scholarly journals Prevalence of Listeria monocytogenes and other Listeria spp. in gulls feeding on Zagreb landfill site, Croatia

2021 ◽  
Vol 53 (4) ◽  
Author(s):  
Andrea Humski ◽  
Luka Jurinović ◽  
Tajana Amšel Zelenika ◽  
Biljana Ječmenica ◽  
Fani Krstulović ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Wild Birds ◽  
Landfill Site ◽  
First Results ◽  
Larus Michahellis ◽  
Conventional Methods ◽  
Prevalent Species

Gulls, as migratory wild birds are known that can spread different pathogens over long distances. Aim of this study was to access prevalence of different Listeria species in their population fed at the Zagreb landfill site. Altogether, 390 gulls of three species were sampled: Yellow-legged Gull, Larus michahellis; Black-headed Gull, L. ridibundus and Common Gull, L. canus. Most prevalent species was L. innocua (14.4%), while L. monocytogenes was found in 11.3 % of tested samples. The presence of other species was found in a smaller percentage, namely L. welshimeri (1.3%), L. ivanovii (0.5%) and L. seeligeri (0.3%). Serotyping of L. monocytogenes isolates was performed using both molecular and conventional methods, and most isolates belonged to serotypes 1/2a and 1/2b. To the best of our knowledge, this study reports the first results of research on the presence of L. monocytogenes and other Listeria spp. in wild birds in Croatia.

scholarly journals ISOLATION OF LISTERIA MONOCYTOGENES FROM FRESH FRUITS AND VEGETABLES

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 628b-628 ◽  
Author(s):  
Rehana Vahidy ◽  
Farrukh Jahan ◽  
Rizwana Nasim
Keyword(s):  
Public Health ◽  
Listeria Monocytogenes ◽  
Fruits And Vegetables ◽  
Serological Tests ◽  
Two Samples ◽  
Veterinary Importance ◽  
Conventional Methods ◽  
Water Melon

Listeriosis, a fatal food and environment borne disease of public health and veterinary importance, caused by Listeria spp. has not been explored in Pakistan. One hundred and fifty samples of fresh fruits and vegetables collected over a period of 12 months from various localities of Karachi city were screened according to the standard protocols. Suspected isolates were subjected to conventional methods for identification which included morphological, cultural, biochemical and serological tests. Out of 30 samples each of papaya, water melon and cantaloupe, and 15 each of cucumber, tomato, radish and carrot, Listeria monocytogenes was isolated from two samples of papaya and tomato each and one sample of water melon and cucumber each. It is inferred that fruits and vegetables, if not handled hygienically and served fresh, may be responsible for the transmission of listeriosis.

scholarly journals VOLUME ESTIMATION OF FUEL LOAD FOR HAZARD REDUCTION BURNING: FIRST RESULTS TO A VOXEL APPROACH

2020 ◽  
Vol XLIII-B3-2020 ◽  
pp. 1199-1206
Author(s):  
M. S. R. S. Eusuf ◽  
J. Barton ◽  
B. Gorte ◽  
S. Zlatanova
Keyword(s):  
Global Warming ◽  
Land Management ◽  
Management Practices ◽  
Volume Estimation ◽  
Fuel Load ◽  
Hazard Reduction ◽  
Fuel Loads ◽  
First Results ◽  
Conventional Methods

Abstract. In 2019/20 over 100 severe bushfires burned across the continent of Australia. The severity of these fires was exacerbated by many factors, including macroclimatic effects of global warming and, at the meso and micro scales, land management practices. The bushfire phenomenon cannot be stopped, however better management practices can help counter the increasing severity of fires. Hazard reduction burning is a method where certain vegetation is deliberately burned under controlled circumstances to thin the fuel to reduce the severity of bushfires. Fuel load is an important parameter to assess when hazard reduction burning, as the accumulation of vegetation in a forest profile affects the intensity of the burn. Conventional methods of measuring fuel load are time consuming and costly, and therefore it becomes increasingly important to investigate automated approaches for assessing fuel loads. This paper provides an overview of hazard reduction burning while explaining the methods to quantify fuel load. Then the paper presents our voxel approach in estimating the volume of fuel loads. The first results regarding different voxel resolutions are reported and analysed. This paper concludes with future steps and developments.

