scholarly journals The Expression Frequency of Androgen Receptor, c-erbB2 and CD117 in Acinar Adenocarcinoma of Prostate and Normal Prostate Tissue and It’s Clinical Importance

2015 ◽  
Vol 14 (4) ◽  
pp. 245-250
Author(s):  
Hülya Savaş Mutlu ◽  
Oğuz Aydın ◽  
Sancar Barış ◽  
Sevgi Canbaz ◽  
Filiz Karagöz
Keyword(s):  
Androgen Receptor ◽  
Clinical Importance ◽  
Prostate Tissue ◽  
Normal Prostate ◽  
Normal Prostate Tissue ◽  
Expression Frequency

Increased expression of androgen receptor (AR) and enzymes involved in androgen synthesis in metastatic prostate cancer: Targets for novel personalized therapies

2009 ◽  
Vol 27 (15_suppl) ◽  
pp. 5002-5002
Author(s):  
N. Mitsiades ◽  
N. Schultz ◽  
B. S. Taylor ◽  
H. Hieronymus ◽  
J. Satagopan ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Clinical Trials ◽  
Androgen Receptor ◽  
Normal Tissue ◽  
Expression Profiles ◽  
Prostate Tissue ◽  
Steroidogenic Enzymes ◽  
Normal Prostate ◽  
Androgen Synthesis ◽  
Normal Prostate Tissue

5002 Background: Androgen receptor (AR) signaling remains active in castration-resistant prostate cancer (CRPC) despite castrate levels of circulating androgens. This is indicated by continuous expression of androgen-responsive genes and is due to mechanisms that include: increased AR expression; AR mutations allowing promiscuous activation by alternative ligands; and increased intratumoral androgen levels, resulting from in situ steroidogenesis. Methods: Gene expression profiles of 30 normal prostate tissue samples, 131 primary prostate carcinomas (PCas) and 16 metastatic PCas, generated using Affymetrix Exon arrays, were interrogated for levels of 40 mRNAs encoding AR, SHBG, 28 enzymes involved in androgen synthesis and 10 enzymes involved in androgen inactivation. For individual tumors, a transcript was considered to be overexpressed or underexpressed when its levels were >2 SDs higher or lower, respectively, than its average levels in normal tissue. Results: Metastatic PCas expressed higher average transcript levels for AR and several steroidogenic enzymes, including SRD5A1 and SRD5A3, than primary PCas and normal prostate tissue. Expression of SRD5A2, CYP3A4, CYP3A5, and CYP3A7 mRNAs was decreased both in primary and metastatic tumors compared to normal prostate tissue. In analysis involving AR and 28 steroidogenic transcripts in individual tumors, all (16/16) metastatic PCas overexpressed at least one transcript (range: 2–14, median: 5 transcripts) compared to normal tissue, while 100/131 (76%) primary PCas overexpressed at least one transcript (range: 2–16, median: 2). Conclusions: Metastatic PCas overexpress AR and several steroidogenic enzymes, while they express lower levels of the androgen-inactivating enzymes CYP3A4, CYP3A5, and CYP3A7. These data highlight the role of AR and the androgen synthetic pathway as a therapeutic target in CRPC. Novel antiandrogens (MDV3100) and CYP17 inhibitors (abiraterone) are already in clinical trials in CRPC. Overexpression of AR or steroidogenic enzymes may serve as a biomarker (e.g. by detection via RT-PCR in circulating tumor cells) to predict for sensitivity to these agents and guide patient selection for participation in clinical trials. No significant financial relationships to disclose.

scholarly journals Epithelial Phenotypes in the Developing Human Prostate

2007 ◽  
Vol 55 (9) ◽  
pp. 885-890 ◽  
Author(s):  
Guy Letellier ◽  
Marie-José Perez ◽  
Mokrane Yacoub ◽  
Pierre Levillain ◽  
Olivier Cussenot ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Growth Factor ◽  
Regulatory Function ◽  
Basal Cells ◽  
Normal Prostate ◽  
Prostate Development ◽  
Proliferation And Apoptosis ◽  
Normal Prostate Tissue ◽  
Epidermal Growth ◽  
Luminal Cells

