scholarly journals PHYSICOCHEMICAL AND PHYTOCHEMICAL ANALYSIS OF Mangifera indica linn.( AAMRA KERNEL )

2020 ◽  
Vol p4 (05) ◽  
pp. 2394-2400
Author(s):  
Pooja Dogra ◽  
A. Rama Murthy ◽  
Sumit Nathani
Keyword(s):  
Mangifera Indica ◽  
Natural Antioxidants ◽  
Water Soluble ◽  
Phytochemical Analysis ◽  
Tropical Regions ◽  
Potential Source ◽  
Mango Kernel ◽  
Phytochemical Constituents ◽  
Phytochemical Studies

Introduction: Mangifera Indica Linn., Anacardiaceae family, is a large evergreen tree of tropical and sub-tropical regions of the world. Mango kernel can be used as a potential source for functional food ingredi-ents, animal feeds, antimicrobial compounds and cosmetic due to its high quality of fat and protein as well as high levels of natural antioxidants. Aim: The aim of the present study was to evaluate the physicochemical and phytochemical analysis of Mangifera Indica Linn. Methods Aamra Kernel were collected, cleaned, dried and powdered. Aqueous and alcoholic extracts of Aamra kernel were analyzed for physicochemical and phytochemical constituents. The study was conduct-ed as per the guidelines of Ayurvedic pharmacopeia of India. Results: The result of physicochemical parameters (moisture content: 10.2%, total ash: 2.12%, acid insolu-ble ash: 0.3%, water soluble ash: 1.42%, aqueous extractive value: 14.67% and alcoholic extractive value 12.98%). The phytochemical studies revealed the presence of active constituents, carbohydrates, amino acid, steroids, proteins, saponins, alkaloids, glycosides, tannins in aqueous and alcoholic extracts. Conclusion: All studies standardization parameters like physicochemical and phytochemical screening pa-rameters provide the knowledge in the identification, authentication of kernel of Mangifera Indica Linn. Hence it is concluded that this drug supposed to be great beneficial and boon for our society.

Comparative Pharmacognostic, Physicochemical and Phytochemical Studies of Different Samples of Plumbago zeylanica L. Roots

2017 ◽  
Vol 9 (06) ◽  
Author(s):  
Neha Jain ◽  
Mohan Lal Kori
Keyword(s):  
Phytochemical Analysis ◽  
Local Market ◽  
Herbal Drugs ◽  
Disease Treatment ◽  
Organoleptic Evaluation ◽  
Plumbago Zeylanica ◽  
Qualitative And Quantitative ◽  
Crude Drugs ◽  
Phytochemical Studies

The objective of the present study is to evaluate the quality of the marketed and self collected samples of Plumbago zeylanica L. roots on the standardization parameters. This study is planned mainly to confirm changes with quality of drug. Now a day’s more demand of herbal drugs for disease treatment, lack of knowledge of proper methodology and availability are promoting the practices of adulteration and substitution. Thus, the standardization of the plant crude drugs is necessary to maintain their therapeutic efficacy. Comparative studies were carried out to evaluate the standards of P. zeylanica L. with emphasis on organoleptic evaluation, physicochemical and phytochemical analysis. Samples were procured from local market and self collected to determine the qualitative and quantitative variations. The result indicates that self collected sample showed significant results with comparison to marketed sample.

scholarly journals PHARMACOGNOSTICAL, PHYSICOCHEMICAL AND PHYTOCHEMICAL STUDIES OF PIPER LONGUM LINN. FRUITS

2019 ◽  
Vol 1 (1) ◽  
pp. 19-24
Author(s):  
Priyanka Sharma
Keyword(s):  
Chemical Constituents ◽  
Volatile Oil ◽  
Water Soluble ◽  
Phytochemical Analysis ◽  
Chemical Parameters ◽  
Piper Longum ◽  
The Family ◽  
Perennial Shrub ◽  
Physical And Chemical ◽  
Phytochemical Studies