First results on the detection of Listeria monocytogenes in raw milk: comparison of two different analytical methods

2009 ◽  
Vol 33 (S1) ◽  
pp. 253-255
Author(s):  
A. Paris ◽  
E. Riboldi ◽  
F. Salmi ◽  
C. Bacci ◽  
S. Bonardi ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Analytical Methods ◽  
Raw Milk ◽  
First Results

scholarly journals Screening of crows and waterfowls for Salmonella and Listeria monocytogenes infection

2019 ◽  
pp. 9-19
Author(s):  
Hanaa Mohamed Fadel ◽  
Amira Ahmed
Keyword(s):  
Listeria Monocytogenes ◽  
Wild Birds ◽  
Pcr Detection ◽  
Direct Pcr ◽  
Step Method ◽  
Intervention Measures ◽  
Animal Pathogens ◽  
Significant Difference ◽  
Spread Of Infection ◽  
Resistance Rates

Background and Aim: Wild birds can carry a number of potential human and animal pathogens. These birds can intrude into human habitats giving the opportunity to transmission of such infection. Therefore, the current study was designed to investigate the role of crows and waterfowls as vectors of Salmonella and Listeria monocytogenes and to compare cultivation methods with direct polymerase chain reaction (PCR) for the detection of infection. Materials and Methods: A total of 200 fecal dropping samples were collected. Salmonella was cultivated by three step method (Universal Pre-enrichment Broth [UPB], Rappaport-Vassiliadis Broth, and Xylose Lysine Desoxycholate agar). The recovered isolates were characterized by biotyping, serotyping, and PCR detection of enterotoxin (stn) gene. The antibiogram pattern of isolates against a panel of 8 antibiotics was recorded. L. monocytogenes was cultivated on UPB, then on Listeria Oxford Agar and Listeria CHROMagar. The recovered isolates were characterized by biotyping and PCR detection of listeriolysin (hylA) gene. Results: The percentages of Salmonella infection in crows and waterfowls were 10 and 20, respectively. The most frequently recovered serovars were Typhimurium, Potengi, Enteritidis, and Kentucky. Antimicrobial susceptibility analysis of Salmonella isolates showed that the resistance rates for gentamicin were the highest (92%), followed by amoxicillin (88%) and cefixime (60%). Resistance to 8 antibiotics was recorded in 60% (15/25) of Salmonella isolates. The percentages of L. monocytogenes infection in crows and waterfowls were 1.3 and 2, respectively. Direct PCR applied to UPB revealed that 12% and 3% of samples were positive for Salmonella and L. monocytogenes, respectively, and there was no significant difference between direct PCR and cultivation method for the detection of infection. Conclusion: The present findings indicate that wild birds can harbor zoonotic enteric pathogens and this necessitates monitoring the epidemiologic status of these pathogens among wild birds and humans and applying the appropriate intervention measures to prevent the spread of infection.

Comparison of Oxygen Scavengers for Their Ability to Enhance Resuscitation of Heat-injured Listeria monocytogenes

1995 ◽  
Vol 58 (3) ◽  
pp. 244-250 ◽  
Author(s):  
J. R. PATEL ◽  
C.-A. HWANG ◽  
L. R. BEUCHAT ◽  
M. P. DOYLE ◽  
R. E. BRACKETT
Keyword(s):  
Listeria Monocytogenes ◽  
Incubation Time ◽  
Skim Milk ◽  
Oxygen Scavengers ◽  
Sodium Pyruvate ◽  
Percentage Recovery ◽  
Cysteine Hydrochloride ◽  
Whole Milk ◽  
Conventional Methods ◽  
Optimal Incubation

