Detection of Cytomegalovirus by ELISA Technique in Rheumatoid Arthritis Patients in Mosul / Iraq

Expression of ICAM1 and VCAM1 Serum Levels in Rheumatoid Arthritis Clinical Activity. Association with Genetic Polymorphisms

Disease Markers ◽

10.1155/2009/510104 ◽

2009 ◽

Vol 26 (3) ◽

pp. 119-126 ◽

Cited By ~ 22

Author(s):

Rosa Elena Navarro-Hernández ◽

Edith Oregon-Romero ◽

Mónica Vázquez-Del Mercado ◽

Héctor Rangel-Villalobos ◽

Claudia Azucena Palafox-Sánchez ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Clinical Status ◽

Serum Levels ◽

Clinical Activity ◽

C Reactive Protein ◽

Soluble Adhesion Molecules ◽

Reactive Protein ◽

Erythrocyte Sedimentation ◽

Pcr Rflp ◽

Elisa Technique

To investigate the association of sICAM-1 and sVCAM-1 with ICAM1 721G>A and VCAM1 1238G>C polymorphisms and rheumatoid arthritis (RA) clinical activity, sixty RA patients and 60 healthy non-related subjects (HS) matched for age and sex were recruited. Soluble adhesion molecules were determined by ELISA technique. Rheumatoid factor (RF), C reactive protein (CRP) and the erythrocyte sedimentation rate (ESR) were measured by routine methods. Disability and clinical activity was measured with Spanish-HAQ-DI and DAS28 scores, respectively. The ICAM1 and VCAM1 polymorphism were identified using the PCR-RFLP procedure. Inter-group comparison showed increased levels of sICAM-1 and sVCAM-1 in RA patients (284 and 481 ng/mL) versus HS (132 and 280 ng/mL); in the RA group, significant correlations between sVCAM-1 and RF (r= 0.402), ESR (r= 0.426), Spanish-HAQ-DI (r= 0.276), and DAS28 (r= 0.342) were found, whereas sICAM-1 only correlated with RF (r= 0.445). In RA patients, a significant association with the 721A allele of ICAM1 polymorphism (p= 0.04), was found. In addition, the allele impact (G/A+A/A) of this polymorphism was confirmed, (p= 0.038, OR = 2.3, C.I. 1.1–5.0). sVCAM-1 and sICAM-1 serum levels reflected the clinical status in RA, independently of the ICAM1 and VCAM1 polymorphism. However, the ICAM1 721A allele could be a genetic marker to RA susceptibility.

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A Functional Haplotype of PADI4 Gene in Rheumatoid Arthritis: Positive Correlation in a French Population

The Journal of Rheumatology ◽

10.3899/jrheum.080398 ◽

2009 ◽

Vol 36 (5) ◽

pp. 881-886 ◽

Cited By ~ 16

Author(s):

FRÉDÉRIQUE GANDJBAKHCH ◽

ISABELLE FAJARDY ◽

BENJAMIN FERRÉ ◽

SYLVAIN DUBUCQUOI ◽

RENÉ-MARC FLIPO ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Direct Sequencing ◽

Susceptibility Gene ◽

Positive Association ◽

Arginine Deiminase ◽

Nucleotide Polymorphisms ◽

Exon 2 ◽

Asian Populations ◽

American College Of Rheumatology ◽

Elisa Technique

Objective.A functional haplotype of peptidyl arginine deiminase 4 (PADI4) was associated with susceptibility to rheumatoid arthritis (RA) in Asian populations, but the results are contradictory in Europeans. We investigated (1) the association of 2 single-nucleotide polymorphisms (SNP) located in exon 2 of PADI4 with RA in another Caucasian population; and (2) the association between PADI4 and anti-citrullinated protein (anti-CCP) antibodies.Methods.DNA samples were obtained from 405 French RA patients and 275 controls. All RA patients met the revised criteria of the American College of Rheumatology. PADI4_89 163(G→A) and PADI4_90 245(T→C) SNP were genotyped using a PCR-RFLP method confirmed by direct sequencing. All patients and controls were genotyped for HLA-DRB1. The presence of anti-CCP antibodies was tested in 243 RA patients using an ELISA technique.Results.We focused on PADI4_89 163(G→A) and PADI4_90 245(T→C) SNP that distinguished 2 main haplotypes: AC haplotype (PADI4_89*A PADI4_90*C) and GT haplotype (PADI4_89*G PADI4_90*T), described, respectively, as “nonsusceptible” and “susceptible.” A positive association between RA and presence of the GT haplotype was found in the heterozygous state (p = 0.002) and the homozygous state (RA patients 22%, controls 13%; p = 0.005). A correlation was observed between the presence but not the level of anti-CCP antibodies and the GT heterozygous (p = 0.03) and homozygous (p = 0.05) haplotypes. No correlation was found between the HLA-DRB1 shared epitope and any of the PADI4 haplotypes.Conclusion.Our findings confirm those of Japanese, Korean, and Canadian studies and suggest that PADI4 may be a new susceptibility gene independent of HLA-DRB1 for RA in Caucasian populations.

