scholarly journals In Vivo Effects of Naringenin and Lead on Rat Erythrocyte Carbonic Anhydrase Enzyme

2017 ◽  
Vol 14 (1) ◽  
pp. 9-12 ◽  
Author(s):  
Müslüm KUZU ◽  
Ahmet ÖZKAYA ◽  
Zafer ŞAHİN ◽  
Üzeyir DAĞ ◽  
Veysel ÇOMAKLI ◽  
...  
1978 ◽  
Vol 100 (9) ◽  
pp. 2883-2885 ◽  
Author(s):  
Y. Pocker ◽  
J. E. Meany ◽  
Brain C. Davis ◽  
James Arrigoni ◽  
John E. Stein

Blood ◽  
1976 ◽  
Vol 47 (6) ◽  
pp. 883-897
Author(s):  
SH Boyer ◽  
AN Noyes ◽  
ML Boyer

Red cell lysis in isotonic solutions containing NH4Cl, NH4HCO3, and a carbonic anhydrase enzyme inhibitor (acetazolamide) is a function of erythrocyte enzyme activity and permeability of cells to the inhibitor. Erythrocyte carbonic anhydrase activity is at least fivefold greater and acetazolamide permeability about tenfold less for adults than for newborns. In this setting, greater than 99.9% of red cells from adults can be hemolyzed at a time when greater than 25% of those from newborns remain intact. This easily applied method may be useful when antenatal diagnosis of hemoglobinopathies is otherwise precluded by contaimination with maternal erythrocytes. The feasibility of differential hemolysis via NH4Cl--HCO3-mediated, acetazolamide- modulated reactions is shown by the successful isolation of the few fetal-origin erythrocytes present in grossly nonbloody amniotic fluids and, in one instance, by approximately 3300-fold enrichment of apparently authentic fetal-origin red cells from the arm blood of a woman in her 18th wk of pregnancy.


2004 ◽  
Vol 27 (5) ◽  
pp. 613-616 ◽  
Author(s):  
İlhami Gülçin ◽  
Şükrü Beydemir ◽  
Mehmet Emin Büyükokuroğlu

Blood ◽  
1976 ◽  
Vol 47 (6) ◽  
pp. 883-897 ◽  
Author(s):  
SH Boyer ◽  
AN Noyes ◽  
ML Boyer

Abstract Red cell lysis in isotonic solutions containing NH4Cl, NH4HCO3, and a carbonic anhydrase enzyme inhibitor (acetazolamide) is a function of erythrocyte enzyme activity and permeability of cells to the inhibitor. Erythrocyte carbonic anhydrase activity is at least fivefold greater and acetazolamide permeability about tenfold less for adults than for newborns. In this setting, greater than 99.9% of red cells from adults can be hemolyzed at a time when greater than 25% of those from newborns remain intact. This easily applied method may be useful when antenatal diagnosis of hemoglobinopathies is otherwise precluded by contaimination with maternal erythrocytes. The feasibility of differential hemolysis via NH4Cl--HCO3-mediated, acetazolamide- modulated reactions is shown by the successful isolation of the few fetal-origin erythrocytes present in grossly nonbloody amniotic fluids and, in one instance, by approximately 3300-fold enrichment of apparently authentic fetal-origin red cells from the arm blood of a woman in her 18th wk of pregnancy.


2007 ◽  
Vol 30 (12) ◽  
pp. 2257-2261 ◽  
Author(s):  
Taha Abdulkadir Çoban ◽  
Şükrü Beydemir ◽  
İlhami Gülçin ◽  
Deniz Ekinci

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