed by simultaneous saccharification and fermentation (SSF) for bioethanol production. During fermentation, pH, total titratable acidity, reducing sugar, microbial load and bioethanol yield were determined. The reducing sugar yield for Aspergillus niger and Bacillus cereus were 30.28 g and 13.35 g for corn cobs. The pH was observed to decrease during fermentation period with orange peels having the lowest pH of 2.6 after 240 hours of fermentation using A. Niger and S. cerevisiae, when B. cereus and S. Cerevisiae were used the pH was observed to be 4.10.  Total titratable acidity showed increase in all the substrates, with corn cobs having the highest when B. cereus and S. cerevisiae were used (1.62), followed by cassava peels when A. niger and S. cerevisiae were used (1.52). Highest ethanol yield following simultaneous saccharification and fermentation with A. niger and S. cerevisiae was obtained in corn cobs with 17.43 g/100 g, while orange peels gave the lowest with 8.02 g/100 g, the ethanol yield from each substrates as well as the combined substrates were significantly different at p≤ 0.05. The combined substrates (1:1:1:1) gave the highest ethanol yield of 12.44 g/100 g using A. niger and S. cerevisiae.  This study therefore revealed that A. niger had the highest bioethanol yield using corn cobs as the carbon source, therefore it could be used for mass bioethanol production.

scholarly journals Assessing Ethanol Yield from Fermented Juice of Local and Exotic Sweet Sorghum Varieties Grown in Sudan

2021 ◽  
Vol 12 ◽  
pp. 11-17
Author(s):  
Salma I. Abdalbagi ◽  
Maarouf I. Mohammed
Keyword(s):  
Regression Analysis ◽  
Sweet Sorghum ◽  
Cultural Practices ◽  
Ethanol Yield ◽  
Correlation And Regression Analysis ◽  
Juice Yield ◽  
Ethanol Yields ◽  
Significant Regression ◽  
Theoretical Ethanol Yield ◽  
Fermented Juice

An experiment was conducted in 2017 at Alwaha Project, Khartoum State to study the potential of ethanol yield from fermented juice of local and exotic sweet sorghums. 40 genotypes were arranged in RCB design. Juice yield, ethanol yield as percentage of fermented juice, actual and theoretical ethanol yields were studied. The Baker’s yeast (Saccharomyces cerevisiae) was used in the fermentation process. ANOVA tests, correlation and regression analysis of actual vs theoretical ethanol yield were performed. Significant differences were detected among genotypes for ethanol yields. High ethanol yields as percentage from fermented juice were obtained, but ethanol yield per hectare was relatively low due to low juice yields. The genotypes BlueRibbon and KensasCollies from exotic materials; 5AbjSG51 and S.154 Ab70 from local materials were identified as promising for ethanol production. Correlation between actual ethanol yield and Juice yield was positive and highly significant. Regression analysis indicated that theoretical ethanol yield was little bit overestimated. It was concluded that juice yield is more crucial than ethanol concentration in maximizing ethanol yield of sweet sorghum. More efforts are needed to develop high juice-yielding genotypes. Juice maximization can also be achieved by using efficient milling devices and optimizing cultural practices.

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