scholarly journals Ammonium removal efficiency of biochar-based heterotrophic nitrifying bacteria immobilization body in water solution

2020 ◽  
Vol 26 (1) ◽  
Author(s):  
Chaoxu Wang ◽  
Jing Ren ◽  
Xin Qiao ◽  
Mudassir Habib
Keyword(s):  
Water Solution ◽  
Nitrifying Bacteria ◽  
Microbial Immobilization ◽  
Adsorption Ability ◽  
Ammonium Removal ◽  
Removal Ratio ◽  
Modified Biochar ◽  
N Removal ◽  
Biological Identification ◽  
Heterotrophic Nitrifying Bacteria

In order to explore the performance of biochar-based microbial immobilization body in ammonium removal from water and potential mechanisms, a strain of heterotrophic nitrifying bacteria (HNB) was isolated from activated sludge, and the biochemical and molecular biological identification of HNB was carried out. Moreover, HNO<sub>3</sub>-, Mg<sup>2+</sup>-, NaOH<sub>-</sub>, and NaOH+Mg<sup>2+</sup>-modified rice husk-derived biochars were prepared. Then all the five kinds of biochars, including the original biochar, were used as carriers of HNB to remove NH<sub>4</sub><sup>+</sup><sub>-</sub>N from water. Results showed that HNB was classified as <i>Pseudomonas</i>, and the 72-h NH<sub>4</sub><sup>+</sup><sub>-</sub>N removal ratio of the free bacteria reached 80.24%. Compared with biochar itself, biochar-based HNB immobilization body showed a much stronger ability to remove NH<sub>4</sub><sup>+</sup><sub>-</sub>N, especially for NaOH<sub>-</sub> and NaOH+Mg<sup>2+</sup>-modified biochars. At the initial NH<sub>4</sub><sup>+</sup><sub>-</sub>N concentration of 100 mg/L and biochar addition dose of 10 g/L, NH<sub>4</sub><sup>+</sup><sub>-</sub>N removal ratio of NaOH<sub>-</sub> and NaOH+Mg<sup>2+</sup>-modified biochar-based HNB immobilization bodies reached 57.78% and 58.35% after 5 h, and reached 88.66% and 90.93% after 48 h respectively, which were obviously higher than the original, HNO<sub>3-</sub> and Mg<sup>2+</sup>-modified biochar-based HNB immobilization bodies. The phenomenon resulted from significantly higher bacteria adsorption ability of NaOH<sub>-</sub> and NaOH+Mg<sup>2+</sup>-modified biochars, which reached 773.75 and 941.17 nmol P/g biochar, respectively.

Characteristics and the Basic Kinetics of Ammonium Removal by Heterotrophic Nitrifying Bacteria at Low Temperature

2013 ◽  
Vol 807-809 ◽  
pp. 327-331
Author(s):  
Xiao Fei Huang ◽  
Wei Guang Li ◽  
Duo Ying Zhang ◽  
Wen Qin
Keyword(s):  
Low Temperature ◽  
Carbon Sources ◽  
Ammonia Nitrogen ◽  
Nitrifying Bacteria ◽  
Optimum Conditions ◽  
Songhua River ◽  
Rotating Speed ◽  
Ammonium Removal ◽  
Heterotrophic Nitrifying Bacteria ◽  
Kinetics Of

A oligotrophic heterotrophic nitrifying bacterium Y21 was isolated from Songhua River at low temperature. The influence of temperature, pH, rotating speed of shaker as well as the carbon sources on the strain Y21 was investigated in this study. The experimental results showed that for the strain Y21, the optimum conditions were found to be temperature of 15 °C, pH of 7.4~8.2, shaking speed of 140 rmp respectively, with sodium acetate as carbon source. The strain Y21 has high activity under the culture condition at low temperature. The basic dynamic equation for the degradation of ammonia-nitrogen was obtained.

Isolation, Identification and Characterization of Heterotrophic Nitrifying Bacteria from Surface Water

2013 ◽  
Vol 726-731 ◽  
pp. 406-411 ◽  
Author(s):  
Duo Ying Zhang ◽  
Wei Guang Li ◽  
Wen Qin ◽  
Xiao Fei Huang
Keyword(s):  
Drinking Water ◽  
Heterotrophic Bacteria ◽  
Removal Rate ◽  
Drinking Water Treatment ◽  
Nitrifying Bacteria ◽  
Ammonium Concentration ◽  
N Accumulation ◽  
Songhua River ◽  
Ammonium Removal ◽  
Heterotrophic Nitrifying Bacteria

