scholarly journals A kinetic study of 4-chlorophenol biodegradation by the novel isolated Bacillus subtilis in batch shake flask

2019 ◽  
Vol 25 (1) ◽  
pp. 62-70 ◽  
Author(s):  
Sudhansu Sandhibigraha ◽  
Sagnik Chakraborty ◽  
Tarunkanti Bandyopadhyay ◽  
Biswanath Bhunia
1953 ◽  
Vol 31 (1) ◽  
pp. 28-32 ◽  
Author(s):  
A. C. Blackwood

One hundred and fourteen bacterial cultures representing most of the species in the Bacillus genus were tested for the production of extracellular barley gum cytase. Assays were made on shake-flask cultures grown on a medium containing glucose and yeast extract. Although all the organisms had some enzymatic activity, certain strains of Bacillus subtilis gave the best yields of cytase. On a medium with asparagine as the sole nitrogen source even higher yields were obtained. The crude cytase preparations were stable and after freeze-drying most of the original activity remained.


Atmosphere ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1496
Author(s):  
Eun-Hee Lee ◽  
Yunsoo Chang ◽  
Seung-Woo Lee

The coronavirus disease 2019 (COVID-19) pandemic is a general health crisis and has irreversible impacts on human societies. Globally, all people are at risk of being exposed to the novel coronavirus through transmission of airborne bioaerosols. Public health actions, such as wearing a mask, are highly recommended to reduce the transmission of infectious diseases. The appropriate use of masks is necessary for effectively preventing the transmission of airborne bioaerosols. The World Health Organization (WHO) suggests washing fabric masks or throwing away disposable masks after they are used. However, people often use masks more than once without washing or disposing them. The prolonged use of a single mask might—as a result of the user habitually touching the mask—promote the spread of pathogens from airborne bioaerosols that have accumulated on the mask. Therefore, it is necessary to evaluate how long the living components of bioaerosols can be viable on the masks. Here, we evaluated the viability of airborne Bacillus subtilis (B. subtilis) in bioaerosols filtered on woven and anti-droplet (non-woven) face masks. As a simulation of being simultaneously exposed to sand dust and bioaerosols, the viability rates of bioaerosols that had accumulated on masks were also tested against fine dust and airborne droplets containing bacteria. The bioaerosols survived on the masks immediately after the masks were used to filter the bioaerosols, and the bacteria significantly proliferated after one day of storage. Thereafter, the number of viable cells in the filtered bioaerosols gradually decreased over time, and the viability of B. subtilis in bioaerosols on the masks varied, depending on the mask material used (woven or non-woven). Despite the reduction in viability, bioaerosols containing living components were still found in both woven and anti-droplet masks even after six days of storage and it took nine days not to have found them on masks. The number of viable cells in bioaerosols on masks significantly decreased upon exposure of the masks to fine dust. The results of this study should provide useful information on how to appropriately use masks to increase their duration of effectiveness against bioaerosols.


2020 ◽  
Vol 1 (1) ◽  
pp. 31-50
Author(s):  
Yun Nian Tan ◽  
Jian Hua Zhang ◽  
Wei Ning Chen

GC-MS-based metabolomics were used to investigate metabolic changes in prawn shell waste during fermentation. Microbial strains Lactobacillus plantarum and Bacillus subtilis were co-fermented in a shake flask comprising of 5% (w/v) prawn shell waste and 20% (w/v) glucose as a carbon source. Analysis of the prawn shell waste fermentation showed a total of 376 metabolites detected in the culture supernatant, including 14 amino acids, 106 organic acids, and 90 antimicrobial molecules. Results show that the liquid fraction of the co-fermentation is promising for harvesting valuable metabolites for probiotics application.


2013 ◽  
Vol 19 (9) ◽  
pp. 2741-2743
Author(s):  
Yi-Chin Huang ◽  
Chin-Hsiang Luo ◽  
Shinhao Yang ◽  
Wei Fang ◽  
Po-Chen Hung

2011 ◽  
Vol 14 (4) ◽  
pp. 727-735 ◽  
Author(s):  
Rathinam Aravindhan ◽  
Aafreen Fathima ◽  
M. Selvamurugan ◽  
Jonnalagada Raghava Rao ◽  
Unni Nair Balachandran

Author(s):  
Patrícia Maria Rocha ◽  
Ana Carmen dos Santos Mendes ◽  
Sérgio Dantas de Oliveira Júnior ◽  
Carlos Eduardo de Araújo Padilha ◽  
Ana Laura Oliveira de Sá Leitão ◽  
...  

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