scholarly journals Effects of surfactants on the remediation of petroleum contaminated soil and surface hydrophobicity of petroleum hydrocarbon degrading flora

2020 ◽  
Vol 26 (5) ◽  
pp. 200384-0
Author(s):  
Jianbo Liu ◽  
Liming Xu ◽  
Feifei Zhu ◽  
Shouhao Jia
Keyword(s):  
Contaminated Soil ◽  
Petroleum Hydrocarbons ◽  
Petroleum Hydrocarbon ◽  
Initial Conditions ◽  
Removal Rate ◽  
Surface Hydrophobicity ◽  
Orthogonal Experiments ◽  
Petroleum Contaminated Soil ◽  
Application Potential ◽  
High Removal Rate

It has been proven that surfactants used in the remediation of petroleum hydrocarbon contaminated soil have great application potential. In this study, the effects of five surfactants (SDBS, Tween80, Tween60, rhamnolipid and TRS-1) on leaching of petroleum hydrocarbons from soil were investigated through orthogonal experiments, and petroleum hydrocarbon components were analyzed by GC/MS. The effects of surfactants on the degradation of petroleum hydrocarbon were analyzed by the changes of microbial growth curve and surface hydrophobicity. The results showed that surfactant type, temperature and surfactant concentration had significant effects on the removal rate of petroleum hydrocarbon. Tween80, rhamnolipid and TRS-1 have good bio-friendliness and a high removal rate of petroleum hydrocarbons (up to 65%), suitable for the restoration of the soil used in the experiment And Surfactants exhibited a higher removal rate for small molecules and petroleum hydrocarbons with odd carbon atoms. Surfactants have a certain modification effect on the surface of relatively hydrophilic bacteria under the initial conditions, making their surface properties develop in the direction of enhanced hydrophobicity, and the hydrophobicity has increased from less than 20% to about 40%.

scholarly journals Soil Microcosms for Bioaugmentation With Fungal Isolates to Remediate Petroleum Hydrocarbon-contaminated Soil

2021 ◽  
Author(s):  
Dalel Daâssi ◽  
Fatimah Qabil Almaghribi
Keyword(s):  
Contaminated Soil ◽  
Petroleum Hydrocarbons ◽  
Fungal Growth ◽  
Total Petroleum Hydrocarbons ◽  
Fungal Culture ◽  
Soil Microcosms ◽  
Fungal Isolates ◽  
Petroleum Contaminated Soil ◽  
Decaying Wood ◽  
Native Strains

Abstract The aim of this work was to isolate indigenous PAH degrading-fungi from petroleum contaminated soil and exogenous ligninolytic strains from decaying-wood, with the ability to secrete diverse enzyme activity. A total of ten ligninolytic fungal isolates and two native strains, has been successfully isolated, screened and identified. The phylogenetic analysis revealed that the indigenous fungi (KBR1 and KB8) belong to the genus Aspergillus niger and tubingensis. While the ligninolytic exogenous PAH-degrading strains namely KBR1-1, KB4, KB2 and LB3 were affiliated to different genera like Syncephalastrum sp, Paecilomyces formosus, Fusarium chlamydosporum, and Coniochaeta sp., respectively. Basis on the taxonomic analysis, enzymatic activities and the hydrocarbons removal rates, single fungal culture employing the strain LB3, KB4, KBR1 and the mixed culture (LB3+KB4) were selected to be used in soil microcosms treatments. The Total petroleum hydrocarbons (TPH), fungal growth rates, BOD5/COD ratios and GC-MS analysis, were determined in all soil microcosmos treatments (SMT) and compared with those of the control (SMU). After 60 days of culture incubation, the highest rate of TPH degradation was recorded in SMT[KB4] by approximately 92±2.35% followed by SMT[KBR1] then SMT[LB3+KB4] with 86.66±1.83% and 85.14±2.21%, respectively.

Experimental Study on Bioremediation of Petroleum Contaminated Soil in Puyang Oilfield

2011 ◽  
Vol 233-235 ◽  
pp. 693-696
Author(s):  
Li Chen ◽  
Fa Wang Zhang ◽  
Shuo Ren ◽  
Sheng Zhang
Keyword(s):  
Experimental Study ◽  
Nitrogen Fixation ◽  
Contaminated Soil ◽  
Petroleum Hydrocarbon ◽  
Degradation Rate ◽  
Soil Layer ◽  
Water Oxygen ◽  
Petroleum Contaminated Soil ◽  
Ecological Remediation ◽  
Distinct Increase

According to the previous experience on remediation experiment in laboratory and field, the study on microgial ecological remediation of petroleum contaminated soil in Puyang oilfield was carried out under 3 different conditions. Here the results from remediation show that the technology of microbe cooperated with alfalfa, single microbe technology and single alfalfa technology all possess remedial effect on petroleum contaminated soil through 99-day period remediation, and the technology of microbe cooperated with alfalfa creates the best remedial mission with petroleum hydrocarbon degradation rate of 65.27%, while the rest single technologies exerts degradation rate of about 40% on petroleum hydrocarbon, Moreover, the experiment results indicate that few nutriment such as soluble salt, NO3-, Cl-,etc can infiltrate into the lower soil layer(50cm). However, the distinct increase of NH4+ in the second and third remediation area may attribute to abundance fertilizer transportation into depth soil layer due to the nitrogen fixation of alfalfa’s roots. In addition, we also find that the remediation effect can be impacted by the factors containing tempertation, water, oxygen, nutriment and mini-geo-enviroment.