An intermediate population has been identified among prostate glands called transiently amplifying (TA) cells, which are characterized by coexpression of basal and luminal cytokeratins (CKs), high proliferation, and lack of p27 expression. These cells are rare in the normal adult prostate and increase in pretumoral conditions, but their importance in the developing gland remains unknown. We analyzed fetal prostates for the expression of CKs (5/6, 18, 19) and factors involved in proliferation and apoptosis: p63, Ki67, p27, epidermal growth factor (EGFR), Bcl2, androgen receptor (AR). Immunostaining was performed on a tissue microarray, including 40 prostates from fetuses aged 13-42 weeks and normal prostate tissue from 10 adults. In both solid buds and the basal compartment of canalized glands, cells expressed p63, CK5/6, CK19, CK18, BCL2, EGFR and were p27 negative. Luminal cells of fetal canalized glands continue to express CK19, EGFR, and BCL2, without p27 expression. In contrast, adult epithelial luminal cells showed diffuse AR and p27 expression, without CK19, BCL2, and EGFR staining. Proliferation was high and diffuse in fetal glands and rare and restricted to basal cells in adult glands. These results indicate that most fetal epithelial prostatic cells exhibit the phenotype of TA cells, suggesting their regulatory function in prostate development.

scholarly journals Ratio of Tumor to Normal Prostate Tissue Apparent Diffusion Coefficient as a Method for Quantifying DWI of the Prostate

2015 ◽  
Vol 205 (6) ◽  
pp. W585-W593 ◽  
Author(s):  
Tristan Barrett ◽  
Andrew N. Priest ◽  
Edward M. Lawrence ◽  
Debra A. Goldman ◽  
Anne Y. Warren ◽  
...  
Keyword(s):  
Diffusion Coefficient ◽  
Apparent Diffusion Coefficient ◽  
Prostate Tissue ◽  
Normal Prostate ◽  
Apparent Diffusion ◽  
Normal Prostate Tissue

scholarly journals An expanded variant list and assembly annotation identifies multiple novel coding and noncoding genes for prostate cancer risk using a normal prostate tissue eQTL data set

PLoS ONE ◽  
2019 ◽  
Vol 14 (4) ◽  
pp. e0214588
Author(s):  
Melissa S. DeRycke ◽  
Melissa C. Larson ◽  
Asha A. Nair ◽  
Shannon K. McDonnell ◽  
Amy J. French ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cancer Risk ◽  
Prostate Cancer Risk ◽  
Prostate Tissue ◽  
Data Set ◽  
Normal Prostate ◽  
Normal Prostate Tissue ◽  
Eqtl Data

Molecular changes in histopatologically normal prostate tissue adjacent to cancer

2008 ◽  
Vol 6 (9) ◽  
pp. 109-110
Author(s):  
A. Fiszer-Kierzkowska ◽  
E. Malusecka ◽  
M. Jarzab ◽  
M. Gawkowska-Suwinska ◽  
J. Rembak-Szynkiewicz ◽  
...  
Keyword(s):  
Prostate Tissue ◽  
Normal Prostate ◽  
Molecular Changes ◽  
Normal Prostate Tissue

Differential gene expression in prostate tissue according to vasectomy.

2016 ◽  
Vol 34 (2_suppl) ◽  
pp. 298-298
Author(s):  
Kathryn M Wilson ◽  
Travis Gerke ◽  
Ericka Ebot ◽  
Jennifer A Sinnott ◽  
Jennifer R. Rider ◽  
...  
Keyword(s):  
Gene Expression ◽  
Prostate Cancer ◽  
Cancer Diagnosis ◽  
Enrichment Analysis ◽  
Gene Set Enrichment Analysis ◽  
Prostate Tissue ◽  
Gene Set Enrichment ◽  
Normal Prostate ◽  
Gene Sets ◽  
Normal Prostate Tissue

