scholarly journals Ultrasonographic evaluation of hG-CSF transgenic goat conceptus

2014 ◽  
Vol 21 (1) ◽  
pp. 53-59
Author(s):  
Carla Rozilene Guimarães Silva Oliveira ◽  
Carlos Henrique de Sousa Melo ◽  
Joanna Maria Gonçalves de Souza-Fabjan ◽  
Antônio Carlos de Albuquerque Teles ◽  
Ribrio Ivan Tavares Pereira Batista ◽  
...  
Keyword(s):  
Transgenic Goat

scholarly journals Adaptive Mechanisms of Goat to Heat Stress

2021 ◽  
Author(s):  
Bhabesh Mili ◽  
Tukheswar Chutia
Keyword(s):  
Heat Stress ◽  
Reproductive Performance ◽  
Harsh Environment ◽  
Hilly Terrain ◽  
Rural People ◽  
Transgenic Goat ◽  
Adaptive Mechanisms ◽  
Wide Range ◽  
Gene Level ◽  
Heat Stressor

Goat is popularly known as ‘poor man’s cow’, rears mostly by the rural people due to better adaptive capability to harsh environment. Heat stress either hot or cold; negatively influence the goat productive and reproductive performance. Both survivability and reproductive performance of goat most often depend on its ability to cope with heat stressor. Goats can rears in a wide range of environment and geography may it be hilly terrain or undulating topography due to cope with the heat stress via combination of behavioral, morphological, physiological, biochemical, metabolic, hormonal and molecular changes at the gene level. All these adaptive mechanisms and genes are important for the assessment of heat stress, adaptability and strategies for management, production of heat-tolerant transgenic goat using advance biotechnological tools for sustainable goat production in challenged environment due to climate change.

RNA Interference Against FMDV-<italic>3D</italic> Replication in Transgenic Goat

2013 ◽  
Vol 43 (5) ◽  
pp. 404-410
Author(s):  
ZhengXing LIAN ◽  
Kun YU ◽  
ShouLong DENG ◽  
Tao LU ◽  
JunCai FU ◽  
...  
Keyword(s):  
Rna Interference ◽  
Transgenic Goat

scholarly journals The copy number and integration site analysis of IGF-1 transgenic goat

2014 ◽  
Vol 34 (3) ◽  
pp. 900-910 ◽  
Author(s):  
JIAN LIN ◽  
QIANG ZHANG ◽  
LI Q. ZHU ◽  
QING H. YU ◽  
QIAN YANG
Keyword(s):  
Copy Number ◽  
Integration Site ◽  
Transgenic Goat ◽  
Site Analysis ◽  
Integration Site Analysis

30 GENERATION OF CLONED TRANSGENIC GOATS WITH CARDIAC SPECIFIC OVEREXPRESSION OF TRANSFORMING GROWTH FACTOR β1

2013 ◽  
Vol 25 (1) ◽  
pp. 162 ◽  
Author(s):  
Q. Meng ◽  
J. Hall ◽  
H. Rutigliano ◽  
X. Zhou ◽  
B. R. Sessions ◽  
...  
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor ◽  
Critical Role ◽  
Transforming Growth Factor Β1 ◽  
Transgenic Goat ◽  
First Polar Body ◽  
Fetal Fibroblasts ◽  
Transgenic Goats ◽  
Tgf Β1

Transforming growth factor β1 (TGF-β1) has a potent profibrotic function and is central to signaling cascades involved in interstitial fibrosis, which plays a critical role in the pathobiology of cardiomyopathy and contributes to diastolic and systolic dysfunction. In addition, fibrotic remodeling is responsible for generation of re-entry circuits that promote arrhythmias (Bujak and Frangogiannis 2007 Cardiovasc. Res. 74, 184–195). Due to the small size of the heart, functional electrophysiology of transgenic mice is problematic. Large transgenic animal models have the potential to offer insights into conduction heterogeneity associated with fibrosis and the role of fibrosis in cardiovascular diseases. The goal of this study was to generate transgenic goats overexpressing an active form of TGFβ-1 under control of the cardiac-specific α-myosin heavy chain promoter (α-MHC). A pcDNA3.1DV5-MHC-TGF-β1cys33ser vector was constructed by subcloning the MHC-TGF-β1 fragment from the plasmid pUC-BM20-MHC-TGF-β1 (Nakajima et al. 2000 Circ. Res. 86, 571–579) into the pcDNA3.1D V5 vector. The Neon transfection system was used to electroporate primary goat fetal fibroblasts. After G418 selection and PCR screening, transgenic cells were used for SCNT. Oocytes were collected by slicing ovaries from an abattoir and matured in vitro in an incubator with 5% CO2 in air. Cumulus cells were removed at 21 to 23 h post-maturation. Oocytes were enucleated by aspirating the first polar body and nearby cytoplasm by micromanipulation in Hepes-buffered SOF medium with 10 µg of cytochalasin B mL–1. Transgenic somatic cells were individually inserted into the perivitelline space and fused with enucleated oocytes using double electrical pulses of 1.8 kV cm–1 (40 µs each). Reconstructed embryos were activated by ionomycin (5 min) and DMAP and cycloheximide (CHX) treatments. Cloned embryos were cultured in G1 medium for 12 to 60 h in vitro and then transferred into synchronized recipient females. Pregnancy was examined by ultrasonography on day 30 post-transfer. A total of 246 cloned embryos were transferred into 14 recipients that resulted in production of 7 kids. The pregnancy rate was higher in the group cultured for 12 h compared with those cultured 36 to 60 h [44.4% (n = 9) v. 20% (n = 5)]. The kidding rates per embryo transferred of these 2 groups were 3.8% (n = 156) and 1.1% (n = 90), respectively. The PCR results confirmed that all the clones were transgenic. Phenotype characterization [e.g. gene expression, electrocardiogram (ECG), and magnetic resonance imaging (MRI)] is underway. We demonstrated successful production of transgenic goat via SCNT. To our knowledge, this is the first transgenic goat model produced for cardiovascular research. This work was supported by the Utah Science Technology and Research Initiative, Utah Multidisciplinary Arrhythmia Consortium.