For reducing ammonium concentration and guaranteeing safe drinking water, three heterotrophic bacteria were isolated from the Songhua River, which were SFA6, SFA7 and SFA11. When the initial ammonium concentration was about 130 mg/L, the ammonium removal rate of the strains SFA7, SFA6 and SFA 11 were 1.54 mg NH4+/L/h, 1.20 mg NH4+/L/h and 1.27 mg NH4+/L/h respectively at 8 °C. The 16S rDNA sequence results revealed that the strain SFA6 wasBacillus subtilis, SFA7 wasPseudomonas putida, and SFA11 showed similarity toPseudomonas nitroreducens. The biochemical characteristics of SFA6 were quite different from that of SFA7 and SFA11. After 48 h degradation, the NH4+-N (about 5 mg/L) was consumed to 0.23±0.15 mg/L, 0.37±0.20 mg/Land 0.58±0.17 mg/L by the strains SFA6, SFA7 and SFA11, with little NO3--N and NO2--N accumulation. Above all, the strains SFA6, SFA7 and SFA11 could be used in drinking water treatment at 8 °C. The strain SFA6 showed the highest ammonium removal efficiency.

scholarly journals Ammonium removal by a novel heterotrophic nitrifying and aerobic denitrifying bacterium Pseudomonas stutzeri KTB from wastewater

2015 ◽  
Vol 50 (3) ◽  
pp. 219-227 ◽  
Author(s):  
Maohong Zhou ◽  
Hairen Ye ◽  
Xiaowei Zhao
Keyword(s):  
Nitrogen Removal ◽  
Removal Rate ◽  
Pseudomonas Stutzeri ◽  
Culture Conditions ◽  
Aerobic Denitrification ◽  
Ammonium Concentration ◽  
Logarithmic Phase ◽  
Ammonium Removal ◽  
Removal Ratio ◽  
Initial Ph

The effects of culture conditions on a newly isolated Pseudomonas stutzeri KTB's ability to simultaneously perform heterotrophic nitrification and aerobic denitrification were investigated to determine its potential of application in nitrogen removal from wastewater. The results from experiments in the presence of 10 mmol/L of ammonium were as follows: succinate was the preferred carbon source, and the optimum C/N ratio, temperature, and initial pH were 10, 30 °C, and 7–8, respectively. Nitrogen removal took place not only in the logarithmic phase but also in the stationary phase. Under the optimum conditions, the nitrogen removal rate increased as the ammonium concentration elevated, until it was as high as 60 mmol/L. Meanwhile, the maximum specific growth rate decreased. The highest nitrogen removal rate of 0.977 mmol/L/h was observed at 60 mmol/L of ammonium and the maximum removal ratio of 85.6% at 40 mmol/L when the bacterial treatment for 48 h was completed. The strain was vulnerable to even higher ammonium loads. When incubated in anaerobically digested hennery wastewater containing 43.85 mmol/L of ammonium and 2.32 mmol/L of nitrate, the removal ratio and rate reached 82.4% and 0.397 mmol/L/h, respectively. The strain might be a great candidate for ammonium removal from wastewater.

scholarly journals Enhanced bacterial quorum aggregation on a zeolite capping layer for sustainable inhibition of ammonium release from contaminated sediment

2017 ◽  
Vol 76 (12) ◽  
pp. 3428-3440 ◽  
Author(s):  
Jinlan Xu ◽  
Haiyang Zhang ◽  
Rong Zhao ◽  
Fanxing Kong
Keyword(s):  
Denitrifying Bacteria ◽  
Nitrifying Bacteria ◽  
Signal Molecules ◽  
Contaminated Sediment ◽  
Sediment Remediation ◽  
Overlying Water ◽  
Regeneration Rate ◽  
Capping Layer ◽  
Ammonium Removal ◽  
Bacterial Quorum

Abstract The main objective of this study was to investigate how signal molecules enhance bacterial quorum aggregation on a zeolite capping layer for sustainable inhibition of ammonium release from contaminated sediment. Sediment remediation experiments were carried out by using nitrifying bacteria (WGX10, WGX18), denitrifying bacteria (HF3, HF7) and two kinds of signal molecules (OHHL, C8-HSL). The results showed that nitrifying bacteria and denitrifying bacteria could significantly aggregate on zeolite after adding 1.0 μM OHHL at a C/N ratio of 7. The maximum ammonium removal of five times the amount of ammonium adsorbed was achieved when 1.0 μM OHHL was added at the C/N ratio of 7 (the bio-regeneration rate was up to 88.32%), which was 1.24–2.02 times the ammonium removal amount at C/N ratios of 3, 5, 9. The concentration of total nitrogen in the overlying water was no more than 0.8 mg/L during four rounds of sediment remediation experiments. In addition, the bio-regeneration rate was up to 71.20%, which achieved sustainable inhibition of ammonium release from contaminated sediment.

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