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Hyperbaric oxygen in animal model of rheumatoid arthritis: Analysis of HIF-1α, ACPA and IL-17a

Infectious Disease Reports ◽

10.4081/idr.2020.8766 ◽

2020 ◽

Author(s):

Titut Harnanik ◽

Sapta Prihartono ◽

Tedy Juliandhy

Keyword(s):

Rheumatoid Arthritis ◽

Animal Model ◽

Hyperbaric Oxygen ◽

Therapeutic Potential ◽

Hypoxia Inducible Factor ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Peptide Antibody ◽

Elisa Technique ◽

Different Doses

Antigen and collagen-induced arthritis (ACIA) is animal model of rheumatoid arthritis. The aim of this study was to identify the effect of different doses of hyperbaric oxygen (HBO) exposure in reducing inflammation on ACIA through analysis hypoxia inducible factor-1α (HIF-1α), anticyclic citrullinated peptide antibody (ACPA) and interleukine 17a (IL-17a). 24 male Balb/C mice were divided into 3 groups, 8 mice did not receive HBO exposure as a control group (G1) and 16 mice received HBO exposure as treatment group (G2 and G3). G2 was ACIA which was exposed to HBO 2.4 ATA O2 100% 90 minutes divided by 3 each 30 minutes intervals 2 times 5 minutes breathing with normal air for 10 consecutive days. G3 was ACIA which was exposed to HBO 2.4 ATA O2 100% 90 minutes divided by 3 each 30 minutes intervals 2 times 5 minutes breathing with normal air for 5 consecutive days, break 5 days, 5 consecutive days. ACPA and IL-17a were measured by enzyme-linked immunosorbent assay (ELISA) technique. The expression of HIF-1α was measured by immunohistochemistry technique. There was significant decrease of ACPA levels, IL-17a levels and HIF-1α expression (P< 0.05) in G2 and G3 compared to G1. There was not significant decrease of ACPA levels (P> 0.05), there was significant decrease of IL-17a levels and HIF-1α expression (P<0.05) in G2 compared to G3. G2 was a group with a higher oxygen partial pressure than G3. HBO 2.4 ATAO2 100% 90 minutes divided by 3 each 30 minutes intervals 2 times 5 minutes breathing with normal air for 10 consecutive days more effective in reducing inflammation than exposure 5 consecutive days, break 5 days, 5 consecutive days in ACIA. HBO has therapeutic potential for the treatment of RA.

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Association of TNFRII polymorphisms and IL-37 in rheumatoid arthritis Iraqi patients

Journal Port Science Research ◽

10.36371/port.2021.1.7 ◽

2021 ◽

Vol 4 (1) ◽

pp. 30-35

Author(s):

Wasnaa J. Mohammad ◽

Noor Alhuda Kh. Ibrahim ◽

Shahad F . Obed ◽

Mohammed Sh. Jebur

Keyword(s):

Rheumatoid Arthritis ◽

Gene Polymorphism ◽

Control Group ◽

Disease Development ◽

Healthy Individuals ◽

Pcr Rflp ◽

Elisa Technique

Fifty RA patients and 50 healthy individuals have been participated in this study. 196 M/R polymorphism of TNFRII gene determined by PCR-RFLP, IL-37 level was measured by ELISA technique, also RF, ACCP, CRP measured by ELISA technique. Our study shows an increase in IL-37 levels in patients was suffered from rheumatoid arthritis relative to control group. (M=101.31+10.41) That shows major differences between patients and controls (p<0.01) and increasing level of IL-37 correlate significantly with increasing level of CRP (p<0.05). The frequencies of TNFRII gene polymorphism were significantly correlate with the IL-37 level (p<0.01) in RA patients compared with control group. In conclusion, IL-37 increased in RA patients associated with disease development, also significantly associated with TNFRII polymorphism.

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