scholarly journals Biodegradation of Extractable Petroleum Hydrocarbons by Consortia Bacillus cereus and Pseudomonas putida in Petroleum Contaminated-Soil

2019 ◽  
Vol 19 (2) ◽  
pp. 347 ◽  
Author(s):  
Abubakar Tuhuloula ◽  
Suprapto Suprapto ◽  
Ali Altway ◽  
Sri Rachmania Juliastuti
Keyword(s):  
Bacillus Cereus ◽  
Pseudomonas Putida ◽  
Contaminated Soil ◽  
Petroleum Hydrocarbons ◽  
Bacterial Consortium ◽  
Contaminated Site ◽  
Oil Concentration ◽  
Petroleum Contaminated Soil ◽  
And Control ◽  
Water Ratio

Contamination of soil by the activities of exploration, production, and disposal of oil waste into the environment causes serious damage to the environmental ecosystem, the target of processing by the bacteria as a model for remediation of oil contaminated site. Thus, the study was focused on determining the biodegradation percentage of extractable petroleum hydrocarbons as a function of the oil concentration. This research was conducted in a slurry bioreactor with mixed contaminated soil to water ratio of 20:80 (wt.%). A consortium of Bacillus cereus and Pseudomonas putida bacteria 10% (v/v) and 15% (v/v) with the ratio of 2:3, 1:1, and 3:2 was inserted into the slurry bioreactor and a single reactor was used as a control. The result of identification with an initial concentration of extractable petroleum hydrocarbons of 299.53 ng/µL, after 49 days of incubation for bacterial consortium 10% (v/v), the concentration was reduced to 85.31; 32.43; 59.74; and 112.22 ng/µL respectively and the biodegradation percentage was 71.5; 89.17; 80.05; and 62.54%. As for the bacterial consortium concentration of 15% (v/v) with the same ratio and control, the effluent concentration was 12.48; 7.72; 18.93 ng/µL, respectively or the biodegradation percentage was 95.83; 97.42; 93.68%.

scholarly journals Phytoremediation of hydrocarbon-contaminated soils with emphasis on the effect of petroleum hydrocarbons on the growth of plant species

2009 ◽  
Vol 89 (1) ◽  
pp. 21-29 ◽  
Author(s):  
Ravanbakhsh Shirdam ◽  
Ali Daryabeigi Zand ◽  
Gholamreza Nabi Bidhendi ◽  
Nasser Mehrdadi
Keyword(s):  
Plant Species ◽  
Contaminated Soil ◽  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Petroleum Hydrocarbon ◽  
Green Technology ◽  
Hydrocarbon Contamination ◽  
Total Petroleum Hydrocarbons ◽  
Control Treatment ◽  
Polluted Soils

To date, many developing countries such as Iran have almost completely abandoned the idea of decontaminating oil-polluted soils due to the high costs of conventional (physical/chemical) soil remediation methods. Phytoremediation is an emerging green technology that can become a promising solution to the problem of decontaminating hydrocarbon-polluted soils. Screening the capacity of native tolerant plant species to grow on aged, petroleum hydrocarbon-contaminated soils is a key factor for successful phytoremediation. This study investigated the effect of hydrocarbon pollution with an initial concentration of 40 000 ppm on growth characteristics of sorghum (Sorghum bicolor) and common flax (Linum usitatissumum). At the end of the experiment, soil samples in which plant species had grown well were analyzed for total petroleum hydrocarbons (TPHs) removal by GC-FID. Common flax was used for the first time in the history of phytoremediation of oil-contaminated soil. Both species showed promising remediation efficiency in highly contaminated soil; however, petroleum hydrocarbon contamination reduced the growth of the surveyed plants significantly. Sorghum and common flax reduced TPHs concentration by 9500 and 18500 mg kg‑1, respectively, compared with the control treatment.

scholarly journals Physicochemical characterisation of petroleum hydrocarbon contaminated land of Guru Gobind Singh refinery’s peripheral area, Punjab

2021 ◽  
Vol 22 (1&2) ◽  
pp. 213-216
Author(s):  
S.S. Sharma ◽  
A. Vashishtha
Keyword(s):  
Contaminated Soil ◽  
Petroleum Hydrocarbons ◽  
Petroleum Hydrocarbon ◽  
Living System ◽  
Chemical Parameters ◽  
Available Nitrogen ◽  
Estimated Parameters ◽  
Physico Chemical ◽  
Conductivity Water ◽  
Physicochemical Characterisation

