scholarly journals Assessment of Wound Healing Activity of Green Synthesized Titanium Oxide Nanoparticles using Strychnos spinosa and Blighia sapida

2020 ◽  
Vol 24 (2) ◽  
pp. 197-206
Author(s):  
H. Hassan ◽  
K.I. Omoniyi ◽  
F.G. Okibe ◽  
A.A. Nuhu ◽  
E.G. Echioba
Keyword(s):  
Wound Healing ◽  
Titanium Oxide ◽  
Protein Profiling ◽  
Oxide Nanoparticles ◽  
Titanium Oxide Nanoparticles ◽  
Tio2 Nps ◽  
Vis Spectroscopy ◽  
Strychnos Spinosa ◽  
Wound Healing Activity ◽  
Blighia Sapida

The biosynthesis of nanoparticles has been proposed as a cost effective and environmentally friendly alternative to chemical and physical methods. The present work investigates the synthesis of titanium oxide nanoparticles (TiO2 NPs) by green approach using Strychnos spinosa and Blighia sapida leave extracts. The detailed characterization of the TiO2 NPs was carried out using UV-Visible Spectroscopy, Scanning Electron Microscopy (SEM), X- ray Diffraction (XRD), and Fourier-Transform Infrared (FTIR) Spectroscopy. The green synthesized TiO2 NPs excitation was confirmed using UV–Vis spectrophotometer at 270 and 290 nm for Strychnos spinosa and Blighia sapida respectively. SEM revealed that the synthesized TiO2 NPs were spherical and crystalline in nature. The overall sizes are 40 and 50 nm for Strychnos spinosa and Blighia sapida respectively. FTIR spectroscopic analysisshowed the presence of flavonoids, polyphenols and amide groups likely to be responsible for the green synthesis of titanium oxide nanoparticles using S. Spinosa and B .sapida aqueous leaf extracts. The XRD pattern showed the characteristic Bragg peaks of (111), (200), (220) and (311) facets of the anatase titanium oxide nanoparticles and confirmed that these nanoparticles were crystalline and spherical in nature. Furthermore, the green synthesized TiO2 NPs wound healing activity was examined in the excision wound model by measuring wound closure, histopathology and protein profiling. This revealed significant wound healing activity in Albino rats. In the present study, topical application ofnanoformulated extracts of B. sapidaa nd S. spinosa significantly accelerated wound healing with 20% nanoformulated ointment having the highest percentage wound contraction ability comparable with gentamicin (a commercially sold antimicrobial agent used in dressing wounds). In conclusion, this work proved the capability of using TiO2 NPs to deliver a novel therapeutic route for wound treatment in clinical practice. Keywords: Wound healing activity; SEM; TiO2; XRD; FTIR; UV–Vis spectroscopy.

scholarly journals Evaluation of Antibacterial Potential of Biosynthesized Plant Leave Extract Mediated Titanium Oxide Nanoparticles using Hypheae Thiebeace and Anannos Seneglensis

2019 ◽  
Vol 23 (10) ◽  
pp. 1795-1804
Author(s):  
H. Hassan ◽  
K.I. Omoniyi ◽  
F.G. Okibe ◽  
A.A. Nuhu ◽  
E.G. Echioba
Keyword(s):  
Antimicrobial Activity ◽  
Green Synthesis ◽  
Titanium Oxide ◽  
Microbial Inactivation ◽  
Oxide Nanoparticles ◽  
Leaf Extracts ◽  
Titanium Oxide Nanoparticles ◽  
Tio2 Nps ◽  
Complete Inactivation ◽  
Vis Spectroscopy

The need for new antimicrobial agent has drawn attention on developing new and emerging materials based on nanoparticles with antimicrobial activity. The aim of this research was to evaluate the antibacterial activity of nanoparticles of titanium dioxide. A green synthesis of TiO2 nanoparticles was done using a plant extract of H. thelbiecea and Ananos seneglensisa The presence of various photochemical like flavonoids, steroids, polyphenols, and terpenoids was investigated by following standard biochemical methods. The titanium oxide nanoparticles (TiO2 NPs) synthesized was confirmed by their change of colour to brown and reddish brown due to the phenomenon of surface Plasmon resonance. The characterization studied was done by UV-vis spectroscopy, scanning electron microscopy (SEM), X-Ray diffraction (XRD) and Fourier Transmission infrared spectroscopy (FTIR). The green synthesized TiO2 NPs excitation was confirmed using UV–Vis spectrophotometer at 270 and 290 nm. SEM revealed that the synthesized TiO2 NPs are spherical and crystalline in nature. The overall sizes are 40 and 50 nm for H. thelbiecea and Ananos respectively. FTIR spectroscopy analysis showed the presence of flavonoid, polyphenols and amide groups likely to be responsible for the green synthesis of titanium oxide nanoparticles using H. thelbiecea and Ananos seneglensis aqueous leaf extracts .The XRD pattern showed the characteristic Bragg peaks of (111), (200), (220) and (311) facets of the anatase titanium oxide nanoparticles and confirmed that these nanoparticles are crystalline and spherical in nature. The two plants used to synthesized titanium oxide nanoparticle (H. thelbiecea and ananos seneglensisa) showed good antimicrobial activity against clinically important pathogens.. The antimicrobial study of TiO2 NPs shows that 20 μg/ml TiO2 NPs is effective for complete inactivation of Gram positive, Gram negative as well as fungal cultures. This effective microbial inactivation is mainly attributed to its ability to cause damage to the cell membrane.Keywords: Titanium oxide, phytochemicals, antimicrobial activity, H. thelbiecea, Ananos seneglensisa

scholarly journals Novel Synthesis of Titanium Oxide Nanoparticles: Biological Activity and Acute Toxicity Study

