Media Ethics and Infectious Disease

Ethics and Security Aspects of Infectious Disease Control ◽

10.4324/9781315580357-11 ◽

2016 ◽

pp. 161-178

Author(s):

Claire Hooker Julie Leask ◽

Catherine King

Keyword(s):

Infectious Disease ◽

Media Ethics

Download Full-text

Tissue section lifting for electron microscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100081292 ◽

1978 ◽

Vol 36 (2) ◽

pp. 86-87

Author(s):

Adrian F. van Dellen

Keyword(s):

Infectious Disease ◽

Electron Microscopy ◽

Tissue Culture ◽

Organ System ◽

Surrounding Tissue ◽

Paraffin Sections ◽

Surface Areas ◽

Specific Determination ◽

High Degree ◽

Accuracy And Repeatability

The morphologic pathologist may require information on the ultrastructure of a non-specific lesion seen under the light microscope before he can make a specific determination. Such lesions, when caused by infectious disease agents, may be sparsely distributed in any organ system. Tissue culture systems, too, may only have widely dispersed foci suitable for ultrastructural study. In these situations, when only a few, small foci in large tissue areas are useful for electron microscopy, it is advantageous to employ a methodology which rapidly selects a single tissue focus that is expected to yield beneficial ultrastructural data from amongst the surrounding tissue. This is in essence what "LIFTING" accomplishes. We have developed LIFTING to a high degree of accuracy and repeatability utilizing the Microlift (Fig 1), and have successfully applied it to tissue culture monolayers, histologic paraffin sections, and tissue blocks with large surface areas that had been initially fixed for either light or electron microscopy.

Download Full-text