scholarly journals Direct estimation of functional PSII reaction center concentration and PSII electron flux on a volume basis: a new approach to the analysis of Fast Repetition Rate fluorometry (FRRf) data

2012 ◽  
Vol 10 (3) ◽  
pp. 142-154 ◽  
Author(s):  
Kevin Oxborough ◽  
C. Mark Moore ◽  
David J. Suggett ◽  
Tracy Lawson ◽  
Hoi Ga Chan ◽  
...  
Keyword(s):  
Reaction Center ◽  
Repetition Rate ◽  
Electron Flux ◽  
New Approach ◽  
Psii Reaction Center ◽  
Direct Estimation ◽  
Fast Repetition Rate Fluorometry

Use of Fast Repetition Rate Fluorometry on Detection and Assessment of PAH Toxicity on Microalgae

2009 ◽  
Vol 209 (1-4) ◽  
pp. 345-356 ◽  
Author(s):  
Patricia Pérez ◽  
Emilio Fernández ◽  
Ricardo Beiras
Keyword(s):  
Repetition Rate ◽  
Fast Repetition Rate Fluorometry ◽  
Pah Toxicity

A new approach to predict and estimate enhancements of “killer” electron flux at geosynchronous orbit

2016 ◽  
Vol 126 ◽  
pp. 47-51 ◽  
Author(s):  
Alexander Potapov ◽  
Larisa Ryzhakova ◽  
Battuulai Tsegmed
Keyword(s):  
Electron Flux ◽  
Geosynchronous Orbit ◽  
New Approach

scholarly journals Development of photosynthetic carbon fixation model using multi-excitation wavelength fast repetition rate fluorometry in Lake Biwa

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0238013
Author(s):  
Takehiro Kazama ◽  
Kazuhide Hayakawa ◽  
Victor S. Kuwahara ◽  
Koichi Shimotori ◽  
Akio Imai ◽  
...  
Keyword(s):  
Repetition Rate ◽  
Carbon Fixation ◽  
Lake Biwa ◽  
Freshwater Ecosystems ◽  
Cyanobacterial Blooms ◽  
Excitation Wavelength ◽  
Freshwater Ecosystem ◽  
Fixation Rate ◽  
Excitation Light ◽  
Fast Repetition Rate Fluorometry

Direct measurements of gross primary productivity (GPP) in the water column are essential, but can be spatially and temporally restrictive. Fast repetition rate fluorometry (FRRf) is a bio-optical technique based on chlorophyll a (Chl-a) fluorescence that can estimate the electron transport rate (ETRPSII) at photosystem II (PSII) of phytoplankton in real time. However, the derivation of phytoplankton GPP in carbon units from ETRPSII remains challenging because the electron requirement for carbon fixation (Фe,C), which is mechanistically 4 mol e− mol C−1 or above, can vary depending on multiple factors. In addition, FRRf studies are limited in freshwater lakes where phosphorus limitation and cyanobacterial blooms are common. The goal of the present study is to construct a robust Фe,C model for freshwater ecosystems using simultaneous measurements of ETRPSII by FRRf with multi-excitation wavelengths coupled with a traditional carbon fixation rate by the 13C method. The study was conducted in oligotrophic and mesotrophic parts of Lake Biwa from July 2018 to May 2019. The combination of excitation light at 444, 512 and 633 nm correctly estimated ETRPSII of cyanobacteria. The apparent range of Фe,C in the phytoplankton community was 1.1–31.0 mol e− mol C−1 during the study period. A generalised linear model showed that the best fit including 12 physicochemical and biological factors explained 67% of the variance in Фe,C. Among all factors, water temperature was the most significant, while photosynthetically active radiation intensity was not. This study quantifies the in situ FRRf method in a freshwater ecosystem, discusses core issues in the methodology to calculate Фe,C, and assesses the applicability of the method for lake GPP prediction.

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