2021 ◽  
Vol 2021 ◽  
pp. 1-14
Author(s):  
Moaaz T. Hamed ◽  
Basant A. Bakr ◽  
Yahya H. Shahin ◽  
Bassma H. Elwakil ◽  
Marwa M. Abu-Serie ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Titanium Oxide ◽  
Human Cancer ◽  
Toxicity Study ◽  
Oxide Nanoparticles ◽  
Human Cancer Cell ◽  
Titanium Oxide Nanoparticles ◽  
Tio2 Nps ◽  
Human Cancer Cell Lines ◽  
Acute Toxicity Study

Titanium oxide nanoparticles (TiO2 NPs) have been attracting numerous research studies due to their activity; however, there is a growing concern about the corresponding toxicity. Here in the present study, titanium oxide nanoparticles were newly synthesized using propolis extract followed by antimicrobial activity, cytotoxicity assay using human cancer cell lines, and acute toxicity study. The physicochemical characterization of the newly synthesized TiO2 NPs had average size = 57.5 nm, PdI = 0.308, and zeta potential = −32.4 mV. Antimicrobial activity assessment proved the superior activity against Gram-positive compared to Gram-negative bacteria and yeast (lowest MIC values 8, 32, and 32, respectively). The newly synthesized TiO2 NPs showed a potent activity against the following human cancer cell lines: liver (HepG-2) (IC50 8.5 µg/mL), colon (Caco-2), and breast (MDA-MB 231) (IC50 11.0 and 18.7 µg/mL). In vivo acute toxicity study was conducted using low (10 mg/kg) and high (1000 mg/kg) doses of the synthesized TiO2 NPs in albino male rats. Biochemistry and histopathology of the liver, kidney, and brain proved the safety of the synthesized TiO2 NPs at low dose while at high dose, there was TiO2 NPs deposit in different vital organs except the cerebral tissue.

scholarly journals Magnetic Field – Assisted Laser Ablation of Titanium Dioxide Nanoparticles in Water for Anti-Bacterial Applications

2021 ◽  
Author(s):  
Hasan H. Bahjat ◽  
R.A. Ismail ◽  
Ghassan M. Sulaiman ◽  
Majid S. Jabir
Keyword(s):  
Magnetic Field ◽  
Electron Microscopy ◽  
Laser Ablation ◽  
Titanium Oxide ◽  
Oxide Nanoparticles ◽  
Titanium Oxide Nanoparticles ◽  
X Ray ◽  
Vis Spectroscopy ◽  
The Magnetic Field ◽  
Scanning Electron

Abstract Titanium oxide nanoparticles (TiO2) were produced by pulsed Nd:YAG laser ablation in water under the effect of an external magnetic field. Various techniques such as X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), energy Dispersive x-ray (EDX), transmission electron microscopy (TEM), UV-Vis spectroscopy, and Raman spectroscopy were used to characterize the TiO2 nanoparticles. The XRD analysis of titanium oxide nanoparticles revealed that the synthesized nanoparticles were polycrystalline with mixed of tetragonal anatase and rutile TiO2. Scanning electron microscope shows the formation of spherical nanoparticles and the particles agglomeration decreases and the particle size from increases from 25nm to 35nm when the magnetic field applied. The optical energy gap of TiO2 nanoparticles decreased from 4.6eV to 3.4eV after using the magnetic field during the ablation. Raman studies show the existence of five vibration modes belong to TiO2. The antibacterial effect assay revealed a largest inhibition zone in S. aureus and E. coli, with a more potent effect for TiO2 NPs prepared by magnetic field when compared with that prepared without presence of magnetic field.

Titanium Oxide Nanoparticles Improve the Chemotherapeutic Action of Erlotinib in Liver Cancer Cells

2020 ◽  
Vol 16 (4) ◽  
pp. 337-343
Author(s):  
Shaimaa E. Abdel-Ghany ◽  
Eman El-Sayed ◽  
Nour Ashraf ◽  
Nada Mokhtar ◽  
Amany Alqosaibi ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Titanium Oxide ◽  
Phase Distribution ◽  
Oxide Nanoparticles ◽  
Titanium Oxide Nanoparticles ◽  
Liver Cancer Cells ◽  
M Phase ◽  
Apoptosis Detection ◽  
Novel Method

Background: Hepatocellular carcinoma is the second leading cause of cancer-related deaths among other types of cancer due to lack of effective treatments and late diagnosis. Nanocarriers represent a novel method to deliver chemotherapeutic drugs, enhancing their bioavailability and stability. Methods: In the present study, we loaded gold nanoparticles (AuNPs) and titanium oxide nanoparticles (TiO2NPs) with ERL to investigate the efficiency of the formed composite in inducing apoptosis in HepG2 liver cancer cells. Cytotoxicity was assessed using MTT assay and cell phase distribution was assessed by flow cytometry along with apoptosis detection. Results: Data obtained indicated the efficiency of the formed composite to significantly induce cell death and arrest cell cycle and G2/M phase. IRF4 was downregulated after treatment with loaded ERL. Conclusion: Our data showed that loading ERL on TiO2NPs was more efficient than AuNPs. However, both nanocarriers were efficient compared with control.

Sign in / Sign up

Export Citation Format

Share Document