Introduction: Piper longum Linn, belonging to the family Piperaceae, is a climber, perennial shrub which is commonly found in India.  The dried fruits of Piper longum are widely used in Ayurvedic System of medicines since time unknown. It posses significant pharmacological properties due to presence of variety of chemical constituents in it. Objective: The present study is aimed to evaluate the pharmacognostical, physiochemical and phytochemical parameters for Piper longum fruits, as per the WHO guidelines for herbal drug standardization. Materials and Methods: WHO emphasized the use of standardized herbs and formulations for safety and best therapeutic results. Results and Discussion: Pharmacognostical studies shows that the fruits of Piper longum are found as green when fresh which turns grayish black upon drying. It posses pungent, bitter and acrid taste. The fruits are cylindrical with small petiole. The powder microscopy shows the presence of brown content, oleo resins, stone cells and calcium oxalate crystals. Physiochemical analysis shows variable extractive values in different solvents with maximum extractives of 20.6±0.021% in water and minimum extractive value of 6.6±0.036% in chloroform. Total Ash value of 8.3±0.015%, Acid Insoluble Ash- 1.16±0.025% and Water Soluble Ash- 5.5±0.01%, Foreign Matter- 1.62±0.12%, Moisture content of 15.70±0.051%, Bitterness value-1.96, Swelling Index-1.5±0.01 and Foaming Index- 102.33. Phytochemical analysis of the fruits of Piper longum shows the presence of alkaloids, volatile oil, tannins, fixed oils, and phenolics. Conclusion: The botanical, physical and chemical parameters obtained in this study can be used for establishing the identity and purity of the drug that will lead to safety and efficacy of the herb.

scholarly journals Physicochemical Characterization, Phytochemical and HPTLC Fingerprinting Studies on Fruit of Couroupita Guianensis

2021 ◽  
Vol 20 (4) ◽  
Author(s):  
Lawrence Anna Sheba ◽  
◽  
Anuradha Venkatraman ◽  
Keyword(s):  
High Performance ◽  
Physicochemical Characterization ◽  
Water Soluble ◽  
Phytochemical Analysis ◽  
Important Criterion ◽  
Fruit Pulp ◽  
Primary Metabolites ◽  
Couroupita Guianensis ◽  
Medicinal Properties

Abstract Traditional knowledge and literature studies report that each part of a plant has tremendous medicinal values. Validation of these medicinal plants scientifically is an important criterion for the development of plant-based drugs. Couroupita guianensis (Family: Lecythidaceae) is a plant with immense medicinal properties. To authenticate its biological value, the present investigation aims to standardize the fruit of C. guianensis based on physicochemical characterization, phytochemical analysis both qualitatively and quantitatively, and high-performance thin-layer liquid chromatography (HPTLC) fingerprinting studies. Fruit pulp of C. guianensis was obtained, processed and extracted with solvents such as petroleum ether, chloroform, ethanol and hydroalcohol. Moisture content, total ash, water-soluble ash and acid-insoluble ash values were calculated. Preliminary phytochemical analysis revealed the existence of several secondary metabolites in the extracts. In addition, interpreting peaks obtained from HPTLC analysis revealed the presence of potential bioactive phytoconstituents in all the extracts. The quantitative determination proclaimed that fruit pulp was found to be rich in phenolics and flavonoids followed by tannin and saponin. Further, primary metabolites were quantified and they were found to be abundant in the fruit pulp. Henceforth, the outcome of these results provides information for assessing the quality of the sample that could help in ensuring its therapeutic efficacy. Keywords: Couroupita guianensis, HPTLC, Physicochemical characterization, Phytochemical, Fingerprinting

scholarly journals STANDARDIZATION AND TOXICOLOGICAL STUDIES OF THE LEAF OF SENNA SIAMEA IRWIN & BARNEBY (FABACEAE) COLLECTED FROM AGULU IN AWKA SOUTH OF ANAMBRA STATE

