Toll-like receptor 3 signaling and human glomerular endothelial cells

2020 ◽  
Vol 1 (1) ◽  
Keyword(s):  
Endothelial Cells ◽  
Toll Like Receptor ◽  
Glomerular Endothelial Cells ◽  
Human Glomerular Endothelial Cells

scholarly journals Cytosolic Sensors of Viral RNA Are Involved in the Production of Interleukin-6 via Toll-Like Receptor 3 Signaling in Human Glomerular Endothelial Cells

2019 ◽  
Vol 44 (1) ◽  
pp. 62-71 ◽  
Author(s):  
Qiang Liu ◽  
Tadaatsu Imaizumi ◽  
Tomomi Aizawa ◽  
Koji Hirono ◽  
Shogo Kawaguchi ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Signaling Pathway ◽  
Interleukin 6 ◽  
Viral Rna ◽  
Pivotal Role ◽  
Toll Like Receptor ◽  
Polycytidylic Acid ◽  
Glomerular Endothelial Cells ◽  
Human Glomerular Endothelial Cells ◽  
Tlr3 Signaling

Background/Aims: Dysregulation of interleukin-6 (IL-6) production in residual renal cells may play a pivotal role in the development of glomerulonephritis (GN). Given that Toll-like receptor 3 (TLR3) signaling has been implicated in the pathogenesis of some forms of GN, we examined activated TLR3-mediated IL-6 signaling in cultured normal human glomerular endothelial cells (GECs). Methods: We treated GECs with polyinosinic-polycytidylic acid (poly IC), an authentic double-stranded RNA, and analyzed the expression of IL-6 and the cytosolic viral RNA sensors retinoic acid-inducible gene-I (RIG-I) and melanoma differentiation associated gene 5 (MDA5) using reverse transcription quantitative real-time polymerase chain reaction, western blotting, and enzyme-linked immunosorbent assays. To further elucidate the effects of poly IC on this signaling pathway, we subjected the cells to small interfering RNA (siRNA) against TLR3, interferon (IFN)-β, RIG-I, and MDA5. Results: We found that poly IC induced the expression of RIG-I, MDA5 and IL-6 via TLR3/IFN-β signaling in GECs. siRNA experiments revealed that both MDA5 and RIG-I were involved in the poly IC-induced expression of IL-6, with MDA5 being upstream of RIG-I. Conclusion: Interestingly, cytosolic sensors of viral RNA were found to be involved in IL-6 production via TLR3 signaling in GECs. Regional activation of TLR3/IFN-β/ MDA5/RIG-I/IL-6 axis due to viral and “pseudoviral” infections is involved in innate immunity and inflammatory reactions in GECs. We believe this signaling pathway also plays a pivotal role in the development of some forms of GN.

scholarly journals IFIT Proteins Are Involved in CXCL10 Expression in Human Glomerular Endothelial Cells Treated with a Toll-Like Receptor 3 Agonist

2020 ◽  
pp. 1-12
Author(s):  
Tadaatsu Imaizumi ◽  
Shun Hashimoto ◽  
Riko Sato ◽  
Hidenori Umetsu ◽  
Tomomi Aizawa ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Innate Immune ◽  
Toll Like Receptor ◽  
Tetratricopeptide Repeats ◽  
Immune Reactions ◽  
Polycytidylic Acid ◽  
Glomerular Endothelial Cells ◽  
Chemokine Ligand ◽  
Human Glomerular Endothelial Cells ◽  
Novel Coronavirus

