scholarly journals Ovary maturation stages histology and follicles diameter of Melanotaenia boesemani rainbowfish ovary from district of North Ayamaru, Maybrat Regency, west Papua

2015 ◽  
Vol 32 (03) ◽  
pp. 157-164
Author(s):  
I. Hismayasari ◽  
S. Rahayu ◽  
A. Marhendra

Abstract Introduction: This research aim is to described ovary maturation stages histologically related with oocytes development in ovary of boesemani rainbowfish M. boesemani from North Ayamaru district, Maybrat Regency, West Papua. Materials and Methods: A histologycal analysis of ovari maturation stage (OMS) of the Boesemani rainbowfish Melanotaenia boesemani performed on 93 ovaries. Fresh ovaries were fixed in 10% Neutral Buffered Formalin (NBF) and embedded in paraffin. Section of about 5-6 pm thickness were cut and stained with Hematoxylin eosin (HE). Results: Histologycal analysis of rainbowfish M. boesemani ovaries based on oocyte development consist of 8 oocyte types not included atresia follicle i.e oogonia, early perinuclear oocyte, late perinuclear oocyte, cortical alveoli oocyte, early vitelogenic oocyte, mid-vitelogenic oocyte, late vitelogenic oocyte, and mature oocyte. The chorion and follicle layers begins to form at OMS II, keeps growing at OMS III, and was apparent at OMS IV. The chorion and follicle layers at OMS V ovaries were disintegrated. Conclusion: Based on ovary histology, oocyte proportion, and follicles diameter distribution can be concluded that the rainbowish M. boesemani classiied as multiple spawner.

2003 ◽  
Vol 46 (3) ◽  
pp. 433-441 ◽  
Author(s):  
Claudia Angelica da Silva Brandão ◽  
Maria de Fátima Moraes Valentim ◽  
Érica Pellegrini Caramaschi

Macro- and microscopic ovarian features of Hemiodus microlepis, H. ternetzi and H. unimaculatus were analyzed. Based on these features we proposed an ovarian maturation scale. The nine stages of the preliminary macroscopic fieldwork scale were reduced to five after microscopic analysis of ovaries. The microscopic analysis indicated a group-synchronous oocyte development common to the three species that were characterized as iteroparous synchronous spawners with a total spawning type. The remarkable thickness of the zona radiata layer and the large size of vitellogenic oocytes of Hemiodus ternetzi distinguished this species from the others.


2020 ◽  
Vol 18 (3) ◽  
Author(s):  
Santiago Julián Bianchi ◽  
Mariano González-Castro

ABSTRACT The reproductive biology and life cycle of Parona leatherjacket, Parona signata, present in Mar del Plata (38º 00’S 57º 33’W) coast, was studied. Samples were obtained monthly since January 2018 to February 2019 from the artisanal fishermen and the commercial fleet of Mar del Plata. A histological analysis was carried out and the main biologic-reproductive parameters were estimated: fecundity, oocyte frequency distribution and gonadosomatic index (GSI). Both the macroscopic and microscopic analyses showed reproductive activity in March and November. Mature females were recorded, which showed hydrated oocytes, as was evidenced by the histological procedures. Both, the histological and the oocyte diameter distribution analyses showed the presence of all oocyte maturation stages in ovaries in active-spawning subphase, indicating that P. signata is a multiple spawner with indeterminate annual fecundity. Batch fecundity ranged between 36,426 and 126,035 hydrated oocytes/ female. Relative fecundity ranged between 42 and 150 oocytes/ g female ovary free.


2017 ◽  
Vol 39 (4) ◽  
pp. 374-384
Author(s):  
Cesar Pedro Hartmann Filho ◽  
André Luís Duarte Goneli ◽  
Tathiana Elisa Masetto ◽  
Elton Aparecido Siqueira Martins ◽  
Guilherme Cardoso Oba

Abstract: This study evaluated the physiological potential of soybean seeds harvested during two seasons, on different maturation stages and subjected to different drying temperatures. The seeds were harvested at the maturations stages R7, R7 + 2, R7 + 3, R7 + 5, R7 + 6, R7 + 7, R7 + 10 and R7 + 12 days (55, 50, 45, 40, 35, 30, 25, and 20% of moisture content). For each maturation stage, seeds were divided into three samples: one sample was used to directly evaluate the physiological potential, and the others were dried at 40 °C and 50 °C, until reaching the moisture content of 11.5%. The physiological potential was evaluated through germination test, first germination count of germination, accelerated aging, modified cold, electrical conductivity and seedling emergence. The maximum physiological potential of seeds is achieved at the moisture content of 55%, the point that the dry matter is maximum. The seeds became tolerant to artificial drying approximately at the stage R7 + 7 days (30% of moisture content). Germination and vigor of the soybean seeds reduce as the drying temperature is increased from 40 °C to 50 °C, and this effect is enhanced when the seeds show moisture contents above 30%.