2021 ◽  
Vol 3 (01) ◽  
pp. 25-36
Author(s):  
Chinasa Charity Ezea
Keyword(s):  
Methanol Extract ◽  
Proximate Analysis ◽  
Water Soluble ◽  
Control Group ◽  
Phytochemical Analysis ◽  
Standard Methods ◽  
Toxicological Effect ◽  
Toxicological Effects ◽  
Toxicological Studies ◽  
Phytochemical Constituents

Background: Pharmacognostic standards and toxicological studies of the leaf of S. siamea were carried out. Objectives: To determine the quantitative phytochemical constituents present, pharmacognostic standards and  toxicological effect of the methanol leaf extract and fractions of S. siamea. Methodology: Fresh leaves of S. siamea were collected, dried, pulverizes to powder and subjected to pharmacognostic and quantitative phytochemical analysis. The pulverized leaves were cold macerated in methanol for three days with intermittent shaking and exchange of fresh solvent every 24 hrs (aliquot extraction). Fractionation was done using n-hexane, ethylacetate and butanol. Chromatographic analysis of the methanol extract and fractions were obtained using standard methods. The methanol extract and fractions where screened for phytochemical constituents and toxicological effects using standard methods. Acute toxicity (LD50) of the extract was determined  using Lorke’s method. Results: Macroscopic and microscopic examinatios revealed acute apex, pinnate veination and diacytic stomata, trichomes respectively. Phytochemical screening of the extract and fractions revealed the presence of flavonoids, alkaloids, saponins, tannins etc. Proximate analysis of S. siamea revealed moisture content of 5.50 %, total ash value of 15.00 %, water soluble ash of 2.00 % etc.  The LD50 was found to be > 5000 mg/kg. Increase in ALT, AST and ALP when metanol extract and fractions were administered were not statistically different from the control group, indicating no hepatotoxic potential. Increase in PCV, Hb and RBC when metanol extract and fractions were administered were not statistically different from control group. The non-significant decrease in the levels of ALP, ALT and AST indicates that the extract has possible hepatoprotective effect. The non-significant decrease in the levels of RBC, Hb and PCV indicates that the extract does not affect the heamatopoietic system when administered orally and at the doses used in the study. Conclusions: Specific standards obtained from the study may be compiled into a monograph of S.siemea plant that may constitute in herbal pharmacopoeia.

scholarly journals Physicochemical and Phytochemical Studies on Sphaeranthus indicus Linn. with HPTLC Fingerprinting

2021 ◽  
Vol 11 (2) ◽  
pp. 100-107
Author(s):  
Mohit Garg ◽  
Neelesh Dwivedi
Keyword(s):  
Water Soluble ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Ph Values ◽  
Natural Agents ◽  
Sphaeranthus Indicus ◽  
Health And Healing ◽  
Therapeutic Compounds ◽  
Phytochemical Studies

Ayurveda, the oldest paradigm of health and healing known to the world, grew out of the Rishi tradition. The medical and research community is constantly seeking for new natural agents. Sphaeranthus indicus Linn. (Gorakhmundi, Family: Asteraceae), is widely used in Ayurvedic system of medicine to treat various diseases. The present investigation was designed to perform physicochemical and phytochemical analysis with HPTLC fingerprints of Sphaeranthus indicus (leave, flower, and stem) to establish the standard parameters of this herb. Different analytical parameters like extractive values, total ash, acid insoluble ash and water-soluble ash, moisture content, pH values of different parts of the drug were performed. Preliminary phytochemical screening was done to detect and quantify different active constituents such as alkaloids, flavanoids, proteins and total poly phenol using various analytical methods. Our preliminary data of phytochemical screening of the extracts revealed the presence of steroids, carbohydrates, proteins, terpenoid, flavonoid, tannins, alkaloids, resin and saponin. In the current study we established the HPTLC fingerprint of the extract using phytochemical standards. We believe that our study provides a substantial data for identification, purification, standardization, and phytochemical characterization of novel therapeutic compounds that can be used potential drug to combat various diseases for betterment of mankind. Keywords: Sphaeranthus indicus, Ayurveda, physicochemical, UV- Spectrophotometer, HPTLC

scholarly journals Phytochemistry, chelating and reducing potential of Mesosphaerum suaveolens (L.) Kuntze (Lamiaceae) essential oil