scholarly journals Production of transgenic goat (Capra hircus) with human Granulocyte Colony Stimulating Factor (hG-CSF) gene in Brazil

2007 ◽  
Vol 79 (4) ◽  
pp. 585-592 ◽  
Author(s):  
Vicente J.F. Freitas ◽  
Irina A. Serova ◽  
Lyudmila E. Andreeva ◽  
Guennadi A. Dvoryanchikov ◽  
Edilson S. Lopes-Jr. ◽  
...  
Keyword(s):  
Latin America ◽  
Capra Hircus ◽  
Estrus Synchronization ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Transgenic Goat ◽  
First Report ◽  
Transgenic Goats ◽  
Stimulating Factor ◽  
Transgenic Male

In order to produce transgenic goats with hG-CSF, a total of 24 adult Saanen and 48 adult undefined breed goats were used as donors and recipients, respectively. Donors were estrus-synchronized with vaginal sponges and superovulated by a treatment with 200 mg FSH given twice daily in decreasing doses over 3 days starting 48 h before sponge removal. Ovulation was induced by injecting 100µg GnRH 36 h after sponge removal. The recipients also received an estrus synchronization treatment. Donors were mated with fertile Saanen bucks and, approximately 72 h after sponge removal, zygotes were recovered surgically by flushing oviducts. The recovered zygotes were briefly centrifuged to a reliable visualization of the pronuclei. The DNA construct containing hG-CSF gene flanked by goat and bovine alphas1-casein sequences was injected into pronuclei of 129 zygotes. The microinjected embryos (3-6 per female) were transferred to 27 recipients. Ten recipients became pregnant and 12 kids were born. One transgenic male founder was identified in the group of kids. This is the first report of a birth of a transgenic goat in Latin America.

Efficient production of transgenic goat (Capra hircus) embryos using dual markers

2008 ◽  
Vol 75 (1) ◽  
pp. 99-104
Author(s):  
Liang Wang ◽  
Tingting Liu ◽  
Tao Peng ◽  
Dajiang Zhang ◽  
Hanqing Wang ◽  
...  
Keyword(s):  
Capra Hircus ◽  
Efficient Production ◽  
Transgenic Goat

Facile purification and characterization of recombinant human antithrombin III from transgenic goat milk

2011 ◽  
Vol 86 (10) ◽  
pp. 1303-1309
Author(s):  
Yingjun Kong ◽  
Guifeng Zhang ◽  
Jian Luo ◽  
Yongdong Liu ◽  
Xiunan Li ◽  
...  
Keyword(s):  
Antithrombin Iii ◽  
Goat Milk ◽  
Purification And Characterization ◽  
Transgenic Goat

Abstract 251: Development of a Transgenic Goat Model with Cardiac-Specific Overexpression of Transforming Growth Factor-β1 to Study the Relationship Between Atrial Fibrosis and Atrial Fibrillation

2012 ◽  
Vol 111 (suppl_1) ◽  
Author(s):  
Irina A Polejaeva ◽  
Justin Hall ◽  
Qinggang Meng ◽  
Xinchang Zhou ◽  
Benjamin R Sessions ◽  
...  
Keyword(s):  
Atrial Fibrillation ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Large Animal ◽  
Atrial Pacing ◽  
Atrial Fibrosis ◽  
Transgenic Goat ◽  
Large Animal Models ◽  
Fetal Fibroblasts ◽  
Tgf Β1

Studies on patients, large animal models and transgenic mouse models have shown a strong association of atrial fibrosis with atrial fibrillation (AF). However, it is unclear whether there is a causal relationship between atrial fibrosis and AF or whether these events appear as a result of independent pathological changes in the heart. We are testing the hypothesis that goats that overexpress TGF-β1 (transforming growth factor beta1) specifically in cardiac myocytes will develop atrial fibrosis that in turn will lead to AF. Many aspects of AF-related remodeling have been studied comprehensively in goat models. However, these AF models are typically mechanically induced (eg, the rapid atrial pacing model). This unique transgenic goat model has the potential to offer insights into the role of fibrosis in AF initiation and progression without the confounding effects of mechanical AF induction. Somatic cell nuclear transfer (SCNT or cloning) was used to produce TGF-β1 transgenic pregnancies. First, pcDNA3.1DV5-MHC-TGF-β1cys33ser vector was constructed by subcloning the MHC-TGF-β1 fragment from the plasmid pUC-BM20-MHC-TGF-β1 into the pcDNA3.1D V5 vector. The NeonTM transfection system was used to electroporate primary goat fetal fibroblasts. After two weeks of G418 selection, the resulting G418 resistant colonies were screened by PCR to confirm transgene integration into goat genomic DNA. PCR positive cells were used for SCNT. Cloned embryos (n=264) were cultured for 12-60 h in vitro and then transferred into synchronized recipient females (n=15). Confirmation of pregnancy was done by ultrasonography on day 30 post-transfer. At the time of this abstract submission, 40% (6/15) of recipients were confirmed to be pregnant as determined by the presence of a heartbeat. The range for the stage of gestation is between day-60 and day-120. The first delivery date is April 28, 2012. Several reports documented no pregnancy losses after 30 days of gestation in goats. Therefore, we expect that most if not all of these pregnancies will result in delivery of live offspring. To our knowledge, this will be the first transgenic goat model generated for cardiovascular research.

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