298 Background: We previously found that vasectomy was associated with an increased risk of prostate cancer, and particularly, risk of lethal prostate cancer in the Health Professionals Follow-up Study (HPFS). However, the possible biological basis for this finding is unclear. In this study, we explored possible biological mechanisms by assessing differences in gene expression in the prostate tissue of men with and without a history of vasectomy prostate cancer diagnosis. Methods: Within the HPFS, vasectomy data and gene expression data (20,254 genes) was available from archival tumor tissue from 263 cases, 124 of whom also had data for adjacent normal tissue. To relate expression of individual genes to vasectomy we used linear regression adjusting for age and year at diagnosis. We ran gene set enrichment analysis to identify pathways of genes associated with vasectomy. Results: Among 263 cases, 67 (25%) reported a vasectomy prior to cancer diagnosis. Mean age at diagnosis was 66 years among men without and 65 years among men with vasectomy. Median time between vasectomy and prostate cancer diagnosis was 25 years. Gene expression in tumor tissue was not associated with vasectomy status. In adjacent normal tissue, three individual genes were associated with vasectomy with Bonferroni-corrected p-values of < 0.10: RAPGEF6, OR4C3, and SLC35F4. Gene set enrichment analysis found five pathways upregulated and seven pathways downregulated in men with vasectomy compared to those without in normal prostate tissue with a FDR < 0.05. Upregulated pathways included several immune-related gene sets and G-protein-coupled receptor gene sets. Conclusions: We identified significant differences in gene expression profiles in normal prostate tissue according to vasectomy status among men treated for prostate cancer. The fact that such differences existed several decades after vasectomy provides support for the idea that vasectomy may play a role in the etiology of prostate cancer.

scholarly journals Multispectral Photoacoustic Imaging of Prostate Cancer: Preliminary Ex-vivo Results

2013 ◽  
Vol 3 ◽  
pp. 41 ◽  
Author(s):  
Vikram S. Dogra ◽  
Bhargava K. Chinni ◽  
Keerthi S. Valluru ◽  
Jean V. Joseph ◽  
Ahmed Ghazi ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Predictive Value ◽  
Ex Vivo ◽  
Prostate Tissue ◽  
Human Prostate ◽  
Normal Prostate ◽  
Mean Intensity ◽  
Preliminary Results ◽  
Normal Prostate Tissue ◽  
Malignant Prostate

Objective: The objective of this study is to validate if ex-vivo multispectral photoacoustic (PA) imaging can differentiate between malignant prostate tissue, benign prostatic hyperplasia (BPH), and normal human prostate tissue. Materials and Methods: Institutional Review Board's approval was obtained for this study. A total of 30 patients undergoing prostatectomy for biopsy-confirmed prostate cancer were included in this study with informed consent. Multispectral PA imaging was performed on surgically excised prostate tissue and chromophore images that represent optical absorption of deoxyhemoglobin (dHb), oxyhemoglobin (HbO2), lipid, and water were reconstructed. After the imaging procedure is completed, malignant prostate, BPH and normal prostate regions were marked by the genitourinary pathologist on histopathology slides and digital images of marked histopathology slides were obtained. The histopathology images were co-registered with chromophore images. Region of interest (ROI) corresponding to malignant prostate, BPH and normal prostate were defined on the chromophore images. Pixel values within each ROI were then averaged to determine mean intensities of dHb, HbO2, lipid, and water. Results: Our preliminary results show that there is statistically significant difference in mean intensity of dHb (P < 0.0001) and lipid (P = 0.0251) between malignant prostate and normal prostate tissue. There was difference in mean intensity of dHb (P < 0.0001) between malignant prostate and BPH. Sensitivity, specificity, positive predictive value, and negative predictive value of our imaging system were found to be 81.3%, 96.2%, 92.9% and 89.3% respectively. Conclusion: Our preliminary results of ex-vivo human prostate study suggest that multispectral PA imaging can differentiate between malignant prostate, BPH and normal prostate tissue.

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