2020 ◽  
Vol 9 (11) ◽  
pp. e1099119333
Author(s):  
Elvis Estilak Lima ◽  
Adrielle Rodrigues Costa ◽  
José Weverton Almeida-Bezerra ◽  
Viviane Bezerra da Silva ◽  
Saulo Almeida de Menezes ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Essential Oil ◽  
Chromatographic Analysis ◽  
Reducing Power ◽  
Natural Antioxidants ◽  
Cellular Damage ◽  
Phytochemical Analysis ◽  
Chelating Activity ◽  
Potential Source ◽  
Reducing Potential

Products of natural origin with potential chelating or reducing Fe+ free become promising in antioxidant research, because they have the ability to soften transition metals that can cause cellular damage by oxidative stress. Thus, the aim of this study is to promote the evaluation of the antioxidant potential of essential oil by the orthophenanthroline method, in addition to promoting phytochemical analysis of the essential oil of M. suaveolens. This vegetable, by other antioxidant methods, has promising properties in inhibiting free radicals. To achieve these objectives, a chromatographic analysis was performed, as well as the orthophenanthroline assays to evaluate the formation of the Fe2+ 1,10-orthophenanthroline complex. The β-Caryophyllene (20.38%) stands out as the major compound of the species. The essential oil interfered with the formation of the complex, suggesting a moderate chelating activity at the highest concentration tested (480 µg/mL), demonstrating that the oil can chelate Fe2+. The addition of ascorbic acid to the mixture significantly increased the absorbance for all tested concentrations, in the evaluation of the reducing power from Fe3+ to Fe2+. Considering a potential source of natural antioxidants, it may be relevant for the management of oxidative stress.

Feminine intimate hygiene plants from Kinshasa / DR Congo: Potential source of contraceptive compounds

2021 ◽  
Vol 1 (1) ◽  
pp. 071-078
Author(s):  
Odette N Kabena ◽  
Jean Jacques D Amogu ◽  
Nadège K Ngombe ◽  
EJ Mboloko ◽  
Pius T Mpiana ◽  
...  
Keyword(s):  
Mangifera Indica ◽  
Human Sperm ◽  
Phytochemical Analysis ◽  
Dr Congo ◽  
Ethnobotanical Survey ◽  
Bridelia Ferruginea ◽  
Contraceptive Effect ◽  
Potential Source ◽  
Sperm Mobility ◽  
The One

This study is part of the promotion of local medicinal plants by highlighting their spermicidal activity. An ethnobotanical survey carried out in Kinshasa made it possible to identify the plants used in female intimate hygiene. On the one hand, the aqueous extracts of the organs of ten (10) plants on the inventoried species were subjected to a phytochemical analysis. On the other hand, the recipes as applied by women as well as the total extracts of these plants have come into contact with human sperm. The analysis of the mobility and the evaluation of the viability of the sperm of the ejaculates, of good quality according to the WHO, were carried out according to the methods of Sander-Cramer and that of Williams, respectively. Thus, the concentration (EC100) capable of killing 100% of sperm within 30 minutes of exposure was detected. Thirty-eight (38) plant species have been identified. From thirteen sought-after phytochemical groups, extracts of Mangifera indica and Bridelia ferruginea contain twelve and eleven respectively. Four (4) groups of metabolic compounds with a contraceptive effect are present in all ten extracts of feminine hygiene plants. Three (3) herbal recipes (Abrus precatorius, Bridelia ferruginea and Ocimum basilicum) out of the ten studied resulted in the cancellation of sperm mobility after 3 hours of exposure. And, the EC100 is defined at 200 mg/mL for A. precatorius and 25 mg / mL for B. ferruginea. The results of this study show that feminine intimate hygiene plants contain compounds with spermicidal potential, which can be used for the development of vaginal contraceptives.