Introduction: Various viruses including a novel coronavirus (SARS-CoV-2) can infect the kidney. When viruses invade the glomeruli from the bloodstream, glomerular endothelial cells (GECs) initiate the innate immune reactions. We investigated the expression of interferon (IFN)-induced protein with tetratricopeptide repeats (IFIT) 1/2/3, antiviral molecules, in human GECs treated with a toll-like receptor (TLR) 3 agonist. Role of IFIT1/2/3 in the expression of C-X-C motif chemokine ligand 10 (CXCL10) was also examined. Methods: Human GECs were cultured and stimulated with polyinosinic-polycytidylic acid (poly IC), a synthetic TLR3 agonist. Real-time qPCR, Western blotting, and ELISA were used to examine the expression of IFIT1/2/3, IFN-β, and CXCL10. RNA interference against IFN-β or IFIT1/2/3 was also performed. Results: Expression of IFIT1/2/3 and CXCL10 was induced by poly IC in GECs. The inductions were inhibited by RNA interfering of IFN-β. Knockdown of IFIT1/2/3 decreased the CXCL10 expression. Knockdown of IFIT3 decreased the expression of IFIT1 and IFIT2 proteins. Conclusion: IFIT1/2/3 and CXCL10 were induced by poly IC via IFN-β in GECs. IFIT1/2/3 may increase the expression of CXCL10 which induces lymphocyte chemotaxis and may inhibit the replication of infected viruses. These molecules may play a role in GEC innate immune reactions in response to viruses.

Endothelial expression of fractalkine (CX3CL1) is induced by Toll-like receptor 3 signaling in cultured human glomerular endothelial cells

2019 ◽  
Vol 30 (6) ◽  
pp. 1074-1081
Author(s):  
Koji Hirono ◽  
Tadaatsu Imaizumi ◽  
Tomomi Aizawa ◽  
Shojiro Watanabe ◽  
Koji Tsugawa ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Toll Like Receptor ◽  
Glomerular Endothelial Cells ◽  
Human Glomerular Endothelial Cells

Toll-Like Receptor 3 Signaling Contributes to Regional Neutrophil Recruitment in Cultured Human Glomerular Endothelial Cells

Nephron ◽  
2018 ◽  
Vol 139 (4) ◽  
pp. 349-358 ◽  
Author(s):  
Qiang Liu ◽  
Tadaatsu Imaizumi ◽  
Shogo Kawaguchi ◽  
Tomomi Aizawa ◽  
Tomoh Matsumiya ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Neutrophil Recruitment ◽  
Toll Like Receptor ◽  
Glomerular Endothelial Cells ◽  
Human Glomerular Endothelial Cells

Activation of general control nonderepressible 2 kinase protects human glomerular endothelial cells from harmful high-glucose-induced molecular pathways

2016 ◽  
Vol 48 (10) ◽  
pp. 1731-1739 ◽  
Author(s):  
Theodoros Eleftheriadis ◽  
Konstantina Tsogka ◽  
Georgios Pissas ◽  
Georgia Antoniadi ◽  
Vassilios Liakopoulos ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
High Glucose ◽  
Molecular Pathways ◽  
General Control ◽  
Glomerular Endothelial Cells ◽  
Human Glomerular Endothelial Cells

scholarly journals Double-Stranded DNA Activates Glomerular Endothelial Cells and Enhances Albumin Permeability via a Toll-Like Receptor-Independent Cytosolic DNA Recognition Pathway

2009 ◽  
Vol 175 (5) ◽  
pp. 1896-1904 ◽  
Author(s):  
Holger Hägele ◽  
Ramanjaneyulu Allam ◽  
Rahul D. Pawar ◽  
Christoph A. Reichel ◽  
Fritz Krombach ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Dna Recognition ◽  
Toll Like Receptor ◽  
Double Stranded Dna ◽  
Glomerular Endothelial Cells

Modulation of radiation‐induced damage of human glomerular endothelial cells by SMPDL3B

2020 ◽  
Vol 34 (6) ◽  
pp. 7915-7926
Author(s):  
Alaa Abou Daher ◽  
Marina Francis ◽  
Patrick Azzam ◽  
Anis Ahmad ◽  
Assaad A. Eid ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Glomerular Endothelial Cells ◽  
Radiation Induced ◽  
Human Glomerular Endothelial Cells

scholarly journals Conditionally immortalized human glomerular endothelial cells expressing fenestrations in response to VEGF

2006 ◽  
Vol 69 (9) ◽  
pp. 1633-1640 ◽  
Author(s):  
S.C. Satchell ◽  
C.H. Tasman ◽  
A. Singh ◽  
L. Ni ◽  
J. Geelen ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Glomerular Endothelial Cells ◽  
Human Glomerular Endothelial Cells
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