The recovery of heat-injured Listeria monocytogenes Scott A in Fraser broth (FB) supplemented with sodium thioglycolate, sodium pyruvate, L-(+)-cysteine hydrochloride, catalase or Oxyrase? was studied. After 3 h of incubation at 30°C, recovery was enhanced by all oxygen scavengers except sodium pyruvate. Oxyrase? (0.005 U ml−1) promoted the highest recovery (34%) compared to recovery in control broth (19%). All oxygen scavengers enhanced the recovery of injured L. monocytogenes in FB within 6 h of incubation. After 6 h at 30°C, 49 and 55% of injured cells underwent resuscitation in FB containing 2.5 mg of sodium pyruvate ml−1 and 400 μg of catalase ml−1, respectively, compared to 24% resuscitation in FB not supplemented with oxygen scavengers. The percentage recovery was increased as the incubation time was extended to 6 and 24 h. Nearly all injured cells were recovered within 24 h of incubation, regardless of supplementation of FB with oxygen scavengers. Fraser broth containing 2.5 mg of sodium pyruvate ml−1, 400 μg of catalase ml−1 or 0.01 U of Oxyrase? ml−1 were tested to determine the optimal incubation time and temperature for recovering heat-injured L. monocytogenes. Percentage recovery of injured cells increased with an increase in temperature from 25 to 30°C and from 30 to 35°C. The highest percentage of injured cells recovered was observed in FB containing 400 μg of catalase ml−1 (67%) and 0.01 U of Oxyrase? ml−1 (68%) within 6 h of incubation at 35°C. Catalase (400 μg ml−1) and Oxyrase? (0.01 U ml−1) in FB resulted in significantly higher recovery of injured cells from heated whole milk; however, recovery of injured cells from heated skim milk was not significantly higher. Enrichment in FB containing catalase or Oxyrase? has potential for recovering heat-injured L. monocytogenes cells within 6 h compared to 24 h required in conventional methods.

scholarly journals Treatment of extremity fractures in 20 wild birds with a modified Meynard external fixator and clinical assessment of the results

2021 ◽  
Author(s):  
K Gerbaga Ozsemir ◽  
K Altunatmaz
Keyword(s):  
External Fixator ◽  
Femur Fracture ◽  
Wild Birds ◽  
External Fixators ◽  
White Storks ◽  
Ulna Fractures ◽  
Larus Michahellis ◽  
Humerus Fractures ◽  
Imperial Eagle ◽  
Aquila Heliaca

The aim of this study is to evaluate the outcome of the treatment on twenty wild birds presenting with fractures that were stabilised with modified Meynard external fixators. The study material consisted of a total of twenty birds of eight different species, six white storks (Ciconia ciconia), one eastern imperial eagle (Aquila heliaca), one European honey buzzard (Pernis apivorus), one long-legged buzzard (Buteo rufinus), three common buzzard (Buteo buteo), one northern goshawk (Accipiter gentilis), one Armenian gull (Larus armenicus), and six yellow-legged gulls (Larus michahellis), which were diagnosed with fractures in their extremity bones following clinical and radiological examinations. The lesions of the twenty cases evaluated in the study were diagnosed as humerus fractures in nine cases, ulna fractures in three cases, tibiotarsus fractures in seven cases and a femur fracture in one case. The external fixators were removed between weeks five and nine postoperatively. While healing was seen in twelve of the twenty evaluated cases, the complete functional recovery occurred in six of these and they were released into nature. It was concluded that modified Meynard external fixator, which was made applicable for fractures in birds, could be an alternative method for fracture treatments in wild birds.

scholarly journals Isolation and Characterization of Listeria monocytogenes Isolates from Ready-To-Eat Foods in Florida

2006 ◽  
Vol 72 (7) ◽  
pp. 5073-5076 ◽  
Author(s):  
Yuelian Shen ◽  
Yan Liu ◽  
Yifan Zhang ◽  
Jennifer Cripe ◽  
William Conway ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Listeria Monocytogenes ◽  
Antibiotic Susceptibility ◽  
Food Samples ◽  
Multiple Antibiotic Resistance ◽  
Isolation And Characterization ◽  
Lineage 2 ◽  
Conventional Methods ◽  
Specific Bacteriophage

ABSTRACT Of 3,063 ready-to-eat food samples tested, 91 (2.97%) were positive for Listeria monocytogenes, and lineage 1 strains outnumbered lineage 2 strains 57 to 34. Seventy-one isolates (78%) exhibited multiple antibiotic resistance, and an L. monocytogenes-specific bacteriophage cocktail lysed 65 of 91 (71%) isolates. Determining phage, acid, and antibiotic susceptibility phenotypes enabled us to identify differences among strains which were otherwise indistinguishable by conventional methods.

scholarly journals PRESENCE OF Listeria monocytogenes IN HEALTHY CATTLE AT SLAUGHTERING: FIRST RESULTS

2010 ◽  
Vol 1 (7) ◽  
pp. 21
Author(s):  
G. Colavita ◽  
L. Vercellotti ◽  
E. Pavoletti ◽  
F. Chiesa ◽  
D. De Palma ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Healthy Cattle ◽  
First Results
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