2021 ◽  
Author(s):  
Leen Beller ◽  
Ward Deboutte ◽  
Gwen Falony ◽  
Sara Vieira Silva ◽  
Raul Tito ◽  
...  

Background: Disturbances in the primary colonization of the infant gut can result in life-long consequences and have been associated with a range of host conditions. Although early life factors have been shown to affect the infant gut microbiota development, our current understanding of the human gut colonization in early life remains limited. To gain more insights in the unique dynamics of this rapidly evolving ecosystem, we investigated the microbiota over the first year of life in eight densely sampled infants (total number of samples, n=303). To evaluate gut microbiota maturation transition towards an adult configuration, we compared the microbiome composition of the infants to the Flemish Gut Flora Project population (n=1,106). Results: We observed the infant gut microbiota to mature through three distinct, conserved stages of ecosystem development. Across these successional gut microbiota maturation stages, genus predominance was observed to shift from Escherichia over Bifidobacterium to Bacteroides. Both disease and antibiotic treatment were observed to be associated occasionally with gut microbiota maturation stage regression, a transient setback in microbiota maturation dynamics. Although the studied microbiota trajectories evolved to more adult-like constellations, microbiome community typing against the background of the Flemish Gut Flora Project (FGFP) cohort clustered all infant samples within the (in adults) potentially dysbiotic Bact2 enterotype. Conclusion: We confirmed similarities between infant gut microbial colonization and adult dysbiosis. A profound knowledge about the primary gut colonization process in infants might provide crucial insights into how the secondary colonization of a dysbiotic adult gut can be redirected.


2012 ◽  
Vol 4 (1) ◽  
Author(s):  
Retno Andamari ◽  
Jhon Haryanto Hutapea ◽  
Budi Iskandar Prisantoso

<p>Tuna is one of the important fishery commodities in Indonesia, including tuna caught by longliners in Indian Ocean. The most popular of tuna is yellowfin tuna (Thunnus albacares). Currently, the production of tuna based on the capture fisheries, therefore, in order to preserve its sustainability, the understanding of the biological and reproduction aspect is required. Research on the biological reproduction of this species was conducted at Gondol Research Institute for Mariculture. Samples of female gonad were collected from Indian Ocean tuna longliners during April to July 2009 from the processing companies within Benoa Port. Result of the histological analysis on 128 female gonads showed that majority of gonads were at the early development level and the only 16 gonads have reached at the mature level. The analysis of the oocyte development showed the asynchronous, indicating that yellowfin tuna is a multiple spawner.</p><p>Keywords: yellowfin tuna, gonad, asynchronous</p>


2015 ◽  
Vol 13 (1) ◽  
pp. 221-228 ◽  
Author(s):  
Sofía López ◽  
Ezequiel Mabragaña ◽  
Juan Martín Díaz de Astarloa ◽  
Mariano González-Castro

The aim of the present study provides the first results on the reproductive biology of the anchovy, Anchoa marinii, in a nearby-coastal area of Mar Chiquita coastal lagoon, 37º32'00"S 57º19'00"W, Argentina. To perform this, we employed mainly female gonads, at both microscopic and macroscopic levels. The oocyte diameter distribution and the microscopy analysis of oocytes, showed different batches of growing oocytes, suggesting that the anchovy is a multiple spawner, with constant oocytes recruitment all over the reproductive season. The batch fecundity ranged between 749 and 3,207 oocytes for females, and the relative fecundity ranged from 127 to 422 yolked oocytes per female gram (ovary free). The fecundity estimation has been made with yolked oocytes. No correlation between the fecundity estimation and female size and total weight (without ovary) has been found. The estimated L50 values were 74.13 and 73.76 mm TL for females and males, respectively. Shoals of females in advanced maturity phase were recorded, between December to April in the study area. During this period a significant increase of species abundance was correlated with water temperature increase (above 16°C). This fact, together with the detection of post-ovulatory follicles, indicated the existence of reproductive events in the nearby-coastal area of Mar Chiquita coastal lagoon.


