Evaluation of Total Bilirubin Concentration Measurement in CO-oximeter

2008 ◽  
Vol 78 (7) ◽  
pp. 427-432
Author(s):  
Takashi Makaya ◽  
Daisuke Yamashita ◽  
Gaku Sugihara ◽  
Hideki Kojima ◽  
Shigeru Sano ◽  
...  
Keyword(s):  
Total Bilirubin ◽  
Concentration Measurement ◽  
Bilirubin Concentration ◽  
Total Bilirubin Concentration

scholarly journals Single-Dose Pharmacokinetics of Amprenavir, a Human Immunodeficiency Virus Type 1 Protease Inhibitor, in Subjects with Normal or Impaired Hepatic Function

2000 ◽  
Vol 44 (4) ◽  
pp. 821-826 ◽  
Author(s):  
Laurence Veronese ◽  
Jacques Rautaureau ◽  
Brian M. Sadler ◽  
Catherine Gillotin ◽  
Jean-Pierre Petite ◽  
...  
Keyword(s):  
Liver Disease ◽  
Healthy Volunteers ◽  
Total Bilirubin ◽  
Laboratory Data ◽  
Hepatic Insufficiency ◽  
Pharmacokinetic Parameters ◽  
Control Group ◽  
Bilirubin Concentration ◽  
Total Bilirubin Concentration ◽  
Severe Cirrhosis

ABSTRACT Amprenavir (141W94) is extensively metabolized by P450 cytochromes, specifically, CYP3A4. Because hepatic insufficiency reduces P450-mediated metabolism, the concentrations in plasma of drugs metabolized through this pathway are often increased in subjects with liver disease. Following administration of a single, oral dose of 600 mg of amprenavir, pharmacokinetic parameters were determined for 10 subjects with severe cirrhosis, 10 subjects with moderate cirrhosis, and 10 healthy volunteers. Model-independent methods for determining the area under the plasma concentration-time curve (AUC) from time zero to infinity (AUC0–∞) showed an increase in amprenavir AUC0–∞ of 2.5-fold in the group with moderate cirrhosis and 4.5-fold in the group with severe cirrhosis compared with that in the control group of healthy volunteers (P < 0.05). AUC0–∞ was linearly related to the severity of liver disease, as assessed by the Child-Pugh score. Of the laboratory data used to calculate the Child-Pugh score, only the mean total bilirubin concentration showed a significant relationship with AUC0–∞. The relationship between the total bilirubin concentration and the AUC0–∞ of amprenavir was well characterized by a simple E max model, suggesting that the total bilirubin concentration may be a useful parameter for predicting the amprenavir AUC in subjects with hepatic insufficiency. Finally, the sera of cirrhotic subjects showed significant decreases in the levels of α1-acid glycoprotein, the primary plasma binding protein for amprenavir. On the basis of the results of this study, for an exposure equivalent to a clinical dose of 1,200 mg twice daily in subjects without cirrhosis, subjects with Child-Pugh scores of 5 to 8 should receive a twice-daily 450-mg dose of amprenavir, and subjects with Child-Pugh scores of 9 to 15 should receive a twice-daily 300-mg dose of amprenavir.

Serum Total Bilirubin Concentration Is Inversely Correlated with Framingham Risk Score in Koreans

2012 ◽  
Vol 43 (4) ◽  
pp. 288-293 ◽  
Author(s):  
Kwang-Min Kim ◽  
Bom-Taeck Kim ◽  
Sat-Byul Park ◽  
Doo-Yeoun Cho ◽  
Sang Hyeon Je ◽  
...  
Keyword(s):  
Risk Score ◽  
Framingham Risk Score ◽  
Total Bilirubin ◽  
Serum Total Bilirubin ◽  
Bilirubin Concentration ◽  
Framingham Risk ◽  
Total Bilirubin Concentration

OBSTRUCTIVE JAUNDICE COMPLICATING HEMOLYTIC DISEASE OF THE NEWBORN

PEDIATRICS ◽  
1956 ◽  
Vol 17 (4) ◽  
pp. 471-481
Author(s):  
Robert Stempfel ◽  
Birger Broman ◽  
Florencio E. Escardó ◽  
Rolf Zetterström
Keyword(s):  
Cord Blood ◽  
Biliary Obstruction ◽  
Total Bilirubin ◽  
Serum Proteins ◽  
Direct Bilirubin ◽  
Consecutive Series ◽  
Normal Infant ◽  
Hemolytic Disease ◽  
Bilirubin Concentration ◽  
Total Bilirubin Concentration

In a consecutive series of 83 Rh-isoimmunized infants, 7 had significantly elevated levels (greater than 1.0 mg./100 ml.) of direct bilirubin in the cord blood and developed obvious biliary obstruction. In no instance was a low concentration of direct bilirubin in the cord blood associated with the development of this condition. Serially determined electrophoretic separations of the serum proteins in 6 of the 7 patients revealed patterns not unlike those seen in the normal infant. In exchange transfusions calculated to remove 85 per cent of the infant's blood, the total bilirubin concentration of the serum was reduced 30 to 50 per cent in infants with and in those without biliary obstruction. However, for the purpose of maintaining the total bilirubin concentration below "critical" levels, it was found that exchange therapy is less efficient, from a temporal standpoint, in infants whose hemolytic disease is complicated by biliary obstruction. The cause of intrahepatic biliary obstruction is discussed and the prognostic value of the concentration of direct bilirubin in the cord blood of erythroblastotic infants is stressed.

Mechanized determination of the apparent unbound unconjugated bilirubin concentration in serum.

1979 ◽  
Vol 25 (8) ◽  
pp. 1444-1447 ◽  
Author(s):  
R P Wennberg ◽  
L F Rasmussen ◽  
C E Ahlfors ◽  
T Valaes
Keyword(s):  
Total Bilirubin ◽  
Gel Filtration ◽  
Operation Time ◽  
Serum Samples ◽  
Bilirubin Concentration ◽  
Coefficients Of Variation ◽  
Control Serum ◽  
Total Bilirubin Concentration ◽  
Unbound Bilirubin

Abstract The peroxidase method for determining the apparent unbound bilirubin concentration in serum has been automated by use of a programmable, computer-directed spectrophotometer. This mechanized assay determines the total bilirubin concentration and apparent unbound bilirubin concentration in serum samples and titrates the serum with bilirubin to estimate the effect of increasing total bilirubin concentrations on the apparent unbound bilirubin concentration. The entire analysis requires 0.1 mL of serum and 4 min operation time, as compared with about 30 min for the manual method. The coefficients of variation for determination of the apparent unbound bilirubin concentration in bilirubin-enriched commercial control serum were 2.8% within-day and 5.6% between-day. Bilirubin--albumin binding in serum samples from infants with severe hyperbilirubinemia was analyzed by the manual peroxidase method, the automated peroxidase method, and Sephadex gel filtration. Good correlation was found among all three methods.

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