scholarly journals A STUDY ON PHYTOCHEMICALS, ANTIMICROBIAL, AND SYNERGISTIC ANTIMICROBIAL ACTIVITIES OF HIBISCUS SABDARIFFA

2019 ◽  
pp. 198-201
Author(s):  
SIVAN KUMAR ◽  
ANNA SHEBA L
Keyword(s):  
Leaf Extract ◽  
Antimicrobial Properties ◽  
Antimicrobial Activities ◽  
Phytochemical Analysis ◽  
Synergistic Activity ◽  
Hibiscus Sabdariffa ◽  
Bioactive Components ◽  
Potential Source ◽  
Phytochemical Constituents ◽  
Diffusion Assay

Objective: The aim of the present study was to screen phytochemical constituents and evaluate antimicrobial and synergistic antimicrobial properties of leaves and stem of Hibiscus sabdariffa. Methods: The extraction was done by cold maceration method using 80% aqueous methanol. The antimicrobial efficacy and synergistic antimicrobial activity were carried out by disc diffusion assay against the bacteria Staphylococcus aureus and Pseudomonas aeruginosa. Results: Phytochemical analysis revealed the presence of carbohydrate, protein, alkaloids, phytosterols, flavonoids, and diterpenes in both the leaves and stem extracts while saponins, phenol, and tannins were found to be present only in the leaf extract. Both the extracts inhibited the tested bacteria with minimum inhibitory concentration value of 10 mg/ml. Aqueous methanolic extract of leaf showed higher antibacterial activity against S. aureus and P. aeruginosa and also exhibited synergistic activity with the antibiotic chloramphenicol against S. aureus. Conclusion: The present study concludes that H. sabdariffa is a potential source of bioactive components and also provides information on synergistic activity of leaf extract. The results can contribute to the development of potent antibacterial agents.

Use of water-soluble polysaccharides from mango (Mangifera indica L.) fruits as alternative source of mutanase inductors

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
A Wiater ◽  
K Próchniak ◽  
M Janczarek ◽  
M Pleszczyńska ◽  
M Tomczyk ◽  
...  
Keyword(s):  
Mangifera Indica ◽  
Water Soluble ◽  
Alternative Source

Pharmacognostical fingerprinting and selective bioactivity studies of Solanum glaucophyllum Desf.

2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Anindya Sundar Ray ◽  
Suman Kalyan Mandal ◽  
Chowdhury Habibur Rahaman
Keyword(s):  
Medicinal Plant ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Phytochemical Analysis ◽  
Antimicrobial Studies ◽  
Maximum Inhibition ◽  
Crude Drugs ◽  
Phytochemical Studies ◽  
Dpph Radical Scavenging

Solanum glaucophyllum Desf. (Solanaceae) is traditionally used for curing several health conditions in both human and domesticated animals. There is no data available regarding pharmacognostic standardization and pharmacological activity of this less known medicinal plant. Therefore, the aim of the present work is to prepare pharmacognostic fingerprints of crude drugs obtained from the leaf and stem parts of S. glaucophyllum. Apart from this detailed phytochemical analysis, antioxidant and antimicrobial studies have also been carried out. Pharmacognostic study revealed that the leaves are amphistomatic and stomata are strictly of anisocytic type. Values of stomatal index, palisade ratio, ash content of the investigated parts are found distinct and can be used as pharmacognostic standards for evaluation of crude drugs of this medicinal plant. Phytochemical studies of the leaf and stem parts indicated that phenolics, flavonoids, tannins and alkaloids are present in quite impressible amount. Curcumin content in stem was found very high through HPLC analysis. In DPPH radical scavenging assay, the stem part showed significant antioxidant potential. Ethanol extract of stem produced maximum inhibition zone (11mm) against Pseudomonas aeruginosa at the concentration of 50 mg/ml among five test microorganisms. Some pharmacognostic features recorded in this study as diagnostic ones will help in proper identification of this ethnomedicinal plant in its fresh as well as dried form. On the other hand preliminary phytochemical, antioxidant and antimicrobial studies highlight the S. glaucophyllum as a promising candidate for bioprospecting.

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