scholarly journals Autologous serum effect on corneal endothelial damage in the phacoemulsification rabbit model

2017 ◽  
Vol 8 (Suplement 4) ◽  
Keyword(s):  
Rabbit Model ◽  
Endothelial Damage ◽  
Autologous Serum ◽  
Serum Effect

scholarly journals Treatment of corneal endothelial damage in a rabbit model with a bioengineered graft using human decellularized corneal lamina and cultured human corneal endothelium

PLoS ONE ◽  
2019 ◽  
Vol 14 (11) ◽  
pp. e0225480 ◽  
Author(s):  
Francisco Arnalich-Montiel ◽  
Adrian Moratilla ◽  
Sherezade Fuentes-Julián ◽  
Veronica Aparicio ◽  
Marta Cadenas Martin ◽  
...  
Keyword(s):  
Corneal Endothelium ◽  
Rabbit Model ◽  
Endothelial Damage ◽  
Human Corneal Endothelium

scholarly journals Topical Ascorbic Acid Ameliorates Oxidative Stress-Induced Corneal Endothelial Damage via Suppression of Apoptosis and Autophagic Flux Blockage

Cells ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 943 ◽  
Author(s):  
Yi-Jen Hsueh ◽  
Yaa-Jyuhn James Meir ◽  
Lung-Kun Yeh ◽  
Tze-Kai Wang ◽  
Chieh-Cheng Huang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Endothelial Cells ◽  
Ascorbic Acid ◽  
Corneal Endothelium ◽  
Cell Damage ◽  
Rabbit Model ◽  
Endothelial Damage ◽  
Autophagic Flux ◽  
Protective Effects ◽  
Akt Phosphorylation

Compromised pumping function of the corneal endothelium, due to loss of endothelial cells, results in corneal edema and subsequent visual problems. Clinically and experimentally, oxidative stress may cause corneal endothelial decompensation after phacoemulsification. Additionally, in vitro and animal studies have demonstrated the protective effects of intraoperative infusion of ascorbic acid (AA). Here, we established a paraquat-induced cell damage model, in which paraquat induced reactive oxygen species (ROS) production and apoptosis in the B4G12 and ARPE-19 cell lines. We demonstrate that oxidative stress triggered autophagic flux blockage in corneal endothelial cells and that addition of AA ameliorated such oxidative damage. We also demonstrate the downregulation of Akt phosphorylation in response to oxidative stress. Pretreatment with ascorbic acid reduced the downregulation of Akt phosphorylation, while inhibition of the PI3K/Akt pathway attenuated the protective effects of AA. Further, we establish an in vivo rabbit model of corneal endothelial damage, in which an intracameral infusion of paraquat caused corneal opacity. Administration of AA via topical application increased its concentration in the corneal stroma and reduced oxidative stress in the corneal endothelium, thereby promoting corneal clarity. Our findings indicate a perioperative strategy of topical AA administration to prevent oxidative stress-induced damage, particularly for those with vulnerable corneal endothelia.

The Effect of Autologous Serum Eye Drops on the Conjunctivalization over Exposed Porous Polyethylene Orbital Implant (Medpor®) in the Rabbit Model

Orbit ◽  
2011 ◽  
Vol 30 (2) ◽  
pp. 83-87 ◽  
Author(s):  
Joo Hoon Kim ◽  
Ho Kyung Chung ◽  
Nam Ju Kim ◽  
Min Joung Lee ◽  
Sang In Khwarg
Keyword(s):  
Rabbit Model ◽  
Autologous Serum ◽  
Eye Drops ◽  
Porous Polyethylene ◽  
Orbital Implant ◽  
Serum Eye Drops

A Comparison of the Antithrombotic and Haemorrhagic Effects of a Low Molecular Weight Heparin (LHN-1) and Conventional Heparin

1986 ◽  
Vol 55 (03) ◽  
pp. 410-414 ◽  
Author(s):  
V Diness ◽  
J I Nielsen ◽  
P C Pedersen ◽  
K H Wolffbrandt ◽  
P B Østergaard
Keyword(s):  
Molecular Weight ◽  
Intravenous Administration ◽  
Low Molecular Weight Heparin ◽  
Thrombus Formation ◽  
Rabbit Model ◽  
Endothelial Damage ◽  
Low Molecular Weight ◽  
Dose Ratio ◽  
Experimental Thrombosis ◽  
Antithrombotic Effects

SummaryThe antithrombotic effects after intravenous administration of a low molecular weight heparin (LHN-1) and conventional heparin were compared in a rabbit model of experimental thrombosis, where thrombus formation was induced by a combination of endothelial damage and stasis. Both compounds were able to prevent thrombosis completely. However, LHN-1 was significantly less potent than conventional heparin, the ratio between doses with the same antithrombotic effect being 2.4:1 on a weight basis. Bleeding times after administration of LHN-1 and conventional heparin were determined by tail transsection in anaesthetized rats and by template bleeding in the ear of conscious pigs. Given intravenously at a dose ratio of 2.4:1 (w/w), LHN-1 affected APTT less than conventional heparin, whereas the effects on haemostasis were not significantly different. In conclusion, it was found that after intravenous administration LHN-1 prevented experimental thrombosis as effectively as conventional heparin. However, the correlation between antithrombotic and haemorrhagic effects of LHN-1 was the same as that of conventional heparin. The corresponding relation in man remains to be determined.

Ultrastructure of pulmonary microvasculature in acute endotoxemia

1978 ◽  
Vol 36 (2) ◽  
pp. 470-471
Author(s):  
R. G. Gerrity ◽  
M. Richardson
Keyword(s):  
Polymorphonuclear Leukocytes ◽  
Alveolar Epithelium ◽  
Endothelial Damage ◽  
Capillary Endothelium ◽  
Type Ii Cells ◽  
Type Ii ◽  
Interstitial Edema ◽  
Pulmonary Capillaries ◽  
Lung Ultrastructure ◽  
Fibrin Thrombi

Dogs were injected intravenously with E_. coli endotoxin (2 mg/kg), and lung samples were taken at 15 min., 1 hr. and 24 hrs. At 15 min., occlusion of pulmonary capillaries by degranulating platelets and polymorphonuclear leukocytes (PML) was evident (Fig. 1). Capillary endothelium was intact but endothelial damage in small arteries and arterioles, accompanied by intraalveolar hemorrhage, was frequent (Fig. 2). Sloughing of the surfactant layer from alveolar epithelium was evident (Fig. 1). At 1 hr., platelet-PML plugs were no longer seen in capillaries, the endothelium of which was often vacuolated (Fig. 3). Interstitial edema and destruction of alveolar epithelium were seen, and type II cells had discharged their granules into the alveoli (Fig. 4). At 24 hr. phagocytic PML's were frequent in peripheral alveoli, while centrally, alveoli and vessels were packed with fibrin thrombi and PML's (Fig. 5). In similar dogs rendered thrombocytopenic with anti-platelet serum, lung ultrastructure was similar to that of controls, although PML's were more frequently seen in capillaries in the former (Fig. 6).

The Fate of Fresh, Layered, Nonsutured and Sutured, Autogenous Cartilage in the Rabbit Model

2000 ◽  
Vol 2 (4) ◽  
pp. 256-259 ◽  
Author(s):  
Joe B. Wiseman ◽  
G. Richard Holt ◽  
Michael A. Keefe ◽  
David E. Holck ◽  
Richard L. Canaan ◽  
...  
Keyword(s):  
Rabbit Model ◽  
Autogenous Cartilage
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