scholarly journals A shipboard natural community continuous culture system for ecologically relevant low-level nutrient enrichment experiments

2003 ◽  
Vol 1 (1) ◽  
pp. 82-91 ◽  
Author(s):  
David A. Hutchins ◽  
Frances Pustizzi ◽  
Clinton E. Hare ◽  
Giacomo R. DiTullio
Keyword(s):  
Continuous Culture ◽  
Nutrient Enrichment ◽  
Culture System ◽  
Natural Community ◽  
Low Level ◽  
Continuous Culture System ◽  
Enrichment Experiments

Kinetics of biodegradation of Di-n-butyl phthalate in continuous culture system

Chemosphere ◽  
1998 ◽  
Vol 37 (2) ◽  
pp. 257-264 ◽  
Author(s):  
Jianlong Wang ◽  
Ping Liu ◽  
Hanchang Shi ◽  
Yi Qian
Keyword(s):  
Continuous Culture ◽  
Culture System ◽  
Continuous Culture System ◽  
Butyl Phthalate ◽  
Kinetics Of

Three-stage continuous culture system with a self-generated anaerobia to study the regionalized metabolism of the human gut microbiota

2014 ◽  
Vol 96 ◽  
pp. 111-118 ◽  
Author(s):  
David Feria-Gervasio ◽  
William Tottey ◽  
Nadia Gaci ◽  
Monique Alric ◽  
Jean-Michel Cardot ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Continuous Culture ◽  
Culture System ◽  
Human Gut ◽  
Human Gut Microbiota ◽  
Continuous Culture System

scholarly journals Design and development of a continuous culture system for studying rumen fermentation

1989 ◽  
Vol 61 (5) ◽  
pp. 463-473
Author(s):  
Harri Miettinen ◽  
Jouko Setälä
Keyword(s):  
Continuous Culture ◽  
Culture System ◽  
Artificial Saliva ◽  
Present Report ◽  
Ammonia Concentration ◽  
Rumen Fermentation ◽  
Liquid Volume ◽  
Desktop Computer ◽  
Continuous Culture System

The present report describes an in vitro continuous culture system to simulate rumen fermentation. The complete assembly consists of six culture vessels (liquid volume 700 ml) fed twice daily with finely ground feed. The artificial saliva enters the vessel continuously, and the effluent leaves it continuously through the overflow port. The intermittent stirring of the fermentor content and the pH regulation are automatically controlled by a desktop computer. Two replicate experiments with ten fermentors given a diet of silage (50 %) and barley (50 %) were made in order to evaluate the system. The results indicate that the system reaches steady-state conditions within three to five days, ammonia concentration being an exeption. It takes for the ammonia concentration approximately 11—14 days to stabilize. The plateau values for the total volatile fatty acid (VFA) concentrations, the molar proportions of individual VFAs, and the ammonia concentrations were found to be within the accepted range in the rumen of animals given similar diets or in other artificial rumen systems. There was a tenfold decrease in the numbers of protozoa in the fermentors during the first four days of incubation. However, the average plateau value for the protozoa numbers (2.5 x 104/ml) is in the same range as in the dual flow continuous culture systems. The efficiency of the microbial N production was higher than that usually observed in vivo or in vitro (45 vs. 30 g/kg organic matter digested). The results indicate that this continuous culture system provides a reasonable estimate of rumen fermentation.

293 Nutritional evaluation of forage ephedra (Ephedra nevadensis) as an alternative forage using a dual-flow continuous culture system

2017 ◽  
Vol 95 (suppl_4) ◽  
pp. 145-145
Author(s):  
C. B. Sampaio ◽  
E. Marostegan de Paula ◽  
L. Galoro da Silva ◽  
V. Brandao ◽  
X. Dai ◽  
...  
Keyword(s):  
Continuous Culture ◽  
Culture System ◽  
Nutritional Evaluation ◽  
Continuous Culture System ◽  
Ephedra Nevadensis

Biodegradation of Natural Rubber by Actinomycete No. 4 in a Continuous Culture System

2002 ◽  
Vol 5 (3) ◽  
pp. 329-331 ◽  
Author(s):  
Che Husna Azhari ◽  
Aulia Saad ◽  
Wan Mohtar Wan Yusoff ◽  
A. Ikram .
Keyword(s):  
Natural Rubber ◽  
Continuous Culture ◽  
Culture System ◽  
Continuous Culture System

scholarly journals Colonization of Mucin by Human Intestinal Bacteria and Establishment of Biofilm Communities in a Two-Stage Continuous Culture System

2005 ◽  
Vol 71 (11) ◽  
pp. 7483-7492 ◽  
Author(s):  
Sandra Macfarlane ◽  
Emma J. Woodmansey ◽  
George T. Macfarlane
Keyword(s):  
Continuous Culture ◽  
Culture System ◽  
Laser Scanning ◽  
Intestinal Bacteria ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Culture Methods ◽  
Two Stage ◽  
Bacterial Populations ◽  
Continuous Culture System

ABSTRACT The human large intestine is covered with a protective mucus coating, which is heavily colonized by complex bacterial populations that are distinct from those in the gut lumen. Little is known of the composition and metabolic activities of these biofilms, although they are likely to play an important role in mucus breakdown. The aims of this study were to determine how intestinal bacteria colonize mucus and to study physiologic and enzymatic factors involved in the destruction of this glycoprotein. Colonization of mucin gels by fecal bacteria was studied in vitro, using a two-stage continuous culture system, simulating conditions of nutrient availability and limitation characteristic of the proximal (vessel 1) and distal (vessel 2) colon. The establishment of bacterial communities in mucin gels was investigated by selective culture methods, scanning electron microscopy, and confocal laser scanning microscopy, in association with fluorescently labeled 16S rRNA oligonucleotide probes. Gel samples were also taken for analysis of mucin-degrading enzymes and measurements of residual mucin sugars. Mucin gels were rapidly colonized by heterogeneous bacterial populations, especially members of the Bacteroides fragilis group, enterobacteria, and clostridia. Intestinal bacterial populations growing on mucin surfaces were shown to be phylogenetically and metabolically distinct from their planktonic counterparts.

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