Petroleum hydrocarbons are a critical environmental contaminant and pose a serious hazard to the living system as petroleum hydrocarbons are identified as carcinogenic and neurotoxic organic pollutants. Therefore, remedial methods are required to dispose of it. With a modern understanding of nature and microorganisms, bioremediation is the preferred method for soil pollution control. However, before the implementation of successful bioremediation technology, it is required to assess various physico-chemical parameters of contaminated soil. In the present study, various physico-chemical parameters, including pH, electrical conductivity, water holding capacity, organic carbon, organic matter, available nitrogen, carbonate, bicarbonate, potassium and sodium contents of the petroleum hydrocarbon contaminated soil were estimated. The results suggested a rise in all the estimated parameters for the petroleum hydrocarbon-contaminated soil.

scholarly journals Characterization on the rhizoremediation of petroleum contaminated soil as affected by different influencing factors

2010 ◽  
Vol 7 (3) ◽  
pp. 4665-4688 ◽  
Author(s):  
J. Tang ◽  
R. Wang ◽  
X. Niu ◽  
M. Wang ◽  
Q. Zhou
Keyword(s):  
Plant Growth ◽  
Influencing Factors ◽  
Tall Fescue ◽  
Contaminated Soil ◽  
Petroleum Hydrocarbon ◽  
Initial Period ◽  
Plant Growth Promoting Rhizobacteria ◽  
Slow Increase ◽  
Continuous Increase ◽  
Petroleum Contaminated Soil

Abstract. In this paper, pilot experiments were conducted to analyze the effect of different environmental factors on the rhizoremediation of petroleum contaminated soil. Different plant species (cotton, ryegrass, tall fescue, and alfalfa), addition of fertilizer, different concentration of TPH in soil, bioaugmentation with effective microbial agent (EMA) and PGPR, and remediation time were tested as influencing factors during bioremediation process of Total Petroleum Hydrocarbon (TPH). The result shows that the remediation process can be enhanced by different plants species with the following order: tall fescue > ryegrass > alfalfa > cotton. The degradation rate of TPH increased with increased fertilizer addition and moderate level of 20 g/m2 urea is best for both plant growth and TPH remediation. High TPH content is toxic to plant growth and inhibits the degradation of petroleum hydrocarbon with 5% TPH content showing the best degradation result in soil planted with ryegrass. Bioaugmentation with different bacteria and plant growth promoting rhizobacteria (PGPR) showed the following results for TPH degradation: cotton + EMA + PGPR > cotton + EMA > cotton + PGPR > cotton > control. Rapid degradation of TPH was found at the initial period of remediation caused by the activity of microorganisms, continuous increase was found from 30–90 d period and slow increase was found from 90 to 150 d. The result suggests that rhizoremediation can be enhanced with the proper control of different influencing factors that affect both plant growth and microbial activity in the rhizosphere environment.

Hydrocarbon treatability study of Antarctica soil with Fenton’s reagent

2021 ◽  
Author(s):  
Samuel Beal ◽  
Ashley Mossell ◽  
Jay Clausen
Keyword(s):  
Environmental Health ◽  
Contaminated Soil ◽  
Contaminated Soils ◽  
Petroleum Hydrocarbon ◽  
Total Petroleum Hydrocarbon ◽  
Fenton’S Reagent ◽  
Fenton's Reagent ◽  
Mcmurdo Station ◽  
Petroleum Contaminated Soil ◽  
Annual International Conference

The study objectives were to determine the effectiveness of Fenton’s Reagent and Modified Fenton’s Reagent in reducing Total Petroleum Hydrocarbon (TPH) concentrations in petroleum-contaminated soil from McMurdo Station, Antarctica. Comparisons of the contaminated soils were made, and a treatability study was completed and documented. This material was presented at the Association for Environmental Health and Sciences Foundation (AEHS) 30th Annual International Conference on Soil, Water, Energy, and Air (Virtual) on March 25, 2021.

Assisted phytoremediation of Cd-contaminated soil using poplar rooted cuttings

2012 ◽  
Vol 26 (3) ◽  
pp. 219-224 ◽  
Author(s):  
S. Alizadeh ◽  
G. Zahedi-Amiri ◽  
G. Savaghebi-Firoozabadi ◽  
V. Etemad ◽  
A. Shirvany ◽  
...  
Keyword(s):  
Contaminated Soil ◽  
Ethylenediaminetetraacetic Acid ◽  
Removal Rate ◽  
Cd Uptake ◽  
Rooted Cuttings ◽  
Environmental Friendly ◽  
One Year ◽  
High Removal Rate ◽  
Assisted Phytoremediation ◽  
Pot Culture

Assisted phytoremediation of Cd-contaminated soil using poplar rooted cuttings To investigate the effect of amended substrates on cadmium uptake by one-year old poplar rooted cuttings a pot culture was carried out. Pots were filled with three substrates. Four treatments of Cd supply including were organized. The results showed that higher biomass productions in substrates A and B compare to substrate C, led to an increase total Cd uptake two times more than that in substrate C, at 150 mg kg-1 concentration. Meanwhile maximum total uptake occurred in substrate B at 100 mg kg-1 concentration. Using synthetic chelators such as ethylenediaminetetraacetic acid in order to achieve high removal rate led to increased environmental impacts while they are not expected when such environmental friendly approaches are applied.

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