2018 ◽  
Vol 31 (4) ◽  
pp. 963-971 ◽  
Author(s):  
WELSON LIMA SIMÕES ◽  
MARCELO CALGARO ◽  
MIGUEL JULIO MACHADO GUIMARÃES ◽  
ANDERSON RAMOS DE OLIVEIRA ◽  
MÍRIAN PAULA MEDEIROS ANDRÉ PINHEIRO

ABSTRACT Sugarcane is one of the most affected crops by water scarcity. The efficient use of the irrigation water is an alternative to minimize this problem. The objective of this work was to evaluate biometric parameters, yield, and technological quality of sugarcane plants subjected to different controlled water deficit regimes in the sub-middle São Francisco Valley, Brazil. The experiment was conducted in a randomized block design, in two crop cycles, with three replications, with ten treatments consisted of three controlled water deficits (15%, 30%, and 45% of the crop evapotranspiration - ETc), applied at three development stages of the plant - sprouting and tillering (Stage I), grand growth (Stage II), and maturation (Stage III) - and a control with 100% of the ETc throughout the entire crop cycle. The controlled water deficit did not affect the technological quality of the sugarcane in any development stage. The sugarcane yield was higher when using a controlled water deficit of 30% of ETc in the sprouting and tillering stages of the plants. The water deficit of 15% of ETc is recommended for the grand growth, or maturation stages of the sugarcane plants for a greater water use efficiency of the production system.


2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Ludy Marileidy Jimenez-Valdivia ◽  
Violeta Malpartida-Carrillo ◽  
Yalil Augusto Rodríguez-Cárdenas ◽  
Heraldo Luis Dias-Da Silveira ◽  
Luis Ernesto Arriola-Guillén

Abstract Background The aim of this study was to evaluate the midpalatal suture maturation stages in adolescents and young adults using cone-beam computed tomography (CBCT). Methods The sample comprised 200 CBCT scans of individuals aged 10 to 25 years old (95 males and 105 females) divided into three groups, adolescents (n = 48), post-adolescents (n = 52), and young adults (n = 100). The Planmeca ProMax 3D software was used for the midpalatal suture maturation stage evaluation according to Angieleri’s method, using cross-sectional axial slice. Two previously calibrated examiners analyzed the images and classified according to five different maturation stages. A, B, and C stages were considered with open midpalatal suture, and D and E were considered without open midpalatal suture. Association tests were performed using chi-square test also, and a binary logistic regression was evaluated (P < 0.05). Results The possibility to find open midpalatal suture in individuals of 10 to 15 years old was 70.8%, in subject aged 16 to 20 and 21 to 25 years old was 21.2% and 17%, respectively. Furthermore, this possibility in individuals older than 16 years was greater in males than in females. Conclusions The possibility to find open midpalatal suture in post-adolescents and young adults is greater than the orthodontists considered years ago. Furthermore, men are more likely to find midpalatal suture opening. These implications might be considered by the orthodontists when maxillary expansion is required. Besides, the ossification of the middle palatal suture is very variable, and therefore, the use of CBCT might be recommended to clarify this possibility.


2015 ◽  
Vol 75 (2) ◽  
pp. 324-330
Author(s):  
MR. Oliveira ◽  
NB. Silva ◽  
ME. Yamamoto ◽  
S. Chellappa

The gonad development and reproductive aspects of Hemiramphus brasiliensis from the coastal waters of Rio Grande do Norte, Brazil were verified. This paper presents data on the length-weight relationship, sex ratio, length at first sexual maturity, gonad development, reproductive period and fecundity of H. brasiliensis. Females of this species predominated in the sampled population and were larger in size than the males. The length at the first sexual maturation of males was 20.8 cm and that of females was 21.5 cm. The macroscopic characteristics of the gonads indicated four maturation stages. Histological studies of gonads of H. brasiliensis showed six phases of oocyte development and four phases of spermatocyte development. The batch fecundity of this species is 1153 (±258.22) oocytes for 50 g body weight of female. The microscopic characteristics of gonad development indicate that H. brasiliensis is a multiple spawner, with active reproductive period during the months of January to June and October. The reproductive period of this species is independent of the rainy period of the region.


2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Mônica Rocha de Oliveira ◽  
Sathyabama Chellappa

The reproductive aspects ofHemiramphus brasiliensiswere analyzed with a view to verify the temporal dynamics of reproduction. This paper presents data on sex ratio, length at first sexual maturity, macroscopic and histological aspects of gonad development, gonadosomatic index (GSI), reproductive period, and fecundity ofH. brasiliensis. The fishes were captured from the coastal waters of Rio Grande do Norte, northeastern Brazil. Females of this species predominated in the sampled population and were larger in size than the males. The length at the first sexual maturation of males was 20.8 cm and that of females was 21.5 cm. The macroscopic characteristics of the gonads indicated four maturation stages. Histological studies of gonads ofH. brasiliensisshowed six phases of oocyte development and four phases of spermatocyte development. The batch fecundity of this species was 1153 (±258.22) mature oocytes for 50 g body weight of female. The microscopic characteristics of gonad development indicate thatH. brasiliensisis a multiple spawner, presenting a prolonged reproductive period during the whole year, with a peak in the month of April, and is considered as an opportunistic strategist.


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